Restriction map for
pF1KB7299
(Circular) (Six-base) MAPSORT of: pF1KB7299.seq Check: 6791 from: 1 to: 6785
pF1KB7299 6785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 156 192 3078 156
Size: 36 2886 3863
Fragments arranged by size:
3863 2886 36
AatII G_ACGT'C
Cuts at: 2309 2309
Size: 6785
AccI GT'mk_AC
Cuts at: 1001 3823 1001
Size: 2822 3963
Acc65I G'GTAC_C
Cuts at: 3705 3801 3705
Size: 96 6689
AclI AA'CG_TT
Cuts at: 6625 6625
Size: 6785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2827 3223 4610 5042 5395 6057 2023
Size: 804 396 1387 432 353 662 2751
Fragments arranged by size:
2751 1387 804 662 432 396 353
AfeI AGC'GCT
Cuts at: 943 3244 943
Size: 2301 4484
AflII C'TTAA_G
Cuts at: 1191 1191
Size: 6785
AflIII A'CryG_T
Cuts at: 329 1451 1805 2932 3304 4137 5937 6218 329
Size: 1122 354 1127 372 833 1800 281 896
Fragments arranged by size:
1800 1127 1122 896 833 372 354 281
AleI CACnn'nnGTG
Cuts at: 186 1048 3110 186
Size: 862 2062 3861
Fragments arranged by size:
3861 2062 862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 200 204 2723 200
Size: 4 2519 4262
Fragments arranged by size:
4262 2519 4
AlwNI CAG_nnn'CTG
Cuts at: 217 1910 3180 3273 3567 4090 5528 217
Size: 1693 1270 93 294 523 1438 1474
Fragments arranged by size:
1693 1474 1438 1270 523 294 93
ApaI G_GGCC'C
Cuts at: 3089 3089
Size: 6785
ApaLI G'TGCA_C
Cuts at: 2967 2980 3519 5623 2967
Size: 13 539 2104 4129
Fragments arranged by size:
4129 2104 539 13
ApoI r'AATT_y
Cuts at: 1549 1765 2536 3789 6138 6394 1549
Size: 216 771 1253 2349 256 1940
Fragments arranged by size:
2349 1940 1253 771 256 216
AseI AT'TA_AT
Cuts at: 20 1364 20
Size: 1344 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
AvaI C'yCGr_G
Cuts at: 225 2030 2581 2652 3483 3805 225
Size: 1805 551 71 831 322 3205
Fragments arranged by size:
3205 1805 831 551 322 71
BamHI G'GATC_C
Cuts at: 3810 3810
Size: 6785
BanI G'GyrC_C
Cuts at: 180 1733 1811 2865 2944 3455 3705 3801 4215
Size: 1553 78 1054 79 511 250 96 414
Cuts at: 4215 4463 4498 180
Size: 248 35 2467
Fragments arranged by size:
2467 1553 1054 511 414 250 248 96
79 78 35
BanII G_rGCy'C
Cuts at: 226 740 1301 1990 2421 2582 3089 3209 3799
Size: 514 561 689 431 161 507 120 590
Cuts at: 3799 4829 226
Size: 1030 2182
Fragments arranged by size:
2182 1030 689 590 561 514 507 431
161 120
BbeI G_GCGC'C
Cuts at: 4219 4467 4219
Size: 248 6537
BbsI GAAGACnn'nnnn_
Cuts at: 864 899 1503 1877 2309 864
Size: 35 604 374 432 5340
Fragments arranged by size:
5340 604 432 374 35
BbvCI CC'TCA_GC
Cuts at: 3020 3020
Size: 6785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2535 2569 3540 3574 2535
Size: 34 971 34 5746
Fragments arranged by size:
5746 971 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1625 2437 4683 5739 6785 1625
Size: 812 2246 1056 1046 1625
Fragments arranged by size:
2246 1625 1056 1046 812
BclI T'GATC_A
Cuts at: 1584 4305 1584
Size: 2721 4064
BfrBI ATG'CAT
Cuts at: 3222 3222
Size: 6785
BglI GCCn_nnn'nGGC
Cuts at: 178 3625 178
Size: 3447 3338
BglII A'GATC_T
Cuts at: 4300 6337 4300
Size: 2037 4748
BlpI GC'TnA_GC
Cuts at: 3897 3897
Size: 6785
Bme1580I G_kGCm'C
Cuts at: 377 2507 2548 2949 2971 2984 3019 3089 3523
Size: 2130 41 401 22 13 35 70 434
Cuts at: 3523 4410 4503 5627 377
Size: 887 93 1124 1535
Fragments arranged by size:
2130 1535 1124 887 434 401 93 70
41 35 22 13
BmgBI CAC'GTC
Cuts at: 2933 2933
Size: 6785
BmrI ACTGGGnnnn_n'
Cuts at: 3285 3997 4078 4413 6557 3285
Size: 712 81 335 2144 3513
Fragments arranged by size:
3513 2144 712 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3396 5152 3396
Size: 1756 5029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1887 2435 2934 3018 3436 402
Size: 1485 548 499 84 418 3751
Fragments arranged by size:
3751 1485 548 499 418 84
Bpu10I CC'TnA_GC
Cuts at: 3020 3020
Size: 6785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 1550 2105 3961 4840 5554 5852 759
Size: 791 555 1856 879 714 298 1692
Fragments arranged by size:
1856 1692 879 791 714 555 298
BsaI GGTCTCn'nnnn_
Cuts at: 300 979 2362 2795 3518 6489 300
Size: 679 1383 433 723 2971 596
Fragments arranged by size:
2971 1383 723 679 596 433
BsaAI yAC'GTr
Cuts at: 330 714 1050 4138 330
Size: 384 336 3088 2977
Fragments arranged by size:
3088 2977 384 336
BsaBI GATnn'nnATC
Cuts at: 6 4299 6336 6
Size: 4293 2037 455
Fragments arranged by size:
4293 2037 455
BsaHI Gr'CG_yC
Cuts at: 2306 4216 4464 5166 6662 2306
Size: 1910 248 702 1496 2429
Fragments arranged by size:
2429 1910 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3974 4495 5173 5584 5731 6005 3974
Size: 521 678 411 147 274 4754
Fragments arranged by size:
4754 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 559 589 1907 1937 2620 2650 559
Size: 30 1318 30 683 30 4694
Fragments arranged by size:
4694 1318 683 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 567 233
Size: 334 6451
BseYI C'CCAG_C
Cuts at: 878 2711 2716 2893 3409 3571 3710 4210 4968
Size: 1833 5 177 516 162 139 500 758
Cuts at: 4968 5633 6041 878
Size: 665 408 1622
Fragments arranged by size:
1833 1622 758 665 516 500 408 177
162 139 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 535 790 1341 1929 2325 2983 3180 3580
Size: 33 255 551 588 396 658 197 400
Cuts at: 3580 3586 3744 502
Size: 6 158 3543
Fragments arranged by size:
3543 658 588 551 400 396 255 197
158 33 6
BsiEI CG_ry'CG
Cuts at: 104 262 2529 3583 4327 4373 5603 104
Size: 158 2267 1054 744 46 1230 1286
Fragments arranged by size:
2267 1286 1230 1054 744 158 46
BsiHKAI G_wGCw'C
Cuts at: 1055 2072 2752 2971 2984 3103 3154 3298 3394
Size: 1017 680 219 13 119 51 144 96
Cuts at: 3394 3523 3799 4577 4767 5627 1055
Size: 129 276 778 190 860 2213
Fragments arranged by size:
2213 1017 860 778 680 276 219 190
144 129 119 96 51 13
BsmBI CGTCTCn'nnnn_
Cuts at: 2454 2454
Size: 6785
Bsp1286I G_dGCh'C
Cuts at: 226 377 740 1055 1301 1990 2072 2421 2507
Size: 151 363 315 246 689 82 349 86
Cuts at: 2507 2548 2582 2752 2949 2971 2984 3019 3089
Size: 41 34 170 197 22 13 35 70
Cuts at: 3089 3103 3154 3209 3298 3394 3523 3799 4410
Size: 14 51 55 89 96 129 276 611
Cuts at: 4410 4503 4577 4767 4829 5627 226
Size: 93 74 190 62 798 1384
Fragments arranged by size:
1384 798 689 611 363 349 315 276
246 197 190 170 151 129 96 93
89 86 82 74 70 62 55 51
41 35 34 22 14 13
BspHI T'CATG_A
Cuts at: 1245 1434 5217 1245
Size: 189 3783 2813
Fragments arranged by size:
3783 2813 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 192 604 1918 3078 3835 4049 4351 4732
Size: 36 412 1314 1160 757 214 302 381
Cuts at: 4732 156
Size: 2209
Fragments arranged by size:
2209 1314 1160 757 412 381 302 214
36
BsrBI CCG'CTC
Cuts at: 50 258 1564 5077 5131 6615 6780 50
Size: 208 1306 3513 54 1484 165 55
Fragments arranged by size:
3513 1484 1306 208 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1115 1397 1509 2481 3650 4697 1115
Size: 282 112 972 1169 1047 3203
Fragments arranged by size:
3203 1169 1047 972 282 112
BsrFI r'CCGG_y
Cuts at: 2528 3578 3582 4783 4964 2528
Size: 1050 4 1201 181 4349
Fragments arranged by size:
4349 1201 1050 181 4
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 6785
BssHII G'CGCG_C
Cuts at: 119 121 119
Size: 2 6783
BssSI C'ACGA_G
Cuts at: 5056 5764 5056
Size: 708 6077
BstAPI GCAn_nnn'nTGC
Cuts at: 3180 3749 3761 4162 3180
Size: 569 12 401 5803
Fragments arranged by size:
5803 569 401 12
BstBI TT'CG_AA
Cuts at: 5146 5146
Size: 6785
BstEII G'GTnAC_C
Cuts at: 768 1520 2400 768
Size: 752 880 5153
Fragments arranged by size:
5153 880 752
BstXI CCAn_nnnn'nTGG
Cuts at: 748 1411 1744 3074 748
Size: 663 333 1330 4459
Fragments arranged by size:
4459 1330 663 333
BstYI r'GATC_y
Cuts at: 1 438 727 2287 3810 4277 4300 4635 4881
Size: 437 289 1560 1523 467 23 335 246
Cuts at: 4881 5187 5199 5285 6337 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1560 1523 1052 467 449 437 335 306
289 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 646 3588 646
Size: 2942 3843
BtgI C'CryG_G
Cuts at: 194 251 1023 1863 2513 2903 194
Size: 57 772 840 650 390 4076
Fragments arranged by size:
4076 840 772 650 390 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1946 4735 4916 5079 1946
Size: 2789 181 163 3652
Fragments arranged by size:
3652 2789 181 163
BtsI GCAGTG_nn'
Cuts at: 377 2971 3143 3274 3568 3754 4028 377
Size: 2594 172 131 294 186 274 3134
Fragments arranged by size:
3134 2594 294 274 186 172 131
DraI TTT'AAA
Cuts at: 1707 3784 1707
Size: 2077 4708
DraIII CAC_nnn'GTG
Cuts at: 1658 1658
Size: 6785
DrdI GACnn_nn'nnGTC
Cuts at: 4491 5835 4491
Size: 1344 5441
EaeI y'GGCC_r
Cuts at: 170 259 1026 1866 2526 3005 3164 3524 3580
Size: 89 767 840 660 479 159 360 56
Cuts at: 3580 4370 4544 4935 4962 6307 170
Size: 790 174 391 27 1345 648
Fragments arranged by size:
1345 840 790 767 660 648 479 391
360 174 159 89 56 27
EagI C'GGCC_G
Cuts at: 259 2526 3580 4370 259
Size: 2267 1054 790 2674
Fragments arranged by size:
2674 2267 1054 790
EarI CTCTTCn'nnn_
Cuts at: 360 401 623 904 953 4808 5018 360
Size: 41 222 281 49 3855 210 2127
Fragments arranged by size:
3855 2127 281 222 210 49 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 375 3270 4993 5735 5881 6595 375
Size: 2895 1723 742 146 714 565
Fragments arranged by size:
2895 1723 742 714 565 146
EcoICRI GAG'CTC
Cuts at: 3797 3797
Size: 6785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1887 2023 2435 2827 2934 3018 3223 3436
Size: 1485 136 412 392 107 84 205 213
Cuts at: 3436 4610 5042 5395 6057 402
Size: 1174 432 353 662 1130
Fragments arranged by size:
1485 1174 1130 662 432 412 392 353
213 205 136 107 84
EcoNI CCTnn'n_nnAGG
Cuts at: 3036 3036
Size: 6785
EcoO109I rG'GnC_Cy
Cuts at: 199 762 956 1849 1907 3086 3478 3924 199
Size: 563 194 893 58 1179 392 446 3060
Fragments arranged by size:
3060 1179 893 563 446 392 194 58
EcoRI G'AATT_C
Cuts at: 3789 3789
Size: 6785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 945 3792 4804 945
Size: 2847 1012 2926
Fragments arranged by size:
2926 2847 1012
FseI GG_CCGG'CC
Cuts at: 3582 3586 3582
Size: 4 6781
FspI TGC'GCA
Cuts at: 2473 4566 2473
Size: 2093 4692
HaeII r_GCGC'y
Cuts at: 148 217 945 3083 3246 4219 4467 5697 6468
Size: 69 728 2138 163 973 248 1230 771
Cuts at: 6468 148
Size: 465
Fragments arranged by size:
2138 1230 973 771 728 465 248 163
69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 316 348 376 408 907 939 1094
Size: 32 4 32 28 32 499 32 155
Cuts at: 1094 1126 4627 4657 4659 4689 280
Size: 32 3501 30 2 30 2376
Fragments arranged by size:
3501 2376 499 155 32 32 32 32
32 30 30 28 4 2
HincII GTy'rAC
Cuts at: 312 1002 2043 3824 312
Size: 690 1041 1781 3273
Fragments arranged by size:
3273 1781 1041 690
KasI G'GCGC_C
Cuts at: 4215 4463 4215
Size: 248 6537
KpnI G_GTAC'C
Cuts at: 3709 3805 3709
Size: 96 6689
MfeI C'AATT_G
Cuts at: 1121 1121
Size: 6785
MluI A'CGCG_T
Cuts at: 6218 6218
Size: 6785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1856 2247 2908 3797 5544 5728 1856
Size: 391 661 889 1747 184 2913
Fragments arranged by size:
2913 1747 889 661 391 184
MscI TGG'CCA
Cuts at: 1028 1868 4546 1028
Size: 840 2678 3267
Fragments arranged by size:
3267 2678 840
MslI CAynn'nnrTG
Cuts at: 186 851 965 1028 1048 2467 3072 3110 4774
Size: 665 114 63 20 1419 605 38 1664
Cuts at: 4774 4901 186
Size: 127 2070
Fragments arranged by size:
2070 1664 1419 665 605 127 114 63
38 20
MspA1I CmG'CkG
Cuts at: 208 253 357 1355 2897 3177 3494 3530 3896
Size: 45 104 998 1542 280 317 36 366
Cuts at: 3896 4210 4570 5354 5599 208
Size: 314 360 784 245 1394
Fragments arranged by size:
1542 1394 998 784 366 360 317 314
280 245 104 45 36
NaeI GCC'GGC
Cuts at: 3580 3584 4966 3580
Size: 4 1382 5399
Fragments arranged by size:
5399 1382 4
NarI GG'CG_CC
Cuts at: 4216 4464 4216
Size: 248 6537
NcoI C'CATG_G
Cuts at: 194 1023 1863 194
Size: 829 840 5116
Fragments arranged by size:
5116 840 829
NgoMIV G'CCGG_C
Cuts at: 3578 3582 4964 3578
Size: 4 1382 5399
Fragments arranged by size:
5399 1382 4
NsiI A_TGCA'T
Cuts at: 3224 3224
Size: 6785
NspI r_CATG'y
Cuts at: 876 1455 1698 1809 3308 3838 4338 5941 876
Size: 579 243 111 1499 530 500 1603 1720
Fragments arranged by size:
1720 1603 1499 579 530 500 243 111
PasI CC'CwG_GG
Cuts at: 3089 3089
Size: 6785
PciI A'CATG_T
Cuts at: 1451 1805 3304 5937 1451
Size: 354 1499 2633 2299
Fragments arranged by size:
2633 2299 1499 354
PflMI CCAn_nnn'nTGG
Cuts at: 1966 1966
Size: 6785
PfoI T'CCnGG_A
Cuts at: 1285 1903 1285
Size: 618 6167
PmeI GTTT'AAAC
Cuts at: 3784 3784
Size: 6785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 5231
Size: 32 6753
PpuMI rG'GwC_Cy
Cuts at: 199 762 956 1907 3478 199
Size: 563 194 951 1571 3506
Fragments arranged by size:
3506 1571 951 563 194
PspOMI G'GGCC_C
Cuts at: 3085 3085
Size: 6785
PspXI vC'TCGA_Gb
Cuts at: 3483 3483
Size: 6785
PstI C_TGCA'G
Cuts at: 1531 2926 3269 3832 4517 1531
Size: 1395 343 563 685 3799
Fragments arranged by size:
3799 1395 685 563 343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6785
PvuII CAG'CTG
Cuts at: 1355 2897 3177 3494 3530 4210 4570 1355
Size: 1542 280 317 36 680 360 3570
Fragments arranged by size:
3570 1542 680 360 317 280 36
RsrII CG'GwC_CG
Cuts at: 4980 4980
Size: 6785
SacI G_AGCT'C
Cuts at: 3799 3799
Size: 6785
SacII CC_GC'GG
Cuts at: 254 254
Size: 6785
SalI G'TCGA_C
Cuts at: 1000 3822 1000
Size: 2822 3963
SanDI GG'GwC_CC
Cuts at: 199 3478 199
Size: 3279 3506
SapI GCTCTTCn'nnn_
Cuts at: 360 4808 5018 360
Size: 4448 210 2127
Fragments arranged by size:
4448 2127 210
SbfI CC_TGCA'GG
Cuts at: 3832 3832
Size: 6785
ScaI AGT'ACT
Cuts at: 6302 6302
Size: 6785
SexAI A'CCwGG_T
Cuts at: 290 3701 290
Size: 3411 3374
SfcI C'TryA_G
Cuts at: 33 1527 2559 2796 2922 3265 3828 4513 5481
Size: 1494 1032 237 126 343 563 685 968
Cuts at: 5481 5672 33
Size: 191 1146
Fragments arranged by size:
1494 1146 1032 968 685 563 343 237
191 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 3625 3625
Size: 6785
SfoI GGC'GCC
Cuts at: 4217 4465 4217
Size: 248 6537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
SmaI CCC'GGG
Cuts at: 2032 2654 3807 2032
Size: 622 1153 5010
Fragments arranged by size:
5010 1153 622
SmlI C'TyrA_G
Cuts at: 774 1191 1565 2120 3483 3940 4855 5569 5831
Size: 417 374 555 1363 457 915 714 262
Cuts at: 5831 774
Size: 1728
Fragments arranged by size:
1728 1363 915 714 555 457 417 374
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6785
SphI G_CATG'C
Cuts at: 1698 3838 4338 1698
Size: 2140 500 4145
Fragments arranged by size:
4145 2140 500
StuI AGG'CCT
Cuts at: 3046 3046
Size: 6785
StyI C'CwwG_G
Cuts at: 194 600 693 887 989 1023 1863 2283 3919
Size: 406 93 194 102 34 840 420 1636
Cuts at: 3919 4273 194
Size: 354 2706
Fragments arranged by size:
2706 1636 840 420 406 354 194 102
93 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1876 2707 4368 4501 4960 5169 1338
Size: 538 831 1661 133 459 209 2954
Fragments arranged by size:
2954 1661 831 538 459 209 133
TatI w'GTAC_w
Cuts at: 719 6300 719
Size: 5581 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 559 591 1814 1846 1965 1997 2640 2672 2901
Size: 32 1223 32 119 32 643 32 229
Cuts at: 2901 2933 6302 6334 559
Size: 32 3369 32 1010
Fragments arranged by size:
3369 1223 1010 643 229 119 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 292 2278 3190 3552 4582 292
Size: 1986 912 362 1030 2495
Fragments arranged by size:
2495 1986 1030 912 362
XbaI T'CTAG_A
Cuts at: 1140 3816 1140
Size: 2676 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 762 1213 2682 2901 3417 4280 459
Size: 303 451 1469 219 516 863 2964
Fragments arranged by size:
2964 1469 863 516 451 303 219
XhoI C'TCGA_G
Cuts at: 3483 3483
Size: 6785
XmaI C'CCGG_G
Cuts at: 2030 2652 3805 2030
Size: 622 1153 5010
Fragments arranged by size:
5010 1153 622
XmnI GAAnn'nnTTC
Cuts at: 3328 3328
Size: 6785
ZraI GAC'GTC
Cuts at: 2307 2307
Size: 6785
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AloI AscI AvrII BaeI BmtI BsiWI
BsmI BspEI BstZ17I ClaI CspCI EcoRV FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PmlI PshAI
PsiI PsrI SgrAI SnaBI SrfI SspI SwaI