Restriction map for
pF1KB7273
(Circular) (Six-base) MAPSORT of: pF1KB7273.seq Check: 3096 from: 1 to: 7109
pF1KB7273 7109 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 1296 1296
Size: 7109
AccI GT'mk_AC
Cuts at: 1296 2750 4147 1296
Size: 1454 1397 4258
Fragments arranged by size:
4258 1454 1397
Acc65I G'GTAC_C
Cuts at: 3915 4125 3915
Size: 210 6899
AclI AA'CG_TT
Cuts at: 6949 6949
Size: 7109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1715 1838 2935 3383 3793 4934 5366 5719
Size: 1592 123 1097 448 410 1141 432 353
Cuts at: 5719 6381 123
Size: 662 851
Fragments arranged by size:
1592 1141 1097 851 662 448 432 410
353 123
AflII C'TTAA_G
Cuts at: 2857 2857
Size: 7109
AflIII A'CryG_T
Cuts at: 629 3868 4461 6261 6542 629
Size: 3239 593 1800 281 1196
Fragments arranged by size:
3239 1800 1196 593 281
AhdI GACnn_n'nnGTC
Cuts at: 175 175
Size: 7109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1652 1652
Size: 7109
AlwNI CAG_nnn'CTG
Cuts at: 3391 3440 4414 5852 3391
Size: 49 974 1438 4648
Fragments arranged by size:
4648 1438 974 49
ApaLI G'TGCA_C
Cuts at: 5947 5947
Size: 7109
ApoI r'AATT_y
Cuts at: 1268 1373 1429 1579 1601 2048 2550 2559 2631
Size: 105 56 150 22 447 502 9 72
Cuts at: 2631 2672 2736 2798 3011 3588 3876 3996 4113
Size: 41 64 62 213 577 288 120 117
Cuts at: 4113 6462 6718 1268
Size: 2349 256 1659
Fragments arranged by size:
2349 1659 577 502 447 288 256 213
150 120 117 105 72 64 62 56
41 22 9
AseI AT'TA_AT
Cuts at: 20 1927 20
Size: 1907 5202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7109
AvaI C'yCGr_G
Cuts at: 4129 4129
Size: 7109
AvrII C'CTAG_G
Cuts at: 3184 3184
Size: 7109
BamHI G'GATC_C
Cuts at: 4134 4134
Size: 7109
BanI G'GyrC_C
Cuts at: 117 1134 1462 3915 4125 4539 4787 4822 117
Size: 1017 328 2453 210 414 248 35 2404
Fragments arranged by size:
2453 2404 1017 414 328 248 210 35
BanII G_rGCy'C
Cuts at: 3536 4123 5153 3536
Size: 587 1030 5492
Fragments arranged by size:
5492 1030 587
BbeI G_GCGC'C
Cuts at: 121 4543 4791 121
Size: 4422 248 2439
Fragments arranged by size:
4422 2439 248
BbsI GAAGACnn'nnnn_
Cuts at: 340 899 1211 1874 2923 340
Size: 559 312 663 1049 4526
Fragments arranged by size:
4526 1049 663 559 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7075
BciVI GTATCCnnnnn_n'
Cuts at: 495 1662 5007 6063 7109 495
Size: 1167 3345 1056 1046 495
Fragments arranged by size:
3345 1167 1056 1046 495
BclI T'GATC_A
Cuts at: 636 764 1505 3573 4629 636
Size: 128 741 2068 1056 3116
Fragments arranged by size:
3116 2068 1056 741 128
BfrBI ATG'CAT
Cuts at: 287 1639 2615 287
Size: 1352 976 4781
Fragments arranged by size:
4781 1352 976
BglI GCCn_nnn'nGGC
Cuts at: 3242 3242
Size: 7109
BglII A'GATC_T
Cuts at: 4624 6661 4624
Size: 2037 5072
BlpI GC'TnA_GC
Cuts at: 2023 4221 2023
Size: 2198 4911
Bme1580I G_kGCm'C
Cuts at: 4734 4827 5951 4734
Size: 93 1124 5892
Fragments arranged by size:
5892 1124 93
BmgBI CAC'GTC
Cuts at: 1123 2458 1123
Size: 1335 5774
BmrI ACTGGGnnnn_n'
Cuts at: 3832 4321 4402 4737 6881 3832
Size: 489 81 335 2144 4060
Fragments arranged by size:
4060 2144 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 534 3662 5476 534
Size: 3128 1814 2167
Fragments arranged by size:
3128 2167 1814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 3026 3449 3761 3953 1322
Size: 1704 423 312 192 4478
Fragments arranged by size:
4478 1704 423 312 192
Bpu10I CC'TnA_GC
Cuts at: 933 2330 933
Size: 1397 5712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4285 5164 5878 6176 4285
Size: 879 714 298 5218
Fragments arranged by size:
5218 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 6813 183
Size: 6630 479
BsaAI yAC'GTr
Cuts at: 3869 4462 3869
Size: 593 6516
BsaBI GATnn'nnATC
Cuts at: 6 3425 3617 4623 6660 6
Size: 3419 192 1006 2037 455
Fragments arranged by size:
3419 2037 1006 455 192
BsaHI Gr'CG_yC
Cuts at: 118 1293 4540 4788 5490 6986 118
Size: 1175 3247 248 702 1496 241
Fragments arranged by size:
3247 1496 1175 702 248 241
BsaWI w'CCGG_w
Cuts at: 4298 4819 5497 5908 6055 6329 4298
Size: 521 678 411 147 274 5078
Fragments arranged by size:
5078 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1295 1325 1901 1931 3891 3921 1295
Size: 30 576 30 1960 30 4483
Fragments arranged by size:
4483 1960 576 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 334 2997 3078 3536 3539 3548 3638 3641
Size: 174 2663 81 458 3 9 90 3
Cuts at: 3641 3881 160
Size: 240 3388
Fragments arranged by size:
3388 2663 458 240 174 90 81 9
3 3
BseYI C'CCAG_C
Cuts at: 293 4534 5292 5957 6365 293
Size: 4241 758 665 408 1037
Fragments arranged by size:
4241 1037 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 2135 2750 1577
Size: 558 615 5936
Fragments arranged by size:
5936 615 558
BsiEI CG_ry'CG
Cuts at: 104 4651 4697 5927 104
Size: 4547 46 1230 1286
Fragments arranged by size:
4547 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1631 3938 4123 4901 5091 5951 1631
Size: 2307 185 778 190 860 2789
Fragments arranged by size:
2789 2307 860 778 190 185
Bsp1286I G_dGCh'C
Cuts at: 1631 3536 3938 4123 4734 4827 4901 5091 5153
Size: 1905 402 185 611 93 74 190 62
Cuts at: 5153 5951 1631
Size: 798 2789
Fragments arranged by size:
2789 1905 798 611 402 190 185 93
74 62
BspHI T'CATG_A
Cuts at: 1023 1241 1586 2429 3576 5541 1023
Size: 218 345 843 1147 1965 2591
Fragments arranged by size:
2591 1965 1147 843 345 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1062 4159 4373 4675 5056 1062
Size: 3097 214 302 381 3115
Fragments arranged by size:
3115 3097 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5401 5455 6939 7104 50
Size: 5351 54 1484 165 55
Fragments arranged by size:
5351 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 1079 5021 365
Size: 714 3942 2453
Fragments arranged by size:
3942 2453 714
BsrFI r'CCGG_y
Cuts at: 5107 5288 5107
Size: 181 6928
BsrGI T'GTAC_A
Cuts at: 4064 4064
Size: 7109
BssSI C'ACGA_G
Cuts at: 5380 6088 5380
Size: 708 6401
BstAPI GCAn_nnn'nTGC
Cuts at: 382 4486 382
Size: 4104 3005
BstBI TT'CG_AA
Cuts at: 5470 5470
Size: 7109
BstEII G'GTnAC_C
Cuts at: 3268 3268
Size: 7109
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 2387
Size: 7109
BstYI r'GATC_y
Cuts at: 1 1305 1959 2165 3426 3735 4134 4601 4624
Size: 1304 654 206 1261 309 399 467 23
Cuts at: 4624 4959 5205 5511 5523 5609 6661 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1304 1261 1052 654 467 449 399 335
309 306 246 206 86 23 12
BstZ17I GTA'TAC
Cuts at: 2751 2751
Size: 7109
Bsu36I CC'TnA_GG
Cuts at: 3252 3252
Size: 7109
BtgI C'CryG_G
Cuts at: 3089 3089
Size: 7109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5059 5240 5403 5059
Size: 181 163 6765
Fragments arranged by size:
6765 181 163
BtsI GCAGTG_nn'
Cuts at: 2776 4352 2776
Size: 1576 5533
ClaI AT'CG_AT
Cuts at: 1388 1388
Size: 7109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2000 2035 2000
Size: 35 7074
DraI TTT'AAA
Cuts at: 533 1095 2838 2945 4108 533
Size: 562 1743 107 1163 3534
Fragments arranged by size:
3534 1743 1163 562 107
DraIII CAC_nnn'GTG
Cuts at: 2878 2878
Size: 7109
DrdI GACnn_nn'nnGTC
Cuts at: 4815 6159 4815
Size: 1344 5765
EaeI y'GGCC_r
Cuts at: 1046 1527 4694 4868 5259 5286 6631 1046
Size: 481 3167 174 391 27 1345 1524
Fragments arranged by size:
3167 1524 1345 481 391 174 27
EagI C'GGCC_G
Cuts at: 4694 4694
Size: 7109
EarI CTCTTCn'nnn_
Cuts at: 227 248 959 1736 3524 3557 3746 5132 5342
Size: 21 711 777 1788 33 189 1386 210
Cuts at: 5342 227
Size: 1994
Fragments arranged by size:
1994 1788 1386 777 711 210 189 33
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3710 5317 6059 6205 6919 3710
Size: 1607 742 146 714 3900
Fragments arranged by size:
3900 1607 742 714 146
EcoICRI GAG'CTC
Cuts at: 4121 4121
Size: 7109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 1322 1715 1838 2935 3026 3383 3449 3761
Size: 1199 393 123 1097 91 357 66 312
Cuts at: 3761 3793 3953 4934 5366 5719 6381 123
Size: 32 160 981 432 353 662 851
Fragments arranged by size:
1199 1097 981 851 662 432 393 357
353 312 160 123 91 66 32
EcoNI CCTnn'n_nnAGG
Cuts at: 190 3230 3909 190
Size: 3040 679 3390
Fragments arranged by size:
3390 3040 679
EcoO109I rG'GnC_Cy
Cuts at: 4248 4248
Size: 7109
EcoRI G'AATT_C
Cuts at: 1429 3996 4113 1429
Size: 2567 117 4425
Fragments arranged by size:
4425 2567 117
EcoRV GAT'ATC
Cuts at: 825 825
Size: 7109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 808 3510 5128 808
Size: 2702 1618 2789
Fragments arranged by size:
2789 2702 1618
FspI TGC'GCA
Cuts at: 4890 4890
Size: 7109
HaeII r_GCGC'y
Cuts at: 121 4543 4791 6021 6792 121
Size: 4422 248 1230 771 438
Fragments arranged by size:
4422 1230 771 438 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 327 359 364 396 2424 2456 3322
Size: 32 134 32 5 32 2028 32 866
Cuts at: 3322 3354 3531 3563 4951 4981 4983 5013 161
Size: 32 177 32 1388 30 2 30 2257
Fragments arranged by size:
2257 2028 1388 866 177 134 32 32
32 32 32 32 30 30 5 2
HincII GTy'rAC
Cuts at: 1297 1940 4148 1297
Size: 643 2208 4258
Fragments arranged by size:
4258 2208 643
HindIII A'AGCT_T
Cuts at: 3306 3306
Size: 7109
HpaI GTT'AAC
Cuts at: 1940 1940
Size: 7109
KasI G'GCGC_C
Cuts at: 117 4539 4787 117
Size: 4422 248 2439
Fragments arranged by size:
4422 2439 248
KpnI G_GTAC'C
Cuts at: 3919 4129 3919
Size: 210 6899
MfeI C'AATT_G
Cuts at: 350 1177 2297 2381 350
Size: 827 1120 84 5078
Fragments arranged by size:
5078 1120 827 84
MluI A'CGCG_T
Cuts at: 6542 6542
Size: 7109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2172 3295 5868 6052 2172
Size: 1123 2573 184 3229
Fragments arranged by size:
3229 2573 1123 184
MscI TGG'CCA
Cuts at: 1048 1529 4870 1048
Size: 481 3341 3287
Fragments arranged by size:
3341 3287 481
MslI CAynn'nnrTG
Cuts at: 595 772 1634 1952 2204 5098 5225 595
Size: 177 862 318 252 2894 127 2479
Fragments arranged by size:
2894 2479 862 318 252 177 127
MspA1I CmG'CkG
Cuts at: 270 523 944 2463 4220 4534 4894 5678 5923
Size: 253 421 1519 1757 314 360 784 245
Cuts at: 5923 270
Size: 1456
Fragments arranged by size:
1757 1519 1456 784 421 360 314 253
245
NaeI GCC'GGC
Cuts at: 5290 5290
Size: 7109
NarI GG'CG_CC
Cuts at: 118 4540 4788 118
Size: 4422 248 2439
Fragments arranged by size:
4422 2439 248
NcoI C'CATG_G
Cuts at: 3089 3089
Size: 7109
NdeI CA'TA_TG
Cuts at: 1949 1949
Size: 7109
NgoMIV G'CCGG_C
Cuts at: 5288 5288
Size: 7109
NsiI A_TGCA'T
Cuts at: 289 1641 2617 289
Size: 1352 976 4781
Fragments arranged by size:
4781 1352 976
NspI r_CATG'y
Cuts at: 633 1190 1639 4162 4662 6265 633
Size: 557 449 2523 500 1603 1477
Fragments arranged by size:
2523 1603 1477 557 500 449
PciI A'CATG_T
Cuts at: 629 6261 629
Size: 5632 1477
PfoI T'CCnGG_A
Cuts at: 3213 3213
Size: 7109
PmeI GTTT'AAAC
Cuts at: 4108 4108
Size: 7109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5555 5587 3662
Size: 32 1861 32 5184
Fragments arranged by size:
5184 1861 32 32
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 7109
PstI C_TGCA'G
Cuts at: 1059 2783 4156 4841 1059
Size: 1724 1373 685 3327
Fragments arranged by size:
3327 1724 1373 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7109
PvuII CAG'CTG
Cuts at: 270 523 944 2463 4534 4894 270
Size: 253 421 1519 2071 360 2485
Fragments arranged by size:
2485 2071 1519 421 360 253
RsrII CG'GwC_CG
Cuts at: 5304 5304
Size: 7109
SacI G_AGCT'C
Cuts at: 4123 4123
Size: 7109
SalI G'TCGA_C
Cuts at: 1295 4146 1295
Size: 2851 4258
SapI GCTCTTCn'nnn_
Cuts at: 248 959 3524 5132 5342 248
Size: 711 2565 1608 210 2015
Fragments arranged by size:
2565 2015 1608 711 210
SbfI CC_TGCA'GG
Cuts at: 4156 4156
Size: 7109
ScaI AGT'ACT
Cuts at: 1746 2654 6626 1746
Size: 908 3972 2229
Fragments arranged by size:
3972 2229 908
SfcI C'TryA_G
Cuts at: 33 386 1055 1916 2779 4152 4837 5805 5996
Size: 353 669 861 863 1373 685 968 191
Cuts at: 5996 33
Size: 1146
Fragments arranged by size:
1373 1146 968 863 861 685 669 353
191
SfoI GGC'GCC
Cuts at: 119 4541 4789 119
Size: 4422 248 2439
Fragments arranged by size:
4422 2439 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7109
SmaI CCC'GGG
Cuts at: 4131 4131
Size: 7109
SmlI C'TyrA_G
Cuts at: 2857 4264 5179 5893 6155 2857
Size: 1407 915 714 262 3811
Fragments arranged by size:
3811 1407 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7109
SphI G_CATG'C
Cuts at: 1639 4162 4662 1639
Size: 2523 500 4086
Fragments arranged by size:
4086 2523 500
SspI AAT'ATT
Cuts at: 1676 2355 2910 1676
Size: 679 555 5875
Fragments arranged by size:
5875 679 555
StuI AGG'CCT
Cuts at: 3225 3225
Size: 7109
StyI C'CwwG_G
Cuts at: 298 1251 3089 3184 3593 3910 3988 4243 4597
Size: 953 1838 95 409 317 78 255 354
Cuts at: 4597 298
Size: 2810
Fragments arranged by size:
2810 1838 953 409 354 317 255 95
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 362 1280 2935 3287 4692 4825 5284 5493 362
Size: 918 1655 352 1405 133 459 209 1978
Fragments arranged by size:
1978 1655 1405 918 459 352 209 133
TatI w'GTAC_w
Cuts at: 504 1744 2154 2652 4064 6624 504
Size: 1240 410 498 1412 2560 989
Fragments arranged by size:
2560 1412 1240 989 498 410
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1060 1092 1904 1936 3891 3923 6626 6658 1060
Size: 32 812 32 1955 32 2703 32 1511
Fragments arranged by size:
2703 1955 1511 812 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4906 4906
Size: 7109
XbaI T'CTAG_A
Cuts at: 1749 4140 1749
Size: 2391 4718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 356 2384 4604 356
Size: 2028 2220 2861
Fragments arranged by size:
2861 2220 2028
XmaI C'CCGG_G
Cuts at: 4129 4129
Size: 7109
XmnI GAAnn'nnTTC
Cuts at: 788 2701 788
Size: 1913 5196
ZraI GAC'GTC
Cuts at: 1294 1294
Size: 7109
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AleI AloI ApaI AscI BaeI
BbvCI BmtI BsiWI BsmI BsmBI BspEI BssHII FseI
FspAI NheI NotI NruI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI