Restriction map for
pF1KB7129
(Circular) (Six-base) MAPSORT of: pF1KB7129.seq Check: 3160 from: 1 to: 4061
pF1KB7129 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 875 968 1099 875
Size: 93 131 3837
Fragments arranged by size:
3837 131 93
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 3065 3333 1886
Size: 432 747 268 2614
Fragments arranged by size:
2614 747 432 268
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 345 1366 2933 345
Size: 1021 1567 1473
Fragments arranged by size:
1567 1473 1021
ApaI G_GGCC'C
Cuts at: 364 364
Size: 4061
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 1065 3414 3670 1065
Size: 2349 256 1456
Fragments arranged by size:
2349 1456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 364 1075 2105 364
Size: 711 1030 2320
Fragments arranged by size:
2320 1030 711
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 186 376 186
Size: 190 3871
BbvCI CC'TCA_GC
Cuts at: 778 778
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 364 946 1686 1779 2835 364
Size: 582 740 93 1056 1590
Fragments arranged by size:
1590 1056 740 582 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1017 1065 865
Size: 152 48 3861
Fragments arranged by size:
3861 152 48
Bpu10I CC'TnA_GC
Cuts at: 778 778
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 931 3765 931
Size: 2834 1227
BsaAI yAC'GTr
Cuts at: 471 1414 471
Size: 943 3118
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 106
Size: 30 4031
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 816 816
Size: 4061
BseYI C'CCAG_C
Cuts at: 162 1486 2244 2821 3317 162
Size: 1324 758 577 496 906
Fragments arranged by size:
1324 906 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1002 1075 1853 2043 2835 1002
Size: 73 778 190 792 2228
Fragments arranged by size:
2228 792 778 190 73
Bsp1286I G_dGCh'C
Cuts at: 364 946 1002 1075 1686 1779 1853 2043 2105
Size: 582 56 73 611 93 74 190 62
Cuts at: 2105 2835 364
Size: 730 1590
Fragments arranged by size:
1590 730 611 582 190 93 74 73
62 56
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 1111 1325 1627 2008 829
Size: 282 214 302 381 2882
Fragments arranged by size:
2882 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 866 1973 866
Size: 1107 2954
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstEII G'GTnAC_C
Cuts at: 843 843
Size: 4061
BstYI r'GATC_y
Cuts at: 1 1086 1553 1576 1911 2157 3169 3255 3267
Size: 1085 467 23 335 246 1012 86 12
Cuts at: 3267 3613 1
Size: 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 969 969
Size: 4061
Bsu36I CC'TnA_GG
Cuts at: 976 976
Size: 4061
BtgI C'CryG_G
Cuts at: 107 128 107
Size: 21 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 761 779 944 1304 761
Size: 18 165 360 3518
Fragments arranged by size:
3518 360 165 18
ClaI AT'CG_AT
Cuts at: 568 568
Size: 4061
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DraIII CAC_nnn'GTG
Cuts at: 434 434
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 1646 1820 2211 2238 3583 1646
Size: 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 409 856 934 2084 2294 409
Size: 447 78 1150 210 2176
Fragments arranged by size:
2176 1150 447 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1017 1065 1886 2318 3065 3333 865
Size: 152 48 821 432 747 268 1593
Fragments arranged by size:
1593 821 747 432 268 152 48
EcoO109I rG'GnC_Cy
Cuts at: 360 642 818 1200 360
Size: 282 176 382 3221
Fragments arranged by size:
3221 382 282 176
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 380 412 631 663 1903 1933 1935 1965 380
Size: 32 219 32 1240 30 2 30 2476
Fragments arranged by size:
2476 1240 219 32 32 30 30 2
HincII GTy'rAC
Cuts at: 114 351 1100 114
Size: 237 749 3075
Fragments arranged by size:
3075 749 237
HpaI GTT'AAC
Cuts at: 114 114
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 718 2050 2177 718
Size: 1332 127 2602
Fragments arranged by size:
2602 1332 127
MspA1I CmG'CkG
Cuts at: 531 1172 1486 1846 2859 3104 531
Size: 641 314 360 1013 245 1488
Fragments arranged by size:
1488 1013 641 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 128 107
Size: 21 4040
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PasI CC'CwG_GG
Cuts at: 420 420
Size: 4061
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PflMI CCAn_nnn'nTGG
Cuts at: 345 800 345
Size: 455 3606
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PpuMI rG'GwC_Cy
Cuts at: 642 818 642
Size: 176 3885
PspOMI G'GGCC_C
Cuts at: 360 360
Size: 4061
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 531 1486 1846 531
Size: 955 360 2746
Fragments arranged by size:
2746 955 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 409 2084 2294 409
Size: 1675 210 2176
Fragments arranged by size:
2176 1675 210
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 855 1104 1789 2782 2973 33
Size: 822 249 685 993 191 1121
Fragments arranged by size:
1121 993 822 685 249 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2623 2885 1216
Size: 915 492 262 2392
Fragments arranged by size:
2392 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StuI AGG'CCT
Cuts at: 811 811
Size: 4061
StyI C'CwwG_G
Cuts at: 107 128 903 1195 1549 107
Size: 21 775 292 354 2619
Fragments arranged by size:
2619 775 354 292 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 176 479 1644 1777 2236 2445 176
Size: 303 1165 133 459 209 1792
Fragments arranged by size:
1792 1165 459 303 209 133
TatI w'GTAC_w
Cuts at: 249 278 3576 249
Size: 29 3298 734
Fragments arranged by size:
3298 734 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 221 253 3578 3610 221
Size: 32 3325 32 672
Fragments arranged by size:
3325 672 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 1556 381
Size: 1175 2886
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI CspCI
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI