Restriction map for
pF1KB7305
(Circular) (Six-base) MAPSORT of: pF1KB7305.seq Check: 8080 from: 1 to: 5543
pF1KB7305 5543 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 2581 2581
Size: 5543
Acc65I G'GTAC_C
Cuts at: 2559 2559
Size: 5543
AclI AA'CG_TT
Cuts at: 5383 5383
Size: 5543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1612 3368 3800 4547 4815 1171
Size: 441 1756 432 747 268 1899
Fragments arranged by size:
1899 1756 747 441 432 268
AflIII A'CryG_T
Cuts at: 2895 3999 4976 2895
Size: 1104 977 3462
Fragments arranged by size:
3462 1104 977
AgeI A'CCGG_T
Cuts at: 3931 3931
Size: 5543
AhdI GACnn_n'nnGTC
Cuts at: 1699 1699
Size: 5543
AlwNI CAG_nnn'CTG
Cuts at: 2848 4415 2848
Size: 1567 3976
ApaLI G'TGCA_C
Cuts at: 641 4313 641
Size: 3672 1871
ApoI r'AATT_y
Cuts at: 2547 4896 5152 2547
Size: 2349 256 2938
Fragments arranged by size:
2938 2349 256
AseI AT'TA_AT
Cuts at: 20 916 20
Size: 896 4647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
AvaI C'yCGr_G
Cuts at: 2505 2563 2505
Size: 58 5485
BamHI G'GATC_C
Cuts at: 2568 2568
Size: 5543
BanI G'GyrC_C
Cuts at: 2231 2559 2973 3221 3256 2231
Size: 328 414 248 35 4518
Fragments arranged by size:
4518 414 328 248 35
BanII G_rGCy'C
Cuts at: 193 1022 2228 2557 3587 193
Size: 829 1206 329 1030 2149
Fragments arranged by size:
2149 1206 1030 829 329
BbeI G_GCGC'C
Cuts at: 2977 3225 2977
Size: 248 5295
BbsI GAAGACnn'nnnn_
Cuts at: 2456 2456
Size: 5543
BciVI GTATCCnnnnn_n'
Cuts at: 590 3441 4202 5543 590
Size: 2851 761 1341 590
Fragments arranged by size:
2851 1341 761 590
BclI T'GATC_A
Cuts at: 3063 3063
Size: 5543
BfrBI ATG'CAT
Cuts at: 1066 1066
Size: 5543
BglI GCCn_nnn'nGGC
Cuts at: 2240 2249 2240
Size: 9 5534
BglII A'GATC_T
Cuts at: 418 3058 5095 418
Size: 2640 2037 866
Fragments arranged by size:
2640 2037 866
BlpI GC'TnA_GC
Cuts at: 187 2114 2655 187
Size: 1927 541 3075
Fragments arranged by size:
3075 1927 541
Bme1580I G_kGCm'C
Cuts at: 645 3168 3261 4317 645
Size: 2523 93 1056 1871
Fragments arranged by size:
2523 1871 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1757 2755 2836 3171 5315 1757
Size: 998 81 335 2144 1985
Fragments arranged by size:
2144 1985 998 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 212 3910 212
Size: 3698 1845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2482 2482
Size: 5543
Bpu10I CC'TnA_GC
Cuts at: 2227 2227
Size: 5543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2719 3598 4090 4388 2719
Size: 879 492 298 3874
Fragments arranged by size:
3874 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2458 5247 2458
Size: 2789 2754
BsaAI yAC'GTr
Cuts at: 2896 2896
Size: 5543
BsaBI GATnn'nnATC
Cuts at: 6 3057 5094 6
Size: 3051 2037 455
Fragments arranged by size:
3051 2037 455
BsaHI Gr'CG_yC
Cuts at: 2974 3222 3924 5420 2974
Size: 248 702 1496 3097
Fragments arranged by size:
3097 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2732 3253 3931 4205 4352 4763 2732
Size: 521 678 274 147 411 3512
Fragments arranged by size:
3512 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2968 3726 4303 4799 2968
Size: 758 577 496 3712
Fragments arranged by size:
3712 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1949 1972 1949
Size: 23 5520
BsiEI CG_ry'CG
Cuts at: 104 3085 3131 4339 104
Size: 2981 46 1208 1308
Fragments arranged by size:
2981 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 193 645 1022 2228 2557 3335 3525 4317 193
Size: 452 377 1206 329 778 190 792 1419
Fragments arranged by size:
1419 1206 792 778 452 377 329 190
BsmI GAATG_Cn'
Cuts at: 2032 2032
Size: 5543
Bsp1286I G_dGCh'C
Cuts at: 193 645 1022 2228 2557 3168 3261 3335 3525
Size: 452 377 1206 329 611 93 74 190
Cuts at: 3525 3587 4317 193
Size: 62 730 1419
Fragments arranged by size:
1419 1206 730 611 452 377 329 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4763 4763
Size: 5543
BspHI T'CATG_A
Cuts at: 2220 4719 2220
Size: 2499 3044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2593 2807 3109 3490 2593
Size: 214 302 381 4646
Fragments arranged by size:
4646 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2499 3835 3889 5373 5538 50
Size: 2449 1336 54 1484 165 55
Fragments arranged by size:
2449 1484 1336 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 1640 2108 3455 722
Size: 918 468 1347 2810
Fragments arranged by size:
2810 1347 918 468
BsrFI r'CCGG_y
Cuts at: 3541 3722 3931 3541
Size: 181 209 5153
Fragments arranged by size:
5153 209 181
BsrGI T'GTAC_A
Cuts at: 1026 1026
Size: 5543
BssSI C'ACGA_G
Cuts at: 3814 4172 3814
Size: 358 5185
BstAPI GCAn_nnn'nTGC
Cuts at: 2920 2920
Size: 5543
BstBI TT'CG_AA
Cuts at: 1911 3904 1911
Size: 1993 3550
BstEII G'GTnAC_C
Cuts at: 111 111
Size: 5543
BstXI CCAn_nnnn'nTGG
Cuts at: 252 2185 252
Size: 1933 3610
BstYI r'GATC_y
Cuts at: 1 418 2568 3035 3058 3393 3639 4651 4737
Size: 417 2150 467 23 335 246 1012 86
Cuts at: 4737 4749 5095 1
Size: 12 346 449
Fragments arranged by size:
2150 1012 467 449 417 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3493 3674 3837 3493
Size: 181 163 5199
Fragments arranged by size:
5199 181 163
BtsI GCAGTG_nn'
Cuts at: 645 2426 2786 645
Size: 1781 360 3402
Fragments arranged by size:
3402 1781 360
DraI TTT'AAA
Cuts at: 948 1424 2542 948
Size: 476 1118 3949
Fragments arranged by size:
3949 1118 476
DrdI GACnn_nn'nnGTC
Cuts at: 3249 4107 3249
Size: 858 4685
EaeI y'GGCC_r
Cuts at: 242 3128 3302 3693 3720 5065 242
Size: 2886 174 391 27 1345 720
Fragments arranged by size:
2886 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 3128 3128
Size: 5543
EarI CTCTTCn'nnn_
Cuts at: 456 1373 1579 1908 2508 3566 3776 456
Size: 917 206 329 600 1058 210 2223
Fragments arranged by size:
2223 1058 917 600 329 210 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3751 4061 4207 5353 3751
Size: 310 146 1146 3941
Fragments arranged by size:
3941 1146 310 146
EcoICRI GAG'CTC
Cuts at: 191 1020 2226 2555 191
Size: 829 1206 329 3179
Fragments arranged by size:
3179 1206 829 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1612 2482 3368 3800 4547 4815 1171
Size: 441 870 886 432 747 268 1899
Fragments arranged by size:
1899 886 870 747 441 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 775 775
Size: 5543
EcoO109I rG'GnC_Cy
Cuts at: 2682 2682
Size: 5543
EcoRI G'AATT_C
Cuts at: 2547 2547
Size: 5543
EcoRV GAT'ATC
Cuts at: 1134 1134
Size: 5543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 787 1999 2161 2173 3562 787
Size: 1212 162 12 1389 2768
Fragments arranged by size:
2768 1389 1212 162 12
FspI TGC'GCA
Cuts at: 3324 3324
Size: 5543
HaeII r_GCGC'y
Cuts at: 1731 2977 3225 4247 5226 1731
Size: 1246 248 1022 979 2048
Fragments arranged by size:
2048 1246 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 394 426 588 620 1685 1717 2299
Size: 32 131 32 162 32 1065 32 582
Cuts at: 2299 2331 3385 3415 3417 3447 231
Size: 32 1054 30 2 30 2327
Fragments arranged by size:
2327 1065 1054 582 162 131 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2292 2582 2292
Size: 290 5253
HindIII A'AGCT_T
Cuts at: 893 1336 2143 893
Size: 443 807 4293
Fragments arranged by size:
4293 807 443
KasI G'GCGC_C
Cuts at: 2973 3221 2973
Size: 248 5295
KpnI G_GTAC'C
Cuts at: 2563 2563
Size: 5543
MfeI C'AATT_G
Cuts at: 1112 1112
Size: 5543
MluI A'CGCG_T
Cuts at: 4976 4976
Size: 5543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 840 1490 4214 4398 840
Size: 650 2724 184 1985
Fragments arranged by size:
2724 1985 650 184
MscI TGG'CCA
Cuts at: 244 3304 244
Size: 3060 2483
MslI CAynn'nnrTG
Cuts at: 1645 3532 3659 1645
Size: 1887 127 3529
Fragments arranged by size:
3529 1887 127
MspA1I CmG'CkG
Cuts at: 2654 2968 3328 4341 4586 2654
Size: 314 360 1013 245 3611
Fragments arranged by size:
3611 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3724 3724
Size: 5543
NarI GG'CG_CC
Cuts at: 2974 3222 2974
Size: 248 5295
NcoI C'CATG_G
Cuts at: 107 107
Size: 5543
NgoMIV G'CCGG_C
Cuts at: 3722 3722
Size: 5543
NsiI A_TGCA'T
Cuts at: 1068 1068
Size: 5543
NspI r_CATG'y
Cuts at: 2596 3096 4003 2596
Size: 500 907 4136
Fragments arranged by size:
4136 907 500
PciI A'CATG_T
Cuts at: 3999 3999
Size: 5543
PmeI GTTT'AAAC
Cuts at: 2542 2542
Size: 5543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 4682
Size: 32 5511
PstI C_TGCA'G
Cuts at: 2590 3275 2590
Size: 685 4858
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5543
PvuII CAG'CTG
Cuts at: 2968 3328 2968
Size: 360 5183
RsrII CG'GwC_CG
Cuts at: 3738 3738
Size: 5543
SacI G_AGCT'C
Cuts at: 193 1022 2228 2557 193
Size: 829 1206 329 3179
Fragments arranged by size:
3179 1206 829 329
SalI G'TCGA_C
Cuts at: 2580 2580
Size: 5543
SapI GCTCTTCn'nnn_
Cuts at: 456 1373 1908 3566 3776 456
Size: 917 535 1658 210 2223
Fragments arranged by size:
2223 1658 917 535 210
SbfI CC_TGCA'GG
Cuts at: 2590 2590
Size: 5543
ScaI AGT'ACT
Cuts at: 5060 5060
Size: 5543
SfcI C'TryA_G
Cuts at: 33 1489 1939 2586 3271 4264 4455 33
Size: 1456 450 647 685 993 191 1121
Fragments arranged by size:
1456 1121 993 685 647 450 191
SfoI GGC'GCC
Cuts at: 2975 3223 2975
Size: 248 5295
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
SmaI CCC'GGG
Cuts at: 2565 2565
Size: 5543
SmlI C'TyrA_G
Cuts at: 2698 3613 4105 4367 2698
Size: 915 492 262 3874
Fragments arranged by size:
3874 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5543
SphI G_CATG'C
Cuts at: 2596 3096 2596
Size: 500 5043
SspI AAT'ATT
Cuts at: 1617 1617
Size: 5543
StyI C'CwwG_G
Cuts at: 107 771 2677 3031 107
Size: 664 1906 354 2619
Fragments arranged by size:
2619 1906 664 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1782 3126 3259 3718 3927 195
Size: 1587 1344 133 459 209 1811
Fragments arranged by size:
1811 1587 1344 459 209 133
TatI w'GTAC_w
Cuts at: 1026 5058 1026
Size: 4032 1511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5060 5092 5060
Size: 32 5511
Tth111I GACn'n_nGTC
Cuts at: 3340 3340
Size: 5543
XbaI T'CTAG_A
Cuts at: 2574 2574
Size: 5543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3038
Size: 5543
XmaI C'CCGG_G
Cuts at: 2563 2563
Size: 5543
XmnI GAAnn'nnTTC
Cuts at: 584 584
Size: 5543
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI BsaXI
BseRI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HpaI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI