Restriction map for
pF1KB7288
(Circular) (Six-base) MAPSORT of: pF1KB7288.seq Check: 2240 from: 1 to: 6746
pF1KB7288 6746 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 479 1912 2366 2904 479
Size: 1433 454 538 4321
Fragments arranged by size:
4321 1433 538 454
AccI GT'mk_AC
Cuts at: 2375 2636 2672 3784 2375
Size: 261 36 1112 5337
Fragments arranged by size:
5337 1112 261 36
Acc65I G'GTAC_C
Cuts at: 3762 3762
Size: 6746
AclI AA'CG_TT
Cuts at: 6586 6586
Size: 6746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2148 2707 4571 5003 5750 6018 2148
Size: 559 1864 432 747 268 2876
Fragments arranged by size:
2876 1864 747 559 432 268
AflIII A'CryG_T
Cuts at: 3458 4098 5202 6179 3458
Size: 640 1104 977 4025
Fragments arranged by size:
4025 1104 977 640
AgeI A'CCGG_T
Cuts at: 5134 5134
Size: 6746
AleI CACnn'nnGTG
Cuts at: 547 1846 3409 547
Size: 1299 1563 3884
Fragments arranged by size:
3884 1563 1299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1259 2072 2932 1259
Size: 813 860 5073
Fragments arranged by size:
5073 860 813
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 655 687 655
Size: 32 6714
AlwNI CAG_nnn'CTG
Cuts at: 629 786 1122 1473 2171 2270 3240 4051 5618
Size: 157 336 351 698 99 970 811 1567
Cuts at: 5618 629
Size: 1757
Fragments arranged by size:
1757 1567 970 811 698 351 336 157
99
ApaI G_GGCC'C
Cuts at: 254 1703 2539 2685 3056 254
Size: 1449 836 146 371 3944
Fragments arranged by size:
3944 1449 836 371 146
ApaLI G'TGCA_C
Cuts at: 1614 1724 2968 5516 1614
Size: 110 1244 2548 2844
Fragments arranged by size:
2844 2548 1244 110
ApoI r'AATT_y
Cuts at: 3750 6099 6355 3750
Size: 2349 256 4141
Fragments arranged by size:
4141 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
AvaI C'yCGr_G
Cuts at: 390 505 562 769 864 1551 1908 2585 2998
Size: 115 57 207 95 687 357 677 413
Cuts at: 2998 3766 390
Size: 768 3370
Fragments arranged by size:
3370 768 687 677 413 357 207 115
95 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 958 991 958
Size: 33 6713
BamHI G'GATC_C
Cuts at: 111 3771 111
Size: 3660 3086
BanI G'GyrC_C
Cuts at: 285 501 538 1030 1413 2548 3088 3762 4176
Size: 216 37 492 383 1135 540 674 414
Cuts at: 4176 4424 4459 285
Size: 248 35 2572
Fragments arranged by size:
2572 1135 674 540 492 414 383 248
216 37 35
BanII G_rGCy'C
Cuts at: 254 366 557 1703 2539 2685 3029 3056 3664
Size: 112 191 1146 836 146 344 27 608
Cuts at: 3664 3701 3760 4790 254
Size: 37 59 1030 2210
Fragments arranged by size:
2210 1146 1030 836 608 344 191 146
112 59 37 27
BbeI G_GCGC'C
Cuts at: 505 1417 4180 4428 505
Size: 912 2763 248 2823
Fragments arranged by size:
2823 2763 912 248
BbsI GAAGACnn'nnnn_
Cuts at: 2240 2240
Size: 6746
BbvCI CC'TCA_GC
Cuts at: 823 1311 823
Size: 488 6258
BciVI GTATCCnnnnn_n'
Cuts at: 4644 5405 6746 4644
Size: 761 1341 4644
Fragments arranged by size:
4644 1341 761
BclI T'GATC_A
Cuts at: 4266 4266
Size: 6746
BglI GCCn_nnn'nGGC
Cuts at: 258 370 258
Size: 112 6634
BglII A'GATC_T
Cuts at: 3275 4261 6298 3275
Size: 986 2037 3723
Fragments arranged by size:
3723 2037 986
BlpI GC'TnA_GC
Cuts at: 622 716 1474 3858 622
Size: 94 758 2384 3510
Fragments arranged by size:
3510 2384 758 94
Bme1580I G_kGCm'C
Cuts at: 254 263 290 541 879 1035 1618 1703 1728
Size: 9 27 251 338 156 583 85 25
Cuts at: 1728 2290 2539 2685 2972 3056 3485 4371 4464
Size: 562 249 146 287 84 429 886 93
Cuts at: 4464 5520 254
Size: 1056 1480
Fragments arranged by size:
1480 1056 886 583 562 429 338 287
251 249 156 146 93 85 84 27
25 9
BmgBI CAC'GTC
Cuts at: 588 758 2448 3459 588
Size: 170 1690 1011 3875
Fragments arranged by size:
3875 1690 1011 170
BmrI ACTGGGnnnn_n'
Cuts at: 2724 3057 3472 3958 4039 4374 6518 2724
Size: 333 415 486 81 335 2144 2952
Fragments arranged by size:
2952 2144 486 415 335 333 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5113 5113
Size: 6746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1144 2205 2431 3531 3547 1135
Size: 9 1061 226 1100 16 4334
Fragments arranged by size:
4334 1100 1061 226 16 9
Bpu10I CC'TnA_GC
Cuts at: 823 1069 1311 823
Size: 246 242 6258
Fragments arranged by size:
6258 246 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4801 5293 5591 3922
Size: 879 492 298 5077
Fragments arranged by size:
5077 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 455 2089 2595 2702 6450 455
Size: 1634 506 107 3748 751
Fragments arranged by size:
3748 1634 751 506 107
BsaAI yAC'GTr
Cuts at: 327 966 1344 1831 3228 4099 327
Size: 639 378 487 1397 871 2974
Fragments arranged by size:
2974 1397 871 639 487 378
BsaBI GATnn'nnATC
Cuts at: 6 4260 6297 6
Size: 4254 2037 455
Fragments arranged by size:
4254 2037 455
BsaHI Gr'CG_yC
Cuts at: 302 502 839 1414 2021 2159 2378 2990 4177
Size: 200 337 575 607 138 219 612 1187
Cuts at: 4177 4425 5127 6623 302
Size: 248 702 1496 425
Fragments arranged by size:
1496 1187 702 612 607 575 425 337
248 219 200 138
BsaWI w'CCGG_w
Cuts at: 383 653 1269 1625 3269 3935 4456 5134 5408
Size: 270 616 356 1644 666 521 678 274
Cuts at: 5408 5555 5966 383
Size: 147 411 1163
Fragments arranged by size:
1644 1163 678 666 616 521 411 356
274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 655 685 747 777 1291 1321 1474 1504 2095
Size: 30 62 30 514 30 153 30 591
Cuts at: 2095 2125 2479 2509 655
Size: 30 354 30 4892
Fragments arranged by size:
4892 591 514 354 153 62 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 427 430 600 676 679 682 755 862 1299
Size: 3 170 76 3 3 73 107 437
Cuts at: 1299 1993 3163 427
Size: 694 1170 4010
Fragments arranged by size:
4010 1170 694 437 170 107 76 73
3 3 3
BseYI C'CCAG_C
Cuts at: 921 1139 1347 1398 1544 1796 2024 2849 4171
Size: 218 208 51 146 252 228 825 1322
Cuts at: 4171 4929 5506 6002 921
Size: 758 577 496 1665
Fragments arranged by size:
1665 1322 825 758 577 496 252 228
218 208 146 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 964 1266 1444 1471 1543 1677 451
Size: 513 302 178 27 72 134 5520
Fragments arranged by size:
5520 513 302 178 134 72 27
BsiEI CG_ry'CG
Cuts at: 104 2514 3658 4288 4334 5542 104
Size: 2410 1144 630 46 1208 1308
Fragments arranged by size:
2410 1308 1208 1144 630 46
BsiHKAI G_wGCw'C
Cuts at: 854 965 1361 1618 1728 2015 2051 2707 2972
Size: 111 396 257 110 287 36 656 265
Cuts at: 2972 3029 3070 3110 3701 3760 4538 4728 5520
Size: 57 41 40 591 59 778 190 792
Cuts at: 5520 854
Size: 2080
Fragments arranged by size:
2080 792 778 656 591 396 287 265
257 190 111 110 59 57 41 40
36
BsmI GAATG_Cn'
Cuts at: 2848 2933 2848
Size: 85 6661
BsmBI CGTCTCn'nnnn_
Cuts at: 578 2495 2512 578
Size: 1917 17 4812
Fragments arranged by size:
4812 1917 17
Bsp1286I G_dGCh'C
Cuts at: 254 263 290 366 541 557 854 879 965
Size: 9 27 76 175 16 297 25 86
Cuts at: 965 1035 1361 1618 1703 1728 2015 2051 2290
Size: 70 326 257 85 25 287 36 239
Cuts at: 2290 2539 2685 2707 2972 3029 3056 3070 3110
Size: 249 146 22 265 57 27 14 40
Cuts at: 3110 3485 3664 3701 3760 4371 4464 4538 4728
Size: 375 179 37 59 611 93 74 190
Cuts at: 4728 4790 5520 254
Size: 62 730 1480
Fragments arranged by size:
1480 730 611 375 326 297 287 265
257 249 239 190 179 175 146 93
86 85 76 74 70 62 59 57
40 37 36 27 27 25 25 22
16 14 9
BspEI T'CCGG_A
Cuts at: 383 653 1625 3269 5966 383
Size: 270 972 1644 2697 1163
Fragments arranged by size:
2697 1644 1163 972 270
BspHI T'CATG_A
Cuts at: 989 5922 989
Size: 4933 1813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 473 479 986 1493 1912 2366 2904 3181 3796
Size: 6 507 507 419 454 538 277 615
Cuts at: 3796 4010 4312 4693 473
Size: 214 302 381 2526
Fragments arranged by size:
2526 615 538 507 507 454 419 381
302 277 214 6
BsrBI CCG'CTC
Cuts at: 50 130 186 268 2963 3099 5038 5092 6576
Size: 80 56 82 2695 136 1939 54 1484
Cuts at: 6576 6741 50
Size: 165 55
Fragments arranged by size:
2695 1939 1484 165 136 82 80 56
55 54
BsrDI GCAATG_nn'
Cuts at: 1585 2471 2991 4658 1585
Size: 886 520 1667 3673
Fragments arranged by size:
3673 1667 886 520
BsrFI r'CCGG_y
Cuts at: 117 592 746 1255 1533 4744 4925 5134 117
Size: 475 154 509 278 3211 181 209 1729
Fragments arranged by size:
3211 1729 509 475 278 209 181 154
BsrGI T'GTAC_A
Cuts at: 3392 3392
Size: 6746
BssSI C'ACGA_G
Cuts at: 496 2044 5017 5375 496
Size: 1548 2973 358 1867
Fragments arranged by size:
2973 1867 1548 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2828 4123 2828
Size: 1295 5451
BstBI TT'CG_AA
Cuts at: 5107 5107
Size: 6746
BstEII G'GTnAC_C
Cuts at: 2300 2300
Size: 6746
BstXI CCAn_nnnn'nTGG
Cuts at: 549 1336 2484 2896 549
Size: 787 1148 412 4399
Fragments arranged by size:
4399 1148 787 412
BstYI r'GATC_y
Cuts at: 1 111 2336 3207 3275 3771 4238 4261 4596
Size: 110 2225 871 68 496 467 23 335
Cuts at: 4596 4842 5854 5940 5952 6298 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2225 1012 871 496 467 449 346 335
246 110 86 68 23 12
BtgI C'CryG_G
Cuts at: 107 951 2477 2531 2544 2781 3119 3494 3609
Size: 844 1526 54 13 237 338 375 115
Cuts at: 3609 107
Size: 3244
Fragments arranged by size:
3244 1526 844 375 338 237 115 54
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 647 4696 4877 5040 647
Size: 4049 181 163 2353
Fragments arranged by size:
4049 2353 181 163
BtsI GCAGTG_nn'
Cuts at: 1752 1990 2268 2979 3291 3989 1752
Size: 238 278 711 312 698 4509
Fragments arranged by size:
4509 711 698 312 278 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 2697 2732 1362
Size: 35 1300 35 5376
Fragments arranged by size:
5376 1300 35 35
DraI TTT'AAA
Cuts at: 1746 3745 1746
Size: 1999 4747
DraIII CAC_nnn'GTG
Cuts at: 330 330
Size: 6746
DrdI GACnn_nn'nnGTC
Cuts at: 2587 2647 2668 4452 5310 2587
Size: 60 21 1784 858 4023
Fragments arranged by size:
4023 1784 858 60 21
EaeI y'GGCC_r
Cuts at: 456 590 748 1338 1531 1886 2396 2511 3100
Size: 134 158 590 193 355 510 115 589
Cuts at: 3100 3505 4331 4505 4896 4923 6268 456
Size: 405 826 174 391 27 1345 934
Fragments arranged by size:
1345 934 826 590 589 510 405 391
355 193 174 158 134 115 27
EagI C'GGCC_G
Cuts at: 2511 4331 2511
Size: 1820 4926
EarI CTCTTCn'nnn_
Cuts at: 439 2432 2651 3214 4769 4979 439
Size: 1993 219 563 1555 210 2206
Fragments arranged by size:
2206 1993 1555 563 219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 104 885 2735 2916 4954 5264 5410 6556 104
Size: 781 1850 181 2038 310 146 1146 294
Fragments arranged by size:
2038 1850 1146 781 310 294 181 146
EcoICRI GAG'CTC
Cuts at: 3027 3699 3758 3027
Size: 672 59 6015
Fragments arranged by size:
6015 672 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1144 2148 2205 2431 2707 3531 3547 4571
Size: 9 1004 57 226 276 824 16 1024
Cuts at: 4571 5003 5750 6018 1135
Size: 432 747 268 1863
Fragments arranged by size:
1863 1024 1004 824 747 432 276 268
226 57 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1423 2656 1423
Size: 1233 5513
EcoO109I rG'GnC_Cy
Cuts at: 251 783 797 915 1699 1700 1859 1998 2536
Size: 532 14 118 784 1 159 139 538
Cuts at: 2536 2682 2726 3053 3170 3531 3885 251
Size: 146 44 327 117 361 354 3112
Fragments arranged by size:
3112 784 538 532 361 354 327 159
146 139 118 117 44 14 1
EcoRI G'AATT_C
Cuts at: 3750 3750
Size: 6746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2178 2445 4765 2178
Size: 267 2320 4159
Fragments arranged by size:
4159 2320 267
FseI GG_CCGG'CC
Cuts at: 596 596
Size: 6746
FspI TGC'GCA
Cuts at: 295 4527 295
Size: 4232 2514
FspAI rTGC'GCAy
Cuts at: 295 295
Size: 6746
HaeII r_GCGC'y
Cuts at: 505 622 1417 1540 1805 4180 4428 5450 6429
Size: 117 795 123 265 2375 248 1022 979
Cuts at: 6429 505
Size: 822
Fragments arranged by size:
2375 1022 979 822 795 265 248 123
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1291 1323 1742 1774 2095 2127 2345 2377 3244
Size: 32 419 32 321 32 218 32 867
Cuts at: 3244 3276 4588 4618 4620 4650 1291
Size: 32 1312 30 2 30 3387
Fragments arranged by size:
3387 1312 867 419 321 218 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2637 3785 2637
Size: 1148 5598
KasI G'GCGC_C
Cuts at: 501 1413 4176 4424 501
Size: 912 2763 248 2823
Fragments arranged by size:
2823 2763 912 248
KpnI G_GTAC'C
Cuts at: 3766 3766
Size: 6746
MluI A'CGCG_T
Cuts at: 6179 6179
Size: 6746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 561 5417 5601 561
Size: 4856 184 1706
Fragments arranged by size:
4856 1706 184
MscI TGG'CCA
Cuts at: 1340 4507 1340
Size: 3167 3579
MslI CAynn'nnrTG
Cuts at: 547 1042 1258 1493 1846 2054 2326 2476 2482
Size: 495 216 235 353 208 272 150 6
Cuts at: 2482 2572 2841 2914 2984 3409 3457 3499 4735
Size: 90 269 73 70 425 48 42 1236
Cuts at: 4735 4862 547
Size: 127 2431
Fragments arranged by size:
2431 1236 495 425 353 272 269 235
216 208 150 127 90 73 70 48
42 6
MspA1I CmG'CkG
Cuts at: 1139 1280 1379 1457 1650 1796 3121 3857 4171
Size: 141 99 78 193 146 1325 736 314
Cuts at: 4171 4531 5544 5789 1139
Size: 360 1013 245 2096
Fragments arranged by size:
2096 1325 1013 736 360 314 245 193
146 141 99 78
NaeI GCC'GGC
Cuts at: 119 594 748 1535 4927 119
Size: 475 154 787 3392 1938
Fragments arranged by size:
3392 1938 787 475 154
NarI GG'CG_CC
Cuts at: 502 1414 4177 4425 502
Size: 912 2763 248 2823
Fragments arranged by size:
2823 2763 912 248
NcoI C'CATG_G
Cuts at: 107 2477 2531 3494 3609 107
Size: 2370 54 963 115 3244
Fragments arranged by size:
3244 2370 963 115 54
NgoMIV G'CCGG_C
Cuts at: 117 592 746 1533 4925 117
Size: 475 154 787 3392 1938
Fragments arranged by size:
3392 1938 787 475 154
NspI r_CATG'y
Cuts at: 736 889 1236 1263 2913 3799 4299 5206 736
Size: 153 347 27 1650 886 500 907 2276
Fragments arranged by size:
2276 1650 907 886 500 347 153 27
PasI CC'CwG_GG
Cuts at: 255 306 834 255
Size: 51 528 6167
Fragments arranged by size:
6167 528 51
PciI A'CATG_T
Cuts at: 5202 5202
Size: 6746
PflMI CCAn_nnn'nTGG
Cuts at: 1347 2394 2772 3436 3615 1347
Size: 1047 378 664 179 4478
Fragments arranged by size:
4478 1047 664 378 179
PfoI T'CCnGG_A
Cuts at: 2651 2651
Size: 6746
PmeI GTTT'AAAC
Cuts at: 3745 3745
Size: 6746
PmlI CAC'GTG
Cuts at: 327 1344 327
Size: 1017 5729
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 655 687 5885 5917 655
Size: 32 5198 32 1484
Fragments arranged by size:
5198 1484 32 32
PpuMI rG'GwC_Cy
Cuts at: 915 1859 1998 2726 3170 3531 915
Size: 944 139 728 444 361 4130
Fragments arranged by size:
4130 944 728 444 361 139
PspOMI G'GGCC_C
Cuts at: 250 1699 2535 2681 3052 250
Size: 1449 836 146 371 3944
Fragments arranged by size:
3944 1449 836 371 146
PspXI vC'TCGA_Gb
Cuts at: 864 864
Size: 6746
PstI C_TGCA'G
Cuts at: 1462 1939 2191 2346 2528 3019 3178 3793 4478
Size: 477 252 155 182 491 159 615 685
Cuts at: 4478 1462
Size: 3730
Fragments arranged by size:
3730 685 615 491 477 252 182 159
155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6746
PvuII CAG'CTG
Cuts at: 1280 1650 1796 4171 4531 1280
Size: 370 146 2375 360 3495
Fragments arranged by size:
3495 2375 370 360 146
RsrII CG'GwC_CG
Cuts at: 381 1272 4941 381
Size: 891 3669 2186
Fragments arranged by size:
3669 2186 891
SacI G_AGCT'C
Cuts at: 3029 3701 3760 3029
Size: 672 59 6015
Fragments arranged by size:
6015 672 59
SacII CC_GC'GG
Cuts at: 3122 3122
Size: 6746
SalI G'TCGA_C
Cuts at: 2635 3783 2635
Size: 1148 5598
SanDI GG'GwC_CC
Cuts at: 2726 2726
Size: 6746
SapI GCTCTTCn'nnn_
Cuts at: 3214 4769 4979 3214
Size: 1555 210 4981
Fragments arranged by size:
4981 1555 210
SbfI CC_TGCA'GG
Cuts at: 2346 3178 3793 2346
Size: 832 615 5299
Fragments arranged by size:
5299 832 615
ScaI AGT'ACT
Cuts at: 6263 6263
Size: 6746
SfcI C'TryA_G
Cuts at: 33 1458 1935 2187 2342 2524 3015 3174 3318
Size: 1425 477 252 155 182 491 159 144
Cuts at: 3318 3488 3789 4474 5467 5658 33
Size: 170 301 685 993 191 1121
Fragments arranged by size:
1425 1121 993 685 491 477 301 252
191 182 170 159 155 144
SfoI GGC'GCC
Cuts at: 503 1415 4178 4426 503
Size: 912 2763 248 2823
Fragments arranged by size:
2823 2763 912 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
SgrAI Cr'CCGG_yG
Cuts at: 117 117
Size: 6746
SmaI CCC'GGG
Cuts at: 507 1553 2587 3768 507
Size: 1046 1034 1181 3485
Fragments arranged by size:
3485 1181 1046 1034
SmlI C'TyrA_G
Cuts at: 864 3901 4816 5308 5570 864
Size: 3037 915 492 262 2040
Fragments arranged by size:
3037 2040 915 492 262
SnaBI TAC'GTA
Cuts at: 3228 3228
Size: 6746
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6746
SphI G_CATG'C
Cuts at: 889 1236 2913 3799 4299 889
Size: 347 1677 886 500 3336
Fragments arranged by size:
3336 1677 886 500 347
SspI AAT'ATT
Cuts at: 3314 3314
Size: 6746
StyI C'CwwG_G
Cuts at: 107 686 1025 1706 2060 2477 2531 3297 3494
Size: 579 339 681 354 417 54 766 197
Cuts at: 3494 3609 3880 4234 107
Size: 115 271 354 2619
Fragments arranged by size:
2619 766 681 579 417 354 354 339
271 197 115 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 1018 2319 3106 3587 3651 3672 4329 4462
Size: 250 1301 787 481 64 21 657 133
Cuts at: 4462 4921 5130 768
Size: 459 209 2384
Fragments arranged by size:
2384 1301 787 657 481 459 250 209
133 64 21
TatI w'GTAC_w
Cuts at: 2003 2078 2495 3392 6261 2003
Size: 75 417 897 2869 2488
Fragments arranged by size:
2869 2488 897 417 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 747 779 1707 1739 1760 1792 2224 2256 6263
Size: 32 928 32 21 32 432 32 4007
Cuts at: 6263 6295 747
Size: 32 1198
Fragments arranged by size:
4007 1198 928 432 32 32 32 32
32 21
Tth111I GACn'n_nGTC
Cuts at: 4543 4543
Size: 6746
XbaI T'CTAG_A
Cuts at: 3777 3777
Size: 6746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2121 2391 2559 3492 4241 2121
Size: 270 168 933 749 4626
Fragments arranged by size:
4626 933 749 270 168
XhoI C'TCGA_G
Cuts at: 864 864
Size: 6746
XmaI C'CCGG_G
Cuts at: 505 1551 2585 3766 505
Size: 1046 1034 1181 3485
Fragments arranged by size:
3485 1181 1046 1034
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BcgI BfrBI
BmtI BsiWI BssHII BstZ17I Bsu36I ClaI EcoRV HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PsrI SexAI SfiI SrfI StuI SwaI
XmnI ZraI