Restriction map for
pF1KB7297
(Circular) (Six-base) MAPSORT of: pF1KB7297.seq Check: 8149 from: 1 to: 6791
pF1KB7297 6791 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1098 2317 3081 1098
Size: 1219 764 4808
Fragments arranged by size:
4808 1219 764
AccI GT'mk_AC
Cuts at: 3829 3829
Size: 6791
Acc65I G'GTAC_C
Cuts at: 3807 3807
Size: 6791
AclI AA'CG_TT
Cuts at: 6631 6631
Size: 6791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2546 4616 5048 5401 6063 2546
Size: 2070 432 353 662 3274
Fragments arranged by size:
3274 2070 662 432 353
AflIII A'CryG_T
Cuts at: 717 4143 5943 6224 717
Size: 3426 1800 281 1284
Fragments arranged by size:
3426 1800 1284 281
AleI CACnn'nnGTG
Cuts at: 2777 2777
Size: 6791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 391 910 1238 2018 2653 3629 391
Size: 519 328 780 635 976 3553
Fragments arranged by size:
3553 976 780 635 519 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2077 2109 2077
Size: 32 6759
AlwNI CAG_nnn'CTG
Cuts at: 298 617 763 1065 2153 2922 3151 3433 3505
Size: 319 146 302 1088 769 229 282 72
Cuts at: 3505 3712 4096 5534 298
Size: 207 384 1438 1555
Fragments arranged by size:
1555 1438 1088 769 384 319 302 282
229 207 146 72
ApaI G_GGCC'C
Cuts at: 1205 1682 1898 1962 2519 2688 3415 1205
Size: 477 216 64 557 169 727 4581
Fragments arranged by size:
4581 727 557 477 216 169 64
ApaLI G'TGCA_C
Cuts at: 1703 1993 5629 1703
Size: 290 3636 2865
Fragments arranged by size:
3636 2865 290
ApoI r'AATT_y
Cuts at: 3795 6144 6400 3795
Size: 2349 256 4186
Fragments arranged by size:
4186 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6791
AvaI C'yCGr_G
Cuts at: 3121 3408 3667 3811 3121
Size: 287 259 144 6101
Fragments arranged by size:
6101 287 259 144
BamHI G'GATC_C
Cuts at: 499 2714 3816 499
Size: 2215 1102 3474
Fragments arranged by size:
3474 2215 1102
BanI G'GyrC_C
Cuts at: 413 1392 2019 2329 2866 3249 3754 3807 4221
Size: 979 627 310 537 383 505 53 414
Cuts at: 4221 4469 4504 413
Size: 248 35 2700
Fragments arranged by size:
2700 979 627 537 505 414 383 310
248 53 35
BanII G_rGCy'C
Cuts at: 1205 1454 1619 1682 1786 1898 1962 2304 2519
Size: 249 165 63 104 112 64 342 215
Cuts at: 2519 2688 3302 3415 3579 3805 4835 1205
Size: 169 614 113 164 226 1030 3161
Fragments arranged by size:
3161 1030 614 342 249 226 215 169
165 164 113 112 104 64 63
BbeI G_GCGC'C
Cuts at: 417 1396 4225 4473 417
Size: 979 2829 248 2735
Fragments arranged by size:
2829 2735 979 248
BbsI GAAGACnn'nnnn_
Cuts at: 3315 3743 3315
Size: 428 6363
BbvCI CC'TCA_GC
Cuts at: 1290 1290
Size: 6791
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1096 1130 2999 3033 1096
Size: 34 1869 34 4854
Fragments arranged by size:
4854 1869 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2906 3576 4689 5745 6791 2906
Size: 670 1113 1056 1046 2906
Fragments arranged by size:
2906 1113 1056 1046 670
BclI T'GATC_A
Cuts at: 1040 4311 1040
Size: 3271 3520
BglI GCCn_nnn'nGGC
Cuts at: 552 2542 3240 552
Size: 1990 698 4103
Fragments arranged by size:
4103 1990 698
BglII A'GATC_T
Cuts at: 4306 6343 4306
Size: 2037 4754
BlpI GC'TnA_GC
Cuts at: 1157 1453 3903 1157
Size: 296 2450 4045
Fragments arranged by size:
4045 2450 296
Bme1580I G_kGCm'C
Cuts at: 236 1125 1205 1266 1682 1707 1898 1936 1962
Size: 889 80 61 416 25 191 38 26
Cuts at: 1962 1997 2024 2519 2688 3017 3415 4416 4509
Size: 35 27 495 169 329 398 1001 93
Cuts at: 4509 5633 236
Size: 1124 1394
Fragments arranged by size:
1394 1124 1001 889 495 416 398 329
191 169 93 80 61 38 35 27
26 25
BmgBI CAC'GTC
Cuts at: 2088 2088
Size: 6791
BmrI ACTGGGnnnn_n'
Cuts at: 3021 3045 4003 4084 4419 6563 3021
Size: 24 958 81 335 2144 3249
Fragments arranged by size:
3249 2144 958 335 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5158 5158
Size: 6791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 593 697 1123 1468 1800 2377 2485 2665
Size: 263 104 426 345 332 577 108 180
Cuts at: 2665 3656 3666 3752 330
Size: 991 10 86 3369
Fragments arranged by size:
3369 991 577 426 345 332 263 180
108 104 86 10
Bpu10I CC'TnA_GC
Cuts at: 1066 1290 1890 1066
Size: 224 600 5967
Fragments arranged by size:
5967 600 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2290 2488 2659 3967 4846 5560 5858 2290
Size: 198 171 1308 879 714 298 3223
Fragments arranged by size:
3223 1308 879 714 298 198 171
BsaI GGTCTCn'nnnn_
Cuts at: 794 1306 1363 2744 6495 794
Size: 512 57 1381 3751 1090
Fragments arranged by size:
3751 1381 1090 512 57
BsaAI yAC'GTr
Cuts at: 273 1323 4144 273
Size: 1050 2821 2920
Fragments arranged by size:
2920 2821 1050
BsaBI GATnn'nnATC
Cuts at: 6 4305 6342 6
Size: 4299 2037 455
Fragments arranged by size:
4299 2037 455
BsaHI Gr'CG_yC
Cuts at: 414 1393 2042 3232 4222 4470 5172 6668 414
Size: 979 649 1190 990 248 702 1496 537
Fragments arranged by size:
1496 1190 990 979 702 649 537 248
BsaWI w'CCGG_w
Cuts at: 3980 4501 5179 5590 5737 6011 3980
Size: 521 678 411 147 274 4760
Fragments arranged by size:
4760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 550 580 1096 1126 1453 1483 1846 1876 2185
Size: 30 516 30 327 30 363 30 309
Cuts at: 2185 2215 3181 3211 550
Size: 30 966 30 4130
Fragments arranged by size:
4130 966 516 363 327 309 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1269 1278 1474 1942 2662 2723 2872 1269
Size: 9 196 468 720 61 149 5188
Fragments arranged by size:
5188 720 468 196 149 61 9
BseYI C'CCAG_C
Cuts at: 285 816 837 1118 1130 1147 1514 1866 1961
Size: 531 21 281 12 17 367 352 95
Cuts at: 1961 2015 2274 2322 2681 2892 3021 3036 3602
Size: 54 259 48 359 211 129 15 566
Cuts at: 3602 4216 4974 5639 6047 285
Size: 614 758 665 408 1029
Fragments arranged by size:
1029 758 665 614 566 531 408 367
359 352 281 259 211 129 95 54
48 21 17 15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 361 442 760 967 1382 1423 1656 2335
Size: 183 81 318 207 415 41 233 679
Cuts at: 2335 2785 2920 3120 3288 178
Size: 450 135 200 168 3681
Fragments arranged by size:
3681 679 450 415 318 233 207 200
183 168 135 81 41
BsiEI CG_ry'CG
Cuts at: 104 3348 4333 4379 5609 104
Size: 3244 985 46 1230 1286
Fragments arranged by size:
3244 1286 1230 985 46
BsiHKAI G_wGCw'C
Cuts at: 1163 1328 1454 1707 1786 1997 2304 2642 2836
Size: 165 126 253 79 211 307 338 194
Cuts at: 2836 3058 3344 3579 3805 4583 4773 5633 1163
Size: 222 286 235 226 778 190 860 2321
Fragments arranged by size:
2321 860 778 338 307 286 253 235
226 222 211 194 190 165 126 79
BsmI GAATG_Cn'
Cuts at: 593 1600 2007 593
Size: 1007 407 5377
Fragments arranged by size:
5377 1007 407
Bsp1286I G_dGCh'C
Cuts at: 236 1125 1163 1205 1266 1328 1454 1619 1682
Size: 889 38 42 61 62 126 165 63
Cuts at: 1682 1707 1786 1898 1936 1962 1997 2024 2304
Size: 25 79 112 38 26 35 27 280
Cuts at: 2304 2519 2642 2688 2836 3017 3058 3302 3344
Size: 215 123 46 148 181 41 244 42
Cuts at: 3344 3415 3579 3805 4416 4509 4583 4773 4835
Size: 71 164 226 611 93 74 190 62
Cuts at: 4835 5633 236
Size: 798 1394
Fragments arranged by size:
1394 889 798 611 280 244 226 215
190 181 165 164 148 126 123 112
93 79 74 71 63 62 62 61
46 42 42 41 38 38 35 27
26 25
BspHI T'CATG_A
Cuts at: 968 1308 2096 5223 968
Size: 340 788 3127 2536
Fragments arranged by size:
3127 2536 788 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 146 782 821 1098 1600 2317 2618 3081 3841
Size: 636 39 277 502 717 301 463 760
Cuts at: 3841 4055 4357 4738 146
Size: 214 302 381 2199
Fragments arranged by size:
2199 760 717 636 502 463 381 302
301 277 214 39
BsrBI CCG'CTC
Cuts at: 50 681 852 5083 5137 6621 6786 50
Size: 631 171 4231 54 1484 165 55
Fragments arranged by size:
4231 1484 631 171 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2531 3126 4703 2531
Size: 595 1577 4619
Fragments arranged by size:
4619 1577 595
BsrFI r'CCGG_y
Cuts at: 1315 2398 2738 2810 2958 2981 3374 4789 4970
Size: 1083 340 72 148 23 393 1415 181
Cuts at: 4970 1315
Size: 3136
Fragments arranged by size:
3136 1415 1083 393 340 181 148 72
23
BsrGI T'GTAC_A
Cuts at: 566 566
Size: 6791
BssHII G'CGCG_C
Cuts at: 122 122
Size: 6791
BssSI C'ACGA_G
Cuts at: 5062 5770 5062
Size: 708 6083
BstAPI GCAn_nnn'nTGC
Cuts at: 298 604 1718 3249 3505 4168 298
Size: 306 1114 1531 256 663 2921
Fragments arranged by size:
2921 1531 1114 663 306 256
BstBI TT'CG_AA
Cuts at: 5152 5152
Size: 6791
BstEII G'GTnAC_C
Cuts at: 2394 2394
Size: 6791
BstXI CCAn_nnnn'nTGG
Cuts at: 816 1279 2463 3029 3550 816
Size: 463 1184 566 521 4057
Fragments arranged by size:
4057 1184 566 521 463
BstYI r'GATC_y
Cuts at: 1 499 2318 2714 3816 4283 4306 4641 4887
Size: 498 1819 396 1102 467 23 335 246
Cuts at: 4887 5193 5205 5291 6343 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1819 1102 1052 498 467 449 396 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1206 1252 2987 3713 1206
Size: 46 1735 726 4284
Fragments arranged by size:
4284 1735 726 46
BtgI C'CryG_G
Cuts at: 107 170 930 2456 3200 107
Size: 63 760 1526 744 3698
Fragments arranged by size:
3698 1526 760 744 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4741 4922 5085 4741
Size: 181 163 6447
Fragments arranged by size:
6447 181 163
BtsI GCAGTG_nn'
Cuts at: 1564 1584 1731 1972 2250 3250 3445 4034 1564
Size: 20 147 241 278 1000 195 589 4321
Fragments arranged by size:
4321 1000 589 278 241 195 147 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2685 2720 2685
Size: 35 6756
DraI TTT'AAA
Cuts at: 3790 3790
Size: 6791
DraIII CAC_nnn'GTG
Cuts at: 276 1176 2763 276
Size: 900 1587 4304
Fragments arranged by size:
4304 1587 900
DrdI GACnn_nn'nnGTC
Cuts at: 347 3002 3319 4497 5841 347
Size: 2655 317 1178 1344 1297
Fragments arranged by size:
2655 1344 1297 1178 317
EaeI y'GGCC_r
Cuts at: 1317 1764 2960 3552 4376 4550 4941 4968 6313
Size: 447 1196 592 824 174 391 27 1345
Cuts at: 6313 1317
Size: 1795
Fragments arranged by size:
1795 1345 1196 824 592 447 391 174
27
EagI C'GGCC_G
Cuts at: 4376 4376
Size: 6791
EarI CTCTTCn'nnn_
Cuts at: 2785 4814 5024 2785
Size: 2029 210 4552
Fragments arranged by size:
4552 2029 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 2227 2797 4999 5741 5887 6601 2089
Size: 138 570 2202 742 146 714 2279
Fragments arranged by size:
2279 2202 742 714 570 146 138
EcoICRI GAG'CTC
Cuts at: 1452 1784 2302 3577 3803 1452
Size: 332 518 1275 226 4440
Fragments arranged by size:
4440 1275 518 332 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 593 697 1123 1468 1800 2377 2485 2546
Size: 263 104 426 345 332 577 108 61
Cuts at: 2546 2665 3656 3666 3752 4616 5048 5401 6063
Size: 119 991 10 86 864 432 353 662
Cuts at: 6063 330
Size: 1058
Fragments arranged by size:
1058 991 864 662 577 432 426 353
345 332 263 119 108 104 86 61
10
EcoNI CCTnn'n_nnAGG
Cuts at: 1105 1402 1105
Size: 297 6494
EcoO109I rG'GnC_Cy
Cuts at: 389 771 1143 1202 1607 1678 1679 1894 1940
Size: 382 372 59 405 71 1 215 46
Cuts at: 1940 2139 2516 2607 2685 3412 3484 3492 3599
Size: 199 377 91 78 727 72 8 107
Cuts at: 3599 3930 389
Size: 331 3250
Fragments arranged by size:
3250 727 405 382 377 372 331 215
199 107 91 78 72 71 59 46
8 1
EcoRI G'AATT_C
Cuts at: 3795 3795
Size: 6791
EcoRV GAT'ATC
Cuts at: 3287 3287
Size: 6791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1737 4810 1737
Size: 3073 3718
FspI TGC'GCA
Cuts at: 4572 4572
Size: 6791
HaeII r_GCGC'y
Cuts at: 417 1140 1396 2674 2817 4225 4473 5703 6474
Size: 723 256 1278 143 1408 248 1230 771
Cuts at: 6474 417
Size: 734
Fragments arranged by size:
1408 1278 1230 771 734 723 256 248
143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 280 1270 1302 2185 2217 2699 2731 3062
Size: 32 990 32 883 32 482 32 331
Cuts at: 3062 3094 3574 3591 3606 3623 4633 4663 4665
Size: 32 480 17 15 17 1010 30 2
Cuts at: 4665 4695 248
Size: 30 2344
Fragments arranged by size:
2344 1010 990 883 482 480 331 32
32 32 32 32 30 30 17 17
15 2
HincII GTy'rAC
Cuts at: 3830 3830
Size: 6791
KasI G'GCGC_C
Cuts at: 413 1392 4221 4469 413
Size: 979 2829 248 2735
Fragments arranged by size:
2829 2735 979 248
KpnI G_GTAC'C
Cuts at: 3811 3811
Size: 6791
MluI A'CGCG_T
Cuts at: 6224 6224
Size: 6791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5550 5734 5550
Size: 184 6607
MscI TGG'CCA
Cuts at: 1766 4552 1766
Size: 2786 4005
MslI CAynn'nnrTG
Cuts at: 313 586 892 967 1237 1277 2000 2380 2455
Size: 273 306 75 270 40 723 380 75
Cuts at: 2455 2777 3548 4780 4907 313
Size: 322 771 1232 127 2197
Fragments arranged by size:
2197 1232 771 723 380 322 306 273
270 127 75 75 40
MspA1I CmG'CkG
Cuts at: 172 601 707 837 1436 1629 1686 2015 2513
Size: 429 106 130 599 193 57 329 498
Cuts at: 2513 3087 3902 4216 4576 5360 5605 172
Size: 574 815 314 360 784 245 1358
Fragments arranged by size:
1358 815 784 599 574 498 429 360
329 314 245 193 130 106 57
NaeI GCC'GGC
Cuts at: 2740 2812 2960 4972 2740
Size: 72 148 2012 4559
Fragments arranged by size:
4559 2012 148 72
NarI GG'CG_CC
Cuts at: 414 1393 4222 4470 414
Size: 979 2829 248 2735
Fragments arranged by size:
2829 2735 979 248
NcoI C'CATG_G
Cuts at: 107 930 2456 107
Size: 823 1526 4442
Fragments arranged by size:
4442 1526 823
NdeI CA'TA_TG
Cuts at: 1761 1761
Size: 6791
NgoMIV G'CCGG_C
Cuts at: 2738 2810 2958 4970 2738
Size: 72 148 2012 4559
Fragments arranged by size:
4559 2012 148 72
NspI r_CATG'y
Cuts at: 721 868 946 1242 3844 4344 5947 721
Size: 147 78 296 2602 500 1603 1565
Fragments arranged by size:
2602 1603 1565 500 296 147 78
PasI CC'CwG_GG
Cuts at: 1005 1936 3595 1005
Size: 931 1659 4201
Fragments arranged by size:
4201 1659 931
PciI A'CATG_T
Cuts at: 717 5943 717
Size: 5226 1565
PflMI CCAn_nnn'nTGG
Cuts at: 1873 2760 3433 3634 1873
Size: 887 673 201 5030
Fragments arranged by size:
5030 887 673 201
PfoI T'CCnGG_A
Cuts at: 1786 2642 3465 3516 3643 1786
Size: 856 823 51 127 4934
Fragments arranged by size:
4934 856 823 127 51
PmeI GTTT'AAAC
Cuts at: 3790 3790
Size: 6791
PmlI CAC'GTG
Cuts at: 273 1323 273
Size: 1050 5741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5237 5269 5237
Size: 32 6759
PpuMI rG'GwC_Cy
Cuts at: 389 771 1143 1607 1940 2139 2607 3484 3492
Size: 382 372 464 333 199 468 877 8
Cuts at: 3492 3599 389
Size: 107 3581
Fragments arranged by size:
3581 877 468 464 382 372 333 199
107 8
PspOMI G'GGCC_C
Cuts at: 1201 1678 1894 1958 2515 2684 3411 1201
Size: 477 216 64 557 169 727 4581
Fragments arranged by size:
4581 727 557 477 216 169 64
PstI C_TGCA'G
Cuts at: 241 303 1441 2131 2257 2695 2875 3166 3501
Size: 62 1138 690 126 438 180 291 335
Cuts at: 3501 3510 3838 4523 241
Size: 9 328 685 2509
Fragments arranged by size:
2509 1138 690 685 438 335 328 291
180 126 62 9
PvuI CG_AT'CG
Cuts at: 104 3348 104
Size: 3244 3547
PvuII CAG'CTG
Cuts at: 601 837 1629 2015 4216 4576 601
Size: 236 792 386 2201 360 2816
Fragments arranged by size:
2816 2201 792 386 360 236
RsrII CG'GwC_CG
Cuts at: 4986 4986
Size: 6791
SacI G_AGCT'C
Cuts at: 1454 1786 2304 3579 3805 1454
Size: 332 518 1275 226 4440
Fragments arranged by size:
4440 1275 518 332 226
SacII CC_GC'GG
Cuts at: 173 173
Size: 6791
SalI G'TCGA_C
Cuts at: 3828 3828
Size: 6791
SanDI GG'GwC_CC
Cuts at: 389 2139 3599 389
Size: 1750 1460 3581
Fragments arranged by size:
3581 1750 1460
SapI GCTCTTCn'nnn_
Cuts at: 4814 5024 4814
Size: 210 6581
SbfI CC_TGCA'GG
Cuts at: 3838 3838
Size: 6791
ScaI AGT'ACT
Cuts at: 6308 6308
Size: 6791
SexAI A'CCwGG_T
Cuts at: 1910 2102 2390 1910
Size: 192 288 6311
Fragments arranged by size:
6311 288 192
SfcI C'TryA_G
Cuts at: 33 237 299 882 1437 2127 2253 2691 2871
Size: 204 62 583 555 690 126 438 180
Cuts at: 2871 3162 3497 3506 3834 4519 5487 5678 33
Size: 291 335 9 328 685 968 191 1146
Fragments arranged by size:
1146 968 690 685 583 555 438 335
328 291 204 191 180 126 62 9
SfoI GGC'GCC
Cuts at: 415 1394 4223 4471 415
Size: 979 2829 248 2735
Fragments arranged by size:
2829 2735 979 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6791
SgrAI Cr'CCGG_yG
Cuts at: 2810 2810
Size: 6791
SmaI CCC'GGG
Cuts at: 3669 3813 3669
Size: 144 6647
SmlI C'TyrA_G
Cuts at: 2305 2503 2674 3946 4861 5575 5837 2305
Size: 198 171 1272 915 714 262 3259
Fragments arranged by size:
3259 1272 915 714 262 198 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6791
SphI G_CATG'C
Cuts at: 868 3844 4344 868
Size: 2976 500 3315
Fragments arranged by size:
3315 2976 500
SspI AAT'ATT
Cuts at: 1023 1023
Size: 6791
StuI AGG'CCT
Cuts at: 2284 2701 3406 2284
Size: 417 705 5669
Fragments arranged by size:
5669 705 417
StyI C'CwwG_G
Cuts at: 107 930 2420 2456 2492 3679 3925 4279 107
Size: 823 1490 36 36 1187 246 354 2619
Fragments arranged by size:
2619 1490 1187 823 354 246 36 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 395 2007 2838 3237 4374 4507 4966 5175
Size: 53 1612 831 399 1137 133 459 209
Cuts at: 5175 342
Size: 1958
Fragments arranged by size:
1958 1612 1137 831 459 399 209 133
53
TatI w'GTAC_w
Cuts at: 566 6306 566
Size: 5740 1051
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 985 1017 1096 1128 1344 1376 1543
Size: 32 462 32 79 32 216 32 167
Cuts at: 1543 1575 1686 1718 2206 2238 3181 3213 6308
Size: 32 111 32 488 32 943 32 3095
Cuts at: 6308 6340 491
Size: 32 942
Fragments arranged by size:
3095 943 942 488 462 216 167 111
79 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 4588 3151
Size: 1437 5354
XbaI T'CTAG_A
Cuts at: 3822 3822
Size: 6791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 277 483 2538 3242 4286 277
Size: 206 2055 704 1044 2782
Fragments arranged by size:
2782 2055 1044 704 206
XmaI C'CCGG_G
Cuts at: 3667 3811 3667
Size: 144 6647
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmBI BspEI BstZ17I ClaI FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SfiI SnaBI SrfI
SwaI XhoI XmnI ZraI