Restriction map for
pF1KB7276
(Circular) (Six-base) MAPSORT of: pF1KB7276.seq Check: 9440 from: 1 to: 4259
pF1KB7276 4259 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 801 1054 2084 2516 3263 3531 541
Size: 260 253 1030 432 747 268 1269
Fragments arranged by size:
1269 1030 747 432 268 260 253
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 459 1564 3131 459
Size: 1105 1567 1587
Fragments arranged by size:
1587 1567 1105
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 561 841 963 1246 1263 3612 3868 561
Size: 280 122 283 17 2349 256 952
Fragments arranged by size:
2349 952 283 280 256 122 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 521 1279 521
Size: 758 3501
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1275 1689 1937 1972 1275
Size: 414 248 35 3562
Fragments arranged by size:
3562 414 248 35
BanII G_rGCy'C
Cuts at: 1273 2303 1273
Size: 1030 3229
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BfrBI ATG'CAT
Cuts at: 295 295
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1371 1371
Size: 4259
Bme1580I G_kGCm'C
Cuts at: 1884 1977 3033 1884
Size: 93 1056 3110
Fragments arranged by size:
3110 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1471 1552 1887 4031 1471
Size: 81 335 2144 1699
Fragments arranged by size:
2144 1699 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2314 2806 3104 1435
Size: 879 492 298 2590
Fragments arranged by size:
2590 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3963 3963
Size: 4259
BsaAI yAC'GTr
Cuts at: 996 1612 996
Size: 616 3643
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 889 889
Size: 4259
BseYI C'CCAG_C
Cuts at: 1684 2442 3019 3515 1684
Size: 758 577 496 2428
Fragments arranged by size:
2428 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1163
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1273 2051 2241 3033 1273
Size: 778 190 792 2499
Fragments arranged by size:
2499 792 778 190
BsmI GAATG_Cn'
Cuts at: 582 582
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 1273 1884 1977 2051 2241 2303 3033 1273
Size: 611 93 74 190 62 730 2499
Fragments arranged by size:
2499 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1309 1523 1825 2206 1309
Size: 214 302 381 3362
Fragments arranged by size:
3362 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2551 2605 4089 4254 50
Size: 2501 54 1484 165 55
Fragments arranged by size:
2501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 753 1067 2171 753
Size: 314 1104 2841
Fragments arranged by size:
2841 1104 314
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 982 1636 982
Size: 654 3605
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1284 1751 1774 2109 2355 3367 3453 3465
Size: 1283 467 23 335 246 1012 86 12
Cuts at: 3465 3811 1
Size: 346 449
Fragments arranged by size:
1283 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 1146 1502 1146
Size: 356 3903
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 434 1844 2018 2409 2436 3781 434
Size: 1410 174 391 27 1345 912
Fragments arranged by size:
1410 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 866 2282 2492 866
Size: 1416 210 2633
Fragments arranged by size:
2633 1416 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 2777 2923 4069 2467
Size: 310 146 1146 2657
Fragments arranged by size:
2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 801 1054 2084 2516 3263 3531 541
Size: 260 253 1030 432 747 268 1269
Fragments arranged by size:
1269 1030 747 432 268 260 253
EcoNI CCTnn'n_nnAGG
Cuts at: 795 795
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 1398 1398
Size: 4259
EcoRI G'AATT_C
Cuts at: 1246 1263 1246
Size: 17 4242
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 571 645 2278 571
Size: 74 1633 2552
Fragments arranged by size:
2552 1633 74
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 1693 1941 2963 3942 1693
Size: 248 1022 979 2010
Fragments arranged by size:
2010 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 2101 2131 2133 2163 502
Size: 32 1567 30 2 30 2598
Fragments arranged by size:
2598 1567 32 30 30 2
HincII GTy'rAC
Cuts at: 1195 1298 1195
Size: 103 4156
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 230 2930 3114 230
Size: 2700 184 1375
Fragments arranged by size:
2700 1375 184
MscI TGG'CCA
Cuts at: 436 2020 436
Size: 1584 2675
MslI CAynn'nnrTG
Cuts at: 2248 2375 2248
Size: 127 4132
MspA1I CmG'CkG
Cuts at: 1370 1684 2044 3057 3302 1370
Size: 314 360 1013 245 2327
Fragments arranged by size:
2327 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NsiI A_TGCA'T
Cuts at: 297 297
Size: 4259
NspI r_CATG'y
Cuts at: 178 1312 1812 2719 178
Size: 1134 500 907 1718
Fragments arranged by size:
1718 1134 907 500
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PmlI CAC'GTG
Cuts at: 996 996
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PstI C_TGCA'G
Cuts at: 1306 1991 1306
Size: 685 3574
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 1684 2044 1684
Size: 360 3899
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SfcI C'TryA_G
Cuts at: 33 1302 1987 2980 3171 33
Size: 1269 685 993 191 1121
Fragments arranged by size:
1269 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 521 1414 2329 2821 3083 521
Size: 893 915 492 262 1697
Fragments arranged by size:
1697 915 893 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 178 1312 1812 178
Size: 1134 500 2625
Fragments arranged by size:
2625 1134 500
SspI AAT'ATT
Cuts at: 225 468 225
Size: 243 4016
StyI C'CwwG_G
Cuts at: 485 736 1393 1747 485
Size: 251 657 354 2997
Fragments arranged by size:
2997 657 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1842 1975 2434 2643 1842
Size: 133 459 209 3458
Fragments arranged by size:
3458 459 209 133
TatI w'GTAC_w
Cuts at: 1217 3774 1217
Size: 2557 1702
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 2056 2056
Size: 4259
XbaI T'CTAG_A
Cuts at: 1131 1290 1131
Size: 159 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 947 1754 947
Size: 807 3452
XhoI C'TCGA_G
Cuts at: 521 521
Size: 4259
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XmnI
ZraI