Restriction map for
pF1KB7181
(Circular) (Six-base) MAPSORT of: pF1KB7181.seq Check: 7974 from: 1 to: 4178
pF1KB7181 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 128 1174 128
Size: 1046 3132
AccI GT'mk_AC
Cuts at: 932 1216 932
Size: 284 3894
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2435 3182 3450 2003
Size: 432 747 268 2731
Fragments arranged by size:
2731 747 432 268
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 234 1483 3050 234
Size: 1249 1567 1362
Fragments arranged by size:
1567 1362 1249
ApaI G_GGCC'C
Cuts at: 416 416
Size: 4178
ApaLI G'TGCA_C
Cuts at: 1159 2948 1159
Size: 1789 2389
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 124 863 1198 124
Size: 739 335 3104
Fragments arranged by size:
3104 739 335
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 154 508 1194 1608 1856 1891 154
Size: 354 686 414 248 35 2441
Fragments arranged by size:
2441 686 414 354 248 35
BanII G_rGCy'C
Cuts at: 116 226 416 965 1192 2222 116
Size: 110 190 549 227 1030 2072
Fragments arranged by size:
2072 1030 549 227 190 110
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 766 766
Size: 4178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 276 310 276
Size: 34 4144
BciVI GTATCCnnnnn_n'
Cuts at: 2076 2837 4178 2076
Size: 761 1341 2076
Fragments arranged by size:
2076 1341 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglI GCCn_nnn'nGGC
Cuts at: 475 475
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 416 511 1163 1803 1896 2952 416
Size: 95 652 640 93 1056 1642
Fragments arranged by size:
1642 1056 652 640 95 93
BmgBI CAC'GTC
Cuts at: 1158 1158
Size: 4178
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 528 2545 528
Size: 2017 2161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1354 2233 2725 3023 793
Size: 561 879 492 298 1948
Fragments arranged by size:
1948 879 561 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 871 3882 871
Size: 3011 1167
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 998 1367 1888 2566 2840 2987 3398 998
Size: 369 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 928 958 928
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 124
Size: 4178
BseYI C'CCAG_C
Cuts at: 307 330 869 1603 2361 2938 3434 307
Size: 23 539 734 758 577 496 1051
Fragments arranged by size:
1051 758 734 577 539 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 363
Size: 4178
BsiEI CG_ry'CG
Cuts at: 104 470 1720 1766 2974 104
Size: 366 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 366 46
BsiHKAI G_wGCw'C
Cuts at: 1163 1192 1970 2160 2952 1163
Size: 29 778 190 792 2389
Fragments arranged by size:
2389 792 778 190 29
BsmBI CGTCTCn'nnnn_
Cuts at: 967 967
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 116 226 416 511 965 1163 1192 1803 1896
Size: 110 190 95 454 198 29 611 93
Cuts at: 1896 1970 2160 2222 2952 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 454 198 190 190 110
95 93 74 62 29
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 772 1174 1228 1442 1744 2125 128
Size: 644 402 54 214 302 381 2181
Fragments arranged by size:
2181 644 402 381 302 214 54
BsrBI CCG'CTC
Cuts at: 50 494 2470 2524 4008 4173 50
Size: 444 1976 54 1484 165 55
Fragments arranged by size:
1976 1484 444 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1014 2090 1014
Size: 1076 3102
BsrFI r'CCGG_y
Cuts at: 469 2176 2357 2566 469
Size: 1707 181 209 2081
Fragments arranged by size:
2081 1707 209 181
BssHII G'CGCG_C
Cuts at: 631 652 631
Size: 21 4157
BssSI C'ACGA_G
Cuts at: 1068 2449 2807 1068
Size: 1381 358 2439
Fragments arranged by size:
2439 1381 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 185 185
Size: 4178
BstYI r'GATC_y
Cuts at: 1 1203 1670 1693 2028 2274 3286 3372 3384
Size: 1202 467 23 335 246 1012 86 12
Cuts at: 3384 3730 1
Size: 346 449
Fragments arranged by size:
1202 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 732 914 1020 107
Size: 625 182 106 3265
Fragments arranged by size:
3265 625 182 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 444 2128 2309 2472 444
Size: 1684 181 163 2150
Fragments arranged by size:
2150 1684 181 163
BtsI GCAGTG_nn'
Cuts at: 1098 1421 1098
Size: 323 3855
ClaI AT'CG_AT
Cuts at: 1028 1028
Size: 4178
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 314 401 467 659 1053 1763 1937 2328 2355
Size: 87 66 192 394 710 174 391 27
Cuts at: 2355 3700 314
Size: 1345 792
Fragments arranged by size:
1345 792 710 394 391 192 174 87
66 27
EagI C'GGCC_G
Cuts at: 467 1763 467
Size: 1296 2882
EarI CTCTTCn'nnn_
Cuts at: 907 2201 2411 907
Size: 1294 210 2674
Fragments arranged by size:
2674 1294 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2435 3182 3450 2003
Size: 432 747 268 2731
Fragments arranged by size:
2731 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 557 557
Size: 4178
EcoO109I rG'GnC_Cy
Cuts at: 162 924 1317 162
Size: 762 393 3023
Fragments arranged by size:
3023 762 393
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4178
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 830 1612 1860 2882 3861 830
Size: 782 248 1022 979 1147
Fragments arranged by size:
1147 1022 979 782 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 814 846 2020 2050 2052 2082 640
Size: 32 142 32 1174 30 2 30 2736
Fragments arranged by size:
2736 1174 142 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 403 1939 403
Size: 1536 2642
MslI CAynn'nnrTG
Cuts at: 2167 2294 2167
Size: 127 4051
MspA1I CmG'CkG
Cuts at: 371 749 1130 1289 1603 1963 2976 3221 371
Size: 378 381 159 314 360 1013 245 1328
Fragments arranged by size:
1328 1013 381 378 360 314 245 159
NaeI GCC'GGC
Cuts at: 471 2359 471
Size: 1888 2290
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 469 2357 469
Size: 1888 2290
NspI r_CATG'y
Cuts at: 714 1231 1731 2638 714
Size: 517 500 907 2254
Fragments arranged by size:
2254 907 517 500
PasI CC'CwG_GG
Cuts at: 198 198
Size: 4178
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PfoI T'CCnGG_A
Cuts at: 338 701 338
Size: 363 3815
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 162 924 162
Size: 762 3416
PspOMI G'GGCC_C
Cuts at: 412 412
Size: 4178
PstI C_TGCA'G
Cuts at: 583 1225 1910 583
Size: 642 685 2851
Fragments arranged by size:
2851 685 642
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 749 1130 1603 1963 749
Size: 381 473 360 2964
Fragments arranged by size:
2964 473 381 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 907 2201 2411 907
Size: 1294 210 2674
Fragments arranged by size:
2674 1294 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 895 3695 895
Size: 2800 1378
SexAI A'CCwGG_T
Cuts at: 926 926
Size: 4178
SfcI C'TryA_G
Cuts at: 33 579 1221 1906 2899 3090 33
Size: 546 642 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 546 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 475 475
Size: 4178
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 808 1333 2248 2740 3002 808
Size: 525 915 492 262 1984
Fragments arranged by size:
1984 915 525 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StuI AGG'CCT
Cuts at: 257 257
Size: 4178
StyI C'CwwG_G
Cuts at: 107 1312 1666 107
Size: 1205 354 2619
Fragments arranged by size:
2619 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 861 927 1761 1894 2353 2562 861
Size: 66 834 133 459 209 2477
Fragments arranged by size:
2477 834 459 209 133 66
TatI w'GTAC_w
Cuts at: 209 893 3693 209
Size: 684 2800 694
Fragments arranged by size:
2800 694 684
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 3695 3727 844
Size: 32 2819 32 1295
Fragments arranged by size:
2819 1295 32 32
Tth111I GACn'n_nGTC
Cuts at: 928 1975 928
Size: 1047 3131
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 1027 1673 255
Size: 772 646 2760
Fragments arranged by size:
2760 772 646
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmtI BpmI Bpu10I BsiWI
BsmI BsrGI BstEII BstZ17I Bsu36I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI