Restriction map for
pF1KB7303
(Circular) (Six-base) MAPSORT of: pF1KB7303.seq Check: 7063 from: 1 to: 6977
pF1KB7303 6977 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 888 1853 2600 705
Size: 183 965 747 5082
Fragments arranged by size:
5082 965 747 183
AatII G_ACGT'C
Cuts at: 170 3808 170
Size: 3638 3339
AccI GT'mk_AC
Cuts at: 4015 4015
Size: 6977
Acc65I G'GTAC_C
Cuts at: 154 3569 3993 154
Size: 3415 424 3138
Fragments arranged by size:
3415 3138 424
AclI AA'CG_TT
Cuts at: 6817 6817
Size: 6977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 1176 3129 4802 5234 5587 6249 930
Size: 246 1953 1673 432 353 662 1658
Fragments arranged by size:
1953 1673 1658 662 432 353 246
AfeI AGC'GCT
Cuts at: 847 847
Size: 6977
AflIII A'CryG_T
Cuts at: 409 522 692 752 3005 4329 6129 6410 409
Size: 113 170 60 2253 1324 1800 281 976
Fragments arranged by size:
2253 1800 1324 976 281 170 113 60
AgeI A'CCGG_T
Cuts at: 2616 2616
Size: 6977
AhdI GACnn_n'nnGTC
Cuts at: 2865 2865
Size: 6977
AleI CACnn'nnGTG
Cuts at: 352 751 1069 2486 2617 352
Size: 399 318 1417 131 4712
Fragments arranged by size:
4712 1417 399 318 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 1613 2504 2608 3529 310
Size: 1303 891 104 921 3758
Fragments arranged by size:
3758 1303 921 891 104
AlwNI CAG_nnn'CTG
Cuts at: 312 1758 2526 4282 5720 312
Size: 1446 768 1756 1438 1569
Fragments arranged by size:
1756 1569 1446 1438 768
ApaI G_GGCC'C
Cuts at: 621 702 976 1220 1270 1433 1614 2180 2299
Size: 81 274 244 50 163 181 566 119
Cuts at: 2299 2441 3960 621
Size: 142 1519 3638
Fragments arranged by size:
3638 1519 566 274 244 181 163 142
119 81 50
ApaLI G'TGCA_C
Cuts at: 1517 1630 2479 3514 5815 1517
Size: 113 849 1035 2301 2679
Fragments arranged by size:
2679 2301 1035 849 113
ApoI r'AATT_y
Cuts at: 1090 3981 6330 6586 1090
Size: 2891 2349 256 1481
Fragments arranged by size:
2891 2349 1481 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6977
AvaI C'yCGr_G
Cuts at: 118 299 650 731 797 969 1219 1607 2044
Size: 181 351 81 66 172 250 388 437
Cuts at: 2044 2078 3038 3293 3319 3609 3823 3925 3997
Size: 34 960 255 26 290 214 102 72
Cuts at: 3997 118
Size: 3098
Fragments arranged by size:
3098 960 437 388 351 290 255 250
214 181 172 102 81 72 66 34
26
BamHI G'GATC_C
Cuts at: 4002 4002
Size: 6977
BanI G'GyrC_C
Cuts at: 154 346 439 550 930 2020 2488 2669 2740
Size: 192 93 111 380 1090 468 181 71
Cuts at: 2740 2814 3270 3569 3742 3993 4407 4655 4690
Size: 74 456 299 173 251 414 248 35
Cuts at: 4690 154
Size: 2441
Fragments arranged by size:
2441 1090 468 456 414 380 299 251
248 192 181 173 111 93 74 71
35
BanII G_rGCy'C
Cuts at: 621 702 976 1220 1236 1270 1433 1614 2180
Size: 81 274 244 16 34 163 181 566
Cuts at: 2180 2299 2441 2927 3610 3725 3960 3991 5021
Size: 119 142 486 683 115 235 31 1030
Cuts at: 5021 621
Size: 2577
Fragments arranged by size:
2577 1030 683 566 486 274 244 235
181 163 142 119 115 81 34 31
16
BbeI G_GCGC'C
Cuts at: 934 2818 3274 3746 4411 4659 934
Size: 1884 456 472 665 248 3252
Fragments arranged by size:
3252 1884 665 472 456 248
BbsI GAAGACnn'nnnn_
Cuts at: 1095 2029 2772 3074 1095
Size: 934 743 302 4998
Fragments arranged by size:
4998 934 743 302
BbvCI CC'TCA_GC
Cuts at: 1548 1548
Size: 6977
BciVI GTATCCnnnnn_n'
Cuts at: 4875 5931 6977 4875
Size: 1056 1046 4875
Fragments arranged by size:
4875 1056 1046
BclI T'GATC_A
Cuts at: 4497 4497
Size: 6977
BfrBI ATG'CAT
Cuts at: 1594 1594
Size: 6977
BglI GCCn_nnn'nGGC
Cuts at: 122 980 1390 1495 2812 2887 3391 3720 3964
Size: 858 410 105 1317 75 504 329 244
Cuts at: 3964 122
Size: 3135
Fragments arranged by size:
3135 1317 858 504 410 329 244 105
75
BglII A'GATC_T
Cuts at: 4492 6529 4492
Size: 2037 4940
BlpI GC'TnA_GC
Cuts at: 1450 1759 2354 2987 4089 1450
Size: 309 595 633 1102 4338
Fragments arranged by size:
4338 1102 633 595 309
Bme1580I G_kGCm'C
Cuts at: 184 194 349 479 553 621 702 806 976
Size: 10 155 130 74 68 81 104 170
Cuts at: 976 1220 1270 1433 1521 1614 1634 1861 2023
Size: 244 50 163 88 93 20 227 162
Cuts at: 2023 2165 2180 2299 2430 2441 2483 2672 2883
Size: 142 15 119 131 11 42 189 211
Cuts at: 2883 3518 3960 4602 4695 5819 184
Size: 635 442 642 93 1124 1342
Fragments arranged by size:
1342 1124 642 635 442 244 227 211
189 170 163 162 155 142 131 130
119 104 93 93 88 81 74 68
50 42 20 15 11 10
BmgBI CAC'GTC
Cuts at: 1061 1101 1686 2553 2949 3353 1061
Size: 40 585 867 396 404 4685
Fragments arranged by size:
4685 867 585 404 396 40
BmrI ACTGGGnnnn_n'
Cuts at: 171 2091 2567 3164 4189 4270 4605 6749 171
Size: 1920 476 597 1025 81 335 2144 399
Fragments arranged by size:
2144 1920 1025 597 476 399 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3078 5344 3078
Size: 2266 4711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 303 394 529 655 939 1117 1408 1477
Size: 11 91 135 126 284 178 291 69
Cuts at: 1477 1789 3073 3580 292
Size: 312 1284 507 3689
Fragments arranged by size:
3689 1284 507 312 291 284 178 135
126 91 69 11
Bpu10I CC'TnA_GC
Cuts at: 1548 1982 1548
Size: 434 6543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 4153 5032 5746 6044 871
Size: 3282 879 714 298 1804
Fragments arranged by size:
3282 1804 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 416 1726 2119 6681 416
Size: 1310 393 4562 712
Fragments arranged by size:
4562 1310 712 393
BsaAI yAC'GTr
Cuts at: 195 412 695 753 852 4330 195
Size: 217 283 58 99 3478 2842
Fragments arranged by size:
3478 2842 283 217 99 58
BsaBI GATnn'nnATC
Cuts at: 6 1289 4491 6528 6
Size: 1283 3202 2037 455
Fragments arranged by size:
3202 2037 1283 455
BsaHI Gr'CG_yC
Cuts at: 167 559 821 931 1124 2777 2815 3271 3644
Size: 392 262 110 193 1653 38 456 373
Cuts at: 3644 3743 3805 4408 4656 5358 6854 167
Size: 99 62 603 248 702 1496 290
Fragments arranged by size:
1653 1496 702 603 456 392 373 290
262 248 193 110 99 62 38
BsaWI w'CCGG_w
Cuts at: 2616 4166 4687 5365 5776 5923 6197 2616
Size: 1550 521 678 411 147 274 3396
Fragments arranged by size:
3396 1550 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1762 1792 2058 2088 3553 3583 1762
Size: 30 266 30 1465 30 5156
Fragments arranged by size:
5156 1465 266 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1474 1786 2803 3685 1474
Size: 312 1017 882 4766
Fragments arranged by size:
4766 1017 882 312
BseYI C'CCAG_C
Cuts at: 701 738 1084 1896 2151 2300 3490 4402 5160
Size: 37 346 812 255 149 1190 912 758
Cuts at: 5160 5825 6233 701
Size: 665 408 1445
Fragments arranged by size:
1445 1190 912 812 758 665 408 346
255 149 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 517 616 874 916 1762 1825 2527 2839
Size: 102 99 258 42 846 63 702 312
Cuts at: 2839 2974 3127 415
Size: 135 153 4265
Fragments arranged by size:
4265 846 702 312 258 153 135 102
99 63 42
BsiEI CG_ry'CG
Cuts at: 104 147 980 1990 2028 3780 4519 4565 5795
Size: 43 833 1010 38 1752 739 46 1230
Cuts at: 5795 104
Size: 1286
Fragments arranged by size:
1752 1286 1230 1010 833 739 46 43
38
BsiHKAI G_wGCw'C
Cuts at: 680 968 1100 1521 1634 1872 2483 2927 3350
Size: 288 132 421 113 238 611 444 423
Cuts at: 3350 3518 3932 3991 4769 4959 5819 680
Size: 168 414 59 778 190 860 1838
Fragments arranged by size:
1838 860 778 611 444 423 421 414
288 238 190 168 132 113 59
BsmBI CGTCTCn'nnnn_
Cuts at: 2494 3454 2494
Size: 960 6017
Bsp1286I G_dGCh'C
Cuts at: 184 194 349 479 553 621 680 702 806
Size: 10 155 130 74 68 59 22 104
Cuts at: 806 968 976 1100 1220 1236 1270 1433 1521
Size: 162 8 124 120 16 34 163 88
Cuts at: 1521 1614 1634 1861 1872 2023 2165 2180 2299
Size: 93 20 227 11 151 142 15 119
Cuts at: 2299 2430 2441 2483 2672 2883 2927 3350 3518
Size: 131 11 42 189 211 44 423 168
Cuts at: 3518 3610 3725 3932 3960 3991 4602 4695 4769
Size: 92 115 207 28 31 611 93 74
Cuts at: 4769 4959 5021 5819 184
Size: 190 62 798 1342
Fragments arranged by size:
1342 798 611 423 227 211 207 190
189 168 163 162 155 151 142 131
130 124 120 119 115 104 93 93
92 88 74 74 68 62 59 44
42 34 31 28 22 20 16 15
11 11 10 8
BspHI T'CATG_A
Cuts at: 2342 5409 2342
Size: 3067 3910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 593 705 888 1853 2147 2600 2861 3767
Size: 159 112 183 965 294 453 261 906
Cuts at: 3767 4027 4241 4543 4924 434
Size: 260 214 302 381 2487
Fragments arranged by size:
2487 965 906 453 381 302 294 261
260 214 183 159 112
BsrBI CCG'CTC
Cuts at: 50 378 390 1910 1950 1986 5269 5323 6807
Size: 328 12 1520 40 36 3283 54 1484
Cuts at: 6807 6972 50
Size: 165 55
Fragments arranged by size:
3283 1520 1484 328 165 55 54 40
36 12
BsrDI GCAATG_nn'
Cuts at: 2348 4889 2348
Size: 2541 4436
BsrFI r'CCGG_y
Cuts at: 283 979 1505 1989 2023 2616 3566 4975 5156
Size: 696 526 484 34 593 950 1409 181
Cuts at: 5156 283
Size: 2104
Fragments arranged by size:
2104 1409 950 696 593 526 484 181
34
BssSI C'ACGA_G
Cuts at: 5248 5956 5248
Size: 708 6269
BstAPI GCAn_nnn'nTGC
Cuts at: 4354 4354
Size: 6977
BstBI TT'CG_AA
Cuts at: 5338 5338
Size: 6977
BstEII G'GTnAC_C
Cuts at: 2238 3810 2238
Size: 1572 5405
BstXI CCAn_nnnn'nTGG
Cuts at: 451 780 2214 451
Size: 329 1434 5214
Fragments arranged by size:
5214 1434 329
BstYI r'GATC_y
Cuts at: 1 3554 4002 4469 4492 4827 5073 5379 5391
Size: 3553 448 467 23 335 246 306 12
Cuts at: 5391 5477 6529 1
Size: 86 1052 449
Fragments arranged by size:
3553 1052 467 449 448 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 485 1367 485
Size: 882 6095
BtgI C'CryG_G
Cuts at: 107 147 450 537 779 867 935 1064 1160
Size: 40 303 87 242 88 68 129 96
Cuts at: 1160 1469 2172 2267 3187 3726 3747 3968 107
Size: 309 703 95 920 539 21 221 3116
Fragments arranged by size:
3116 920 703 539 309 303 242 221
129 96 95 88 87 68 40 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1587 4927 5108 5271 1587
Size: 3340 181 163 3293
Fragments arranged by size:
3340 3293 181 163
BtsI GCAGTG_nn'
Cuts at: 367 1783 2037 4220 367
Size: 1416 254 2183 3124
Fragments arranged by size:
3124 2183 1416 254
DraI TTT'AAA
Cuts at: 3976 3976
Size: 6977
DrdI GACnn_nn'nnGTC
Cuts at: 4683 6027 4683
Size: 1344 5633
EaeI y'GGCC_r
Cuts at: 356 470 977 1026 1397 1466 1487 1987 2025
Size: 114 507 49 371 69 21 500 38
Cuts at: 2025 2626 2657 2993 3712 3777 4562 4736 5127
Size: 601 31 336 719 65 785 174 391
Cuts at: 5127 5154 6499 356
Size: 27 1345 834
Fragments arranged by size:
1345 834 785 719 601 507 500 391
371 336 174 114 69 65 49 38
31 27 21
EagI C'GGCC_G
Cuts at: 977 1987 2025 3777 4562 977
Size: 1010 38 1752 785 3392
Fragments arranged by size:
3392 1752 1010 785 38
EarI CTCTTCn'nnn_
Cuts at: 494 1192 5000 5210 494
Size: 698 3808 210 2261
Fragments arranged by size:
3808 2261 698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 250 3085 3195 5185 5927 6073 6787 250
Size: 2835 110 1990 742 146 714 440
Fragments arranged by size:
2835 1990 742 714 440 146 110
EcoICRI GAG'CTC
Cuts at: 2925 3989 2925
Size: 1064 5913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 303 394 529 655 930 939 1117 1176
Size: 11 91 135 126 275 9 178 59
Cuts at: 1176 1408 1477 1789 3073 3129 3580 4802 5234
Size: 232 69 312 1284 56 451 1222 432
Cuts at: 5234 5587 6249 292
Size: 353 662 1020
Fragments arranged by size:
1284 1222 1020 662 451 432 353 312
275 232 178 135 126 91 69 59
56 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 274 566 1459 3121 274
Size: 292 893 1662 4130
Fragments arranged by size:
4130 1662 893 292
EcoO109I rG'GnC_Cy
Cuts at: 222 456 582 617 618 1137 1243 1266 1267
Size: 234 126 35 1 519 106 23 1
Cuts at: 1267 1430 1611 1814 2177 2296 2805 2826 3042
Size: 163 181 203 363 119 509 21 216
Cuts at: 3042 3383 3392 3487 3870 3879 4116 222
Size: 341 9 95 383 9 237 3083
Fragments arranged by size:
3083 519 509 383 363 341 237 234
216 203 181 163 126 119 106 95
35 23 21 9 9 1 1
EcoRI G'AATT_C
Cuts at: 1090 3981 1090
Size: 2891 4086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4996 4996
Size: 6977
FspI TGC'GCA
Cuts at: 787 4758 787
Size: 3971 3006
FspAI rTGC'GCAy
Cuts at: 787 787
Size: 6977
HaeII r_GCGC'y
Cuts at: 400 849 934 2818 3134 3274 3679 3702 3746
Size: 449 85 1884 316 140 405 23 44
Cuts at: 3746 4411 4659 5889 6660 400
Size: 665 248 1230 771 717
Fragments arranged by size:
1884 1230 771 717 665 449 405 316
248 140 85 44 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2058 2090 2553 2585 2851 2883 3287 3319 3774
Size: 32 463 32 266 32 404 32 455
Cuts at: 3774 3806 4819 4849 4851 4881 2058
Size: 32 1013 30 2 30 4154
Fragments arranged by size:
4154 1013 463 455 404 266 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1410 4016 1410
Size: 2606 4371
KasI G'GCGC_C
Cuts at: 930 2814 3270 3742 4407 4655 930
Size: 1884 456 472 665 248 3252
Fragments arranged by size:
3252 1884 665 472 456 248
KpnI G_GTAC'C
Cuts at: 158 3573 3997 158
Size: 3415 424 3138
Fragments arranged by size:
3415 3138 424
MluI A'CGCG_T
Cuts at: 522 6410 522
Size: 5888 1089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 5736 5920 2849
Size: 2887 184 3906
Fragments arranged by size:
3906 2887 184
MscI TGG'CCA
Cuts at: 358 1028 1399 1468 1489 2659 4738 358
Size: 670 371 69 21 1170 2079 2597
Fragments arranged by size:
2597 2079 1170 670 371 69 21
MslI CAynn'nnrTG
Cuts at: 340 352 751 778 1069 1595 2212 2266 2476
Size: 12 399 27 291 526 617 54 210
Cuts at: 2476 2486 2573 2617 4966 5093 340
Size: 10 87 44 2349 127 2224
Fragments arranged by size:
2349 2224 617 526 399 291 210 127
87 54 44 27 12 10
MspA1I CmG'CkG
Cuts at: 312 950 1120 1146 1168 1449 1746 2304 2319
Size: 638 170 26 22 281 297 558 15
Cuts at: 2319 2840 2913 3149 3189 3494 3509 3749 4088
Size: 521 73 236 40 305 15 240 339
Cuts at: 4088 4402 4762 5546 5791 312
Size: 314 360 784 245 1498
Fragments arranged by size:
1498 784 638 558 521 360 339 314
305 297 281 245 240 236 170 73
40 26 22 15 15
NaeI GCC'GGC
Cuts at: 285 981 1507 5158 285
Size: 696 526 3651 2104
Fragments arranged by size:
3651 2104 696 526
NarI GG'CG_CC
Cuts at: 931 2815 3271 3743 4408 4656 931
Size: 1884 456 472 665 248 3252
Fragments arranged by size:
3252 1884 665 472 456 248
NcoI C'CATG_G
Cuts at: 107 779 935 1469 2172 107
Size: 672 156 534 703 4912
Fragments arranged by size:
4912 703 672 534 156
NgoMIV G'CCGG_C
Cuts at: 283 979 1505 5156 283
Size: 696 526 3651 2104
Fragments arranged by size:
3651 2104 696 526
NsiI A_TGCA'T
Cuts at: 1596 1596
Size: 6977
NspI r_CATG'y
Cuts at: 291 1594 1598 3009 4030 4530 6133 291
Size: 1303 4 1411 1021 500 1603 1135
Fragments arranged by size:
1603 1411 1303 1135 1021 500 4
PasI CC'CwG_GG
Cuts at: 184 1614 1665 1800 3408 3432 184
Size: 1430 51 135 1608 24 3729
Fragments arranged by size:
3729 1608 1430 135 51 24
PciI A'CATG_T
Cuts at: 3005 6129 3005
Size: 3124 3853
PflMI CCAn_nnn'nTGG
Cuts at: 312 1560 2916 312
Size: 1248 1356 4373
Fragments arranged by size:
4373 1356 1248
PfoI T'CCnGG_A
Cuts at: 269 2783 3305 3557 269
Size: 2514 522 252 3689
Fragments arranged by size:
3689 2514 522 252
PmeI GTTT'AAAC
Cuts at: 3976 3976
Size: 6977
PmlI CAC'GTG
Cuts at: 195 412 695 753 195
Size: 217 283 58 6419
Fragments arranged by size:
6419 283 217 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5423 5455 5423
Size: 32 6945
PpuMI rG'GwC_Cy
Cuts at: 222 456 582 1243 1814 2826 3487 3870 3879
Size: 234 126 661 571 1012 661 383 9
Cuts at: 3879 222
Size: 3320
Fragments arranged by size:
3320 1012 661 661 571 383 234 126
9
PshAI GACnn'nnGTC
Cuts at: 320 320
Size: 6977
PspOMI G'GGCC_C
Cuts at: 617 698 972 1216 1266 1429 1610 2176 2295
Size: 81 274 244 50 163 181 566 119
Cuts at: 2295 2437 3956 617
Size: 142 1519 3638
Fragments arranged by size:
3638 1519 566 274 244 181 163 142
119 81 50
PspXI vC'TCGA_Gb
Cuts at: 2044 2044
Size: 6977
PstI C_TGCA'G
Cuts at: 1796 2378 2839 4024 4709 1796
Size: 582 461 1185 685 4064
Fragments arranged by size:
4064 1185 685 582 461
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6977
PvuII CAG'CTG
Cuts at: 1120 1146 1746 2304 2319 2913 3494 4402 4762
Size: 26 600 558 15 594 581 908 360
Cuts at: 4762 1120
Size: 3335
Fragments arranged by size:
3335 908 600 594 581 558 360 26
15
RsrII CG'GwC_CG
Cuts at: 5172 5172
Size: 6977
SacI G_AGCT'C
Cuts at: 2927 3991 2927
Size: 1064 5913
SacII CC_GC'GG
Cuts at: 3190 3750 3190
Size: 560 6417
SalI G'TCGA_C
Cuts at: 4014 4014
Size: 6977
SanDI GG'GwC_CC
Cuts at: 3487 3870 3487
Size: 383 6594
SapI GCTCTTCn'nnn_
Cuts at: 5000 5210 5000
Size: 210 6767
SbfI CC_TGCA'GG
Cuts at: 4024 4024
Size: 6977
ScaI AGT'ACT
Cuts at: 6494 6494
Size: 6977
SfcI C'TryA_G
Cuts at: 33 1792 2374 2835 4020 4705 5673 5864 33
Size: 1759 582 461 1185 685 968 191 1146
Fragments arranged by size:
1759 1185 1146 968 685 582 461 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1495 3391 3964 1495
Size: 1896 573 4508
Fragments arranged by size:
4508 1896 573
SfoI GGC'GCC
Cuts at: 932 2816 3272 3744 4409 4657 932
Size: 1884 456 472 665 248 3252
Fragments arranged by size:
3252 1884 665 472 456 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6977
SgrAI Cr'CCGG_yG
Cuts at: 2616 2616
Size: 6977
SmaI CCC'GGG
Cuts at: 301 733 1609 2080 3040 3825 3999 301
Size: 432 876 471 960 785 174 3279
Fragments arranged by size:
3279 960 876 785 471 432 174
SmlI C'TyrA_G
Cuts at: 650 886 2044 4132 5047 5761 6023 650
Size: 236 1158 2088 915 714 262 1604
Fragments arranged by size:
2088 1604 1158 915 714 262 236
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6977
SphI G_CATG'C
Cuts at: 291 1594 4030 4530 291
Size: 1303 2436 500 2738
Fragments arranged by size:
2738 2436 1303 500
SrfI GCCC'GGGC
Cuts at: 1609 1609
Size: 6977
StuI AGG'CCT
Cuts at: 1372 2959 1372
Size: 1587 5390
StyI C'CwwG_G
Cuts at: 107 779 935 1469 2172 4111 4465 107
Size: 672 156 534 703 1939 354 2619
Fragments arranged by size:
2619 1939 703 672 534 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 457 709 1253 1910 2476 3709 4560 4693 5152
Size: 252 544 657 566 1233 851 133 459
Cuts at: 5152 5361 457
Size: 209 2073
Fragments arranged by size:
2073 1233 851 657 566 544 459 252
209 133
TatI w'GTAC_w
Cuts at: 6492 6492
Size: 6977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 401 433 1762 1794 6494 6526 401
Size: 32 1329 32 4700 32 852
Fragments arranged by size:
4700 1329 852 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3643 4774 3643
Size: 1131 5846
XbaI T'CTAG_A
Cuts at: 4008 4008
Size: 6977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 369 1021 1645 4472 339
Size: 30 652 624 2827 2844
Fragments arranged by size:
2844 2827 652 624 30
XhoI C'TCGA_G
Cuts at: 650 2044 650
Size: 1394 5583
XmaI C'CCGG_G
Cuts at: 299 731 1607 2078 3038 3823 3997 299
Size: 432 876 471 960 785 174 3279
Fragments arranged by size:
3279 960 876 785 471 432 174
ZraI GAC'GTC
Cuts at: 168 3806 168
Size: 3638 3339
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AflII AloI AscI AvrII BaeI BcgI BmtI BsiWI
BsmI BspEI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PsiI PsrI SexAI SnaBI SspI SwaI
XmnI