Restriction map for
pF1KB7125
(Circular) (Six-base) MAPSORT of: pF1KB7125.seq Check: 7714 from: 1 to: 4049
pF1KB7125 4049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 131 1087 131
Size: 956 3093
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1874 2306 3053 3321 1874
Size: 432 747 268 2602
Fragments arranged by size:
2602 747 432 268
AflIII A'CryG_T
Cuts at: 1401 2505 3482 1401
Size: 1104 977 1968
Fragments arranged by size:
1968 1104 977
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 851 851
Size: 4049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 361 973 361
Size: 612 3437
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 237 1053 3402 3658 237
Size: 816 2349 256 628
Fragments arranged by size:
2349 816 628 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BamHI G'GATC_C
Cuts at: 161 1074 161
Size: 913 3136
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 287 1063 2093 287
Size: 776 1030 2243
Fragments arranged by size:
2243 1030 776
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BbsI GAAGACnn'nnnn_
Cuts at: 849 849
Size: 4049
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 1569 1569
Size: 4049
BglII A'GATC_T
Cuts at: 1564 3601 1564
Size: 2037 2012
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BmtI G_CTAG'C
Cuts at: 338 338
Size: 4049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 847
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2104 2596 2894 1225
Size: 879 492 298 2380
Fragments arranged by size:
2380 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 917 3753 917
Size: 2836 1213
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 781 1563 3600 6
Size: 775 782 2037 455
Fragments arranged by size:
2037 782 775 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 802
Size: 30 4019
BseYI C'CCAG_C
Cuts at: 1474 2232 2809 3305 1474
Size: 758 577 496 2218
Fragments arranged by size:
2218 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 4049
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmI GAATG_Cn'
Cuts at: 826 1016 826
Size: 190 3859
Bsp1286I G_dGCh'C
Cuts at: 287 1063 1674 1767 1841 2031 2093 2823 287
Size: 776 611 93 74 190 62 730 1513
Fragments arranged by size:
1513 776 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 3225 3225
Size: 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1099 1313 1615 1996 844
Size: 255 214 302 381 2897
Fragments arranged by size:
2897 381 302 255 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 221 323 430 839 1961 221
Size: 102 107 409 1122 2309
Fragments arranged by size:
2309 1122 409 107 102
BsrFI r'CCGG_y
Cuts at: 608 2047 2228 2437 608
Size: 1439 181 209 2220
Fragments arranged by size:
2220 1439 209 181
BsrGI T'GTAC_A
Cuts at: 290 290
Size: 4049
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 1426
Size: 4049
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 471 471
Size: 4049
BstYI r'GATC_y
Cuts at: 1 161 1074 1541 1564 1899 2145 3157 3243
Size: 160 913 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 913 467 449 346 335 246 160
86 23 12
BtgI C'CryG_G
Cuts at: 464 464
Size: 4049
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 190 1292 190
Size: 1102 2947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 306 341 306
Size: 35 4014
DraI TTT'AAA
Cuts at: 822 1048 822
Size: 226 3823
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 461 485 662 1634 1808 2199 2226 3571 461
Size: 24 177 972 174 391 27 1345 939
Fragments arranged by size:
1345 972 939 391 177 174 27 24
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 1874 2306 3053 3321 847
Size: 1027 432 747 268 1575
Fragments arranged by size:
1575 1027 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1188 1188
Size: 4049
EcoRI G'AATT_C
Cuts at: 1053 1053
Size: 4049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 4049
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 731 763 1891 1921 1923 1953 731
Size: 32 1128 30 2 30 2827
Fragments arranged by size:
2827 1128 32 30 30 2
HincII GTy'rAC
Cuts at: 149 699 1088 149
Size: 550 389 3110
Fragments arranged by size:
3110 550 389
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 143 2720 2904 143
Size: 2577 184 1288
Fragments arranged by size:
2577 1288 184
MscI TGG'CCA
Cuts at: 463 487 664 1810 463
Size: 24 177 1146 2702
Fragments arranged by size:
2702 1146 177 24
MslI CAynn'nnrTG
Cuts at: 226 457 851 1021 2038 2165 226
Size: 231 394 170 1017 127 2110
Fragments arranged by size:
2110 1017 394 231 170 127
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NcoI C'CATG_G
Cuts at: 464 464
Size: 4049
NdeI CA'TA_TG
Cuts at: 659 659
Size: 4049
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NheI G'CTAG_C
Cuts at: 334 334
Size: 4049
NspI r_CATG'y
Cuts at: 138 309 511 1102 1602 2509 138
Size: 171 202 591 500 907 1678
Fragments arranged by size:
1678 907 591 500 202 171
PciI A'CATG_T
Cuts at: 2505 2505
Size: 4049
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3188 3220 3188
Size: 32 4017
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 596 3566 596
Size: 2970 1079
SfcI C'TryA_G
Cuts at: 33 731 1092 1777 2770 2961 33
Size: 698 361 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 361 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 1204 2119 2611 2873 1204
Size: 915 492 262 2380
Fragments arranged by size:
2380 915 492 262
SpeI A'CTAG_T
Cuts at: 67 153 67
Size: 86 3963
SphI G_CATG'C
Cuts at: 309 1102 1602 309
Size: 793 500 2756
Fragments arranged by size:
2756 793 500
SspI AAT'ATT
Cuts at: 365 365
Size: 4049
StuI AGG'CCT
Cuts at: 170 170
Size: 4049
StyI C'CwwG_G
Cuts at: 464 1183 1537 464
Size: 719 354 2976
Fragments arranged by size:
2976 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 538 1632 1765 2224 2433 538
Size: 1094 133 459 209 2154
Fragments arranged by size:
2154 1094 459 209 133
TatI w'GTAC_w
Cuts at: 290 594 3564 290
Size: 304 2970 775
Fragments arranged by size:
2970 775 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I
BseRI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI