Restriction map for
pF1KB7208
(Circular) (Six-base) MAPSORT of: pF1KB7208.seq Check: 6463 from: 1 to: 3431
pF1KB7208 3431 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 469 469
Size: 3431
Acc65I G'GTAC_C
Cuts at: 447 447
Size: 3431
AclI AA'CG_TT
Cuts at: 3271 3271
Size: 3431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1256 1688 2435 2703 277
Size: 979 432 747 268 1005
Fragments arranged by size:
1005 979 747 432 268
AflIII A'CryG_T
Cuts at: 136 783 1887 2864 136
Size: 647 1104 977 703
Fragments arranged by size:
1104 977 703 647
AgeI A'CCGG_T
Cuts at: 1819 1819
Size: 3431
AlwNI CAG_nnn'CTG
Cuts at: 189 736 2303 189
Size: 547 1567 1317
Fragments arranged by size:
1567 1317 547
ApaLI G'TGCA_C
Cuts at: 2201 2201
Size: 3431
ApoI r'AATT_y
Cuts at: 435 2784 3040 435
Size: 2349 256 826
Fragments arranged by size:
2349 826 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3431
AvaI C'yCGr_G
Cuts at: 336 451 336
Size: 115 3316
BamHI G'GATC_C
Cuts at: 456 456
Size: 3431
BanI G'GyrC_C
Cuts at: 447 861 1109 1144 447
Size: 414 248 35 2734
Fragments arranged by size:
2734 414 248 35
BanII G_rGCy'C
Cuts at: 445 1475 445
Size: 1030 2401
BbeI G_GCGC'C
Cuts at: 865 1113 865
Size: 248 3183
BciVI GTATCCnnnnn_n'
Cuts at: 1329 2090 3431 1329
Size: 761 1341 1329
Fragments arranged by size:
1341 1329 761
BclI T'GATC_A
Cuts at: 951 951
Size: 3431
BglII A'GATC_T
Cuts at: 946 2983 946
Size: 2037 1394
BlpI GC'TnA_GC
Cuts at: 543 543
Size: 3431
Bme1580I G_kGCm'C
Cuts at: 1056 1149 2205 1056
Size: 93 1056 2282
Fragments arranged by size:
2282 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 643 724 1059 3203 643
Size: 81 335 2144 871
Fragments arranged by size:
2144 871 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1486 1978 2276 607
Size: 879 492 298 1762
Fragments arranged by size:
1762 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3135 3135
Size: 3431
BsaAI yAC'GTr
Cuts at: 139 784 139
Size: 645 2786
BsaBI GATnn'nnATC
Cuts at: 6 945 2982 6
Size: 939 2037 455
Fragments arranged by size:
2037 939 455
BsaHI Gr'CG_yC
Cuts at: 862 1110 1812 3308 862
Size: 248 702 1496 985
Fragments arranged by size:
1496 985 702 248
BsaWI w'CCGG_w
Cuts at: 620 1141 1819 2093 2240 2651 620
Size: 521 678 274 147 411 1400
Fragments arranged by size:
1400 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 364 856 1614 2191 2687 364
Size: 492 758 577 496 1108
Fragments arranged by size:
1108 758 577 496 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 161
Size: 3431
BsiEI CG_ry'CG
Cuts at: 104 973 1019 2227 104
Size: 869 46 1208 1308
Fragments arranged by size:
1308 1208 869 46
BsiHKAI G_wGCw'C
Cuts at: 445 1223 1413 2205 445
Size: 778 190 792 1671
Fragments arranged by size:
1671 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 445 1056 1149 1223 1413 1475 2205 445
Size: 611 93 74 190 62 730 1671
Fragments arranged by size:
1671 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2651 2651
Size: 3431
BspHI T'CATG_A
Cuts at: 206 2607 206
Size: 2401 1030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 695 997 1378 481
Size: 214 302 381 2534
Fragments arranged by size:
2534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1723 1777 3261 3426 50
Size: 1673 54 1484 165 55
Fragments arranged by size:
1673 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1343 1343
Size: 3431
BsrFI r'CCGG_y
Cuts at: 1429 1610 1819 1429
Size: 181 209 3041
Fragments arranged by size:
3041 209 181
BssSI C'ACGA_G
Cuts at: 1702 2060 1702
Size: 358 3073
BstAPI GCAn_nnn'nTGC
Cuts at: 258 808 258
Size: 550 2881
BstBI TT'CG_AA
Cuts at: 1792 1792
Size: 3431
BstYI r'GATC_y
Cuts at: 1 456 923 946 1281 1527 2539 2625 2637
Size: 455 467 23 335 246 1012 86 12
Cuts at: 2637 2983 1
Size: 346 449
Fragments arranged by size:
1012 467 455 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1381 1562 1725 1381
Size: 181 163 3087
Fragments arranged by size:
3087 181 163
BtsI GCAGTG_nn'
Cuts at: 674 674
Size: 3431
DraI TTT'AAA
Cuts at: 430 430
Size: 3431
DrdI GACnn_nn'nnGTC
Cuts at: 1137 1995 1137
Size: 858 2573
EaeI y'GGCC_r
Cuts at: 1016 1190 1581 1608 2953 1016
Size: 174 391 27 1345 1494
Fragments arranged by size:
1494 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1016 1016
Size: 3431
EarI CTCTTCn'nnn_
Cuts at: 356 1454 1664 356
Size: 1098 210 2123
Fragments arranged by size:
2123 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1639 1949 2095 3241 1639
Size: 310 146 1146 1829
Fragments arranged by size:
1829 1146 310 146
EcoICRI GAG'CTC
Cuts at: 443 443
Size: 3431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1256 1688 2435 2703 277
Size: 979 432 747 268 1005
Fragments arranged by size:
1005 979 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 570 570
Size: 3431
EcoRI G'AATT_C
Cuts at: 435 435
Size: 3431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 367 1450 367
Size: 1083 2348
FspI TGC'GCA
Cuts at: 1212 1212
Size: 3431
HaeII r_GCGC'y
Cuts at: 865 1113 2135 3114 865
Size: 248 1022 979 1182
Fragments arranged by size:
1182 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 1273 1303 1305 1335 142
Size: 32 1099 30 2 30 2238
Fragments arranged by size:
2238 1099 32 30 30 2
HincII GTy'rAC
Cuts at: 470 470
Size: 3431
KasI G'GCGC_C
Cuts at: 861 1109 861
Size: 248 3183
KpnI G_GTAC'C
Cuts at: 451 451
Size: 3431
MluI A'CGCG_T
Cuts at: 2864 2864
Size: 3431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2102 2286 2102
Size: 184 3247
MscI TGG'CCA
Cuts at: 1192 1192
Size: 3431
MslI CAynn'nnrTG
Cuts at: 373 1420 1547 373
Size: 1047 127 2257
Fragments arranged by size:
2257 1047 127
MspA1I CmG'CkG
Cuts at: 542 856 1216 2229 2474 542
Size: 314 360 1013 245 1499
Fragments arranged by size:
1499 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1612 1612
Size: 3431
NarI GG'CG_CC
Cuts at: 862 1110 862
Size: 248 3183
NgoMIV G'CCGG_C
Cuts at: 1610 1610
Size: 3431
NspI r_CATG'y
Cuts at: 484 984 1891 484
Size: 500 907 2024
Fragments arranged by size:
2024 907 500
PciI A'CATG_T
Cuts at: 1887 1887
Size: 3431
PmeI GTTT'AAAC
Cuts at: 430 430
Size: 3431
PmlI CAC'GTG
Cuts at: 139 139
Size: 3431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2570 2602 2570
Size: 32 3399
PshAI GACnn'nnGTC
Cuts at: 118 118
Size: 3431
PstI C_TGCA'G
Cuts at: 478 1163 478
Size: 685 2746
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3431
PvuII CAG'CTG
Cuts at: 856 1216 856
Size: 360 3071
RsrII CG'GwC_CG
Cuts at: 1626 1626
Size: 3431
SacI G_AGCT'C
Cuts at: 445 445
Size: 3431
SalI G'TCGA_C
Cuts at: 468 468
Size: 3431
SapI GCTCTTCn'nnn_
Cuts at: 1454 1664 1454
Size: 210 3221
SbfI CC_TGCA'GG
Cuts at: 478 478
Size: 3431
ScaI AGT'ACT
Cuts at: 2948 2948
Size: 3431
SfcI C'TryA_G
Cuts at: 33 474 1159 2152 2343 33
Size: 441 685 993 191 1121
Fragments arranged by size:
1121 993 685 441 191
SfoI GGC'GCC
Cuts at: 863 1111 863
Size: 248 3183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3431
SmaI CCC'GGG
Cuts at: 338 453 338
Size: 115 3316
SmlI C'TyrA_G
Cuts at: 586 1501 1993 2255 586
Size: 915 492 262 1762
Fragments arranged by size:
1762 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3431
SphI G_CATG'C
Cuts at: 484 984 484
Size: 500 2931
SspI AAT'ATT
Cuts at: 246 246
Size: 3431
StyI C'CwwG_G
Cuts at: 565 919 565
Size: 354 3077
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1014 1147 1606 1815 1014
Size: 133 459 209 2630
Fragments arranged by size:
2630 459 209 133
TatI w'GTAC_w
Cuts at: 2946 2946
Size: 3431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3399
Tth111I GACn'n_nGTC
Cuts at: 1228 1228
Size: 3431
XbaI T'CTAG_A
Cuts at: 462 462
Size: 3431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 926 926
Size: 3431
XmaI C'CCGG_G
Cuts at: 336 451 336
Size: 115 3316
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PmlI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI