Restriction map for
pF1KB7123
(Circular) (Six-base) MAPSORT of: pF1KB7123.seq Check: 4429 from: 1 to: 4046
pF1KB7123 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 922 1084 922
Size: 162 3884
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 702 1871 2303 3050 3318 317
Size: 385 1169 432 747 268 1045
Fragments arranged by size:
1169 1045 747 432 385 268
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AleI CACnn'nnGTG
Cuts at: 607 607
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 610 2816 610
Size: 2206 1840
ApoI r'AATT_y
Cuts at: 702 1050 3399 3655 702
Size: 348 2349 256 1093
Fragments arranged by size:
2349 1093 348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 1066 1066
Size: 4046
BamHI G'GATC_C
Cuts at: 222 1071 222
Size: 849 3197
BanI G'GyrC_C
Cuts at: 514 664 1062 1476 1724 1759 514
Size: 150 398 414 248 35 2801
Fragments arranged by size:
2801 414 398 248 150 35
BanII G_rGCy'C
Cuts at: 508 686 1060 2090 508
Size: 178 374 1030 2464
Fragments arranged by size:
2464 1030 374 178
BbeI G_GCGC'C
Cuts at: 518 1480 1728 518
Size: 962 248 2836
Fragments arranged by size:
2836 962 248
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 452 1566 452
Size: 1114 2932
BglI GCCn_nnn'nGGC
Cuts at: 512 622 512
Size: 110 3936
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 614 654 1671 1764 2820 614
Size: 40 1017 93 1056 1840
Fragments arranged by size:
1840 1056 1017 93 40
BmrI ACTGGGnnnn_n'
Cuts at: 664 1258 1339 1674 3818 664
Size: 594 81 335 2144 892
Fragments arranged by size:
2144 892 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 437 3750 437
Size: 3313 733
BsaAI yAC'GTr
Cuts at: 1399 1399
Size: 4046
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 515 1477 1725 2427 3923 515
Size: 962 248 702 1496 638
Fragments arranged by size:
1496 962 702 638 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 185 215 434 464 745 775 904 934 185
Size: 30 219 30 281 30 129 30 3297
Fragments arranged by size:
3297 281 219 129 30 30 30 30
BseYI C'CCAG_C
Cuts at: 406 507 627 928 1471 2229 2806 3302 406
Size: 101 120 301 543 758 577 496 1150
Fragments arranged by size:
1150 758 577 543 496 301 120 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 200 404 163
Size: 37 204 3805
Fragments arranged by size:
3805 204 37
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 614 1060 1838 2028 2820 614
Size: 446 778 190 792 1840
Fragments arranged by size:
1840 792 778 446 190
Bsp1286I G_dGCh'C
Cuts at: 508 614 654 686 1060 1671 1764 1838 2028
Size: 106 40 32 374 611 93 74 190
Cuts at: 2028 2090 2820 508
Size: 62 730 1734
Fragments arranged by size:
1734 730 611 374 190 106 93 74
62 40 32
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 103 1096 1310 1612 1993 103
Size: 993 214 302 381 2156
Fragments arranged by size:
2156 993 381 302 214
BsrBI CCG'CTC
Cuts at: 50 276 1037 2338 2392 3876 4041 50
Size: 226 761 1301 54 1484 165 55
Fragments arranged by size:
1484 1301 761 226 165 55 54
BsrDI GCAATG_nn'
Cuts at: 339 1958 339
Size: 1619 2427
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 618 832 1423 618
Size: 214 591 3241
Fragments arranged by size:
3241 591 214
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 438 579 438
Size: 141 3905
BstYI r'GATC_y
Cuts at: 1 222 1071 1538 1561 1896 2142 3154 3240
Size: 221 849 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 849 467 449 346 335 246 221
86 23 12
BstZ17I GTA'TAC
Cuts at: 923 923
Size: 4046
Bsu36I CC'TnA_GG
Cuts at: 257 257
Size: 4046
BtgI C'CryG_G
Cuts at: 107 227 470 785 107
Size: 120 243 315 3368
Fragments arranged by size:
3368 315 243 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 602 740 1289 602
Size: 138 549 3359
Fragments arranged by size:
3359 549 138
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 1752 2610 1752
Size: 858 3188
EaeI y'GGCC_r
Cuts at: 569 1631 1805 2196 2223 3568 569
Size: 1062 174 391 27 1345 1047
Fragments arranged by size:
1345 1062 1047 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 220 304 2069 2279 220
Size: 84 1765 210 1987
Fragments arranged by size:
1987 1765 210 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 141 516 2254 2564 2710 3856 141
Size: 375 1738 310 146 1146 331
Fragments arranged by size:
1738 1146 375 331 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 702 1871 2303 3050 3318 317
Size: 385 1169 432 747 268 1045
Fragments arranged by size:
1169 1045 747 432 385 268
EcoNI CCTnn'n_nnAGG
Cuts at: 570 570
Size: 4046
EcoO109I rG'GnC_Cy
Cuts at: 115 644 694 1185 115
Size: 529 50 491 2976
Fragments arranged by size:
2976 529 491 50
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 582 2065 582
Size: 1483 2563
FspI TGC'GCA
Cuts at: 889 1827 889
Size: 938 3108
HaeII r_GCGC'y
Cuts at: 518 1480 1728 2750 3729 518
Size: 962 248 1022 979 835
Fragments arranged by size:
1022 979 962 835 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 185 217 1011 1043 1888 1918 1920 1950 185
Size: 32 794 32 845 30 2 30 2281
Fragments arranged by size:
2281 845 794 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1085 1085
Size: 4046
HindIII A'AGCT_T
Cuts at: 862 862
Size: 4046
KasI G'GCGC_C
Cuts at: 514 1476 1724 514
Size: 962 248 2836
Fragments arranged by size:
2836 962 248
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 571 1807 571
Size: 1236 2810
MslI CAynn'nnrTG
Cuts at: 607 2035 2162 607
Size: 1428 127 2491
Fragments arranged by size:
2491 1428 127
MspA1I CmG'CkG
Cuts at: 288 932 1157 1471 1831 2844 3089 288
Size: 644 225 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 644 360 314 245 225
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 515 1477 1725 515
Size: 962 248 2836
Fragments arranged by size:
2836 962 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4046
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PasI CC'CwG_GG
Cuts at: 678 678
Size: 4046
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PflMI CCAn_nnn'nTGG
Cuts at: 471 471
Size: 4046
PfoI T'CCnGG_A
Cuts at: 218 218
Size: 4046
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PpuMI rG'GwC_Cy
Cuts at: 115 644 115
Size: 529 3517
PstI C_TGCA'G
Cuts at: 293 828 1007 1093 1778 293
Size: 535 179 86 685 2561
Fragments arranged by size:
2561 685 535 179 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 288 1471 1831 288
Size: 1183 360 2503
Fragments arranged by size:
2503 1183 360
RsrII CG'GwC_CG
Cuts at: 128 2241 128
Size: 2113 1933
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 250 3563 250
Size: 3313 733
SfcI C'TryA_G
Cuts at: 33 289 313 824 1003 1089 1774 2767 2958
Size: 256 24 511 179 86 685 993 191
Cuts at: 2958 33
Size: 1121
Fragments arranged by size:
1121 993 685 511 256 191 179 86
24
SfoI GGC'GCC
Cuts at: 516 1478 1726 516
Size: 962 248 2836
Fragments arranged by size:
2836 962 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StuI AGG'CCT
Cuts at: 161 185 161
Size: 24 4022
StyI C'CwwG_G
Cuts at: 107 566 1180 1534 107
Size: 459 614 354 2619
Fragments arranged by size:
2619 614 459 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 652 942 1629 1762 2221 2430 652
Size: 290 687 133 459 209 2268
Fragments arranged by size:
2268 687 459 290 209 133
TatI w'GTAC_w
Cuts at: 248 671 3561 248
Size: 423 2890 733
Fragments arranged by size:
2890 733 423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 434 450 466 482 3563 3595 434
Size: 16 16 16 3081 32 885
Fragments arranged by size:
3081 885 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 540 1843 540
Size: 1303 2743
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 675 1541 675
Size: 866 3180
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI