Restriction map for
pF1KB7306
(Circular) (Six-base) MAPSORT of: pF1KB7306.seq Check: 909 from: 1 to: 6305
pF1KB7306 6305 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1312 1522 1912 1312
Size: 210 390 5705
Fragments arranged by size:
5705 390 210
AccI GT'mk_AC
Cuts at: 3343 3343
Size: 6305
Acc65I G'GTAC_C
Cuts at: 3321 3321
Size: 6305
AclI AA'CG_TT
Cuts at: 6145 6145
Size: 6305
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1169 4130 4562 4915 5577 977
Size: 192 2961 432 353 662 1705
Fragments arranged by size:
2961 1705 662 432 353 192
AflIII A'CryG_T
Cuts at: 2425 2957 3657 5457 5738 2425
Size: 532 700 1800 281 2992
Fragments arranged by size:
2992 1800 700 532 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1221 1221
Size: 6305
AlwNI CAG_nnn'CTG
Cuts at: 1803 3610 5048 1803
Size: 1807 1438 3060
Fragments arranged by size:
3060 1807 1438
ApaI G_GGCC'C
Cuts at: 1279 1489 1279
Size: 210 6095
ApaLI G'TGCA_C
Cuts at: 497 2917 5143 497
Size: 2420 2226 1659
Fragments arranged by size:
2420 2226 1659
ApoI r'AATT_y
Cuts at: 202 253 752 896 942 1600 2008 2659 2860
Size: 51 499 144 46 658 408 651 201
Cuts at: 2860 3309 5658 5914 202
Size: 449 2349 256 593
Fragments arranged by size:
2349 658 651 593 499 449 408 256
201 144 51 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 6305
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6305
AvaI C'yCGr_G
Cuts at: 2273 2474 3325 2273
Size: 201 851 5253
Fragments arranged by size:
5253 851 201
BamHI G'GATC_C
Cuts at: 3330 3330
Size: 6305
BanI G'GyrC_C
Cuts at: 3321 3735 3983 4018 3321
Size: 414 248 35 5608
Fragments arranged by size:
5608 414 248 35
BanII G_rGCy'C
Cuts at: 366 1279 1489 3319 4349 366
Size: 913 210 1830 1030 2322
Fragments arranged by size:
2322 1830 1030 913 210
BbeI G_GCGC'C
Cuts at: 3739 3987 3739
Size: 248 6057
BbsI GAAGACnn'nnnn_
Cuts at: 627 676 1314 2112 2265 2594 3050 627
Size: 49 638 798 153 329 456 3882
Fragments arranged by size:
3882 798 638 456 329 153 49
BciVI GTATCCnnnnn_n'
Cuts at: 2415 4203 5259 6305 2415
Size: 1788 1056 1046 2415
Fragments arranged by size:
2415 1788 1056 1046
BclI T'GATC_A
Cuts at: 3825 3825
Size: 6305
BfrBI ATG'CAT
Cuts at: 867 1083 1503 1680 867
Size: 216 420 177 5492
Fragments arranged by size:
5492 420 216 177
BglI GCCn_nnn'nGGC
Cuts at: 857 1670 2078 2729 857
Size: 813 408 651 4433
Fragments arranged by size:
4433 813 651 408
BglII A'GATC_T
Cuts at: 3820 5857 3820
Size: 2037 4268
BlpI GC'TnA_GC
Cuts at: 701 3417 701
Size: 2716 3589
Bme1580I G_kGCm'C
Cuts at: 501 885 1279 1489 2921 3930 4023 5147 501
Size: 384 394 210 1432 1009 93 1124 1659
Fragments arranged by size:
1659 1432 1124 1009 394 384 210 93
BmrI ACTGGGnnnn_n'
Cuts at: 2908 3517 3598 3933 6077 2908
Size: 609 81 335 2144 3136
Fragments arranged by size:
3136 2144 609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4672 4672
Size: 6305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2370 2370
Size: 6305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3481 4360 5074 5372 3020
Size: 461 879 714 298 3953
Fragments arranged by size:
3953 879 714 461 298
BsaI GGTCTCn'nnnn_
Cuts at: 6009 6009
Size: 6305
BsaAI yAC'GTr
Cuts at: 302 2293 3658 302
Size: 1991 1365 2949
Fragments arranged by size:
2949 1991 1365
BsaBI GATnn'nnATC
Cuts at: 6 2409 2740 3819 5856 6
Size: 2403 331 1079 2037 455
Fragments arranged by size:
2403 2037 1079 455 331
BsaHI Gr'CG_yC
Cuts at: 3736 3984 4686 6182 3736
Size: 248 702 1496 3859
Fragments arranged by size:
3859 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3494 4015 4693 5104 5251 5525 3494
Size: 521 678 411 147 274 4274
Fragments arranged by size:
4274 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1291 1321 1501 1531 1891 1921 1291
Size: 30 180 30 360 30 5675
Fragments arranged by size:
5675 360 180 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1423 2219 3262 1423
Size: 796 1043 4466
Fragments arranged by size:
4466 1043 796
BseYI C'CCAG_C
Cuts at: 3730 4488 5153 5561 3730
Size: 758 665 408 4474
Fragments arranged by size:
4474 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1827 2028 2454 2679 2874 2982 1827
Size: 201 426 225 195 108 5150
Fragments arranged by size:
5150 426 225 201 195 108
BsiEI CG_ry'CG
Cuts at: 104 3847 3893 5123 104
Size: 3743 46 1230 1286
Fragments arranged by size:
3743 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 366 501 2921 3319 4097 4287 5147 366
Size: 135 2420 398 778 190 860 1524
Fragments arranged by size:
2420 1524 860 778 398 190 135
BsmI GAATG_Cn'
Cuts at: 519 519
Size: 6305
Bsp1286I G_dGCh'C
Cuts at: 366 501 885 1279 1489 2921 3319 3930 4023
Size: 135 384 394 210 1432 398 611 93
Cuts at: 4023 4097 4287 4349 5147 366
Size: 74 190 62 798 1524
Fragments arranged by size:
1524 1432 798 611 398 394 384 210
190 135 93 74 62
BspHI T'CATG_A
Cuts at: 3192 4737 3192
Size: 1545 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1312 1522 1912 3355 3569 3871 4252 1312
Size: 210 390 1443 214 302 381 3365
Fragments arranged by size:
3365 1443 390 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 4597 4651 6135 6300 50
Size: 4547 54 1484 165 55
Fragments arranged by size:
4547 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 698 2640 4217 698
Size: 1942 1577 2786
Fragments arranged by size:
2786 1942 1577
BsrFI r'CCGG_y
Cuts at: 4303 4484 4303
Size: 181 6124
BsrGI T'GTAC_A
Cuts at: 823 823
Size: 6305
BssSI C'ACGA_G
Cuts at: 1154 4576 5284 1154
Size: 3422 708 2175
Fragments arranged by size:
3422 2175 708
BstAPI GCAn_nnn'nTGC
Cuts at: 505 697 1304 1514 1904 3682 505
Size: 192 607 210 390 1778 3128
Fragments arranged by size:
3128 1778 607 390 210 192
BstBI TT'CG_AA
Cuts at: 2664 2865 4666 2664
Size: 201 1801 4303
Fragments arranged by size:
4303 1801 201
BstYI r'GATC_y
Cuts at: 1 2646 3330 3797 3820 4155 4401 4707 4719
Size: 2645 684 467 23 335 246 306 12
Cuts at: 4719 4805 5857 1
Size: 86 1052 449
Fragments arranged by size:
2645 1052 684 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 846 1659 1893 2088 846
Size: 813 234 195 5063
Fragments arranged by size:
5063 813 234 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2018 4255 4436 4599 2018
Size: 2237 181 163 3724
Fragments arranged by size:
3724 2237 181 163
BtsI GCAGTG_nn'
Cuts at: 597 1215 1602 1815 2862 3548 597
Size: 618 387 213 1047 686 3354
Fragments arranged by size:
3354 1047 686 618 387 213
DraI TTT'AAA
Cuts at: 408 713 3137 3304 408
Size: 305 2424 167 3409
Fragments arranged by size:
3409 2424 305 167
DraIII CAC_nnn'GTG
Cuts at: 505 505
Size: 6305
DrdI GACnn_nn'nnGTC
Cuts at: 4011 5355 4011
Size: 1344 4961
EaeI y'GGCC_r
Cuts at: 3159 3890 4064 4455 4482 5827 3159
Size: 731 174 391 27 1345 3637
Fragments arranged by size:
3637 1345 731 391 174 27
EagI C'GGCC_G
Cuts at: 3890 3890
Size: 6305
EarI CTCTTCn'nnn_
Cuts at: 334 1724 2150 2804 4328 4538 334
Size: 1390 426 654 1524 210 2101
Fragments arranged by size:
2101 1524 1390 654 426 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4513 5255 5401 6115 4513
Size: 742 146 714 4703
Fragments arranged by size:
4703 742 714 146
EcoICRI GAG'CTC
Cuts at: 364 3317 364
Size: 2953 3352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 977 1169 2370 4130 4562 4915 5577 977
Size: 192 1201 1760 432 353 662 1705
Fragments arranged by size:
1760 1705 1201 662 432 353 192
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2717
Size: 6305
EcoO109I rG'GnC_Cy
Cuts at: 527 1275 1485 3444 527
Size: 748 210 1959 3388
Fragments arranged by size:
3388 1959 748 210
EcoRI G'AATT_C
Cuts at: 752 3309 752
Size: 2557 3748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2136 4324 2136
Size: 2188 4117
FspI TGC'GCA
Cuts at: 4086 4086
Size: 6305
HaeII r_GCGC'y
Cuts at: 3739 3987 5217 5988 3739
Size: 248 1230 771 4056
Fragments arranged by size:
4056 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 1958 1990 2293 2325 3102 3134 4147
Size: 32 1371 32 303 32 777 32 1013
Cuts at: 4147 4177 4179 4209 555
Size: 30 2 30 2651
Fragments arranged by size:
2651 1371 1013 777 303 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3344 3344
Size: 6305
HindIII A'AGCT_T
Cuts at: 1222 2686 2984 3001 1222
Size: 1464 298 17 4526
Fragments arranged by size:
4526 1464 298 17
KasI G'GCGC_C
Cuts at: 3735 3983 3735
Size: 248 6057
KpnI G_GTAC'C
Cuts at: 3325 3325
Size: 6305
MluI A'CGCG_T
Cuts at: 5738 5738
Size: 6305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5064 5248 5064
Size: 184 6121
MscI TGG'CCA
Cuts at: 3161 4066 3161
Size: 905 5400
MslI CAynn'nnrTG
Cuts at: 1632 4294 4421 1632
Size: 2662 127 3516
Fragments arranged by size:
3516 2662 127
MspA1I CmG'CkG
Cuts at: 2267 2563 3416 3730 4090 4874 5119 2267
Size: 296 853 314 360 784 245 3453
Fragments arranged by size:
3453 853 784 360 314 296 245
NaeI GCC'GGC
Cuts at: 4486 4486
Size: 6305
NarI GG'CG_CC
Cuts at: 3736 3984 3736
Size: 248 6057
NcoI C'CATG_G
Cuts at: 2088 2088
Size: 6305
NdeI CA'TA_TG
Cuts at: 483 1194 2727 483
Size: 711 1533 4061
Fragments arranged by size:
4061 1533 711
NgoMIV G'CCGG_C
Cuts at: 4484 4484
Size: 6305
NsiI A_TGCA'T
Cuts at: 869 1085 1505 1682 869
Size: 216 420 177 5492
Fragments arranged by size:
5492 420 216 177
NspI r_CATG'y
Cuts at: 2003 2429 2673 2961 3186 3285 3358 3858 5461
Size: 426 244 288 225 99 73 500 1603
Cuts at: 5461 2003
Size: 2847
Fragments arranged by size:
2847 1603 500 426 288 244 225 99
73
PciI A'CATG_T
Cuts at: 2425 2957 5457 2425
Size: 532 2500 3273
Fragments arranged by size:
3273 2500 532
PflMI CCAn_nnn'nTGG
Cuts at: 847 847
Size: 6305
PfoI T'CCnGG_A
Cuts at: 1435 1435
Size: 6305
PmeI GTTT'AAAC
Cuts at: 3304 3304
Size: 6305
PmlI CAC'GTG
Cuts at: 302 302
Size: 6305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1778 1810 4751 4783 1778
Size: 32 2941 32 3300
Fragments arranged by size:
3300 2941 32 32
PpuMI rG'GwC_Cy
Cuts at: 527 527
Size: 6305
PspOMI G'GGCC_C
Cuts at: 1275 1485 1275
Size: 210 6095
PstI C_TGCA'G
Cuts at: 1000 1597 2181 3352 4037 1000
Size: 597 584 1171 685 3268
Fragments arranged by size:
3268 1171 685 597 584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6305
PvuII CAG'CTG
Cuts at: 2267 2563 3730 4090 2267
Size: 296 1167 360 4482
Fragments arranged by size:
4482 1167 360 296
RsrII CG'GwC_CG
Cuts at: 4500 4500
Size: 6305
SacI G_AGCT'C
Cuts at: 366 3319 366
Size: 2953 3352
SalI G'TCGA_C
Cuts at: 3342 3342
Size: 6305
SanDI GG'GwC_CC
Cuts at: 527 527
Size: 6305
SapI GCTCTTCn'nnn_
Cuts at: 4328 4538 4328
Size: 210 6095
SbfI CC_TGCA'GG
Cuts at: 3352 3352
Size: 6305
ScaI AGT'ACT
Cuts at: 5822 5822
Size: 6305
SfcI C'TryA_G
Cuts at: 33 996 1593 2177 3348 4033 5001 5192 33
Size: 963 597 584 1171 685 968 191 1146
Fragments arranged by size:
1171 1146 968 963 685 597 584 191
SfoI GGC'GCC
Cuts at: 3737 3985 3737
Size: 248 6057
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6305
SmaI CCC'GGG
Cuts at: 3327 3327
Size: 6305
SmlI C'TyrA_G
Cuts at: 2474 3035 3460 4375 5089 5351 2474
Size: 561 425 915 714 262 3428
Fragments arranged by size:
3428 915 714 561 425 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6305
SphI G_CATG'C
Cuts at: 2673 3186 3285 3358 3858 2673
Size: 513 99 73 500 5120
Fragments arranged by size:
5120 513 500 99 73
StuI AGG'CCT
Cuts at: 1531 1531
Size: 6305
StyI C'CwwG_G
Cuts at: 140 368 2088 3439 3793 140
Size: 228 1720 1351 354 2652
Fragments arranged by size:
2652 1720 1351 354 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3888 4021 4480 4689 3888
Size: 133 459 209 5504
Fragments arranged by size:
5504 459 209 133
TatI w'GTAC_w
Cuts at: 823 1393 2269 2527 3232 5820 823
Size: 570 876 258 705 2588 1308
Fragments arranged by size:
2588 1308 876 705 570 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5822 5854 5822
Size: 32 6273
Tth111I GACn'n_nGTC
Cuts at: 2488 4102 2488
Size: 1614 4691
XbaI T'CTAG_A
Cuts at: 1878 3336 1878
Size: 1458 4847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1279 1489 1618 2452 3800 1279
Size: 210 129 834 1348 3784
Fragments arranged by size:
3784 1348 834 210 129
XhoI C'TCGA_G
Cuts at: 2474 2474
Size: 6305
XmaI C'CCGG_G
Cuts at: 3325 3325
Size: 6305
XmnI GAAnn'nnTTC
Cuts at: 1793 1793
Size: 6305
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI
NruI PacI PasI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI