Restriction map for
pF1KB7300
(Circular) (Six-base) MAPSORT of: pF1KB7300.seq Check: 6092 from: 1 to: 5981
pF1KB7300 5981 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2529 2861 2529
Size: 332 5649
AccI GT'mk_AC
Cuts at: 1940 3019 1940
Size: 1079 4902
Acc65I G'GTAC_C
Cuts at: 1618 2848 2997 1618
Size: 1230 149 4602
Fragments arranged by size:
4602 1230 149
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 658 2084 2343 3806 4238 4591 5253 251
Size: 407 1426 259 1463 432 353 662 979
Fragments arranged by size:
1463 1426 979 662 432 407 353 259
AflIII A'CryG_T
Cuts at: 911 3333 5133 5414 911
Size: 2422 1800 281 1478
Fragments arranged by size:
2422 1800 1478 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 386 1206 2661 386
Size: 820 1455 3706
Fragments arranged by size:
3706 1455 820
AlwNI CAG_nnn'CTG
Cuts at: 665 1692 3286 4724 665
Size: 1027 1594 1438 1922
Fragments arranged by size:
1922 1594 1438 1027
ApaI G_GGCC'C
Cuts at: 2142 2573 2142
Size: 431 5550
ApaLI G'TGCA_C
Cuts at: 4819 4819
Size: 5981
ApoI r'AATT_y
Cuts at: 2985 5334 5590 2985
Size: 2349 256 3376
Fragments arranged by size:
3376 2349 256
AseI AT'TA_AT
Cuts at: 20 1009 20
Size: 989 4992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 621 2572 3001 621
Size: 1951 429 3601
Fragments arranged by size:
3601 1951 429
BamHI G'GATC_C
Cuts at: 3006 3006
Size: 5981
BanI G'GyrC_C
Cuts at: 124 1033 1241 1618 1878 1930 2848 2967 2997
Size: 909 208 377 260 52 918 119 30
Cuts at: 2997 3411 3659 3694 124
Size: 414 248 35 2411
Fragments arranged by size:
2411 918 909 414 377 260 248 208
119 52 35 30
BanII G_rGCy'C
Cuts at: 311 563 672 1710 1849 2142 2573 2861 2995
Size: 252 109 1038 139 293 431 288 134
Cuts at: 2995 4025 311
Size: 1030 2267
Fragments arranged by size:
2267 1038 1030 431 293 288 252 139
134 109
BbeI G_GCGC'C
Cuts at: 128 3415 3663 128
Size: 3287 248 2446
Fragments arranged by size:
3287 2446 248
BbsI GAAGACnn'nnnn_
Cuts at: 268 268
Size: 5981
BbvCI CC'TCA_GC
Cuts at: 323 544 2316 2335 2777 323
Size: 221 1772 19 442 3527
Fragments arranged by size:
3527 1772 442 221 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1023 1057 90
Size: 34 899 34 5014
Fragments arranged by size:
5014 899 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3879 4935 5981 3879
Size: 1056 1046 3879
Fragments arranged by size:
3879 1056 1046
BclI T'GATC_A
Cuts at: 2438 3501 2438
Size: 1063 4918
BglI GCCn_nnn'nGGC
Cuts at: 168 1138 1204 2132 2171 168
Size: 970 66 928 39 3978
Fragments arranged by size:
3978 970 928 66 39
BglII A'GATC_T
Cuts at: 598 3496 5533 598
Size: 2898 2037 1046
Fragments arranged by size:
2898 2037 1046
BlpI GC'TnA_GC
Cuts at: 305 496 3093 305
Size: 191 2597 3193
Fragments arranged by size:
3193 2597 191
Bme1580I G_kGCm'C
Cuts at: 1244 1883 1933 2142 2573 2972 3606 3699 4823
Size: 639 50 209 431 399 634 93 1124
Cuts at: 4823 1244
Size: 2402
Fragments arranged by size:
2402 1124 639 634 431 399 209 93
50
BmgBI CAC'GTC
Cuts at: 1938 1938
Size: 5981
BmrI ACTGGGnnnn_n'
Cuts at: 675 1247 2701 3193 3274 3609 5753 675
Size: 572 1454 492 81 335 2144 903
Fragments arranged by size:
2144 1454 903 572 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 4348 2630
Size: 1718 4263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1106 1885 2246 2477 2652 2845 370
Size: 736 779 361 231 175 193 3506
Fragments arranged by size:
3506 779 736 361 231 193 175
Bpu10I CC'TnA_GC
Cuts at: 310 323 544 2316 2335 2542 2777 310
Size: 13 221 1772 19 207 235 3514
Fragments arranged by size:
3514 1772 235 221 207 19 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 2293 2818 3157 4036 4750 5048 595
Size: 1698 525 339 879 714 298 1528
Fragments arranged by size:
1698 1528 879 714 525 339 298
BsaI GGTCTCn'nnnn_
Cuts at: 788 2582 5685 788
Size: 1794 3103 1084
Fragments arranged by size:
3103 1794 1084
BsaAI yAC'GTr
Cuts at: 3334 3334
Size: 5981
BsaBI GATnn'nnATC
Cuts at: 6 3495 5532 6
Size: 3489 2037 455
Fragments arranged by size:
3489 2037 455
BsaHI Gr'CG_yC
Cuts at: 125 803 2416 3412 3660 4362 5858 125
Size: 678 1613 996 248 702 1496 248
Fragments arranged by size:
1613 1496 996 702 678 248 248
BsaWI w'CCGG_w
Cuts at: 869 2448 3170 3691 4369 4780 4927 5201 869
Size: 1579 722 521 678 411 147 274 1649
Fragments arranged by size:
1649 1579 722 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2219 2249 2219
Size: 30 5951
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1103 1969 1972 2468 2603 1103
Size: 866 3 496 135 4481
Fragments arranged by size:
4481 866 496 135 3
BseYI C'CCAG_C
Cuts at: 2135 2471 3406 4164 4829 5237 2135
Size: 336 935 758 665 408 2879
Fragments arranged by size:
2879 935 758 665 408 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2058 2507 2058
Size: 449 5532
BsiEI CG_ry'CG
Cuts at: 104 172 2680 3523 3569 4799 104
Size: 68 2508 843 46 1230 1286
Fragments arranged by size:
2508 1286 1230 843 68 46
BsiHKAI G_wGCw'C
Cuts at: 311 644 1072 1482 1849 2995 3773 3963 4823
Size: 333 428 410 367 1146 778 190 860
Cuts at: 4823 311
Size: 1469
Fragments arranged by size:
1469 1146 860 778 428 410 367 333
190
BsmI GAATG_Cn'
Cuts at: 1119 1119
Size: 5981
Bsp1286I G_dGCh'C
Cuts at: 311 563 644 672 1072 1244 1482 1710 1849
Size: 252 81 28 400 172 238 228 139
Cuts at: 1849 1883 1933 2142 2573 2861 2972 2995 3606
Size: 34 50 209 431 288 111 23 611
Cuts at: 3606 3699 3773 3963 4025 4823 311
Size: 93 74 190 62 798 1469
Fragments arranged by size:
1469 798 611 431 400 288 252 238
228 209 190 172 139 111 93 81
74 62 50 34 28 23
BspEI T'CCGG_A
Cuts at: 869 2448 869
Size: 1579 4402
BspHI T'CATG_A
Cuts at: 1901 4413 1901
Size: 2512 3469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1722 2529 2861 3031 3245 3547 3928 1722
Size: 807 332 170 214 302 381 3775
Fragments arranged by size:
3775 807 381 332 302 214 170
BsrBI CCG'CTC
Cuts at: 50 4273 4327 5811 5976 50
Size: 4223 54 1484 165 55
Fragments arranged by size:
4223 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1446 2091 3893 1446
Size: 645 1802 3534
Fragments arranged by size:
3534 1802 645
BsrFI r'CCGG_y
Cuts at: 2621 3979 4160 2621
Size: 1358 181 4442
Fragments arranged by size:
4442 1358 181
BssSI C'ACGA_G
Cuts at: 757 880 1144 4252 4960 757
Size: 123 264 3108 708 1778
Fragments arranged by size:
3108 1778 708 264 123
BstAPI GCAn_nnn'nTGC
Cuts at: 2803 2901 3358 2803
Size: 98 457 5426
Fragments arranged by size:
5426 457 98
BstBI TT'CG_AA
Cuts at: 4342 4342
Size: 5981
BstEII G'GTnAC_C
Cuts at: 409 409
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 1047 1125 1162 1615 1047
Size: 78 37 453 5413
Fragments arranged by size:
5413 453 78 37
BstYI r'GATC_y
Cuts at: 1 598 1832 1916 3006 3473 3496 3831 4077
Size: 597 1234 84 1090 467 23 335 246
Cuts at: 4077 4383 4395 4481 5533 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1234 1090 1052 597 467 449 335 306
246 86 84 23 12
Bsu36I CC'TnA_GG
Cuts at: 1459 1952 1459
Size: 493 5488
BtgI C'CryG_G
Cuts at: 172 456 903 1029 1136 2091 2166 2821 172
Size: 284 447 126 107 955 75 655 3332
Fragments arranged by size:
3332 955 655 447 284 126 107 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3931 4112 4275 3931
Size: 181 163 5637
Fragments arranged by size:
5637 181 163
BtsI GCAGTG_nn'
Cuts at: 444 1555 1587 1687 2041 2121 2527 2752 3224
Size: 1111 32 100 354 80 406 225 472
Cuts at: 3224 444
Size: 3201
Fragments arranged by size:
3201 1111 472 406 354 225 100 80
32
DraI TTT'AAA
Cuts at: 728 2980 728
Size: 2252 3729
DrdI GACnn_nn'nnGTC
Cuts at: 480 2598 3687 5031 480
Size: 2118 1089 1344 1430
Fragments arranged by size:
2118 1430 1344 1089
EaeI y'GGCC_r
Cuts at: 169 1061 1196 1635 2088 2237 2623 2677 3566
Size: 892 135 439 453 149 386 54 889
Cuts at: 3566 3740 4131 4158 5503 169
Size: 174 391 27 1345 647
Fragments arranged by size:
1345 892 889 647 453 439 391 386
174 149 135 54 27
EagI C'GGCC_G
Cuts at: 169 2677 3566 169
Size: 2508 889 2584
Fragments arranged by size:
2584 2508 889
EarI CTCTTCn'nnn_
Cuts at: 677 1388 1409 4004 4214 677
Size: 711 21 2595 210 2444
Fragments arranged by size:
2595 2444 711 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1486 4189 4931 5077 5791 1486
Size: 2703 742 146 714 1676
Fragments arranged by size:
2703 1676 742 714 146
EcoICRI GAG'CTC
Cuts at: 309 1847 2993 309
Size: 1538 1146 3297
Fragments arranged by size:
3297 1538 1146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 370 658 1106 1885 2084 2246 2343 2477
Size: 119 288 448 779 199 162 97 134
Cuts at: 2477 2652 2845 3806 4238 4591 5253 251
Size: 175 193 961 432 353 662 979
Fragments arranged by size:
979 961 779 662 448 432 353 288
199 193 175 162 134 119 97
EcoO109I rG'GnC_Cy
Cuts at: 1523 1699 1948 2139 2212 2513 2569 3120 1523
Size: 176 249 191 73 301 56 551 4384
Fragments arranged by size:
4384 551 301 249 191 176 73 56
EcoRI G'AATT_C
Cuts at: 2985 2985
Size: 5981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4000 4000
Size: 5981
FspI TGC'GCA
Cuts at: 3762 3762
Size: 5981
HaeII r_GCGC'y
Cuts at: 128 3415 3663 4893 5664 128
Size: 3287 248 1230 771 445
Fragments arranged by size:
3287 1230 771 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 323 355 715 747 1177 1209 1824 1856 2196
Size: 32 360 32 430 32 615 32 340
Cuts at: 2196 2228 2430 2462 3823 3853 3855 3885 323
Size: 32 202 32 1361 30 2 30 2419
Fragments arranged by size:
2419 1361 615 430 360 340 202 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1095 3020 1095
Size: 1925 4056
KasI G'GCGC_C
Cuts at: 124 3411 3659 124
Size: 3287 248 2446
Fragments arranged by size:
3287 2446 248
KpnI G_GTAC'C
Cuts at: 1622 2852 3001 1622
Size: 1230 149 4602
Fragments arranged by size:
4602 1230 149
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 943 1349 1895 4740 4924 943
Size: 406 546 2845 184 2000
Fragments arranged by size:
2845 2000 546 406 184
MscI TGG'CCA
Cuts at: 1063 2090 2239 3742 1063
Size: 1027 149 1503 3302
Fragments arranged by size:
3302 1503 1027 149
MslI CAynn'nnrTG
Cuts at: 1018 1063 1123 1342 2245 2266 3970 4097 1018
Size: 45 60 219 903 21 1704 127 2902
Fragments arranged by size:
2902 1704 903 219 127 60 45 21
MspA1I CmG'CkG
Cuts at: 174 1231 1692 2378 2974 3092 3406 3766 4550
Size: 1057 461 686 596 118 314 360 784
Cuts at: 4550 4795 174
Size: 245 1360
Fragments arranged by size:
1360 1057 784 686 596 461 360 314
245 118
NaeI GCC'GGC
Cuts at: 2623 4162 2623
Size: 1539 4442
NarI GG'CG_CC
Cuts at: 125 3412 3660 125
Size: 3287 248 2446
Fragments arranged by size:
3287 2446 248
NcoI C'CATG_G
Cuts at: 1136 2091 1136
Size: 955 5026
NgoMIV G'CCGG_C
Cuts at: 2621 4160 2621
Size: 1539 4442
NspI r_CATG'y
Cuts at: 915 1023 1332 1662 1971 3034 3534 5137 915
Size: 108 309 330 309 1063 500 1603 1759
Fragments arranged by size:
1759 1603 1063 500 330 309 309 108
PasI CC'CwG_GG
Cuts at: 1978 2421 2566 1978
Size: 443 145 5393
Fragments arranged by size:
5393 443 145
PciI A'CATG_T
Cuts at: 911 5133 911
Size: 4222 1759
PflMI CCAn_nnn'nTGG
Cuts at: 665 1149 665
Size: 484 5497
PfoI T'CCnGG_A
Cuts at: 341 1559 2602 341
Size: 1218 1043 3720
Fragments arranged by size:
3720 1218 1043
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 5949
PpuMI rG'GwC_Cy
Cuts at: 1523 1699 1948 2212 2513 1523
Size: 176 249 264 301 4991
Fragments arranged by size:
4991 301 264 249 176
PspOMI G'GGCC_C
Cuts at: 2138 2569 2138
Size: 431 5550
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 233 265 233
Size: 32 5949
PstI C_TGCA'G
Cuts at: 161 236 1185 1301 2284 2414 3028 3713 161
Size: 75 949 116 983 130 614 685 2429
Fragments arranged by size:
2429 983 949 685 614 130 116 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5981
PvuII CAG'CTG
Cuts at: 1231 1692 3406 3766 1231
Size: 461 1714 360 3446
Fragments arranged by size:
3446 1714 461 360
RsrII CG'GwC_CG
Cuts at: 4176 4176
Size: 5981
SacI G_AGCT'C
Cuts at: 311 1849 2995 311
Size: 1538 1146 3297
Fragments arranged by size:
3297 1538 1146
SacII CC_GC'GG
Cuts at: 175 175
Size: 5981
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SanDI GG'GwC_CC
Cuts at: 1523 1699 2513 1523
Size: 176 814 4991
Fragments arranged by size:
4991 814 176
SapI GCTCTTCn'nnn_
Cuts at: 677 4004 4214 677
Size: 3327 210 2444
Fragments arranged by size:
3327 2444 210
SbfI CC_TGCA'GG
Cuts at: 3028 3028
Size: 5981
ScaI AGT'ACT
Cuts at: 5498 5498
Size: 5981
SfcI C'TryA_G
Cuts at: 33 157 232 1181 1297 2280 2410 3024 3709
Size: 124 75 949 116 983 130 614 685
Cuts at: 3709 4677 4868 33
Size: 968 191 1146
Fragments arranged by size:
1146 983 968 949 685 614 191 130
124 116 75
SfoI GGC'GCC
Cuts at: 126 3413 3661 126
Size: 3287 248 2446
Fragments arranged by size:
3287 2446 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SmaI CCC'GGG
Cuts at: 623 2574 3003 623
Size: 1951 429 3601
Fragments arranged by size:
3601 1951 429
SmlI C'TyrA_G
Cuts at: 610 2308 2833 3136 4051 4765 5027 610
Size: 1698 525 303 915 714 262 1564
Fragments arranged by size:
1698 1564 915 714 525 303 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 3034 3534 3034
Size: 500 5481
StuI AGG'CCT
Cuts at: 1642 2610 2673 1642
Size: 968 63 4950
Fragments arranged by size:
4950 968 63
StyI C'CwwG_G
Cuts at: 1136 2091 2941 3115 3469 1136
Size: 955 850 174 354 3648
Fragments arranged by size:
3648 955 850 354 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1205 1948 3564 3697 4156 4365 1205
Size: 743 1616 133 459 209 2821
Fragments arranged by size:
2821 1616 743 459 209 133
TatI w'GTAC_w
Cuts at: 2270 5496 2270
Size: 3226 2755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 932 2219 2251 5498 5530 900
Size: 32 1287 32 3247 32 1351
Fragments arranged by size:
3247 1351 1287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2595 3778 2595
Size: 1183 4798
XbaI T'CTAG_A
Cuts at: 3012 3012
Size: 5981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 741 1044 2247 3476 432
Size: 309 303 1203 1229 2937
Fragments arranged by size:
2937 1229 1203 309 303
XmaI C'CCGG_G
Cuts at: 621 2572 3001 621
Size: 1951 429 3601
Fragments arranged by size:
3601 1951 429
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BfrBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI