Restriction map for
pF1KB7302
(Circular) (Six-base) MAPSORT of: pF1KB7302.seq Check: 4942 from: 1 to: 6866
pF1KB7302 6866 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1248 1500 1248
Size: 252 6614
AatII G_ACGT'C
Cuts at: 1880 3590 1880
Size: 1710 5156
AccI GT'mk_AC
Cuts at: 3053 3904 3053
Size: 851 6015
Acc65I G'GTAC_C
Cuts at: 3882 3882
Size: 6866
AclI AA'CG_TT
Cuts at: 6706 6706
Size: 6866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1292 1379 1415 1694 1976 2326 3052 4691 5123
Size: 87 36 279 282 350 726 1639 432
Cuts at: 5123 5870 6138 1292
Size: 747 268 2020
Fragments arranged by size:
2020 1639 747 726 432 350 282 279
268 87 36
AflIII A'CryG_T
Cuts at: 2468 4218 5322 6299 2468
Size: 1750 1104 977 3035
Fragments arranged by size:
3035 1750 1104 977
AgeI A'CCGG_T
Cuts at: 5254 5254
Size: 6866
AlwNI CAG_nnn'CTG
Cuts at: 492 603 676 1320 1818 2694 3819 4171 5738
Size: 111 73 644 498 876 1125 352 1567
Cuts at: 5738 492
Size: 1620
Fragments arranged by size:
1620 1567 1125 876 644 498 352 111
73
ApaI G_GGCC'C
Cuts at: 369 722 3510 3799 369
Size: 353 2788 289 3436
Fragments arranged by size:
3436 2788 353 289
ApaLI G'TGCA_C
Cuts at: 522 5636 522
Size: 5114 1752
ApoI r'AATT_y
Cuts at: 2520 2820 3870 6219 6475 2520
Size: 300 1050 2349 256 2911
Fragments arranged by size:
2911 2349 1050 300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
AvaI C'yCGr_G
Cuts at: 734 1629 3410 3676 3684 3792 3886 734
Size: 895 1781 266 8 108 94 3714
Fragments arranged by size:
3714 1781 895 266 108 94 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1076 1109 1076
Size: 33 6833
BamHI G'GATC_C
Cuts at: 3891 3891
Size: 6866
BanI G'GyrC_C
Cuts at: 795 3418 3882 4296 4544 4579 795
Size: 2623 464 414 248 35 3082
Fragments arranged by size:
3082 2623 464 414 248 35
BanII G_rGCy'C
Cuts at: 369 722 812 1418 1682 1900 3510 3580 3683
Size: 353 90 606 264 218 1610 70 103
Cuts at: 3683 3703 3761 3793 3799 3880 4910 369
Size: 20 58 32 6 81 1030 2325
Fragments arranged by size:
2325 1610 1030 606 353 264 218 103
90 81 70 58 32 20 6
BbeI G_GCGC'C
Cuts at: 4300 4548 4300
Size: 248 6618
BbsI GAAGACnn'nnnn_
Cuts at: 166 1289 2635 2676 166
Size: 1123 1346 41 4356
Fragments arranged by size:
4356 1346 1123 41
BbvCI CC'TCA_GC
Cuts at: 137 620 1454 2455 137
Size: 483 834 1001 4548
Fragments arranged by size:
4548 1001 834 483
BciVI GTATCCnnnnn_n'
Cuts at: 4764 5525 6866 4764
Size: 761 1341 4764
Fragments arranged by size:
4764 1341 761
BclI T'GATC_A
Cuts at: 4386 4386
Size: 6866
BglI GCCn_nnn'nGGC
Cuts at: 550 1774 550
Size: 1224 5642
BglII A'GATC_T
Cuts at: 4381 6418 4381
Size: 2037 4829
BlpI GC'TnA_GC
Cuts at: 1623 2335 3978 1623
Size: 712 1643 4511
Fragments arranged by size:
4511 1643 712
Bme1580I G_kGCm'C
Cuts at: 369 526 722 3421 3510 3799 4491 4584 5640
Size: 157 196 2699 89 289 692 93 1056
Cuts at: 5640 369
Size: 1595
Fragments arranged by size:
2699 1595 1056 692 289 196 157 93
89
BmgBI CAC'GTC
Cuts at: 2961 2961
Size: 6866
BmrI ACTGGGnnnn_n'
Cuts at: 518 1434 1702 2547 2583 4078 4159 4494 6638
Size: 916 268 845 36 1495 81 335 2144
Cuts at: 6638 518
Size: 746
Fragments arranged by size:
2144 1495 916 845 746 335 268 81
36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 762 969 3621 5233 762
Size: 207 2652 1612 2395
Fragments arranged by size:
2652 2395 1612 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 666 680 788 916 1016 1795 1922 3553
Size: 53 14 108 128 100 779 127 1631
Cuts at: 3553 613
Size: 3926
Fragments arranged by size:
3926 1631 779 128 127 108 100 53
14
Bpu10I CC'TnA_GC
Cuts at: 137 620 1454 2455 137
Size: 483 834 1001 4548
Fragments arranged by size:
4548 1001 834 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1666 4042 4921 5413 5711 568
Size: 1098 2376 879 492 298 1723
Fragments arranged by size:
2376 1723 1098 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 733 1426 3695 6570 319
Size: 414 693 2269 2875 615
Fragments arranged by size:
2875 2269 693 615 414
BsaAI yAC'GTr
Cuts at: 2469 4219 2469
Size: 1750 5116
BsaBI GATnn'nnATC
Cuts at: 6 3106 4380 6417 6
Size: 3100 1274 2037 455
Fragments arranged by size:
3100 2037 1274 455
BsaHI Gr'CG_yC
Cuts at: 702 1877 2711 3587 3670 4297 4545 5247 6743
Size: 1175 834 876 83 627 248 702 1496
Cuts at: 6743 702
Size: 825
Fragments arranged by size:
1496 1175 876 834 825 702 627 248
83
BsaWI w'CCGG_w
Cuts at: 3335 4055 4576 5254 5528 5675 6086 3335
Size: 720 521 678 274 147 411 4115
Fragments arranged by size:
4115 720 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 989 1019 989
Size: 30 6836
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 304 828 1042 1177 1192 1195 1210 1213
Size: 51 524 214 135 15 3 15 3
Cuts at: 1213 1216 1219 1228 1231 1354 1390 1399 1414
Size: 3 3 9 3 123 36 9 15
Cuts at: 1414 1456 1906 1990 2377 3397 3754 3757 3862
Size: 42 450 84 387 1020 357 3 105
Cuts at: 3862 253
Size: 3257
Fragments arranged by size:
3257 1020 524 450 387 357 214 135
123 105 84 51 42 36 15 15
15 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 116 400 687 812 1911 2138 4291 5049 5626
Size: 284 287 125 1099 227 2153 758 577
Cuts at: 5626 6122 116
Size: 496 860
Fragments arranged by size:
2153 1099 860 758 577 496 287 284
227 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 994 1528 2866 2941 3148 871
Size: 123 534 1338 75 207 4589
Fragments arranged by size:
4589 1338 534 207 123 75
BsiEI CG_ry'CG
Cuts at: 104 1806 3096 3755 4408 4454 5662 104
Size: 1702 1290 659 653 46 1208 1308
Fragments arranged by size:
1702 1308 1290 1208 659 653 46
BsiHKAI G_wGCw'C
Cuts at: 526 1306 1682 1982 3580 3703 3770 3880 4658
Size: 780 376 300 1598 123 67 110 778
Cuts at: 4658 4848 5640 526
Size: 190 792 1752
Fragments arranged by size:
1752 1598 792 780 778 376 300 190
123 110 67
BsmBI CGTCTCn'nnnn_
Cuts at: 1592 1592
Size: 6866
Bsp1286I G_dGCh'C
Cuts at: 369 526 722 812 1306 1418 1682 1900 1982
Size: 157 196 90 494 112 264 218 82
Cuts at: 1982 3421 3510 3580 3683 3703 3761 3770 3793
Size: 1439 89 70 103 20 58 9 23
Cuts at: 3793 3799 3880 4491 4584 4658 4848 4910 5640
Size: 6 81 611 93 74 190 62 730
Cuts at: 5640 369
Size: 1595
Fragments arranged by size:
1595 1439 730 611 494 264 218 196
190 157 112 103 93 90 89 82
81 74 70 62 58 23 20 9
6
BspEI T'CCGG_A
Cuts at: 3335 6086 3335
Size: 2751 4115
BspHI T'CATG_A
Cuts at: 425 6042 425
Size: 5617 1249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1248 1500 2339 3065 3120 3916 4130 4432 4813
Size: 252 839 726 55 796 214 302 381
Cuts at: 4813 1248
Size: 3301
Fragments arranged by size:
3301 839 796 726 381 302 252 214
55
BsrBI CCG'CTC
Cuts at: 50 1839 5158 5212 6696 6861 50
Size: 1789 3319 54 1484 165 55
Fragments arranged by size:
3319 1789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4778 4778
Size: 6866
BsrFI r'CCGG_y
Cuts at: 1805 3008 3227 3750 4864 5045 5254 1805
Size: 1203 219 523 1114 181 209 3417
Fragments arranged by size:
3417 1203 1114 523 219 209 181
BsrGI T'GTAC_A
Cuts at: 2471 3104 2471
Size: 633 6233
BssSI C'ACGA_G
Cuts at: 5137 5495 5137
Size: 358 6508
BstAPI GCAn_nnn'nTGC
Cuts at: 918 2895 4243 918
Size: 1977 1348 3541
Fragments arranged by size:
3541 1977 1348
BstBI TT'CG_AA
Cuts at: 5227 5227
Size: 6866
BstXI CCAn_nnnn'nTGG
Cuts at: 873 3246 873
Size: 2373 4493
BstYI r'GATC_y
Cuts at: 1 488 2831 3181 3779 3815 3891 4358 4381
Size: 487 2343 350 598 36 76 467 23
Cuts at: 4381 4716 4962 5974 6060 6072 6418 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2343 1012 598 487 467 449 350 346
335 246 86 76 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 236 320 1448 236
Size: 84 1128 5654
Fragments arranged by size:
5654 1128 84
BtgI C'CryG_G
Cuts at: 1772 1772
Size: 6866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4816 4997 5160 4816
Size: 181 163 6522
Fragments arranged by size:
6522 181 163
BtsI GCAGTG_nn'
Cuts at: 4109 4109
Size: 6866
DraI TTT'AAA
Cuts at: 2408 3865 2408
Size: 1457 5409
DraIII CAC_nnn'GTG
Cuts at: 521 3774 521
Size: 3253 3613
DrdI GACnn_nn'nnGTC
Cuts at: 1876 4572 5430 1876
Size: 2696 858 3312
Fragments arranged by size:
3312 2696 858
EaeI y'GGCC_r
Cuts at: 1752 1775 3093 3236 3524 3752 4451 4625 5016
Size: 23 1318 143 288 228 699 174 391
Cuts at: 5016 5043 6388 1752
Size: 27 1345 2230
Fragments arranged by size:
2230 1345 1318 699 391 288 228 174
143 27 23
EagI C'GGCC_G
Cuts at: 3093 3752 4451 3093
Size: 659 699 5508
Fragments arranged by size:
5508 699 659
EarI CTCTTCn'nnn_
Cuts at: 173 269 1106 1154 1181 1187 1205 1355 1391
Size: 96 837 48 27 6 18 150 36
Cuts at: 1391 1547 1670 1834 1967 3028 3274 3382 3436
Size: 156 123 164 133 1061 246 108 54
Cuts at: 3436 3758 4889 5099 173
Size: 322 1131 210 1940
Fragments arranged by size:
1940 1131 1061 837 322 246 210 164
156 150 133 123 108 96 54 48
36 27 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 226 1444 2380 3597 3871 5074 5384 5530 6676
Size: 1218 936 1217 274 1203 310 146 1146
Cuts at: 6676 226
Size: 416
Fragments arranged by size:
1218 1217 1203 1146 936 416 310 274
146
EcoICRI GAG'CTC
Cuts at: 1680 3578 3701 3878 1680
Size: 1898 123 177 4668
Fragments arranged by size:
4668 1898 177 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 666 680 788 916 1016 1292 1379 1415
Size: 53 14 108 128 100 276 87 36
Cuts at: 1415 1694 1795 1922 1976 2326 3052 3553 4691
Size: 279 101 127 54 350 726 501 1138
Cuts at: 4691 5123 5870 6138 613
Size: 432 747 268 1341
Fragments arranged by size:
1341 1138 747 726 501 432 350 279
276 268 128 127 108 101 100 87
54 53 36 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1027 1027
Size: 6866
EcoO109I rG'GnC_Cy
Cuts at: 149 477 543 718 858 882 1289 2556 3288
Size: 328 66 175 140 24 407 1267 732
Cuts at: 3288 4005 149
Size: 717 3010
Fragments arranged by size:
3010 1267 732 717 407 328 175 140
66 24
EcoRI G'AATT_C
Cuts at: 2520 3870 2520
Size: 1350 5516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2688 4885 2688
Size: 2197 4669
FseI GG_CCGG'CC
Cuts at: 3754 3754
Size: 6866
FspI TGC'GCA
Cuts at: 4647 4647
Size: 6866
HaeII r_GCGC'y
Cuts at: 377 694 1793 4300 4548 5570 6549 377
Size: 317 1099 2507 248 1022 979 694
Fragments arranged by size:
2507 1099 1022 979 694 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 244 270 276 1867 1899 3139 3171 3800
Size: 6 26 6 1591 32 1240 32 629
Cuts at: 3800 3832 4708 4738 4740 4770 238
Size: 32 876 30 2 30 2334
Fragments arranged by size:
2334 1591 1240 876 629 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 3905 3905
Size: 6866
HindIII A'AGCT_T
Cuts at: 3201 3201
Size: 6866
KasI G'GCGC_C
Cuts at: 4296 4544 4296
Size: 248 6618
KpnI G_GTAC'C
Cuts at: 3886 3886
Size: 6866
MluI A'CGCG_T
Cuts at: 6299 6299
Size: 6866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5537 5721 5537
Size: 184 6682
MscI TGG'CCA
Cuts at: 1754 4627 1754
Size: 2873 3993
MslI CAynn'nnrTG
Cuts at: 1061 2227 2959 4855 4982 1061
Size: 1166 732 1896 127 2945
Fragments arranged by size:
2945 1896 1166 732 127
MspA1I CmG'CkG
Cuts at: 1954 2058 2402 2691 2792 3977 4291 4651 5664
Size: 104 344 289 101 1185 314 360 1013
Cuts at: 5664 5909 1954
Size: 245 2911
Fragments arranged by size:
2911 1185 1013 360 344 314 289 245
104 101
NaeI GCC'GGC
Cuts at: 3752 5047 3752
Size: 1295 5571
NarI GG'CG_CC
Cuts at: 4297 4545 4297
Size: 248 6618
NgoMIV G'CCGG_C
Cuts at: 3750 5045 3750
Size: 1295 5571
NspI r_CATG'y
Cuts at: 1995 2292 2898 3030 3357 3919 4419 5326 1995
Size: 297 606 132 327 562 500 907 3535
Fragments arranged by size:
3535 907 606 562 500 327 297 132
PasI CC'CwG_GG
Cuts at: 145 369 958 1043 145
Size: 224 589 85 5968
Fragments arranged by size:
5968 589 224 85
PciI A'CATG_T
Cuts at: 5322 5322
Size: 6866
PflMI CCAn_nnn'nTGG
Cuts at: 714 1818 2573 714
Size: 1104 755 5007
Fragments arranged by size:
5007 1104 755
PfoI T'CCnGG_A
Cuts at: 696 993 1150 1811 2216 3275 696
Size: 297 157 661 405 1059 4287
Fragments arranged by size:
4287 1059 661 405 297 157
PmeI GTTT'AAAC
Cuts at: 3865 3865
Size: 6866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6005 6037 6005
Size: 32 6834
PpuMI rG'GwC_Cy
Cuts at: 149 477 858 882 1289 3288 149
Size: 328 381 24 407 1999 3727
Fragments arranged by size:
3727 1999 407 381 328 24
PspOMI G'GGCC_C
Cuts at: 365 718 3506 3795 365
Size: 353 2788 289 3436
Fragments arranged by size:
3436 2788 353 289
PstI C_TGCA'G
Cuts at: 663 731 773 2249 3913 4598 663
Size: 68 42 1476 1664 685 2931
Fragments arranged by size:
2931 1664 1476 685 68 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6866
PvuII CAG'CTG
Cuts at: 2691 2792 4291 4651 2691
Size: 101 1499 360 4906
Fragments arranged by size:
4906 1499 360 101
RsrII CG'GwC_CG
Cuts at: 5061 5061
Size: 6866
SacI G_AGCT'C
Cuts at: 1682 3580 3703 3880 1682
Size: 1898 123 177 4668
Fragments arranged by size:
4668 1898 177 123
SalI G'TCGA_C
Cuts at: 3903 3903
Size: 6866
SanDI GG'GwC_CC
Cuts at: 149 858 1289 149
Size: 709 431 5726
Fragments arranged by size:
5726 709 431
SapI GCTCTTCn'nnn_
Cuts at: 1670 1834 3382 3436 3758 4889 5099 1670
Size: 164 1548 54 322 1131 210 3437
Fragments arranged by size:
3437 1548 1131 322 210 164 54
SbfI CC_TGCA'GG
Cuts at: 3913 3913
Size: 6866
ScaI AGT'ACT
Cuts at: 6383 6383
Size: 6866
SexAI A'CCwGG_T
Cuts at: 2863 2863
Size: 6866
SfcI C'TryA_G
Cuts at: 33 659 668 727 769 2245 2505 3909 4594
Size: 626 9 59 42 1476 260 1404 685
Cuts at: 4594 5587 5778 33
Size: 993 191 1121
Fragments arranged by size:
1476 1404 1121 993 685 626 260 191
59 42 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 550 1774 550
Size: 1224 5642
SfoI GGC'GCC
Cuts at: 4298 4546 4298
Size: 248 6618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6866
SmaI CCC'GGG
Cuts at: 736 3412 3686 3794 3888 736
Size: 2676 274 108 94 3714
Fragments arranged by size:
3714 2676 274 108 94
SmlI C'TyrA_G
Cuts at: 547 1681 4021 4936 5428 5690 547
Size: 1134 2340 915 492 262 1723
Fragments arranged by size:
2340 1723 1134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6866
SphI G_CATG'C
Cuts at: 2898 3357 3919 4419 2898
Size: 459 562 500 5345
Fragments arranged by size:
5345 562 500 459
SrfI GCCC'GGGC
Cuts at: 3794 3794
Size: 6866
StuI AGG'CCT
Cuts at: 567 567
Size: 6866
StyI C'CwwG_G
Cuts at: 329 776 788 1370 1436 2348 3377 3720 4000
Size: 447 12 582 66 912 1029 343 280
Cuts at: 4000 4354 329
Size: 354 2841
Fragments arranged by size:
2841 1029 912 582 447 354 343 280
66 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 774 1514 3454 3647 4449 4582 5041 5250 774
Size: 740 1940 193 802 133 459 209 2390
Fragments arranged by size:
2390 1940 802 740 459 209 193 133
TatI w'GTAC_w
Cuts at: 2411 2471 2813 2873 3104 6381 2411
Size: 60 342 60 231 3277 2896
Fragments arranged by size:
3277 2896 342 231 60 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 505 537 989 1021 6383 6415 505
Size: 32 452 32 5362 32 956
Fragments arranged by size:
5362 956 452 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 252 1018 4663 252
Size: 766 3645 2455
Fragments arranged by size:
3645 2455 766
XbaI T'CTAG_A
Cuts at: 3897 3897
Size: 6866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1715 4361 1715
Size: 2646 4220
XmaI C'CCGG_G
Cuts at: 734 3410 3684 3792 3886 734
Size: 2676 274 108 94 3714
Fragments arranged by size:
3714 2676 274 108 94
XmnI GAAnn'nnTTC
Cuts at: 1828 2701 1828
Size: 873 5993
ZraI GAC'GTC
Cuts at: 1878 3588 1878
Size: 1710 5156
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BcgI BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
ClaI CspCI EcoRV FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SgrAI SnaBI SspI SwaI XhoI