Restriction map for
pF1KB7312
(Circular) (Six-base) MAPSORT of: pF1KB7312.seq Check: 3260 from: 1 to: 6290
pF1KB7312 6290 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2619 2619
Size: 6290
AatII G_ACGT'C
Cuts at: 2723 2723
Size: 6290
AccI GT'mk_AC
Cuts at: 2147 2723 3328 2147
Size: 576 605 5109
Fragments arranged by size:
5109 605 576
Acc65I G'GTAC_C
Cuts at: 3306 3306
Size: 6290
AclI AA'CG_TT
Cuts at: 6130 6130
Size: 6290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 4115 4547 5294 5562 1690
Size: 2425 432 747 268 2418
Fragments arranged by size:
2425 2418 747 432 268
AflIII A'CryG_T
Cuts at: 3642 4746 5723 3642
Size: 1104 977 4209
Fragments arranged by size:
4209 1104 977
AgeI A'CCGG_T
Cuts at: 4678 4678
Size: 6290
AhdI GACnn_n'nnGTC
Cuts at: 1279 1279
Size: 6290
AleI CACnn'nnGTG
Cuts at: 499 788 1756 499
Size: 289 968 5033
Fragments arranged by size:
5033 968 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3097 3097
Size: 6290
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 6258
AlwNI CAG_nnn'CTG
Cuts at: 408 1176 3595 5162 408
Size: 768 2419 1567 1536
Fragments arranged by size:
2419 1567 1536 768
ApaI G_GGCC'C
Cuts at: 927 1776 2176 927
Size: 849 400 5041
Fragments arranged by size:
5041 849 400
ApaLI G'TGCA_C
Cuts at: 5060 5060
Size: 6290
ApoI r'AATT_y
Cuts at: 3294 5643 5899 3294
Size: 2349 256 3685
Fragments arranged by size:
3685 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6290
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6290
AvaI C'yCGr_G
Cuts at: 738 1373 1613 2032 2400 2917 3123 3239 3310
Size: 635 240 419 368 517 206 116 71
Cuts at: 3310 738
Size: 3718
Fragments arranged by size:
3718 635 517 419 368 240 206 116
71
AvrII C'CTAG_G
Cuts at: 2754 2754
Size: 6290
BamHI G'GATC_C
Cuts at: 446 1397 2297 3315 446
Size: 951 900 1018 3421
Fragments arranged by size:
3421 1018 951 900
BanI G'GyrC_C
Cuts at: 1650 2565 2648 3306 3720 3968 4003 1650
Size: 915 83 658 414 248 35 3937
Fragments arranged by size:
3937 915 658 414 248 83 35
BanII G_rGCy'C
Cuts at: 116 515 927 1295 1498 1776 1816 2039 2176
Size: 399 412 368 203 278 40 223 137
Cuts at: 2176 2924 2974 3161 3304 4334 116
Size: 748 50 187 143 1030 2072
Fragments arranged by size:
2072 1030 748 412 399 368 278 223
203 187 143 137 50 40
BbeI G_GCGC'C
Cuts at: 2569 2652 3724 3972 2569
Size: 83 1072 248 4887
Fragments arranged by size:
4887 1072 248 83
BbvCI CC'TCA_GC
Cuts at: 895 3031 895
Size: 2136 4154
BciVI GTATCCnnnnn_n'
Cuts at: 4188 4949 6290 4188
Size: 761 1341 4188
Fragments arranged by size:
4188 1341 761
BclI T'GATC_A
Cuts at: 3810 3810
Size: 6290
BglI GCCn_nnn'nGGC
Cuts at: 2527 2527
Size: 6290
BglII A'GATC_T
Cuts at: 3805 5842 3805
Size: 2037 4253
BlpI GC'TnA_GC
Cuts at: 409 509 3402 409
Size: 100 2893 3297
Fragments arranged by size:
3297 2893 100
Bme1580I G_kGCm'C
Cuts at: 143 927 1655 1776 1786 2176 3915 4008 5064
Size: 784 728 121 10 390 1739 93 1056
Cuts at: 5064 143
Size: 1369
Fragments arranged by size:
1739 1369 1056 784 728 390 121 93
10
BmgBI CAC'GTC
Cuts at: 984 2853 984
Size: 1869 4421
BmrI ACTGGGnnnn_n'
Cuts at: 1563 3055 3191 3502 3583 3918 6062 1563
Size: 1492 136 311 81 335 2144 1791
Fragments arranged by size:
2144 1791 1492 335 311 136 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 663 1311 4657 663
Size: 648 3346 2296
Fragments arranged by size:
3346 2296 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 388 442 717 1015 1423 1915 2323 2812
Size: 3 54 275 298 408 492 408 489
Cuts at: 2812 2884 385
Size: 72 3791
Fragments arranged by size:
3791 492 489 408 408 298 275 72
54 3
Bpu10I CC'TnA_GC
Cuts at: 895 1568 2246 2770 3031 895
Size: 673 678 524 261 4154
Fragments arranged by size:
4154 678 673 524 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 3466 4345 4837 5135 1093
Size: 2373 879 492 298 2248
Fragments arranged by size:
2373 2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 325 5994 325
Size: 5669 621
BsaAI yAC'GTr
Cuts at: 276 879 1390 3643 276
Size: 603 511 2253 2923
Fragments arranged by size:
2923 2253 603 511
BsaBI GATnn'nnATC
Cuts at: 6 3804 5841 6
Size: 3798 2037 455
Fragments arranged by size:
3798 2037 455
BsaHI Gr'CG_yC
Cuts at: 358 1356 2050 2506 2519 2566 2649 2720 3721
Size: 998 694 456 13 47 83 71 1001
Cuts at: 3721 3969 4671 6167 358
Size: 248 702 1496 481
Fragments arranged by size:
1496 1001 998 702 694 481 456 248
83 71 47 13
BsaWI w'CCGG_w
Cuts at: 305 1993 3479 4000 4678 4952 5099 5510 305
Size: 1688 1486 521 678 274 147 411 1085
Fragments arranged by size:
1688 1486 1085 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 460 490 736 766 1431 1461 1688 1718 2083
Size: 30 246 30 665 30 227 30 365
Cuts at: 2083 2113 2296 2326 2608 2638 460
Size: 30 183 30 282 30 4112
Fragments arranged by size:
4112 665 365 282 246 227 183 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 442 1303 2203 3019 439
Size: 3 861 900 816 3710
Fragments arranged by size:
3710 900 861 816 3
BseYI C'CCAG_C
Cuts at: 1072 1097 1328 1465 2228 2318 2413 2543 2966
Size: 25 231 137 763 90 95 130 423
Cuts at: 2966 3133 3194 3715 4473 5050 5546 1072
Size: 167 61 521 758 577 496 1816
Fragments arranged by size:
1816 763 758 577 521 496 423 231
167 137 130 95 90 61 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1831 3085 364
Size: 1467 1254 3569
Fragments arranged by size:
3569 1467 1254
BsiEI CG_ry'CG
Cuts at: 104 290 974 1023 2573 3020 3832 3878 5086
Size: 186 684 49 1550 447 812 46 1208
Cuts at: 5086 104
Size: 1308
Fragments arranged by size:
1550 1308 1208 812 684 447 186 49
46
BsiHKAI G_wGCw'C
Cuts at: 470 515 785 2039 2090 2488 3304 4082 4272
Size: 45 270 1254 51 398 816 778 190
Cuts at: 4272 5064 470
Size: 792 1696
Fragments arranged by size:
1696 1254 816 792 778 398 270 190
51 45
Bsp1286I G_dGCh'C
Cuts at: 116 143 470 515 785 927 1295 1498 1655
Size: 27 327 45 270 142 368 203 157
Cuts at: 1655 1776 1786 1816 2039 2090 2176 2488 2924
Size: 121 10 30 223 51 86 312 436
Cuts at: 2924 2974 3161 3304 3915 4008 4082 4272 4334
Size: 50 187 143 611 93 74 190 62
Cuts at: 4334 5064 116
Size: 730 1342
Fragments arranged by size:
1342 730 611 436 368 327 312 270
223 203 190 187 157 143 142 121
93 86 74 62 51 50 45 30
27 10
BspEI T'CCGG_A
Cuts at: 1993 5510 1993
Size: 3517 2773
BspHI T'CATG_A
Cuts at: 5466 5466
Size: 6290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1650 1755 2466 2619 3052 3340 3554 3856 4237
Size: 105 711 153 433 288 214 302 381
Cuts at: 4237 1650
Size: 3703
Fragments arranged by size:
3703 711 433 381 302 288 214 153
105
BsrBI CCG'CTC
Cuts at: 50 166 190 2774 4582 4636 6120 6285 50
Size: 116 24 2584 1808 54 1484 165 55
Fragments arranged by size:
2584 1808 1484 165 116 55 54 24
BsrDI GCAATG_nn'
Cuts at: 1604 4202 1604
Size: 2598 3692
BsrFI r'CCGG_y
Cuts at: 706 1022 1301 2568 2877 2962 3091 3212 4288
Size: 316 279 1267 309 85 129 121 1076
Cuts at: 4288 4469 4678 706
Size: 181 209 2318
Fragments arranged by size:
2318 1267 1076 316 309 279 209 181
129 121 85
BsrGI T'GTAC_A
Cuts at: 956 2267 956
Size: 1311 4979
BssHII G'CGCG_C
Cuts at: 2803 2803
Size: 6290
BssSI C'ACGA_G
Cuts at: 136 4561 4919 136
Size: 4425 358 1507
Fragments arranged by size:
4425 1507 358
BstAPI GCAn_nnn'nTGC
Cuts at: 387 1082 1180 2120 2951 3667 387
Size: 695 98 940 831 716 3010
Fragments arranged by size:
3010 940 831 716 695 98
BstBI TT'CG_AA
Cuts at: 4651 4651
Size: 6290
BstEII G'GTnAC_C
Cuts at: 1557 1557
Size: 6290
BstXI CCAn_nnnn'nTGG
Cuts at: 819 1911 2589 819
Size: 1092 678 4520
Fragments arranged by size:
4520 1092 678
BstYI r'GATC_y
Cuts at: 1 446 1397 2297 3315 3782 3805 4140 4386
Size: 445 951 900 1018 467 23 335 246
Cuts at: 4386 5398 5484 5496 5842 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1018 1012 951 900 467 449 445 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 751 1442 2054 2610 2623 2993 107
Size: 644 691 612 556 13 370 3404
Fragments arranged by size:
3404 691 644 612 556 370 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4240 4421 4584 4240
Size: 181 163 5946
Fragments arranged by size:
5946 181 163
BtsI GCAGTG_nn'
Cuts at: 373 669 834 1737 1784 2489 2709 2746 3533
Size: 296 165 903 47 705 220 37 787
Cuts at: 3533 373
Size: 3130
Fragments arranged by size:
3130 903 787 705 296 220 165 47
37
ClaI AT'CG_AT
Cuts at: 1522 1522
Size: 6290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 201 236 1688 1723 201
Size: 35 1452 35 4768
Fragments arranged by size:
4768 1452 35 35
DraI TTT'AAA
Cuts at: 3289 3289
Size: 6290
DraIII CAC_nnn'GTG
Cuts at: 138 1180 138
Size: 1042 5248
DrdI GACnn_nn'nnGTC
Cuts at: 1123 1888 3996 4854 1123
Size: 765 2108 858 2559
Fragments arranged by size:
2559 2108 858 765
EaeI y'GGCC_r
Cuts at: 191 209 287 704 971 1020 1194 1299 1901
Size: 18 78 417 267 49 174 105 602
Cuts at: 1901 1952 2360 2570 2620 3017 3210 3875 4049
Size: 51 408 210 50 397 193 665 174
Cuts at: 4049 4440 4467 5812 191
Size: 391 27 1345 669
Fragments arranged by size:
1345 669 665 602 417 408 397 391
267 210 193 174 174 105 78 51
50 49 27 18
EagI C'GGCC_G
Cuts at: 287 971 1020 2570 3017 3875 287
Size: 684 49 1550 447 858 2702
Fragments arranged by size:
2702 1550 858 684 447 49
EarI CTCTTCn'nnn_
Cuts at: 4313 4523 4313
Size: 210 6080
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4498 4808 4954 6100 4498
Size: 310 146 1146 4688
Fragments arranged by size:
4688 1146 310 146
EcoICRI GAG'CTC
Cuts at: 513 2037 3302 513
Size: 1524 1265 3501
Fragments arranged by size:
3501 1524 1265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 388 442 717 1015 1423 1690 1915 2323
Size: 3 54 275 298 408 267 225 408
Cuts at: 2323 2812 2884 4115 4547 5294 5562 385
Size: 489 72 1231 432 747 268 1113
Fragments arranged by size:
1231 1113 747 489 432 408 408 298
275 268 267 225 72 54 3
EcoNI CCTnn'n_nnAGG
Cuts at: 424 1111 2044 424
Size: 687 933 4670
Fragments arranged by size:
4670 933 687
EcoO109I rG'GnC_Cy
Cuts at: 532 1273 1530 1679 2172 2173 2193 2730 3139
Size: 741 257 149 493 1 20 537 409
Cuts at: 3139 3429 532
Size: 290 3393
Fragments arranged by size:
3393 741 537 493 409 290 257 149
20 1
EcoRI G'AATT_C
Cuts at: 3294 3294
Size: 6290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 552 781 801 1134 4309 552
Size: 229 20 333 3175 2533
Fragments arranged by size:
3175 2533 333 229 20
FseI GG_CCGG'CC
Cuts at: 3216 3216
Size: 6290
FspI TGC'GCA
Cuts at: 2124 4071 2124
Size: 1947 4343
HaeII r_GCGC'y
Cuts at: 2569 2652 2860 3724 3972 4994 5973 2569
Size: 83 208 864 248 1022 979 2886
Fragments arranged by size:
2886 1022 979 864 248 208 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 541 573 993 1025 1265 1297 2296 2328 3106
Size: 32 420 32 240 32 999 32 778
Cuts at: 3106 3138 3260 3292 4132 4162 4164 4194 541
Size: 32 122 32 840 30 2 30 2637
Fragments arranged by size:
2637 999 840 778 420 240 122 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2148 3329 2148
Size: 1181 5109
KasI G'GCGC_C
Cuts at: 2565 2648 3720 3968 2565
Size: 83 1072 248 4887
Fragments arranged by size:
4887 1072 248 83
KpnI G_GTAC'C
Cuts at: 3310 3310
Size: 6290
MfeI C'AATT_G
Cuts at: 497 497
Size: 6290
MluI A'CGCG_T
Cuts at: 5723 5723
Size: 6290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 683 4961 5145 683
Size: 4278 184 1828
Fragments arranged by size:
4278 1828 184
MscI TGG'CCA
Cuts at: 211 1903 1954 2362 4051 211
Size: 1692 51 408 1689 2450
Fragments arranged by size:
2450 1692 1689 408 51
MslI CAynn'nnrTG
Cuts at: 202 499 788 1756 2353 4279 4406 202
Size: 297 289 968 597 1926 127 2086
Fragments arranged by size:
2086 1926 968 597 297 289 127
MspA1I CmG'CkG
Cuts at: 566 899 1718 2386 2605 2790 3006 3119 3250
Size: 333 819 668 219 185 216 113 131
Cuts at: 3250 3401 3715 4075 5088 5333 566
Size: 151 314 360 1013 245 1523
Fragments arranged by size:
1523 1013 819 668 360 333 314 245
219 216 185 151 131 113
NaeI GCC'GGC
Cuts at: 708 1024 2570 3093 3214 4471 708
Size: 316 1546 523 121 1257 2527
Fragments arranged by size:
2527 1546 1257 523 316 121
NarI GG'CG_CC
Cuts at: 2566 2649 3721 3969 2566
Size: 83 1072 248 4887
Fragments arranged by size:
4887 1072 248 83
NcoI C'CATG_G
Cuts at: 107 1442 2993 107
Size: 1335 1551 3404
Fragments arranged by size:
3404 1551 1335
NgoMIV G'CCGG_C
Cuts at: 706 1022 2568 3091 3212 4469 706
Size: 316 1546 523 121 1257 2527
Fragments arranged by size:
2527 1546 1257 523 316 121
NspI r_CATG'y
Cuts at: 1428 3084 3343 3843 4750 1428
Size: 1656 259 500 907 2968
Fragments arranged by size:
2968 1656 907 500 259
PasI CC'CwG_GG
Cuts at: 234 2428 234
Size: 2194 4096
PciI A'CATG_T
Cuts at: 4746 4746
Size: 6290
PflMI CCAn_nnn'nTGG
Cuts at: 2557 2741 2557
Size: 184 6106
PfoI T'CCnGG_A
Cuts at: 1400 1871 1892 2132 2300 3278 1400
Size: 471 21 240 168 978 4412
Fragments arranged by size:
4412 978 471 240 168 21
PmeI GTTT'AAAC
Cuts at: 3289 3289
Size: 6290
PmlI CAC'GTG
Cuts at: 276 276
Size: 6290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5429 5461 5429
Size: 32 6258
PpuMI rG'GwC_Cy
Cuts at: 532 1273 1679 2730 3139 532
Size: 741 406 1051 409 3683
Fragments arranged by size:
3683 1051 741 409 406
PspOMI G'GGCC_C
Cuts at: 923 1772 2172 923
Size: 849 400 5041
Fragments arranged by size:
5041 849 400
PstI C_TGCA'G
Cuts at: 1145 2045 3011 3154 3337 4022 1145
Size: 900 966 143 183 685 3413
Fragments arranged by size:
3413 966 900 685 183 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6290
PvuII CAG'CTG
Cuts at: 2386 2790 3006 3119 3250 3715 4075 2386
Size: 404 216 113 131 465 360 4601
Fragments arranged by size:
4601 465 404 360 216 131 113
RsrII CG'GwC_CG
Cuts at: 4485 4485
Size: 6290
SacI G_AGCT'C
Cuts at: 515 2039 3304 515
Size: 1524 1265 3501
Fragments arranged by size:
3501 1524 1265
SalI G'TCGA_C
Cuts at: 2146 3327 2146
Size: 1181 5109
SanDI GG'GwC_CC
Cuts at: 532 1679 2730 3139 532
Size: 1147 1051 409 3683
Fragments arranged by size:
3683 1147 1051 409
SapI GCTCTTCn'nnn_
Cuts at: 4313 4523 4313
Size: 210 6080
SbfI CC_TGCA'GG
Cuts at: 3154 3337 3154
Size: 183 6107
ScaI AGT'ACT
Cuts at: 5807 5807
Size: 6290
SfcI C'TryA_G
Cuts at: 33 1141 2041 2079 3007 3150 3333 4018 5011
Size: 1108 900 38 928 143 183 685 993
Cuts at: 5011 5202 33
Size: 191 1121
Fragments arranged by size:
1121 1108 993 928 900 685 191 183
143 38
SfoI GGC'GCC
Cuts at: 2567 2650 3722 3970 2567
Size: 83 1072 248 4887
Fragments arranged by size:
4887 1072 248 83
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6290
SmaI CCC'GGG
Cuts at: 740 1615 2402 3241 3312 740
Size: 875 787 839 71 3718
Fragments arranged by size:
3718 875 839 787 71
SmlI C'TyrA_G
Cuts at: 1108 3445 4360 4852 5114 1108
Size: 2337 915 492 262 2284
Fragments arranged by size:
2337 2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6290
SphI G_CATG'C
Cuts at: 1428 3084 3343 3843 1428
Size: 1656 259 500 3875
Fragments arranged by size:
3875 1656 500 259
StuI AGG'CCT
Cuts at: 1593 1593
Size: 6290
StyI C'CwwG_G
Cuts at: 107 152 536 611 1442 2754 2993 3424 3778
Size: 45 384 75 831 1312 239 431 354
Cuts at: 3778 107
Size: 2619
Fragments arranged by size:
2619 1312 831 431 384 354 239 75
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 858 871 1086 2427 2557 3162 3873 4006 4465
Size: 13 215 1341 130 605 711 133 459
Cuts at: 4465 4674 858
Size: 209 2474
Fragments arranged by size:
2474 1341 711 605 459 215 209 133
130 13
TatI w'GTAC_w
Cuts at: 956 2267 5805 956
Size: 1311 3538 1441
Fragments arranged by size:
3538 1441 1311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 254 286 460 492 736 768 1049
Size: 32 59 32 174 32 244 32 281
Cuts at: 1049 1081 1431 1463 1688 1720 2608 2640 3158
Size: 32 350 32 225 32 888 32 518
Cuts at: 3158 3190 5807 5839 163
Size: 32 2617 32 614
Fragments arranged by size:
2617 888 614 518 350 281 244 225
174 59 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1555 4087 1555
Size: 2532 3758
XbaI T'CTAG_A
Cuts at: 3321 3321
Size: 6290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 1428 1596 1617 2164 2355 3785 201
Size: 1227 168 21 547 191 1430 2706
Fragments arranged by size:
2706 1430 1227 547 191 168 21
XmaI C'CCGG_G
Cuts at: 738 1613 2400 3239 3310 738
Size: 875 787 839 71 3718
Fragments arranged by size:
3718 875 839 787 71
ZraI GAC'GTC
Cuts at: 2721 2721
Size: 6290
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BbsI BcgI BfrBI BmtI
BsiWI BsmI BsmBI BstZ17I Bsu36I EcoRV FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI