Restriction map for
pF1KB7200
(Circular) (Six-base) MAPSORT of: pF1KB7200.seq Check: 4036 from: 1 to: 4406
pF1KB7200 4406 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 453 1364 453
Size: 911 3495
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1212 2231 2663 3410 3678 1212
Size: 1019 432 747 268 1940
Fragments arranged by size:
1940 1019 747 432 268
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 627 627
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 831 843 1711 3278 831
Size: 12 868 1567 1959
Fragments arranged by size:
1959 1567 868 12
ApaI G_GGCC'C
Cuts at: 222 229 270 370 222
Size: 7 41 100 4258
Fragments arranged by size:
4258 100 41 7
ApaLI G'TGCA_C
Cuts at: 466 632 3176 466
Size: 166 2544 1696
Fragments arranged by size:
2544 1696 166
ApoI r'AATT_y
Cuts at: 1410 3759 4015 1410
Size: 2349 256 1801
Fragments arranged by size:
2349 1801 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 221 1001 1426 221
Size: 780 425 3201
Fragments arranged by size:
3201 780 425
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 345 1037 1422 1836 2084 2119 345
Size: 692 385 414 248 35 2632
Fragments arranged by size:
2632 692 414 385 248 35
BanII G_rGCy'C
Cuts at: 222 229 270 370 484 676 943 1420 2450
Size: 7 41 100 114 192 267 477 1030
Cuts at: 2450 222
Size: 2178
Fragments arranged by size:
2178 1030 477 267 192 114 100 41
7
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BbsI GAAGACnn'nnnn_
Cuts at: 519 720 519
Size: 201 4205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 491 1926 491
Size: 1435 2971
BglI GCCn_nnn'nGGC
Cuts at: 877 886 877
Size: 9 4397
BglII A'GATC_T
Cuts at: 252 1193 1921 3958 252
Size: 941 728 2037 700
Fragments arranged by size:
2037 941 728 700
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 222 229 270 360 370 470 636 668 798
Size: 7 41 90 10 100 166 32 130
Cuts at: 798 2031 2124 3180 222
Size: 1233 93 1056 1448
Fragments arranged by size:
1448 1233 1056 166 130 100 93 90
41 32 10 7
BmrI ACTGGGnnnn_n'
Cuts at: 530 623 1618 1699 2034 4178 530
Size: 93 995 81 335 2144 758
Fragments arranged by size:
2144 995 758 335 93 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 379 496 793 853 871 1151 1180 1360
Size: 175 117 297 60 18 280 29 180
Cuts at: 1360 204
Size: 3250
Fragments arranged by size:
3250 297 280 180 175 117 60 29
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 736 1582 2461 2953 3251 520
Size: 216 846 879 492 298 1675
Fragments arranged by size:
1675 879 846 492 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1595 2116 2794 3068 3215 3626 1031
Size: 564 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 564 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 374 404 788 818 925 955 374
Size: 30 384 30 107 30 3825
Fragments arranged by size:
3825 384 107 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 382 796 267
Size: 115 414 3877
Fragments arranged by size:
3877 414 115
BseYI C'CCAG_C
Cuts at: 178 213 363 483 889 984 1148 1369 1831
Size: 35 150 120 406 95 164 221 462
Cuts at: 1831 2589 3166 3662 178
Size: 758 577 496 922
Fragments arranged by size:
922 758 577 496 462 406 221 164
150 120 95 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 597 1264 176
Size: 421 667 3318
Fragments arranged by size:
3318 667 421
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 470 636 676 1420 2198 2388 3180 470
Size: 166 40 744 778 190 792 1696
Fragments arranged by size:
1696 792 778 744 190 166 40
Bsp1286I G_dGCh'C
Cuts at: 222 229 270 360 370 470 484 636 668
Size: 7 41 90 10 100 14 152 32
Cuts at: 668 676 798 943 1420 2031 2124 2198 2388
Size: 8 122 145 477 611 93 74 190
Cuts at: 2388 2450 3180 222
Size: 62 730 1448
Fragments arranged by size:
1448 730 611 477 190 152 145 122
100 93 90 74 62 41 32 14
10 8 7
BspEI T'CCGG_A
Cuts at: 1031 3626 1031
Size: 2595 1811
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 453 1364 1456 1670 1972 2353 218
Size: 235 911 92 214 302 381 2271
Fragments arranged by size:
2271 911 381 302 235 214 92
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2318 2318
Size: 4406
BsrFI r'CCGG_y
Cuts at: 600 2404 2585 2794 600
Size: 1804 181 209 2212
Fragments arranged by size:
2212 1804 209 181
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 846 1783 846
Size: 937 3469
BstBI TT'CG_AA
Cuts at: 731 2767 731
Size: 2036 2370
BstXI CCAn_nnnn'nTGG
Cuts at: 889 889
Size: 4406
BstYI r'GATC_y
Cuts at: 1 252 1193 1431 1898 1921 2256 2502 3514
Size: 251 941 238 467 23 335 246 1012
Cuts at: 3514 3600 3612 3958 1
Size: 86 12 346 449
Fragments arranged by size:
1012 941 467 449 346 335 251 246
238 86 23 12
BtgI C'CryG_G
Cuts at: 1053 1328 1053
Size: 275 4131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 290 2356 2537 2700 290
Size: 2066 181 163 1996
Fragments arranged by size:
2066 1996 181 163
BtsI GCAGTG_nn'
Cuts at: 325 604 682 756 1649 325
Size: 279 78 74 893 3082
Fragments arranged by size:
3082 893 279 78 74
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 572 1991 2165 2556 2583 3928 572
Size: 1419 174 391 27 1345 1050
Fragments arranged by size:
1419 1345 1050 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 285 728 2429 2639 285
Size: 443 1701 210 2052
Fragments arranged by size:
2052 1701 443 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 781 1252 2614 2924 3070 4216 781
Size: 471 1362 310 146 1146 971
Fragments arranged by size:
1362 1146 971 471 310 146
EcoICRI GAG'CTC
Cuts at: 674 1418 674
Size: 744 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 379 496 793 853 871 1151 1180 1212
Size: 175 117 297 60 18 280 29 32
Cuts at: 1212 1360 2231 2663 3410 3678 204
Size: 148 871 432 747 268 932
Fragments arranged by size:
932 871 747 432 297 280 268 175
148 117 60 32 29 18
EcoO109I rG'GnC_Cy
Cuts at: 218 225 266 367 973 1008 1545 218
Size: 7 41 101 606 35 537 3079
Fragments arranged by size:
3079 606 537 101 41 35 7
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 1840 2088 3110 4089 1840
Size: 248 1022 979 2157
Fragments arranged by size:
2157 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 925 957 2248 2278 2280 2310 925
Size: 32 1291 30 2 30 3021
Fragments arranged by size:
3021 1291 32 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 956 1406 3077 3261 956
Size: 450 1671 184 2101
Fragments arranged by size:
2101 1671 450 184
MscI TGG'CCA
Cuts at: 574 2167 574
Size: 1593 2813
MslI CAynn'nnrTG
Cuts at: 1213 2395 2522 1213
Size: 1182 127 3097
Fragments arranged by size:
3097 1182 127
MspA1I CmG'CkG
Cuts at: 182 312 318 843 1148 1179 1517 1831 2191
Size: 130 6 525 305 31 338 314 360
Cuts at: 2191 3204 3449 182
Size: 1013 245 1139
Fragments arranged by size:
1139 1013 525 360 338 314 305 245
130 31 6
NaeI GCC'GGC
Cuts at: 602 2587 602
Size: 1985 2421
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 1053 1053
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 600 2585 600
Size: 1985 2421
NspI r_CATG'y
Cuts at: 1116 1218 1459 1959 2866 1116
Size: 102 241 500 907 2656
Fragments arranged by size:
2656 907 500 241 102
PasI CC'CwG_GG
Cuts at: 229 229
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PflMI CCAn_nnn'nTGG
Cuts at: 777 1089 777
Size: 312 4094
PfoI T'CCnGG_A
Cuts at: 395 1157 395
Size: 762 3644
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 3545 3577 1327
Size: 32 2186 32 2156
Fragments arranged by size:
2186 2156 32 32
PspOMI G'GGCC_C
Cuts at: 218 225 266 366 218
Size: 7 41 100 4258
Fragments arranged by size:
4258 100 41 7
PstI C_TGCA'G
Cuts at: 317 464 1453 2138 317
Size: 147 989 685 2585
Fragments arranged by size:
2585 989 685 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 182 312 318 843 1831 2191 182
Size: 130 6 525 988 360 2397
Fragments arranged by size:
2397 988 525 360 130 6
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 676 1420 676
Size: 744 3662
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 728 2429 2639 728
Size: 1701 210 2495
Fragments arranged by size:
2495 1701 210
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 313 460 1449 2134 3127 3318 33
Size: 280 147 989 685 993 191 1121
Fragments arranged by size:
1121 993 989 685 280 191 147
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 223 1003 1428 223
Size: 780 425 3201
Fragments arranged by size:
3201 780 425
SmlI C'TyrA_G
Cuts at: 535 751 1561 2476 2968 3230 535
Size: 216 810 915 492 262 1711
Fragments arranged by size:
1711 915 810 492 262 216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StuI AGG'CCT
Cuts at: 123 123
Size: 4406
StyI C'CwwG_G
Cuts at: 146 153 908 969 1053 1540 1894 146
Size: 7 755 61 84 487 354 2658
Fragments arranged by size:
2658 755 487 354 84 61 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 210 522 576 651 1989 2122 2581 2790 210
Size: 312 54 75 1338 133 459 209 1826
Fragments arranged by size:
1826 1338 459 312 209 133 75 54
TatI w'GTAC_w
Cuts at: 3921 3921
Size: 4406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 788 820 3923 3955 788
Size: 32 3103 32 1239
Fragments arranged by size:
3103 1239 32 32
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 221 1001 1426 221
Size: 780 425 3201
Fragments arranged by size:
3201 780 425
XmnI GAAnn'nnTTC
Cuts at: 727 727
Size: 4406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI