Restriction map for
pF1KB7122
(Circular) (Six-base) MAPSORT of: pF1KB7122.seq Check: 1300 from: 1 to: 4040
pF1KB7122 4040 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 350 1078 350
Size: 728 3312
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1865 2297 3044 3312 796
Size: 1069 432 747 268 1524
Fragments arranged by size:
1524 1069 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AleI CACnn'nnGTG
Cuts at: 519 519
Size: 4040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 301
Size: 32 4008
AlwNI CAG_nnn'CTG
Cuts at: 264 1345 2912 264
Size: 1081 1567 1392
Fragments arranged by size:
1567 1392 1081
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 133 1044 3393 3649 133
Size: 911 2349 256 524
Fragments arranged by size:
2349 911 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 3744 154
Size: 3590 450
BsaAI yAC'GTr
Cuts at: 471 1393 471
Size: 922 3118
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 373 343
Size: 30 4010
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 433
Size: 4040
BseYI C'CCAG_C
Cuts at: 398 1027 1465 2223 2800 3296 398
Size: 629 438 758 577 496 1142
Fragments arranged by size:
1142 758 629 577 496 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 651 475
Size: 176 3864
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 370 1054 1832 2022 2814 370
Size: 684 778 190 792 1596
Fragments arranged by size:
1596 792 778 684 190
BsmI GAATG_Cn'
Cuts at: 978 978
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 370 1054 1665 1758 1832 2022 2084 2814 370
Size: 684 611 93 74 190 62 730 1596
Fragments arranged by size:
1596 730 684 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 506 3216 506
Size: 2710 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 826 1090 1304 1606 1987 826
Size: 264 214 302 381 2879
Fragments arranged by size:
2879 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 575 917 1952 575
Size: 342 1035 2663
Fragments arranged by size:
2663 1035 342
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BsrGI T'GTAC_A
Cuts at: 872 872
Size: 4040
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 269 269
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 219 219
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 514 107
Size: 407 3633
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 473 725 1625 1799 2190 2217 3562 473
Size: 252 900 174 391 27 1345 951
Fragments arranged by size:
1345 951 900 391 252 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 589 2063 2273 589
Size: 1474 210 2356
Fragments arranged by size:
2356 1474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 796 1865 2297 3044 3312 184
Size: 612 1069 432 747 268 912
Fragments arranged by size:
1069 912 747 612 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 133 1044 133
Size: 911 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 1882 1912 1914 1944 343
Size: 32 1507 30 2 30 2439
Fragments arranged by size:
2439 1507 32 30 30 2
HincII GTy'rAC
Cuts at: 656 1079 656
Size: 423 3617
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 475 727 1801 475
Size: 252 1074 2714
Fragments arranged by size:
2714 1074 252
MslI CAynn'nnrTG
Cuts at: 451 519 940 2029 2156 451
Size: 68 421 1089 127 2335
Fragments arranged by size:
2335 1089 421 127 68
MspA1I CmG'CkG
Cuts at: 402 1151 1465 1825 2838 3083 402
Size: 749 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 749 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 684 684
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PstI C_TGCA'G
Cuts at: 823 1087 1772 823
Size: 264 685 3091
Fragments arranged by size:
3091 685 264
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 402 1465 1825 402
Size: 1063 360 2617
Fragments arranged by size:
2617 1063 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 819 1083 1768 2761 2952 33
Size: 786 264 685 993 191 1121
Fragments arranged by size:
1121 993 786 685 264 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
SspI AAT'ATT
Cuts at: 179 179
Size: 4040
StuI AGG'CCT
Cuts at: 882 882
Size: 4040
StyI C'CwwG_G
Cuts at: 107 338 1174 1528 107
Size: 231 836 354 2619
Fragments arranged by size:
2619 836 354 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 412 537 691 954 1623 1756 2215 2424 412
Size: 125 154 263 669 133 459 209 2028
Fragments arranged by size:
2028 669 459 263 209 154 133 125
TatI w'GTAC_w
Cuts at: 872 3555 872
Size: 2683 1357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 3557 3589 705
Size: 32 2820 32 1156
Fragments arranged by size:
2820 1156 32 32
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1535 1535
Size: 4040
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 137 137
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI