Restriction map for
pF1KB7217
(Circular) (Six-base) MAPSORT of: pF1KB7217.seq Check: 8331 from: 1 to: 4091
pF1KB7217 4091 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 890 890
Size: 4091
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 403 1916 2348 3095 3363 190
Size: 213 1513 432 747 268 918
Fragments arranged by size:
1513 918 747 432 268 213
AflIII A'CryG_T
Cuts at: 982 995 1443 2547 3524 982
Size: 13 448 1104 977 1549
Fragments arranged by size:
1549 1104 977 448 13
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AleI CACnn'nnGTG
Cuts at: 983 983
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 1396 2963 1396
Size: 1567 2524
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 486 1032 1095 3444 3700 486
Size: 546 63 2349 256 877
Fragments arranged by size:
2349 877 546 256 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 1111 1111
Size: 4091
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 1107 1521 1769 1804 1107
Size: 414 248 35 3394
Fragments arranged by size:
3394 414 248 35
BanII G_rGCy'C
Cuts at: 1105 2135 1105
Size: 1030 3061
BbeI G_GCGC'C
Cuts at: 1525 1773 1525
Size: 248 3843
BbsI GAAGACnn'nnnn_
Cuts at: 565 565
Size: 4091
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 492 1611 492
Size: 1119 2972
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 1716 1809 2865 1716
Size: 93 1056 2942
Fragments arranged by size:
2942 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2458 2458
Size: 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 272 3795 272
Size: 3523 568
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 603 1605 3642 6
Size: 597 1002 2037 455
Fragments arranged by size:
2037 1002 597 455
BsaHI Gr'CG_yC
Cuts at: 1522 1770 2472 3968 1522
Size: 248 702 1496 1645
Fragments arranged by size:
1645 1496 702 248
BsaWI w'CCGG_w
Cuts at: 263 1280 1801 2479 2753 2900 3311 263
Size: 1017 521 678 274 147 411 1043
Fragments arranged by size:
1043 1017 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 260 263 260
Size: 3 4088
BseYI C'CCAG_C
Cuts at: 1516 2274 2851 3347 1516
Size: 758 577 496 2260
Fragments arranged by size:
2260 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 417 1105 1883 2073 2865 417
Size: 688 778 190 792 1643
Fragments arranged by size:
1643 792 778 688 190
BsmI GAATG_Cn'
Cuts at: 131 131
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 417 1105 1716 1809 1883 2073 2135 2865 417
Size: 688 611 93 74 190 62 730 1643
Fragments arranged by size:
1643 730 688 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 103 890 1141 1355 1657 2038 103
Size: 787 251 214 302 381 2156
Fragments arranged by size:
2156 787 381 302 251 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 235 2003 235
Size: 1768 2323
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 534 2452 534
Size: 1918 2173
BstXI CCAn_nnnn'nTGG
Cuts at: 554 554
Size: 4091
BstYI r'GATC_y
Cuts at: 1 1116 1583 1606 1941 2187 3199 3285 3297
Size: 1115 467 23 335 246 1012 86 12
Cuts at: 3297 3643 1
Size: 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 386 700 107
Size: 279 314 3498
Fragments arranged by size:
3498 314 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 892 915 1334 892
Size: 23 419 3649
Fragments arranged by size:
3649 419 23
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 1005 1676 1850 2241 2268 3613 1005
Size: 671 174 391 27 1345 1483
Fragments arranged by size:
1483 1345 671 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 353 422 2114 2324 353
Size: 69 1692 210 2120
Fragments arranged by size:
2120 1692 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1103 1103
Size: 4091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 403 1916 2348 3095 3363 190
Size: 213 1513 432 747 268 918
Fragments arranged by size:
1513 918 747 432 268 213
EcoO109I rG'GnC_Cy
Cuts at: 115 1230 115
Size: 1115 2976
EcoRI G'AATT_C
Cuts at: 486 1095 486
Size: 609 3482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 1525 1773 2795 3774 1525
Size: 248 1022 979 1842
Fragments arranged by size:
1842 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 1933 1963 1965 1995 250
Size: 32 1651 30 2 30 2346
Fragments arranged by size:
2346 1651 32 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 1521 1769 1521
Size: 248 3843
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 1007 1852 1007
Size: 845 3246
MslI CAynn'nnrTG
Cuts at: 347 461 850 983 2080 2207 347
Size: 114 389 133 1097 127 2231
Fragments arranged by size:
2231 1097 389 133 127 114
MspA1I CmG'CkG
Cuts at: 140 1202 1516 1876 2889 3134 140
Size: 1062 314 360 1013 245 1097
Fragments arranged by size:
1097 1062 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 1522 1770 1522
Size: 248 3843
NcoI C'CATG_G
Cuts at: 107 386 700 107
Size: 279 314 3498
Fragments arranged by size:
3498 314 279
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 157 648 986 999 1144 1644 2551 157
Size: 491 338 13 145 500 907 1697
Fragments arranged by size:
1697 907 500 491 338 145 13
PciI A'CATG_T
Cuts at: 982 995 2547 982
Size: 13 1552 2526
Fragments arranged by size:
2526 1552 13
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 115 115
Size: 4091
PstI C_TGCA'G
Cuts at: 887 1138 1823 887
Size: 251 685 3155
Fragments arranged by size:
3155 685 251
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1516 1876 1516
Size: 360 3731
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 1105 1105
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 422 2114 2324 422
Size: 1692 210 2189
Fragments arranged by size:
2189 1692 210
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 804 883 1134 1819 2812 3003 33
Size: 771 79 251 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 251 191 79
SfoI GGC'GCC
Cuts at: 1523 1771 1523
Size: 248 3843
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 107 386 700 1225 1579 107
Size: 279 314 525 354 2619
Fragments arranged by size:
2619 525 354 314 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 424 503 889 3606 424
Size: 79 386 2717 909
Fragments arranged by size:
2717 909 386 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 508 1586 508
Size: 1078 3013
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI