Restriction map for
pF1KB7121
(Circular) (Six-base) MAPSORT of: pF1KB7121.seq Check: 3754 from: 1 to: 4028
pF1KB7121 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 599 930 1853 2285 3032 3300 319
Size: 280 331 923 432 747 268 1047
Fragments arranged by size:
1047 923 747 432 331 280 268
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 167 1333 2900 167
Size: 1166 1567 1295
Fragments arranged by size:
1567 1295 1166
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 1032 3381 3637 1032
Size: 2349 256 1423
Fragments arranged by size:
2349 1423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 740 1048 740
Size: 308 3720
BamHI G'GATC_C
Cuts at: 671 1053 671
Size: 382 3646
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 116 487 786 815 884 1042 2072 116
Size: 371 299 29 69 158 1030 2072
Fragments arranged by size:
2072 1030 371 299 158 69 29
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 605 605
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 683 1548 683
Size: 865 3163
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 1653 1746 2802 1653
Size: 93 1056 2879
Fragments arranged by size:
2879 1056 93
BmgBI CAC'GTC
Cuts at: 611 611
Size: 4028
BmrI ACTGGGnnnn_n'
Cuts at: 677 1240 1321 1656 3800 677
Size: 563 81 335 2144 905
Fragments arranged by size:
2144 905 563 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 241 2395 241
Size: 2154 1874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 281 778 189
Size: 92 497 3439
Fragments arranged by size:
3439 497 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 836 1459 1707 2409 3905 836
Size: 623 248 702 1496 959
Fragments arranged by size:
1496 959 702 623 248
BsaWI w'CCGG_w
Cuts at: 479 1217 1738 2416 2690 2837 3248 479
Size: 738 521 678 274 147 411 1259
Fragments arranged by size:
1259 738 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 300 303 598 811 300
Size: 3 295 213 3517
Fragments arranged by size:
3517 295 213 3
BseYI C'CCAG_C
Cuts at: 527 777 828 1453 2211 2788 3284 527
Size: 250 51 625 758 577 496 1271
Fragments arranged by size:
1271 758 625 577 496 250 51
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 116 487 884 1042 1820 2010 2802 116
Size: 371 397 158 778 190 792 1342
Fragments arranged by size:
1342 792 778 397 371 190 158
Bsp1286I G_dGCh'C
Cuts at: 116 487 786 815 884 1042 1653 1746 1820
Size: 371 299 29 69 158 611 93 74
Cuts at: 1820 2010 2072 2802 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 730 611 371 299 190 158 93
74 69 62 29
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BsrGI T'GTAC_A
Cuts at: 1014 1014
Size: 4028
BssSI C'ACGA_G
Cuts at: 679 2299 2657 679
Size: 1620 358 2050
Fragments arranged by size:
2050 1620 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstEII G'GTnAC_C
Cuts at: 190 190
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 545 732 545
Size: 187 3841
BstYI r'GATC_y
Cuts at: 1 671 1053 1520 1543 1878 2124 3136 3222
Size: 670 382 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 670 467 449 382 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 703 107
Size: 596 3432
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 1613 1787 2178 2205 3550 1613
Size: 174 391 27 1345 2091
Fragments arranged by size:
2091 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 127 338 575 912 2051 2261 127
Size: 211 237 337 1139 210 1894
Fragments arranged by size:
1894 1139 337 237 211 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 114 485 882 1040 114
Size: 371 397 158 3102
Fragments arranged by size:
3102 397 371 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 281 319 599 778 930 1853 2285 3032
Size: 92 38 280 179 152 923 432 747
Cuts at: 3032 3300 189
Size: 268 917
Fragments arranged by size:
923 917 747 432 280 268 179 152
92 38
EcoNI CCTnn'n_nnAGG
Cuts at: 760 760
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
EcoRV GAT'ATC
Cuts at: 575 575
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1024 2047 1024
Size: 1023 3005
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 650 756 1462 1710 2732 3711 650
Size: 106 706 248 1022 979 967
Fragments arranged by size:
1022 979 967 706 248 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1870 1900 1902 1932 1870
Size: 30 2 30 3966
Fragments arranged by size:
3966 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 2017 2144 2017
Size: 127 3901
MspA1I CmG'CkG
Cuts at: 391 474 797 1139 1453 1813 2826 3071 391
Size: 83 323 342 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 360 342 323 314 245 83
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PasI CC'CwG_GG
Cuts at: 514 514
Size: 4028
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 167 167
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 469 501 3167 3199 469
Size: 32 2666 32 1298
Fragments arranged by size:
2666 1298 32 32
PstI C_TGCA'G
Cuts at: 418 1075 1760 418
Size: 657 685 2686
Fragments arranged by size:
2686 685 657
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 116 487 884 1042 116
Size: 371 397 158 3102
Fragments arranged by size:
3102 397 371 158
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 127 2051 2261 127
Size: 1924 210 1894
Fragments arranged by size:
1924 1894 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 330 3545 330
Size: 3215 813
SfcI C'TryA_G
Cuts at: 33 414 626 1071 1756 2749 2940 33
Size: 381 212 445 685 993 191 1121
Fragments arranged by size:
1121 993 685 445 381 212 191
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 742 1050 742
Size: 308 3720
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 989 1162 1516 107
Size: 882 173 354 2619
Fragments arranged by size:
2619 882 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 328 1014 3543 328
Size: 686 2529 813
Fragments arranged by size:
2529 813 686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 1523 657
Size: 866 3162
XmaI C'CCGG_G
Cuts at: 740 1048 740
Size: 308 3720
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI