Restriction map for
pF1KB7184
(Circular) (Six-base) MAPSORT of: pF1KB7184.seq Check: 3585 from: 1 to: 3776
pF1KB7184 3776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 814 814
Size: 3776
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 537 1601 2033 2780 3048 394
Size: 143 1064 432 747 268 1122
Fragments arranged by size:
1122 1064 747 432 268 143
AflIII A'CryG_T
Cuts at: 1128 2232 3209 1128
Size: 1104 977 1695
Fragments arranged by size:
1695 1104 977
AgeI A'CCGG_T
Cuts at: 2164 2164
Size: 3776
AlwNI CAG_nnn'CTG
Cuts at: 1081 2648 1081
Size: 1567 2209
ApaLI G'TGCA_C
Cuts at: 2546 2546
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 796 796
Size: 3776
BamHI G'GATC_C
Cuts at: 801 801
Size: 3776
BanI G'GyrC_C
Cuts at: 220 792 1206 1454 1489 220
Size: 572 414 248 35 2507
Fragments arranged by size:
2507 572 414 248 35
BanII G_rGCy'C
Cuts at: 464 790 1820 464
Size: 326 1030 2420
Fragments arranged by size:
2420 1030 326
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbsI GAAGACnn'nnnn_
Cuts at: 543 543
Size: 3776
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2435 3776 1674
Size: 761 1341 1674
Fragments arranged by size:
1674 1341 761
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglII A'GATC_T
Cuts at: 1291 3328 1291
Size: 2037 1739
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 601 1401 1494 2550 601
Size: 800 93 1056 1827
Fragments arranged by size:
1827 1056 800 93
BmrI ACTGGGnnnn_n'
Cuts at: 988 1069 1404 3548 988
Size: 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 346
Size: 3776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 952 1831 2323 2621 448
Size: 504 879 492 298 1603
Fragments arranged by size:
1603 879 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3480 3480
Size: 3776
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 1207 1455 2157 3653 1207
Size: 248 702 1496 1330
Fragments arranged by size:
1496 1330 702 248
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2438 2585 2996 965
Size: 521 678 274 147 411 1745
Fragments arranged by size:
1745 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 476 506 476
Size: 30 3746
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 3776
BseYI C'CCAG_C
Cuts at: 1201 1959 2536 3032 1201
Size: 758 577 496 1945
Fragments arranged by size:
1945 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1318 1364 2572 104
Size: 1214 46 1208 1308
Fragments arranged by size:
1308 1214 1208 46
BsiHKAI G_wGCw'C
Cuts at: 790 1568 1758 2550 790
Size: 778 190 792 2016
Fragments arranged by size:
2016 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 464 601 790 1401 1494 1568 1758 1820 2550
Size: 137 189 611 93 74 190 62 730
Cuts at: 2550 464
Size: 1690
Fragments arranged by size:
1690 730 611 190 189 137 93 74
62
BspEI T'CCGG_A
Cuts at: 2996 2996
Size: 3776
BspHI T'CATG_A
Cuts at: 2952 2952
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1040 1342 1723 826
Size: 214 302 381 2879
Fragments arranged by size:
2879 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2068 2122 3606 3771 50
Size: 2018 54 1484 165 55
Fragments arranged by size:
2018 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 1774 1955 2164 1774
Size: 181 209 3386
Fragments arranged by size:
3386 209 181
BssSI C'ACGA_G
Cuts at: 2047 2405 2047
Size: 358 3418
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1153
Size: 3776
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 801 1268 1291 1626 1872 2884 2970 2982
Size: 800 467 23 335 246 1012 86 12
Cuts at: 2982 3328 1
Size: 346 449
Fragments arranged by size:
1012 800 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 1726 1907 2070 228
Size: 1498 181 163 1934
Fragments arranged by size:
1934 1498 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1019
Size: 3776
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 1482 2340 1482
Size: 858 2918
EaeI y'GGCC_r
Cuts at: 314 1361 1535 1926 1953 3298 314
Size: 1047 174 391 27 1345 792
Fragments arranged by size:
1345 1047 792 391 174 27
EagI C'GGCC_G
Cuts at: 1361 1361
Size: 3776
EarI CTCTTCn'nnn_
Cuts at: 307 1799 2009 307
Size: 1492 210 2074
Fragments arranged by size:
2074 1492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2294 2440 3586 1984
Size: 310 146 1146 2174
Fragments arranged by size:
2174 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 394 537 1601 2033 2780 3048 346
Size: 48 143 1064 432 747 268 1074
Fragments arranged by size:
1074 1064 747 432 268 143 48
EcoO109I rG'GnC_Cy
Cuts at: 621 673 915 621
Size: 52 242 3482
Fragments arranged by size:
3482 242 52
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 469 1795 469
Size: 1326 2450
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 1210 1458 2480 3459 1210
Size: 248 1022 979 1527
Fragments arranged by size:
1527 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 309 815 309
Size: 506 3270
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 628 2447 2631 628
Size: 1819 184 1773
Fragments arranged by size:
1819 1773 184
MscI TGG'CCA
Cuts at: 316 1537 316
Size: 1221 2555
MslI CAynn'nnrTG
Cuts at: 1765 1892 1765
Size: 127 3649
MspA1I CmG'CkG
Cuts at: 887 1201 1561 2574 2819 887
Size: 314 360 1013 245 1844
Fragments arranged by size:
1844 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NspI r_CATG'y
Cuts at: 829 1329 2236 829
Size: 500 907 2369
Fragments arranged by size:
2369 907 500
PciI A'CATG_T
Cuts at: 2232 2232
Size: 3776
PfoI T'CCnGG_A
Cuts at: 362 362
Size: 3776
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2915 2947 2915
Size: 32 3744
PpuMI rG'GwC_Cy
Cuts at: 621 621
Size: 3776
PstI C_TGCA'G
Cuts at: 823 1508 823
Size: 685 3091
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3776
PvuII CAG'CTG
Cuts at: 1201 1561 1201
Size: 360 3416
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SalI G'TCGA_C
Cuts at: 813 813
Size: 3776
SanDI GG'GwC_CC
Cuts at: 621 621
Size: 3776
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 819 1504 2497 2688 33
Size: 786 685 993 191 1121
Fragments arranged by size:
1121 993 786 685 191
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 463 931 1846 2338 2600 463
Size: 468 915 492 262 1639
Fragments arranged by size:
1639 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 910 1264 910
Size: 354 3422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1359 1492 1951 2160 1359
Size: 133 459 209 2975
Fragments arranged by size:
2975 459 209 133
TatI w'GTAC_w
Cuts at: 3291 3291
Size: 3776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
XmnI GAAnn'nnTTC
Cuts at: 550 757 550
Size: 207 3569
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI