Restriction map for
pF1KB7207
(Circular) (Six-base) MAPSORT of: pF1KB7207.seq Check: 52 from: 1 to: 3782
pF1KB7207 3782 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 389 820 389
Size: 431 3351
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 542 1607 2039 2786 3054 362
Size: 180 1065 432 747 268 1090
Fragments arranged by size:
1090 1065 747 432 268 180
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 1087 2654 1087
Size: 1567 2215
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 786 3135 3391 786
Size: 2349 256 1177
Fragments arranged by size:
2349 1177 256
AseI AT'TA_AT
Cuts at: 20 707 20
Size: 687 3095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 802 802
Size: 3782
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 798 1212 1460 1495 798
Size: 414 248 35 3085
Fragments arranged by size:
3085 414 248 35
BanII G_rGCy'C
Cuts at: 796 1826 796
Size: 1030 2752
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BbsI GAAGACnn'nnnn_
Cuts at: 426 426
Size: 3782
BciVI GTATCCnnnnn_n'
Cuts at: 398 1680 2441 3782 398
Size: 1282 761 1341 398
Fragments arranged by size:
1341 1282 761 398
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 1407 1500 2556 1407
Size: 93 1056 2633
Fragments arranged by size:
2633 1056 93
BmgBI CAC'GTC
Cuts at: 133 133
Size: 3782
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 112 1207 1965 2542 3038 112
Size: 1095 758 577 496 856
Fragments arranged by size:
1095 856 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1574 1764 2556 796
Size: 778 190 792 2022
Fragments arranged by size:
2022 792 778 190
BsmI GAATG_Cn'
Cuts at: 323 323
Size: 3782
Bsp1286I G_dGCh'C
Cuts at: 796 1407 1500 1574 1764 1826 2556 796
Size: 611 93 74 190 62 730 2022
Fragments arranged by size:
2022 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstYI r'GATC_y
Cuts at: 1 807 1274 1297 1632 1878 2890 2976 2988
Size: 806 467 23 335 246 1012 86 12
Cuts at: 2988 3334 1
Size: 346 449
Fragments arranged by size:
1012 806 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 390 390
Size: 3782
BtgI C'CryG_G
Cuts at: 107 107
Size: 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 1367 1541 1932 1959 3304 1367
Size: 174 391 27 1345 1845
Fragments arranged by size:
1845 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 120 587 674 683 1805 2015 120
Size: 467 87 9 1122 210 1887
Fragments arranged by size:
1887 1122 467 210 87 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 542 1607 2039 2786 3054 362
Size: 180 1065 432 747 268 1090
Fragments arranged by size:
1090 1065 747 432 268 180
EcoO109I rG'GnC_Cy
Cuts at: 921 921
Size: 3782
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 338 1801 338
Size: 1463 2319
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 1216 1464 2486 3465 1216
Size: 248 1022 979 1533
Fragments arranged by size:
1533 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1624 1654 1656 1686 1624
Size: 30 2 30 3720
Fragments arranged by size:
3720 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 2453 2637 144
Size: 2309 184 1289
Fragments arranged by size:
2309 1289 184
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 1771 1898 1771
Size: 127 3655
MspA1I CmG'CkG
Cuts at: 893 1207 1567 2580 2825 893
Size: 314 360 1013 245 1850
Fragments arranged by size:
1850 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NcoI C'CATG_G
Cuts at: 107 107
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PsiI TTA'TAA
Cuts at: 236 236
Size: 3782
PstI C_TGCA'G
Cuts at: 829 1514 829
Size: 685 3097
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 1207 1567 1207
Size: 360 3422
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 674 1805 2015 674
Size: 1131 210 2441
Fragments arranged by size:
2441 1131 210
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 825 1510 2503 2694 33
Size: 792 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 191
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
SspI AAT'ATT
Cuts at: 153 153
Size: 3782
StyI C'CwwG_G
Cuts at: 107 916 1270 107
Size: 809 354 2619
Fragments arranged by size:
2619 809 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 3297 3297
Size: 3782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 1277
Size: 3782
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI