Restriction map for
pF1KB7130
(Circular) (Six-base) MAPSORT of: pF1KB7130.seq Check: 1797 from: 1 to: 4061
pF1KB7130 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 878 1027 1886 2318 3065 3333 308
Size: 570 149 859 432 747 268 1036
Fragments arranged by size:
1036 859 747 570 432 268 149
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 248 280 248
Size: 32 4029
AlwNI CAG_nnn'CTG
Cuts at: 168 794 1366 2933 168
Size: 626 572 1567 1296
Fragments arranged by size:
1567 1296 626 572
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 1075 2105 1075
Size: 1030 3031
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BfrBI ATG'CAT
Cuts at: 576 576
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 1686 1779 2835 1686
Size: 93 1056 2912
Fragments arranged by size:
2912 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 917 1250 1771 2449 2723 2870 3281 917
Size: 333 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 333 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 248 278 248
Size: 30 4031
BseYI C'CCAG_C
Cuts at: 398 1486 2244 2821 3317 398
Size: 1088 758 577 496 1142
Fragments arranged by size:
1142 1088 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 575 381
Size: 194 3867
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2835 1075
Size: 778 190 792 2301
Fragments arranged by size:
2301 792 778 190
BsmI GAATG_Cn'
Cuts at: 576 576
Size: 4061
Bsp1286I G_dGCh'C
Cuts at: 1075 1686 1779 1853 2043 2105 2835 1075
Size: 611 93 74 190 62 730 2301
Fragments arranged by size:
2301 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 506 584 3237 506
Size: 78 2653 1330
Fragments arranged by size:
2653 1330 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 651 2353 2407 3891 4056 50
Size: 601 1702 54 1484 165 55
Fragments arranged by size:
1702 1484 601 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 355 2332 2690 355
Size: 1977 358 1726
Fragments arranged by size:
1977 1726 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 4061
BstYI r'GATC_y
Cuts at: 1 229 1086 1553 1576 1911 2157 3169 3255
Size: 228 857 467 23 335 246 1012 86
Cuts at: 3255 3267 3613 1
Size: 12 346 449
Fragments arranged by size:
1012 857 467 449 346 335 246 228
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 979 979
Size: 4061
BtgI C'CryG_G
Cuts at: 107 722 107
Size: 615 3446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 331 1304 331
Size: 973 3088
DraI TTT'AAA
Cuts at: 1035 1060 1035
Size: 25 4036
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 926 1646 1820 2211 2238 3583 926
Size: 720 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 494 749 2084 2294 494
Size: 255 1335 210 2261
Fragments arranged by size:
2261 1335 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 878 1027 1886 2318 3065 3333 308
Size: 570 149 859 432 747 268 1036
Fragments arranged by size:
1036 859 747 570 432 268 149
EcoO109I rG'GnC_Cy
Cuts at: 791 1200 791
Size: 409 3652
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 1032 2080 669
Size: 363 1048 2650
Fragments arranged by size:
2650 1048 363
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 944 1495 1743 2765 3744 944
Size: 551 248 1022 979 1261
Fragments arranged by size:
1261 1022 979 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 987 1019 1903 1933 1935 1965 987
Size: 32 884 30 2 30 3083
Fragments arranged by size:
3083 884 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 928 1822 928
Size: 894 3167
MslI CAynn'nnrTG
Cuts at: 124 721 904 2050 2177 124
Size: 597 183 1146 127 2008
Fragments arranged by size:
2008 1146 597 183 127
MspA1I CmG'CkG
Cuts at: 538 1172 1486 1846 2859 3104 538
Size: 634 314 360 1013 245 1495
Fragments arranged by size:
1495 1013 634 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 722 107
Size: 615 3446
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NsiI A_TGCA'T
Cuts at: 578 578
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PflMI CCAn_nnn'nTGG
Cuts at: 420 840 420
Size: 420 3641
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 248 280 3200 3232 248
Size: 32 2920 32 1077
Fragments arranged by size:
2920 1077 32 32
PpuMI rG'GwC_Cy
Cuts at: 791 791
Size: 4061
PstI C_TGCA'G
Cuts at: 173 799 1108 1793 173
Size: 626 309 685 2441
Fragments arranged by size:
2441 685 626 309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 538 1486 1846 538
Size: 948 360 2753
Fragments arranged by size:
2753 948 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 169 666 795 1104 1789 2782 2973 33
Size: 136 497 129 309 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 309 191 136 129
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2623 2885 1216
Size: 915 492 262 2392
Fragments arranged by size:
2392 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 722 1195 1549 107
Size: 615 473 354 2619
Fragments arranged by size:
2619 615 473 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 412 1644 1777 2236 2445 412
Size: 1232 133 459 209 2028
Fragments arranged by size:
2028 1232 459 209 133
TatI w'GTAC_w
Cuts at: 954 3576 954
Size: 2622 1439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1556 1556
Size: 4061
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
XmnI GAAnn'nnTTC
Cuts at: 297 297
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI