Restriction map for
pF1KB7314
(Circular) (Six-base) MAPSORT of: pF1KB7314.seq Check: 5214 from: 1 to: 6350
pF1KB7314 6350 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3203 3203
Size: 6350
AccI GT'mk_AC
Cuts at: 515 2552 3388 515
Size: 2037 836 3477
Fragments arranged by size:
3477 2037 836
Acc65I G'GTAC_C
Cuts at: 3366 3366
Size: 6350
AclI AA'CG_TT
Cuts at: 1245 6190 1245
Size: 4945 1405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 2393 4175 4607 5354 5622 380
Size: 2013 1782 432 747 268 1108
Fragments arranged by size:
2013 1782 1108 747 432 268
AflIII A'CryG_T
Cuts at: 3702 4806 5783 3702
Size: 1104 977 4269
Fragments arranged by size:
4269 1104 977
AgeI A'CCGG_T
Cuts at: 4738 4738
Size: 6350
AhdI GACnn_n'nnGTC
Cuts at: 648 648
Size: 6350
AlwNI CAG_nnn'CTG
Cuts at: 2227 2961 3655 5222 2227
Size: 734 694 1567 3355
Fragments arranged by size:
3355 1567 734 694
ApaLI G'TGCA_C
Cuts at: 5120 5120
Size: 6350
ApoI r'AATT_y
Cuts at: 602 716 1282 1520 1703 2259 3354 5703 5959
Size: 114 566 238 183 556 1095 2349 256
Cuts at: 5959 602
Size: 993
Fragments arranged by size:
2349 1095 993 566 556 256 238 183
114
AseI AT'TA_AT
Cuts at: 20 2266 20
Size: 2246 4104
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
AvaI C'yCGr_G
Cuts at: 3341 3370 3341
Size: 29 6321
AvrII C'CTAG_G
Cuts at: 1170 1170
Size: 6350
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1586 1619 1586
Size: 33 6317
BamHI G'GATC_C
Cuts at: 2985 3375 2985
Size: 390 5960
BanI G'GyrC_C
Cuts at: 295 2133 3191 3366 3780 4028 4063 295
Size: 1838 1058 175 414 248 35 2582
Fragments arranged by size:
2582 1838 1058 414 248 175 35
BanII G_rGCy'C
Cuts at: 623 2542 2877 3364 4394 623
Size: 1919 335 487 1030 2579
Fragments arranged by size:
2579 1919 1030 487 335
BbeI G_GCGC'C
Cuts at: 299 3784 4032 299
Size: 3485 248 2617
Fragments arranged by size:
3485 2617 248
BbsI GAAGACnn'nnnn_
Cuts at: 313 313
Size: 6350
BbvCI CC'TCA_GC
Cuts at: 2282 3163 3297 2282
Size: 881 134 5335
Fragments arranged by size:
5335 881 134
BciVI GTATCCnnnnn_n'
Cuts at: 4248 5009 6350 4248
Size: 761 1341 4248
Fragments arranged by size:
4248 1341 761
BclI T'GATC_A
Cuts at: 2382 3870 2382
Size: 1488 4862
BfrBI ATG'CAT
Cuts at: 532 855 532
Size: 323 6027
BglII A'GATC_T
Cuts at: 2575 2588 2886 3028 3865 5902 2575
Size: 13 298 142 837 2037 3023
Fragments arranged by size:
3023 2037 837 298 142 13
BlpI GC'TnA_GC
Cuts at: 3462 3462
Size: 6350
Bme1580I G_kGCm'C
Cuts at: 3975 4068 5124 3975
Size: 93 1056 5201
Fragments arranged by size:
5201 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1947 1952 2615 2991 3562 3643 3978 6122 1947
Size: 5 663 376 571 81 335 2144 2175
Fragments arranged by size:
2175 2144 663 571 376 335 81 5
BmtI G_CTAG'C
Cuts at: 2570 2570
Size: 6350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4717 4717
Size: 6350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1643 2177 2891 1643
Size: 534 714 5102
Fragments arranged by size:
5102 714 534
Bpu10I CC'TnA_GC
Cuts at: 1502 1874 2282 3163 3297 1502
Size: 372 408 881 134 4555
Fragments arranged by size:
4555 881 408 372 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1187 3526 4405 4897 5195 1187
Size: 2339 879 492 298 2342
Fragments arranged by size:
2342 2339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6054 6054
Size: 6350
BsaAI yAC'GTr
Cuts at: 2860 3703 2860
Size: 843 5507
BsaBI GATnn'nnATC
Cuts at: 6 3864 5901 6
Size: 3858 2037 455
Fragments arranged by size:
3858 2037 455
BsaHI Gr'CG_yC
Cuts at: 296 3781 4029 4731 6227 296
Size: 3485 248 702 1496 419
Fragments arranged by size:
3485 1496 702 419 248
BsaWI w'CCGG_w
Cuts at: 3539 4060 4738 5012 5159 5570 3539
Size: 521 678 274 147 411 4319
Fragments arranged by size:
4319 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2418 2448 2418
Size: 30 6320
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 220
Size: 6350
BseYI C'CCAG_C
Cuts at: 580 2361 2523 3313 3775 4533 5110 5606 580
Size: 1781 162 790 462 758 577 496 1324
Fragments arranged by size:
1781 1324 790 758 577 496 462 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1070 797
Size: 273 6077
BsiEI CG_ry'CG
Cuts at: 104 3892 3938 5146 104
Size: 3788 46 1208 1308
Fragments arranged by size:
3788 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1593 2542 2877 3364 4142 4332 5124 1593
Size: 949 335 487 778 190 792 2819
Fragments arranged by size:
2819 949 792 778 487 335 190
BsmI GAATG_Cn'
Cuts at: 489 3104 489
Size: 2615 3735
BsmBI CGTCTCn'nnnn_
Cuts at: 268 1617 268
Size: 1349 5001
Bsp1286I G_dGCh'C
Cuts at: 623 1593 2542 2877 3364 3975 4068 4142 4332
Size: 970 949 335 487 611 93 74 190
Cuts at: 4332 4394 5124 623
Size: 62 730 1849
Fragments arranged by size:
1849 970 949 730 611 487 335 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5570 5570
Size: 6350
BspHI T'CATG_A
Cuts at: 285 5526 285
Size: 5241 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1790 2564 3203 3400 3614 3916 4297 1790
Size: 774 639 197 214 302 381 3843
Fragments arranged by size:
3843 774 639 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 1873 4642 4696 6180 6345 50
Size: 1823 2769 54 1484 165 55
Fragments arranged by size:
2769 1823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2325 3109 4262 2325
Size: 784 1153 4413
Fragments arranged by size:
4413 1153 784
BsrFI r'CCGG_y
Cuts at: 4348 4529 4738 4348
Size: 181 209 5960
Fragments arranged by size:
5960 209 181
BsrGI T'GTAC_A
Cuts at: 1131 2827 1131
Size: 1696 4654
BssSI C'ACGA_G
Cuts at: 4621 4979 4621
Size: 358 5992
BstAPI GCAn_nnn'nTGC
Cuts at: 439 3727 439
Size: 3288 3062
BstBI TT'CG_AA
Cuts at: 4711 4711
Size: 6350
BstXI CCAn_nnnn'nTGG
Cuts at: 2913 3313 2913
Size: 400 5950
BstYI r'GATC_y
Cuts at: 1 867 936 2125 2575 2588 2886 2985 3028
Size: 866 69 1189 450 13 298 99 43
Cuts at: 3028 3375 3842 3865 4200 4446 5458 5544 5556
Size: 347 467 23 335 246 1012 86 12
Cuts at: 5556 5902 1
Size: 346 449
Fragments arranged by size:
1189 1012 866 467 450 449 347 346
335 298 246 99 86 69 43 23
13 12
Bsu36I CC'TnA_GG
Cuts at: 2070 3279 2070
Size: 1209 5141
BtgI C'CryG_G
Cuts at: 1143 1846 2244 1143
Size: 703 398 5249
Fragments arranged by size:
5249 703 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4300 4481 4644 4300
Size: 181 163 6006
Fragments arranged by size:
6006 181 163
BtsI GCAGTG_nn'
Cuts at: 128 1549 3121 3593 128
Size: 1421 1572 472 2885
Fragments arranged by size:
2885 1572 1421 472
DraI TTT'AAA
Cuts at: 659 1421 3349 659
Size: 762 1928 3660
Fragments arranged by size:
3660 1928 762
DraIII CAC_nnn'GTG
Cuts at: 1588 1588
Size: 6350
DrdI GACnn_nn'nnGTC
Cuts at: 4056 4914 4056
Size: 858 5492
EaeI y'GGCC_r
Cuts at: 948 1317 3252 3935 4109 4500 4527 5872 948
Size: 369 1935 683 174 391 27 1345 1426
Fragments arranged by size:
1935 1426 1345 683 391 369 174 27
EagI C'GGCC_G
Cuts at: 3935 3935
Size: 6350
EarI CTCTTCn'nnn_
Cuts at: 197 271 1835 2403 2412 4373 4583 197
Size: 74 1564 568 9 1961 210 1964
Fragments arranged by size:
1964 1961 1564 568 210 74 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 3122 4558 4868 5014 6160 190
Size: 2932 1436 310 146 1146 380
Fragments arranged by size:
2932 1436 1146 380 310 146
EcoICRI GAG'CTC
Cuts at: 2540 2875 3362 2540
Size: 335 487 5528
Fragments arranged by size:
5528 487 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 1643 2177 2393 2891 4175 4607 5354 5622
Size: 1263 534 216 498 1284 432 747 268
Cuts at: 5622 380
Size: 1108
Fragments arranged by size:
1284 1263 1108 747 534 498 432 268
216
EcoO109I rG'GnC_Cy
Cuts at: 1912 3489 1912
Size: 1577 4773
EcoRI G'AATT_C
Cuts at: 1520 3354 1520
Size: 1834 4516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 583 4369 583
Size: 3786 2564
FspI TGC'GCA
Cuts at: 120 4131 120
Size: 4011 2339
HaeII r_GCGC'y
Cuts at: 244 299 3784 4032 5054 6033 244
Size: 55 3485 248 1022 979 561
Fragments arranged by size:
3485 1022 979 561 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 605 634 637 666 744 776 3103 3135 4192
Size: 29 3 29 78 32 2327 32 1057
Cuts at: 4192 4222 4224 4254 605
Size: 30 2 30 2701
Fragments arranged by size:
2701 2327 1057 78 32 32 30 30
29 29 3 2
HincII GTy'rAC
Cuts at: 576 3389 576
Size: 2813 3537
KasI G'GCGC_C
Cuts at: 295 3780 4028 295
Size: 3485 248 2617
Fragments arranged by size:
3485 2617 248
KpnI G_GTAC'C
Cuts at: 3370 3370
Size: 6350
MfeI C'AATT_G
Cuts at: 1135 3041 1135
Size: 1906 4444
MluI A'CGCG_T
Cuts at: 5783 5783
Size: 6350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 829 5021 5205 395
Size: 434 4192 184 1540
Fragments arranged by size:
4192 1540 434 184
MscI TGG'CCA
Cuts at: 950 1319 3254 4111 950
Size: 369 1935 857 3189
Fragments arranged by size:
3189 1935 857 369
MslI CAynn'nnrTG
Cuts at: 290 740 1148 4339 4466 290
Size: 450 408 3191 127 2174
Fragments arranged by size:
3191 2174 450 408 127
MspA1I CmG'CkG
Cuts at: 142 1443 2365 3461 3775 4135 5148 5393 142
Size: 1301 922 1096 314 360 1013 245 1099
Fragments arranged by size:
1301 1099 1096 1013 922 360 314 245
NaeI GCC'GGC
Cuts at: 4531 4531
Size: 6350
NarI GG'CG_CC
Cuts at: 296 3781 4029 296
Size: 3485 248 2617
Fragments arranged by size:
3485 2617 248
NcoI C'CATG_G
Cuts at: 1143 1846 2244 1143
Size: 703 398 5249
Fragments arranged by size:
5249 703 398
NdeI CA'TA_TG
Cuts at: 780 2936 780
Size: 2156 4194
NgoMIV G'CCGG_C
Cuts at: 4529 4529
Size: 6350
NheI G'CTAG_C
Cuts at: 2566 2566
Size: 6350
NsiI A_TGCA'T
Cuts at: 534 857 534
Size: 323 6027
NspI r_CATG'y
Cuts at: 1153 2648 3403 3903 4810 1153
Size: 1495 755 500 907 2693
Fragments arranged by size:
2693 1495 907 755 500
PciI A'CATG_T
Cuts at: 4806 4806
Size: 6350
PfoI T'CCnGG_A
Cuts at: 3077 3077
Size: 6350
PmeI GTTT'AAAC
Cuts at: 3349 3349
Size: 6350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5489 5521 5489
Size: 32 6318
PpuMI rG'GwC_Cy
Cuts at: 1912 1912
Size: 6350
PshAI GACnn'nnGTC
Cuts at: 260 260
Size: 6350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 628 660 628
Size: 32 6318
PstI C_TGCA'G
Cuts at: 1677 1787 3128 3397 4082 1677
Size: 110 1341 269 685 3945
Fragments arranged by size:
3945 1341 685 269 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6350
PvuII CAG'CTG
Cuts at: 1443 2365 3775 4135 1443
Size: 922 1410 360 3658
Fragments arranged by size:
3658 1410 922 360
RsrII CG'GwC_CG
Cuts at: 4545 4545
Size: 6350
SacI G_AGCT'C
Cuts at: 2542 2877 3364 2542
Size: 335 487 5528
Fragments arranged by size:
5528 487 335
SalI G'TCGA_C
Cuts at: 3387 3387
Size: 6350
SapI GCTCTTCn'nnn_
Cuts at: 1835 4373 4583 1835
Size: 2538 210 3602
Fragments arranged by size:
3602 2538 210
SbfI CC_TGCA'GG
Cuts at: 3397 3397
Size: 6350
ScaI AGT'ACT
Cuts at: 5867 5867
Size: 6350
SfcI C'TryA_G
Cuts at: 33 1288 1673 1783 3124 3393 4078 5071 5262
Size: 1255 385 110 1341 269 685 993 191
Cuts at: 5262 33
Size: 1121
Fragments arranged by size:
1341 1255 1121 993 685 385 269 191
110
SfoI GGC'GCC
Cuts at: 297 3782 4030 297
Size: 3485 248 2617
Fragments arranged by size:
3485 2617 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6350
SmaI CCC'GGG
Cuts at: 3343 3372 3343
Size: 29 6321
SmlI C'TyrA_G
Cuts at: 1202 3505 4420 4912 5174 1202
Size: 2303 915 492 262 2378
Fragments arranged by size:
2378 2303 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6350
SphI G_CATG'C
Cuts at: 1153 2648 3403 3903 1153
Size: 1495 755 500 3600
Fragments arranged by size:
3600 1495 755 500
SspI AAT'ATT
Cuts at: 2355 2355
Size: 6350
StyI C'CwwG_G
Cuts at: 504 596 1143 1170 1846 2129 2244 3484 3838
Size: 92 547 27 676 283 115 1240 354
Cuts at: 3838 504
Size: 3016
Fragments arranged by size:
3016 1240 676 547 354 283 115 92
27
SwaI ATTT'AAAT
Cuts at: 1421 1421
Size: 6350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2375 2778 3933 4066 4525 4734 2375
Size: 403 1155 133 459 209 3991
Fragments arranged by size:
3991 1155 459 403 209 133
TatI w'GTAC_w
Cuts at: 697 1131 1341 1714 2827 5865 697
Size: 434 210 373 1113 3038 1182
Fragments arranged by size:
3038 1182 1113 434 373 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3309 3341 5867 5899 3309
Size: 32 2526 32 3760
Fragments arranged by size:
3760 2526 32 32
Tth111I GACn'n_nGTC
Cuts at: 4147 4147
Size: 6350
XbaI T'CTAG_A
Cuts at: 3381 3381
Size: 6350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2319 3845 2319
Size: 1526 4824
XmaI C'CCGG_G
Cuts at: 3341 3370 3341
Size: 29 6321
XmnI GAAnn'nnTTC
Cuts at: 806 806
Size: 6350
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
BcgI BglI BmgBI BsiWI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI NotI
NruI PacI PasI PflMI PmlI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
XhoI ZraI