Restriction map for
pF1KB7295
(Circular) (Six-base) MAPSORT of: pF1KB7295.seq Check: 4731 from: 1 to: 5780
pF1KB7295 5780 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1474 1542 2319 2415 1474
Size: 68 777 96 4839
Fragments arranged by size:
4839 777 96 68
AccI GT'mk_AC
Cuts at: 1055 2818 1055
Size: 1763 4017
Acc65I G'GTAC_C
Cuts at: 2796 2796
Size: 5780
AclI AA'CG_TT
Cuts at: 5620 5620
Size: 5780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 3605 4037 4784 5052 720
Size: 2885 432 747 268 1448
Fragments arranged by size:
2885 1448 747 432 268
AflIII A'CryG_T
Cuts at: 1565 3132 4236 5213 1565
Size: 1567 1104 977 2132
Fragments arranged by size:
2132 1567 1104 977
AgeI A'CCGG_T
Cuts at: 4168 4168
Size: 5780
AhdI GACnn_n'nnGTC
Cuts at: 993 993
Size: 5780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1246 1246
Size: 5780
AlwNI CAG_nnn'CTG
Cuts at: 1266 1512 3085 4652 1266
Size: 246 1573 1567 2394
Fragments arranged by size:
2394 1573 1567 246
ApaI G_GGCC'C
Cuts at: 247 254 449 457 470 509 559 1682 2402
Size: 7 195 8 13 39 50 1123 720
Cuts at: 2402 2429 2525 2657 247
Size: 27 96 132 3370
Fragments arranged by size:
3370 1123 720 195 132 96 50 39
27 13 8 7
ApaLI G'TGCA_C
Cuts at: 4550 4550
Size: 5780
ApoI r'AATT_y
Cuts at: 2106 2784 5133 5389 2106
Size: 678 2349 256 2497
Fragments arranged by size:
2497 2349 678 256
AscI GG'CGCG_CC
Cuts at: 2055 2453 2055
Size: 398 5382
AseI AT'TA_AT
Cuts at: 20 20
Size: 5780
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
AvaI C'yCGr_G
Cuts at: 136 147 240 247 275 369 448 501 943
Size: 11 93 7 28 94 79 53 442
Cuts at: 943 1411 1949 2145 2394 2422 2448 2468 2669
Size: 468 538 196 249 28 26 20 201
Cuts at: 2669 2800 136
Size: 131 3116
Fragments arranged by size:
3116 538 468 442 249 201 196 131
94 93 79 53 28 28 26 20
11 7
BamHI G'GATC_C
Cuts at: 2805 2805
Size: 5780
BanI G'GyrC_C
Cuts at: 364 1269 1407 1459 1526 1831 2472 2596 2716
Size: 905 138 52 67 305 641 124 120
Cuts at: 2716 2796 3210 3458 3493 364
Size: 80 414 248 35 2651
Fragments arranged by size:
2651 905 641 414 305 248 138 124
120 80 67 52 35
BanII G_rGCy'C
Cuts at: 148 247 254 449 457 470 509 559 1673
Size: 99 7 195 8 13 39 50 1114
Cuts at: 1673 1682 2402 2429 2525 2657 2794 3824 148
Size: 9 720 27 96 132 137 1030 2104
Fragments arranged by size:
2104 1114 1030 720 195 137 132 99
96 50 39 27 13 9 8 7
BbeI G_GCGC'C
Cuts at: 1411 1530 2600 2720 3214 3462 1411
Size: 119 1070 120 494 248 3729
Fragments arranged by size:
3729 1070 494 248 120 119
BbsI GAAGACnn'nnnn_
Cuts at: 1791 1791
Size: 5780
BbvCI CC'TCA_GC
Cuts at: 1102 1102
Size: 5780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 894 928 960 994 894
Size: 34 32 34 5680
Fragments arranged by size:
5680 34 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 3678 4439 5780 3678
Size: 761 1341 3678
Fragments arranged by size:
3678 1341 761
BclI T'GATC_A
Cuts at: 3300 3300
Size: 5780
BglI GCCn_nnn'nGGC
Cuts at: 323 362 404 608 2397 2450 323
Size: 39 42 204 1789 53 3653
Fragments arranged by size:
3653 1789 204 53 42 39
BglII A'GATC_T
Cuts at: 1163 1934 3295 5332 1163
Size: 771 1361 2037 1611
Fragments arranged by size:
2037 1611 1361 771
BlpI GC'TnA_GC
Cuts at: 1444 2005 2892 1444
Size: 561 887 4332
Fragments arranged by size:
4332 887 561
Bme1580I G_kGCm'C
Cuts at: 247 254 449 457 470 509 559 1682 2402
Size: 7 195 8 13 39 50 1123 720
Cuts at: 2402 2429 2475 2525 2657 3405 3498 4554 247
Size: 27 46 50 132 748 93 1056 1473
Fragments arranged by size:
1473 1123 1056 748 720 195 132 93
50 50 46 39 27 13 8 7
BmrI ACTGGGnnnn_n'
Cuts at: 756 790 1254 2992 3073 3408 5552 756
Size: 34 464 1738 81 335 2144 984
Fragments arranged by size:
2144 1738 984 464 335 81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4147 4147
Size: 5780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2053
Size: 5780
Bpu10I CC'TnA_GC
Cuts at: 1102 2545 1102
Size: 1443 4337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1771 2956 3835 4327 4625 1771
Size: 1185 879 492 298 2926
Fragments arranged by size:
2926 1185 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 817 861 5484 817
Size: 44 4623 1113
Fragments arranged by size:
4623 1113 44
BsaAI yAC'GTr
Cuts at: 974 3133 974
Size: 2159 3621
BsaBI GATnn'nnATC
Cuts at: 6 1204 3294 5331 6
Size: 1198 2090 2037 455
Fragments arranged by size:
2090 2037 1198 455
BsaHI Gr'CG_yC
Cuts at: 629 1408 1527 2597 2717 3211 3459 4161 5657
Size: 779 119 1070 120 494 248 702 1496
Cuts at: 5657 629
Size: 752
Fragments arranged by size:
1496 1070 779 752 702 494 248 120
119
BsaWI w'CCGG_w
Cuts at: 2969 3490 4168 4442 4589 5000 2969
Size: 521 678 274 147 411 3749
Fragments arranged by size:
3749 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 562 1174 1509 1705 2050 547
Size: 15 612 335 196 345 4277
Fragments arranged by size:
4277 612 345 335 196 15
BseYI C'CCAG_C
Cuts at: 263 1301 2226 2518 2683 3205 3963 4540 5036
Size: 1038 925 292 165 522 758 577 496
Cuts at: 5036 263
Size: 1007
Fragments arranged by size:
1038 1007 925 758 577 522 496 292
165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 2104 2149 2233 453
Size: 1651 45 84 4000
Fragments arranged by size:
4000 1651 84 45
BsiEI CG_ry'CG
Cuts at: 104 357 1401 2082 2202 2341 3322 3368 4576
Size: 253 1044 681 120 139 981 46 1208
Cuts at: 4576 104
Size: 1308
Fragments arranged by size:
1308 1208 1044 981 681 253 139 120
46
BsiHKAI G_wGCw'C
Cuts at: 385 1625 1673 1883 2794 3572 3762 4554 385
Size: 1240 48 210 911 778 190 792 1611
Fragments arranged by size:
1611 1240 911 792 778 210 190 48
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2059
Size: 5780
Bsp1286I G_dGCh'C
Cuts at: 148 247 254 385 449 457 470 509 559
Size: 99 7 131 64 8 13 39 50
Cuts at: 559 1625 1673 1682 1883 2402 2429 2475 2525
Size: 1066 48 9 201 519 27 46 50
Cuts at: 2525 2657 2794 3405 3498 3572 3762 3824 4554
Size: 132 137 611 93 74 190 62 730
Cuts at: 4554 148
Size: 1374
Fragments arranged by size:
1374 1066 730 611 519 201 190 137
132 131 99 93 74 64 62 50
50 48 46 39 27 13 9 8
7
BspEI T'CCGG_A
Cuts at: 5000 5000
Size: 5780
BspHI T'CATG_A
Cuts at: 4956 4956
Size: 5780
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1474 1542 2262 2319 2415 2830 3044 3346 3727
Size: 68 720 57 96 415 214 302 381
Cuts at: 3727 1474
Size: 3527
Fragments arranged by size:
3527 720 415 381 302 214 96 68
57
BsrBI CCG'CTC
Cuts at: 50 2343 4072 4126 5610 5775 50
Size: 2293 1729 54 1484 165 55
Fragments arranged by size:
2293 1729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3692 3692
Size: 5780
BsrFI r'CCGG_y
Cuts at: 326 525 901 1400 2722 3778 3959 4168 326
Size: 199 376 499 1322 1056 181 209 1938
Fragments arranged by size:
1938 1322 1056 499 376 209 199 181
BssHII G'CGCG_C
Cuts at: 115 611 2055 2453 2536 2749 115
Size: 496 1444 398 83 213 3146
Fragments arranged by size:
3146 1444 496 398 213 83
BssSI C'ACGA_G
Cuts at: 348 483 1332 2344 2377 4051 4409 348
Size: 135 849 1012 33 1674 358 1719
Fragments arranged by size:
1719 1674 1012 849 358 135 33
BstAPI GCAn_nnn'nTGC
Cuts at: 2127 3157 2127
Size: 1030 4750
BstBI TT'CG_AA
Cuts at: 4141 4141
Size: 5780
BstEII G'GTnAC_C
Cuts at: 2274 2274
Size: 5780
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 1008
Size: 5780
BstYI r'GATC_y
Cuts at: 1 1097 1163 1205 1604 1934 2805 3272 3295
Size: 1096 66 42 399 330 871 467 23
Cuts at: 3295 3630 3876 4888 4974 4986 5332 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1096 1012 871 467 449 399 346 335
330 246 86 66 42 23 12
BtgI C'CryG_G
Cuts at: 107 319 440 489 671 835 1007 2476 2609
Size: 212 121 49 182 164 172 1469 133
Cuts at: 2609 107
Size: 3278
Fragments arranged by size:
3278 1469 212 182 172 164 133 121
49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2271 3730 3911 4074 2271
Size: 1459 181 163 3977
Fragments arranged by size:
3977 1459 181 163
BtsI GCAGTG_nn'
Cuts at: 436 827 1493 3023 436
Size: 391 666 1530 3193
Fragments arranged by size:
3193 1530 666 391
DraI TTT'AAA
Cuts at: 2779 2779
Size: 5780
DraIII CAC_nnn'GTG
Cuts at: 1869 2127 1869
Size: 258 5522
DrdI GACnn_nn'nnGTC
Cuts at: 1051 3486 4344 1051
Size: 2435 858 2487
Fragments arranged by size:
2487 2435 858
EaeI y'GGCC_r
Cuts at: 354 386 1187 1398 1485 1769 2072 2282 2309
Size: 32 801 211 87 284 303 210 27
Cuts at: 2309 2338 2627 3365 3539 3930 3957 5302 354
Size: 29 289 738 174 391 27 1345 832
Fragments arranged by size:
1345 832 801 738 391 303 289 284
211 210 174 87 32 29 27 27
EagI C'GGCC_G
Cuts at: 354 1398 2338 3365 354
Size: 1044 940 1027 2769
Fragments arranged by size:
2769 1044 1027 940
EarI CTCTTCn'nnn_
Cuts at: 1366 2251 3803 4013 1366
Size: 885 1552 210 3133
Fragments arranged by size:
3133 1552 885 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 544 1585 2559 3988 4298 4444 5590 319
Size: 225 1041 974 1429 310 146 1146 509
Fragments arranged by size:
1429 1146 1041 974 509 310 225 146
EcoICRI GAG'CTC
Cuts at: 1671 2792 1671
Size: 1121 4659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 2053 3605 4037 4784 5052 720
Size: 1333 1552 432 747 268 1448
Fragments arranged by size:
1552 1448 1333 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1687 2122 1687
Size: 435 5345
EcoO109I rG'GnC_Cy
Cuts at: 244 445 453 466 505 707 1679 2398 2426
Size: 201 8 13 39 202 972 719 28
Cuts at: 2426 2653 2919 244
Size: 227 266 3105
Fragments arranged by size:
3105 972 719 266 227 202 201 39
28 13 8
EcoRI G'AATT_C
Cuts at: 2106 2784 2106
Size: 678 5102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1869 3799 1869
Size: 1930 3850
FspI TGC'GCA
Cuts at: 970 1078 3561 970
Size: 108 2483 3189
Fragments arranged by size:
3189 2483 108
HaeII r_GCGC'y
Cuts at: 603 1411 1530 2362 2600 2720 3214 3462 4484
Size: 808 119 832 238 120 494 248 1022
Cuts at: 4484 5463 603
Size: 979 920
Fragments arranged by size:
1022 979 920 832 808 494 248 238
120 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 979 1011 1197 1229 2128 2160 2692 2724 3622
Size: 32 186 32 899 32 532 32 898
Cuts at: 3622 3652 3654 3684 979
Size: 30 2 30 3075
Fragments arranged by size:
3075 899 898 532 186 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 633 1701 2819 633
Size: 1068 1118 3594
Fragments arranged by size:
3594 1118 1068
KasI G'GCGC_C
Cuts at: 1407 1526 2596 2716 3210 3458 1407
Size: 119 1070 120 494 248 3729
Fragments arranged by size:
3729 1070 494 248 120 119
KpnI G_GTAC'C
Cuts at: 2800 2800
Size: 5780
MluI A'CGCG_T
Cuts at: 5213 5213
Size: 5780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2792 4451 4635 2792
Size: 1659 184 3937
Fragments arranged by size:
3937 1659 184
MscI TGG'CCA
Cuts at: 1189 2074 3541 1189
Size: 885 1467 3428
Fragments arranged by size:
3428 1467 885
MslI CAynn'nnrTG
Cuts at: 796 1006 1222 1453 1771 3769 3896 796
Size: 210 216 231 318 1998 127 2680
Fragments arranged by size:
2680 1998 318 231 216 210 127
MspA1I CmG'CkG
Cuts at: 321 442 491 1588 1737 2355 2478 2611 2665
Size: 121 49 1097 149 618 123 133 54
Cuts at: 2665 2891 3205 3565 4578 4823 321
Size: 226 314 360 1013 245 1278
Fragments arranged by size:
1278 1097 1013 618 360 314 245 226
149 133 123 121 54 49
NaeI GCC'GGC
Cuts at: 328 527 1402 2724 3961 328
Size: 199 875 1322 1237 2147
Fragments arranged by size:
2147 1322 1237 875 199
NarI GG'CG_CC
Cuts at: 1408 1527 2597 2717 3211 3459 1408
Size: 119 1070 120 494 248 3729
Fragments arranged by size:
3729 1070 494 248 120 119
NcoI C'CATG_G
Cuts at: 107 107
Size: 5780
NgoMIV G'CCGG_C
Cuts at: 326 525 1400 2722 3959 326
Size: 199 875 1322 1237 2147
Fragments arranged by size:
2147 1322 1237 875 199
NotI GC'GGCC_GC
Cuts at: 354 2338 354
Size: 1984 3796
NspI r_CATG'y
Cuts at: 1383 1569 2833 3333 4240 1383
Size: 186 1264 500 907 2923
Fragments arranged by size:
2923 1264 907 500 186
PciI A'CATG_T
Cuts at: 1565 4236 1565
Size: 2671 3109
PflMI CCAn_nnn'nTGG
Cuts at: 1512 1512
Size: 5780
PfoI T'CCnGG_A
Cuts at: 935 2180 935
Size: 1245 4535
PmeI GTTT'AAAC
Cuts at: 2779 2779
Size: 5780
PmlI CAC'GTG
Cuts at: 974 974
Size: 5780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 4919
Size: 32 5748
PspOMI G'GGCC_C
Cuts at: 243 250 445 453 466 505 555 1678 2398
Size: 7 195 8 13 39 50 1123 720
Cuts at: 2398 2425 2521 2653 243
Size: 27 96 132 3370
Fragments arranged by size:
3370 1123 720 195 132 96 50 39
27 13 8 7
PstI C_TGCA'G
Cuts at: 623 1262 1283 2300 2827 3512 623
Size: 639 21 1017 527 685 2891
Fragments arranged by size:
2891 1017 685 639 527 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5780
PvuII CAG'CTG
Cuts at: 1588 3205 3565 1588
Size: 1617 360 3803
Fragments arranged by size:
3803 1617 360
RsrII CG'GwC_CG
Cuts at: 2691 3975 2691
Size: 1284 4496
SacI G_AGCT'C
Cuts at: 1673 2794 1673
Size: 1121 4659
SacII CC_GC'GG
Cuts at: 322 443 492 2479 2612 322
Size: 121 49 1987 133 3490
Fragments arranged by size:
3490 1987 133 121 49
SalI G'TCGA_C
Cuts at: 2817 2817
Size: 5780
SapI GCTCTTCn'nnn_
Cuts at: 3803 4013 3803
Size: 210 5570
SbfI CC_TGCA'GG
Cuts at: 2827 2827
Size: 5780
ScaI AGT'ACT
Cuts at: 5297 5297
Size: 5780
SexAI A'CCwGG_T
Cuts at: 881 881
Size: 5780
SfcI C'TryA_G
Cuts at: 33 619 1258 1279 2296 2823 3508 4501 4692
Size: 586 639 21 1017 527 685 993 191
Cuts at: 4692 33
Size: 1121
Fragments arranged by size:
1121 1017 993 685 639 586 527 191
21
SfiI GGCCn_nnn'nGGCC
Cuts at: 323 323
Size: 5780
SfoI GGC'GCC
Cuts at: 1409 1528 2598 2718 3212 3460 1409
Size: 119 1070 120 494 248 3729
Fragments arranged by size:
3729 1070 494 248 120 119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
SgrAI Cr'CCGG_yG
Cuts at: 2722 2722
Size: 5780
SmaI CCC'GGG
Cuts at: 138 149 242 249 277 371 1951 2396 2424
Size: 11 93 7 28 94 1580 445 28
Cuts at: 2424 2450 2470 2671 2802 138
Size: 26 20 201 131 3116
Fragments arranged by size:
3116 1580 445 201 131 94 93 28
28 26 20 11 7
SmlI C'TyrA_G
Cuts at: 1786 2935 3850 4342 4604 1786
Size: 1149 915 492 262 2962
Fragments arranged by size:
2962 1149 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5780
SphI G_CATG'C
Cuts at: 2833 3333 2833
Size: 500 5280
SrfI GCCC'GGGC
Cuts at: 149 2671 149
Size: 2522 3258
StyI C'CwwG_G
Cuts at: 107 389 470 2914 3268 107
Size: 282 81 2444 354 2619
Fragments arranged by size:
2619 2444 354 282 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1293 1447 3363 3496 3955 4164 1293
Size: 154 1916 133 459 209 2909
Fragments arranged by size:
2909 1916 459 209 154 133
TatI w'GTAC_w
Cuts at: 1346 1544 1562 1850 5295 1346
Size: 198 18 288 3445 1831
Fragments arranged by size:
3445 1831 288 198 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1121 1153 5297 5329 1121
Size: 32 4144 32 1572
Fragments arranged by size:
4144 1572 32 32
Tth111I GACn'n_nGTC
Cuts at: 3577 3577
Size: 5780
XbaI T'CTAG_A
Cuts at: 2811 2811
Size: 5780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1317 2082 2646 3275 1317
Size: 765 564 629 3822
Fragments arranged by size:
3822 765 629 564
XmaI C'CCGG_G
Cuts at: 136 147 240 247 275 369 1949 2394 2422
Size: 11 93 7 28 94 1580 445 28
Cuts at: 2422 2448 2468 2669 2800 136
Size: 26 20 201 131 3116
Fragments arranged by size:
3116 1580 445 201 131 94 93 28
28 26 20 11 7
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AvrII BaeI BfrBI
BmgBI BmtI BsaXI BsiWI BsmI BsrGI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PpuMI PshAI
PsiI PspXI PsrI SanDI SnaBI SspI StuI SwaI
XhoI XmnI ZraI