Restriction map for
pF1KB7307
(Circular) (Six-base) MAPSORT of: pF1KB7307.seq Check: 3689 from: 1 to: 8231
pF1KB7307 8231 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1219 1219
Size: 8231
AccI GT'mk_AC
Cuts at: 2024 5269 2024
Size: 3245 4986
Acc65I G'GTAC_C
Cuts at: 3003 5247 3003
Size: 2244 5987
AclI AA'CG_TT
Cuts at: 8071 8071
Size: 8231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1143 1388 4661 6056 6488 7235 7503 568
Size: 575 245 3273 1395 432 747 268 1296
Fragments arranged by size:
3273 1395 1296 747 575 432 268 245
AflII C'TTAA_G
Cuts at: 777 777
Size: 8231
AflIII A'CryG_T
Cuts at: 1846 5583 6687 7664 1846
Size: 3737 1104 977 2413
Fragments arranged by size:
3737 2413 1104 977
AgeI A'CCGG_T
Cuts at: 6619 6619
Size: 8231
AhdI GACnn_n'nnGTC
Cuts at: 3220 3220
Size: 8231
AleI CACnn'nnGTG
Cuts at: 1160 1160
Size: 8231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4686 4718 4686
Size: 32 8199
AlwNI CAG_nnn'CTG
Cuts at: 517 707 4438 4831 5536 7103 517
Size: 190 3731 393 705 1567 1645
Fragments arranged by size:
3731 1645 1567 705 393 190
ApaLI G'TGCA_C
Cuts at: 4900 7001 4900
Size: 2101 6130
ApoI r'AATT_y
Cuts at: 190 269 520 569 655 1290 1677 2110 2435
Size: 79 251 49 86 635 387 433 325
Cuts at: 2435 2980 3257 3412 3547 3802 3982 4190 4451
Size: 545 277 155 135 255 180 208 261
Cuts at: 4451 4638 5235 7584 7840 190
Size: 187 597 2349 256 581
Fragments arranged by size:
2349 635 597 581 545 433 387 325
277 261 256 255 251 208 187 180
155 135 86 79 49
AseI AT'TA_AT
Cuts at: 20 1013 1082 20
Size: 993 69 7169
Fragments arranged by size:
7169 993 69
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8231
AvaI C'yCGr_G
Cuts at: 377 5251 377
Size: 4874 3357
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3764 3797 3764
Size: 33 8198
BamHI G'GATC_C
Cuts at: 5256 5256
Size: 8231
BanI G'GyrC_C
Cuts at: 1094 3003 3478 4821 5247 5661 5909 5944 1094
Size: 1909 475 1343 426 414 248 35 3381
Fragments arranged by size:
3381 1909 1343 475 426 414 248 35
BanII G_rGCy'C
Cuts at: 3090 3741 4627 5056 5245 6275 3090
Size: 651 886 429 189 1030 5046
Fragments arranged by size:
5046 1030 886 651 429 189
BbeI G_GCGC'C
Cuts at: 5665 5913 5665
Size: 248 7983
BbsI GAAGACnn'nnnn_
Cuts at: 440 1724 440
Size: 1284 6947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2137 2171 2137
Size: 34 8197
BciVI GTATCCnnnnn_n'
Cuts at: 158 2875 4434 6129 6890 8231 158
Size: 2717 1559 1695 761 1341 158
Fragments arranged by size:
2717 1695 1559 1341 761 158
BclI T'GATC_A
Cuts at: 1467 5751 1467
Size: 4284 3947
BfrBI ATG'CAT
Cuts at: 4753 4753
Size: 8231
BglI GCCn_nnn'nGGC
Cuts at: 3476 3476
Size: 8231
BglII A'GATC_T
Cuts at: 5746 7783 5746
Size: 2037 6194
BlpI GC'TnA_GC
Cuts at: 5343 5343
Size: 8231
Bme1580I G_kGCm'C
Cuts at: 1097 4904 5856 5949 7005 1097
Size: 3807 952 93 1056 2323
Fragments arranged by size:
3807 2323 1056 952 93
BmrI ACTGGGnnnn_n'
Cuts at: 2424 5443 5524 5859 8003 2424
Size: 3019 81 335 2144 2652
Fragments arranged by size:
3019 2652 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 6598 2957
Size: 3641 4590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 650 812 1557 2318 2738 3074 3179 3575
Size: 319 162 745 761 420 336 105 396
Cuts at: 3575 4688 4979 331
Size: 1113 291 3583
Fragments arranged by size:
3583 1113 761 745 420 396 336 319
291 162 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2293 3690 3761 3875 4970 5108 5407 6286 6778
Size: 1397 71 114 1095 138 299 879 492
Cuts at: 6778 7076 2293
Size: 298 3448
Fragments arranged by size:
3448 1397 1095 879 492 299 298 138
114 71
BsaI GGTCTCn'nnnn_
Cuts at: 2942 7935 2942
Size: 4993 3238
BsaAI yAC'GTr
Cuts at: 5584 5584
Size: 8231
BsaBI GATnn'nnATC
Cuts at: 6 689 1145 5745 7782 6
Size: 683 456 4600 2037 455
Fragments arranged by size:
4600 2037 683 456 455
BsaHI Gr'CG_yC
Cuts at: 5662 5910 6612 8108 5662
Size: 248 702 1496 5785
Fragments arranged by size:
5785 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5420 5941 6619 6893 7040 7451 5420
Size: 521 678 274 147 411 6200
Fragments arranged by size:
6200 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 4686 4716 304
Size: 30 4352 30 3819
Fragments arranged by size:
4352 3819 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 365 3013 365
Size: 2648 5583
BseYI C'CCAG_C
Cuts at: 2691 4405 4727 4879 5656 6414 6991 7487 2691
Size: 1714 322 152 777 758 577 496 3435
Fragments arranged by size:
3435 1714 777 758 577 496 322 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2697 3899 4583 2697
Size: 1202 684 6345
Fragments arranged by size:
6345 1202 684
BsiEI CG_ry'CG
Cuts at: 104 5773 5819 7027 104
Size: 5669 46 1208 1308
Fragments arranged by size:
5669 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 238 828 1157 3090 4904 5056 5245 6023 6213
Size: 590 329 1933 1814 152 189 778 190
Cuts at: 6213 7005 238
Size: 792 1464
Fragments arranged by size:
1933 1814 1464 792 778 590 329 190
189 152
BsmI GAATG_Cn'
Cuts at: 2777 4475 2777
Size: 1698 6533
BsmBI CGTCTCn'nnnn_
Cuts at: 4790 4790
Size: 8231
Bsp1286I G_dGCh'C
Cuts at: 238 828 1097 1157 3090 3741 4627 4904 5056
Size: 590 269 60 1933 651 886 277 152
Cuts at: 5056 5245 5856 5949 6023 6213 6275 7005 238
Size: 189 611 93 74 190 62 730 1464
Fragments arranged by size:
1933 1464 886 730 651 611 590 277
269 190 189 152 93 74 62 60
BspEI T'CCGG_A
Cuts at: 7451 7451
Size: 8231
BspHI T'CATG_A
Cuts at: 687 756 1017 7407 687
Size: 69 261 6390 1511
Fragments arranged by size:
6390 1511 261 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 4914 5100 5281 5495 5797 6178 1219
Size: 3695 186 181 214 302 381 3272
Fragments arranged by size:
3695 3272 381 302 214 186 181
BsrBI CCG'CTC
Cuts at: 50 144 6523 6577 8061 8226 50
Size: 94 6379 54 1484 165 55
Fragments arranged by size:
6379 1484 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 6143 6143
Size: 8231
BsrFI r'CCGG_y
Cuts at: 6229 6410 6619 6229
Size: 181 209 7841
Fragments arranged by size:
7841 209 181
BsrGI T'GTAC_A
Cuts at: 1984 3690 1984
Size: 1706 6525
BssSI C'ACGA_G
Cuts at: 6502 6860 6502
Size: 358 7873
BstAPI GCAn_nnn'nTGC
Cuts at: 4606 5608 4606
Size: 1002 7229
BstBI TT'CG_AA
Cuts at: 6592 6592
Size: 8231
BstXI CCAn_nnnn'nTGG
Cuts at: 400 4875 400
Size: 4475 3756
BstYI r'GATC_y
Cuts at: 1 134 664 2926 3832 4412 5256 5723 5746
Size: 133 530 2262 906 580 844 467 23
Cuts at: 5746 6081 6327 7339 7425 7437 7783 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2262 1012 906 844 580 530 467 449
346 335 246 133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 4629 4629
Size: 8231
BtgI C'CryG_G
Cuts at: 107 1838 1948 4166 4807 4874 5044 107
Size: 1731 110 2218 641 67 170 3294
Fragments arranged by size:
3294 2218 1731 641 170 110 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6181 6362 6525 6181
Size: 181 163 7887
Fragments arranged by size:
7887 181 163
BtsI GCAGTG_nn'
Cuts at: 1024 5474 1024
Size: 4450 3781
DraI TTT'AAA
Cuts at: 921 1755 2972 3987 5230 921
Size: 834 1217 1015 1243 3922
Fragments arranged by size:
3922 1243 1217 1015 834
DrdI GACnn_nn'nnGTC
Cuts at: 5937 6795 5937
Size: 858 7373
EaeI y'GGCC_r
Cuts at: 527 1951 2858 5816 5990 6381 6408 7753 527
Size: 1424 907 2958 174 391 27 1345 1005
Fragments arranged by size:
2958 1424 1345 1005 907 391 174 27
EagI C'GGCC_G
Cuts at: 5816 5816
Size: 8231
EarI CTCTTCn'nnn_
Cuts at: 476 1037 1865 2916 3986 4133 4272 5169 6254
Size: 561 828 1051 1070 147 139 897 1085
Cuts at: 6254 6464 476
Size: 210 2243
Fragments arranged by size:
2243 1085 1070 1051 897 828 561 210
147 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6439 6749 6895 8041 6439
Size: 310 146 1146 6629
Fragments arranged by size:
6629 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3088 5054 5243 3088
Size: 1966 189 6076
Fragments arranged by size:
6076 1966 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 568 650 812 1143 1388 1557 2318 2738
Size: 237 82 162 331 245 169 761 420
Cuts at: 2738 3074 3179 3575 4661 4688 4979 6056 6488
Size: 336 105 396 1086 27 291 1077 432
Cuts at: 6488 7235 7503 331
Size: 747 268 1059
Fragments arranged by size:
1086 1077 1059 761 747 432 420 396
336 331 291 268 245 237 169 162
105 82 27
EcoNI CCTnn'n_nnAGG
Cuts at: 632 1864 3584 4494 632
Size: 1232 1720 910 4369
Fragments arranged by size:
4369 1720 1232 910
EcoO109I rG'GnC_Cy
Cuts at: 5370 5370
Size: 8231
EcoRI G'AATT_C
Cuts at: 3802 5235 3802
Size: 1433 6798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2337 4618 6250 2337
Size: 2281 1632 4318
Fragments arranged by size:
4318 2281 1632
FspI TGC'GCA
Cuts at: 6012 6012
Size: 8231
HaeII r_GCGC'y
Cuts at: 5665 5913 6935 7914 5665
Size: 248 1022 979 5982
Fragments arranged by size:
5982 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 587 619 1432 1464 2182 2214 3206 3238 3467
Size: 32 813 32 718 32 992 32 229
Cuts at: 3467 3499 4006 4038 4132 4164 5041 5073 6073
Size: 32 507 32 94 32 877 32 1000
Cuts at: 6073 6103 6105 6135 587
Size: 30 2 30 2683
Fragments arranged by size:
2683 1000 992 877 813 718 507 229
94 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1440 3563 3662 5153 5270 1440
Size: 2123 99 1491 117 4401
Fragments arranged by size:
4401 2123 1491 117 99
HindIII A'AGCT_T
Cuts at: 2347 2347
Size: 8231
HpaI GTT'AAC
Cuts at: 3563 3563
Size: 8231
KasI G'GCGC_C
Cuts at: 5661 5909 5661
Size: 248 7983
KpnI G_GTAC'C
Cuts at: 3007 5251 3007
Size: 2244 5987
MluI A'CGCG_T
Cuts at: 7664 7664
Size: 8231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 1282 2156 3006 4446 5048 6902 7086 521
Size: 761 874 850 1440 602 1854 184 1666
Fragments arranged by size:
1854 1666 1440 874 850 761 602 184
MscI TGG'CCA
Cuts at: 529 1953 2860 5992 529
Size: 1424 907 3132 2768
Fragments arranged by size:
3132 2768 1424 907
MslI CAynn'nnrTG
Cuts at: 245 1160 4229 4873 6220 6347 245
Size: 915 3069 644 1347 127 2129
Fragments arranged by size:
3069 2129 1347 915 644 127
MspA1I CmG'CkG
Cuts at: 3688 4681 5342 5656 6016 7029 7274 3688
Size: 993 661 314 360 1013 245 4645
Fragments arranged by size:
4645 1013 993 661 360 314 245
NaeI GCC'GGC
Cuts at: 6412 6412
Size: 8231
NarI GG'CG_CC
Cuts at: 5662 5910 5662
Size: 248 7983
NcoI C'CATG_G
Cuts at: 107 1838 1948 4166 4807 4874 107
Size: 1731 110 2218 641 67 3464
Fragments arranged by size:
3464 2218 1731 641 110 67
NdeI CA'TA_TG
Cuts at: 1047 1047
Size: 8231
NgoMIV G'CCGG_C
Cuts at: 6410 6410
Size: 8231
NsiI A_TGCA'T
Cuts at: 4755 4755
Size: 8231
NspI r_CATG'y
Cuts at: 1481 1694 1850 5284 5784 6691 1481
Size: 213 156 3434 500 907 3021
Fragments arranged by size:
3434 3021 907 500 213 156
PciI A'CATG_T
Cuts at: 1846 6687 1846
Size: 4841 3390
PflMI CCAn_nnn'nTGG
Cuts at: 1069 4723 4831 1069
Size: 3654 108 4469
Fragments arranged by size:
4469 3654 108
PfoI T'CCnGG_A
Cuts at: 627 1534 2334 3552 627
Size: 907 800 1218 5306
Fragments arranged by size:
5306 1218 907 800
PmeI GTTT'AAAC
Cuts at: 5230 5230
Size: 8231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 773 805 3118 3150 4686 4718 7370 7402 773
Size: 32 2313 32 1536 32 2652 32 1602
Fragments arranged by size:
2652 2313 1602 1536 32 32 32 32
PspXI vC'TCGA_Gb
Cuts at: 377 377
Size: 8231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1035 1067 1035
Size: 32 8199
PstI C_TGCA'G
Cuts at: 2490 3054 4925 5278 5963 2490
Size: 564 1871 353 685 4758
Fragments arranged by size:
4758 1871 685 564 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8231
PvuII CAG'CTG
Cuts at: 3688 4681 5656 6016 3688
Size: 993 975 360 5903
Fragments arranged by size:
5903 993 975 360
RsrII CG'GwC_CG
Cuts at: 6426 6426
Size: 8231
SacI G_AGCT'C
Cuts at: 3090 5056 5245 3090
Size: 1966 189 6076
Fragments arranged by size:
6076 1966 189
SalI G'TCGA_C
Cuts at: 5268 5268
Size: 8231
SapI GCTCTTCn'nnn_
Cuts at: 4133 6254 6464 4133
Size: 2121 210 5900
Fragments arranged by size:
5900 2121 210
SbfI CC_TGCA'GG
Cuts at: 5278 5278
Size: 8231
ScaI AGT'ACT
Cuts at: 3076 3228 7748 3076
Size: 152 4520 3559
Fragments arranged by size:
4520 3559 152
SexAI A'CCwGG_T
Cuts at: 3579 3579
Size: 8231
SfcI C'TryA_G
Cuts at: 33 359 476 2486 3050 4921 5274 5959 6952
Size: 326 117 2010 564 1871 353 685 993
Cuts at: 6952 7143 33
Size: 191 1121
Fragments arranged by size:
2010 1871 1121 993 685 564 353 326
191 117
SfoI GGC'GCC
Cuts at: 5663 5911 5663
Size: 248 7983
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8231
SmaI CCC'GGG
Cuts at: 5253 5253
Size: 8231
SmlI C'TyrA_G
Cuts at: 377 777 2272 3705 3776 3890 4985 5123 5386
Size: 400 1495 1433 71 114 1095 138 263
Cuts at: 5386 6301 6793 7055 377
Size: 915 492 262 1553
Fragments arranged by size:
1553 1495 1433 1095 915 492 400 263
262 138 114 71
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8231
SphI G_CATG'C
Cuts at: 1694 5284 5784 1694
Size: 3590 500 4141
Fragments arranged by size:
4141 3590 500
SspI AAT'ATT
Cuts at: 1738 2827 1738
Size: 1089 7142
StyI C'CwwG_G
Cuts at: 107 1838 1948 2868 3611 4166 4807 4874 5365
Size: 1731 110 920 743 555 641 67 491
Cuts at: 5365 5719 107
Size: 354 2619
Fragments arranged by size:
2619 1731 920 743 641 555 491 354
110 67
SwaI ATTT'AAAT
Cuts at: 921 921
Size: 8231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 5814 5947 6406 6615 5814
Size: 133 459 209 7430
Fragments arranged by size:
7430 459 209 133
TatI w'GTAC_w
Cuts at: 1594 1656 1984 2427 2837 3074 3226 3519 3690
Size: 62 328 443 410 237 152 293 171
Cuts at: 3690 4104 7746 1594
Size: 414 3642 2079
Fragments arranged by size:
3642 2079 443 414 410 328 293 237
171 152 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1589 1621 4375 4407 4805 4837 7748 7780 1589
Size: 32 2754 32 398 32 2911 32 2040
Fragments arranged by size:
2911 2754 2040 398 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 707 6028 707
Size: 5321 2910
XbaI T'CTAG_A
Cuts at: 2553 5262 2553
Size: 2709 5522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 625 4724 5726 625
Size: 4099 1002 3130
Fragments arranged by size:
4099 3130 1002
XhoI C'TCGA_G
Cuts at: 377 377
Size: 8231
XmaI C'CCGG_G
Cuts at: 5251 5251
Size: 8231
XmnI GAAnn'nnTTC
Cuts at: 346 470 788 1646 3062 3185 3548 4455 4840
Size: 124 318 858 1416 123 363 907 385
Cuts at: 4840 346
Size: 3737
Fragments arranged by size:
3737 1416 907 858 385 363 318 124
123
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AlfI ApaI AscI AvrII BbvCI BmgBI
BmtI Bpu10I BsiWI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI MfeI NheI NotI NruI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI ZraI