Restriction map for
pF1KB7195
(Circular) (Six-base) MAPSORT of: pF1KB7195.seq Check: 8827 from: 1 to: 3500
pF1KB7195 3500 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 332 382 538 332
Size: 50 156 3294
Fragments arranged by size:
3294 156 50
Acc65I G'GTAC_C
Cuts at: 516 516
Size: 3500
AclI AA'CG_TT
Cuts at: 3340 3340
Size: 3500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 1757 2504 2772 1325
Size: 432 747 268 2053
Fragments arranged by size:
2053 747 432 268
AflIII A'CryG_T
Cuts at: 310 852 1956 2933 310
Size: 542 1104 977 877
Fragments arranged by size:
1104 977 877 542
AgeI A'CCGG_T
Cuts at: 1888 1888
Size: 3500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 174 174
Size: 3500
AlwNI CAG_nnn'CTG
Cuts at: 470 805 2372 470
Size: 335 1567 1598
Fragments arranged by size:
1598 1567 335
ApaI G_GGCC'C
Cuts at: 464 464
Size: 3500
ApaLI G'TGCA_C
Cuts at: 2270 2270
Size: 3500
ApoI r'AATT_y
Cuts at: 504 2853 3109 504
Size: 2349 256 895
Fragments arranged by size:
2349 895 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3500
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
AvaI C'yCGr_G
Cuts at: 520 520
Size: 3500
BamHI G'GATC_C
Cuts at: 525 525
Size: 3500
BanI G'GyrC_C
Cuts at: 516 930 1178 1213 516
Size: 414 248 35 2803
Fragments arranged by size:
2803 414 248 35
BanII G_rGCy'C
Cuts at: 464 514 1544 464
Size: 50 1030 2420
Fragments arranged by size:
2420 1030 50
BbeI G_GCGC'C
Cuts at: 934 1182 934
Size: 248 3252
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 3500
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2159 3500 1398
Size: 761 1341 1398
Fragments arranged by size:
1398 1341 761
BclI T'GATC_A
Cuts at: 1020 1020
Size: 3500
BglII A'GATC_T
Cuts at: 1015 3052 1015
Size: 2037 1463
BlpI GC'TnA_GC
Cuts at: 612 612
Size: 3500
Bme1580I G_kGCm'C
Cuts at: 353 464 1125 1218 2274 353
Size: 111 661 93 1056 1579
Fragments arranged by size:
1579 1056 661 111 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 396 712 793 1128 3272 385
Size: 11 316 81 335 2144 613
Fragments arranged by size:
2144 613 335 316 81 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 103
Size: 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1555 2047 2345 676
Size: 879 492 298 1831
Fragments arranged by size:
1831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3204 3204
Size: 3500
BsaAI yAC'GTr
Cuts at: 853 853
Size: 3500
BsaBI GATnn'nnATC
Cuts at: 6 1014 3051 6
Size: 1008 2037 455
Fragments arranged by size:
2037 1008 455
BsaHI Gr'CG_yC
Cuts at: 931 1179 1881 3377 931
Size: 248 702 1496 1054
Fragments arranged by size:
1496 1054 702 248
BsaWI w'CCGG_w
Cuts at: 689 1210 1888 2162 2309 2720 689
Size: 521 678 274 147 411 1469
Fragments arranged by size:
1469 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 285 463 925 1683 2260 2756 285
Size: 178 462 758 577 496 1029
Fragments arranged by size:
1029 758 577 496 462 178
BsiEI CG_ry'CG
Cuts at: 104 1042 1088 2296 104
Size: 938 46 1208 1308
Fragments arranged by size:
1308 1208 938 46
BsiHKAI G_wGCw'C
Cuts at: 363 514 1292 1482 2274 363
Size: 151 778 190 792 1589
Fragments arranged by size:
1589 792 778 190 151
Bsp1286I G_dGCh'C
Cuts at: 353 363 464 514 1125 1218 1292 1482 1544
Size: 10 101 50 611 93 74 190 62
Cuts at: 1544 2274 353
Size: 730 1579
Fragments arranged by size:
1579 730 611 190 101 93 74 62
50 10
BspEI T'CCGG_A
Cuts at: 2720 2720
Size: 3500
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 550 764 1066 1447 550
Size: 214 302 381 2603
Fragments arranged by size:
2603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1792 1846 3330 3495 50
Size: 1742 54 1484 165 55
Fragments arranged by size:
1742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 188 1412 188
Size: 1224 2276
BsrFI r'CCGG_y
Cuts at: 1498 1679 1888 1498
Size: 181 209 3110
Fragments arranged by size:
3110 209 181
BssSI C'ACGA_G
Cuts at: 1771 2129 1771
Size: 358 3142
BstAPI GCAn_nnn'nTGC
Cuts at: 877 877
Size: 3500
BstBI TT'CG_AA
Cuts at: 1861 1861
Size: 3500
BstXI CCAn_nnnn'nTGG
Cuts at: 270 270
Size: 3500
BstYI r'GATC_y
Cuts at: 1 267 525 992 1015 1350 1596 2608 2694
Size: 266 258 467 23 335 246 1012 86
Cuts at: 2694 2706 3052 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 266 258 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 333 383 333
Size: 50 3450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1450 1631 1794 1450
Size: 181 163 3156
Fragments arranged by size:
3156 181 163
BtsI GCAGTG_nn'
Cuts at: 743 743
Size: 3500
DraI TTT'AAA
Cuts at: 499 499
Size: 3500
DraIII CAC_nnn'GTG
Cuts at: 242 404 242
Size: 162 3338
DrdI GACnn_nn'nnGTC
Cuts at: 1206 2064 1206
Size: 858 2642
EaeI y'GGCC_r
Cuts at: 395 1085 1259 1650 1677 3022 395
Size: 690 174 391 27 1345 873
Fragments arranged by size:
1345 873 690 391 174 27
EagI C'GGCC_G
Cuts at: 1085 1085
Size: 3500
EarI CTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1708 2018 2164 3310 1708
Size: 310 146 1146 1898
Fragments arranged by size:
1898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 512 512
Size: 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 1325 1757 2504 2772 103
Size: 1222 432 747 268 831
Fragments arranged by size:
1222 831 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 282 460 639 282
Size: 178 179 3143
Fragments arranged by size:
3143 179 178
EcoRI G'AATT_C
Cuts at: 504 504
Size: 3500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1519 1519
Size: 3500
FspI TGC'GCA
Cuts at: 1281 1281
Size: 3500
HaeII r_GCGC'y
Cuts at: 934 1182 2204 3183 934
Size: 248 1022 979 1251
Fragments arranged by size:
1251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1342 1372 1374 1404 1342
Size: 30 2 30 3438
Fragments arranged by size:
3438 30 30 2
HincII GTy'rAC
Cuts at: 539 539
Size: 3500
KasI G'GCGC_C
Cuts at: 930 1178 930
Size: 248 3252
KpnI G_GTAC'C
Cuts at: 520 520
Size: 3500
MluI A'CGCG_T
Cuts at: 2933 2933
Size: 3500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2355 2171
Size: 184 3316
MscI TGG'CCA
Cuts at: 397 1261 397
Size: 864 2636
MslI CAynn'nnrTG
Cuts at: 1489 1616 1489
Size: 127 3373
MspA1I CmG'CkG
Cuts at: 611 925 1285 2298 2543 611
Size: 314 360 1013 245 1568
Fragments arranged by size:
1568 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1681 1681
Size: 3500
NarI GG'CG_CC
Cuts at: 931 1179 931
Size: 248 3252
NgoMIV G'CCGG_C
Cuts at: 1679 1679
Size: 3500
NspI r_CATG'y
Cuts at: 314 553 1053 1960 314
Size: 239 500 907 1854
Fragments arranged by size:
1854 907 500 239
PciI A'CATG_T
Cuts at: 310 1956 310
Size: 1646 1854
PmeI GTTT'AAAC
Cuts at: 499 499
Size: 3500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2639 2671 2639
Size: 32 3468
PpuMI rG'GwC_Cy
Cuts at: 282 282
Size: 3500
PspOMI G'GGCC_C
Cuts at: 460 460
Size: 3500
PstI C_TGCA'G
Cuts at: 547 1232 547
Size: 685 2815
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3500
PvuII CAG'CTG
Cuts at: 925 1285 925
Size: 360 3140
RsrII CG'GwC_CG
Cuts at: 1695 1695
Size: 3500
SacI G_AGCT'C
Cuts at: 514 514
Size: 3500
SalI G'TCGA_C
Cuts at: 537 537
Size: 3500
SapI GCTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
SbfI CC_TGCA'GG
Cuts at: 547 547
Size: 3500
ScaI AGT'ACT
Cuts at: 3017 3017
Size: 3500
SfcI C'TryA_G
Cuts at: 33 543 1228 2221 2412 33
Size: 510 685 993 191 1121
Fragments arranged by size:
1121 993 685 510 191
SfoI GGC'GCC
Cuts at: 932 1180 932
Size: 248 3252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
SmaI CCC'GGG
Cuts at: 522 522
Size: 3500
SmlI C'TyrA_G
Cuts at: 655 1570 2062 2324 655
Size: 915 492 262 1831
Fragments arranged by size:
1831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3500
SphI G_CATG'C
Cuts at: 553 1053 553
Size: 500 3000
StuI AGG'CCT
Cuts at: 455 455
Size: 3500
StyI C'CwwG_G
Cuts at: 634 988 634
Size: 354 3146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 415 1083 1216 1675 1884 415
Size: 668 133 459 209 2031
Fragments arranged by size:
2031 668 459 209 133
TatI w'GTAC_w
Cuts at: 442 3015 442
Size: 2573 927
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3468
Tth111I GACn'n_nGTC
Cuts at: 1297 1297
Size: 3500
XbaI T'CTAG_A
Cuts at: 531 531
Size: 3500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 995 409
Size: 586 2914
XmaI C'CCGG_G
Cuts at: 520 520
Size: 3500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII Bsu36I BtgI ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI