Restriction map for pF1KB7120
 (Circular) (Six-base) MAPSORT of: pF1KB7120.seq  Check: 1193  from: 1  to: 3995



pF1KB7120  3995 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    366     382    1033     366 
   Size:         16     651    3328

  Fragments arranged by size: 

                3328     651      16
 
Acc65I G'GTAC_C

Cuts at:   1011    1011 
   Size:       3995
 
AclI AA'CG_TT

Cuts at:   3835    3835 
   Size:       3995
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    470     662    1820    2252    2999    3267     470 
   Size:        192    1158     432     747     268    1198

  Fragments arranged by size: 

                1198    1158     747     432     268     192
 
AflII C'TTAA_G

Cuts at:    370     370 
   Size:       3995
 
AflIII A'CryG_T

Cuts at:   1347    2451    3428    1347 
   Size:       1104     977    1914

  Fragments arranged by size: 

                1914    1104     977
 
AgeI A'CCGG_T

Cuts at:   2383    2383 
   Size:       3995
 
AlwNI CAG_nnn'CTG

Cuts at:   1300    2867    1300 
   Size:       1567    2428
 
ApaLI G'TGCA_C

Cuts at:   2765    2765 
   Size:       3995
 
ApoI r'AATT_y

Cuts at:    316     404     907     999    3348    3604     316 
   Size:         88     503      92    2349     256     707

  Fragments arranged by size: 

                2349     707     503     256      92      88
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3995
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3995
 
AvaI C'yCGr_G

Cuts at:   1015    1015 
   Size:       3995
 
BamHI G'GATC_C

Cuts at:   1020    1020 
   Size:       3995
 
BanI G'GyrC_C

Cuts at:   1011    1425    1673    1708    1011 
   Size:        414     248      35    3298

  Fragments arranged by size: 

                3298     414     248      35
 
BanII G_rGCy'C

Cuts at:   1009    2039    1009 
   Size:       1030    2965
 
BbeI G_GCGC'C

Cuts at:   1429    1677    1429 
   Size:        248    3747
 
BciVI GTATCCnnnnn_n'

Cuts at:    494    1893    2654    3995     494 
   Size:       1399     761    1341     494

  Fragments arranged by size: 

                1399    1341     761     494
 
BclI T'GATC_A

Cuts at:   1515    1515 
   Size:       3995
 
BglII A'GATC_T

Cuts at:   1510    3547    1510 
   Size:       2037    1958
 
BlpI GC'TnA_GC

Cuts at:   1107    1107 
   Size:       3995
 
Bme1580I G_kGCm'C

Cuts at:   1620    1713    2769    1620 
   Size:         93    1056    2846

  Fragments arranged by size: 

                2846    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1207    1288    1623    3767    1207 
   Size:         81     335    2144    1435

  Fragments arranged by size: 

                2144    1435     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2362    2362 
   Size:       3995
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1171    2050    2542    2840    1171 
   Size:        879     492     298    2326

  Fragments arranged by size: 

                2326     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3699    3699 
   Size:       3995
 
BsaAI yAC'GTr

Cuts at:   1348    1348 
   Size:       3995
 
BsaBI GATnn'nnATC

Cuts at:      6    1509    3546       6 
   Size:       1503    2037     455

  Fragments arranged by size: 

                2037    1503     455
 
BsaHI Gr'CG_yC

Cuts at:   1426    1674    2376    3872    1426 
   Size:        248     702    1496    1549

  Fragments arranged by size: 

                1549    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1184    1705    2383    2657    2804    3215    1184 
   Size:        521     678     274     147     411    1964

  Fragments arranged by size: 

                1964     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1420    2178    2755    3251    1420 
   Size:        758     577     496    2164

  Fragments arranged by size: 

                2164     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1537    1583    2791     104 
   Size:       1433      46    1208    1308

  Fragments arranged by size: 

                1433    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1009    1787    1977    2769    1009 
   Size:        778     190     792    2235

  Fragments arranged by size: 

                2235     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1009    1620    1713    1787    1977    2039    2769    1009 
   Size:        611      93      74     190      62     730    2235

  Fragments arranged by size: 

                2235     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3215    3215 
   Size:       3995
 
BspHI T'CATG_A

Cuts at:   3171    3171 
   Size:       3995
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1045    1259    1561    1942    1045 
   Size:        214     302     381    3098

  Fragments arranged by size: 

                3098     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2287    2341    3825    3990      50 
   Size:       2237      54    1484     165      55

  Fragments arranged by size: 

                2237    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    530    1907     530 
   Size:       1377    2618
 
BsrFI r'CCGG_y

Cuts at:   1993    2174    2383    1993 
   Size:        181     209    3605

  Fragments arranged by size: 

                3605     209     181
 
BssSI C'ACGA_G

Cuts at:   2266    2624    2266 
   Size:        358    3637
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1372    1372 
   Size:       3995
 
BstBI TT'CG_AA

Cuts at:   2356    2356 
   Size:       3995
 
BstYI r'GATC_y

Cuts at:      1     798    1020    1487    1510    1845    2091    3103    3189 
   Size:        797     222     467      23     335     246    1012      86

Cuts at:   3189    3201    3547       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     797     467     449     346     335     246     222
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1945    2126    2289    1945 
   Size:        181     163    3651

  Fragments arranged by size: 

                3651     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1238    1238 
   Size:       3995
 
DraI TTT'AAA

Cuts at:    994     994 
   Size:       3995
 
DrdI GACnn_nn'nnGTC

Cuts at:   1701    2559    1701 
   Size:        858    3137
 
EaeI y'GGCC_r

Cuts at:   1580    1754    2145    2172    3517    1580 
   Size:        174     391      27    1345    2058

  Fragments arranged by size: 

                2058    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1580    1580 
   Size:       3995
 
EarI CTCTTCn'nnn_

Cuts at:   2018    2228    2018 
   Size:        210    3785
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    520    2203    2513    2659    3805     520 
   Size:       1683     310     146    1146     710

  Fragments arranged by size: 

                1683    1146     710     310     146
 
EcoICRI GAG'CTC

Cuts at:   1007    1007 
   Size:       3995
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    470     662    1820    2252    2999    3267     470 
   Size:        192    1158     432     747     268    1198

  Fragments arranged by size: 

                1198    1158     747     432     268     192
 
EcoO109I rG'GnC_Cy

Cuts at:   1134    1134 
   Size:       3995
 
EcoRI G'AATT_C

Cuts at:    999     999 
   Size:       3995
 
EcoRV GAT'ATC

Cuts at:    815     815 
   Size:       3995
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    136     748     959    2014     136 
   Size:        612     211    1055    2117

  Fragments arranged by size: 

                2117    1055     612     211
 
FspI TGC'GCA

Cuts at:   1776    1776 
   Size:       3995
 
HaeII r_GCGC'y

Cuts at:   1429    1677    2699    3678    1429 
   Size:        248    1022     979    1746

  Fragments arranged by size: 

                1746    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    830     862    1837    1867    1869    1899     830 
   Size:         32     975      30       2      30    2926

  Fragments arranged by size: 

                2926     975      32      30      30       2
 
HincII GTy'rAC

Cuts at:    891    1034     891 
   Size:        143    3852
 
HindIII A'AGCT_T

Cuts at:    696     696 
   Size:       3995
 
HpaI GTT'AAC

Cuts at:    891     891 
   Size:       3995
 
KasI G'GCGC_C

Cuts at:   1425    1673    1425 
   Size:        248    3747
 
KpnI G_GTAC'C

Cuts at:   1015    1015 
   Size:       3995
 
MluI A'CGCG_T

Cuts at:   3428    3428 
   Size:       3995
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2666    2850    2666 
   Size:        184    3811
 
MscI TGG'CCA

Cuts at:   1756    1756 
   Size:       3995
 
MslI CAynn'nnrTG

Cuts at:    260    1984    2111     260 
   Size:       1724     127    2144

  Fragments arranged by size: 

                2144    1724     127
 
MspA1I CmG'CkG

Cuts at:   1106    1420    1780    2793    3038    1106 
   Size:        314     360    1013     245    2063

  Fragments arranged by size: 

                2063    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2176    2176 
   Size:       3995
 
NarI GG'CG_CC

Cuts at:   1426    1674    1426 
   Size:        248    3747
 
NgoMIV G'CCGG_C

Cuts at:   2174    2174 
   Size:       3995
 
NspI r_CATG'y

Cuts at:    520    1048    1548    2455     520 
   Size:        528     500     907    2060

  Fragments arranged by size: 

                2060     907     528     500
 
PciI A'CATG_T

Cuts at:   2451    2451 
   Size:       3995
 
PmeI GTTT'AAAC

Cuts at:    994     994 
   Size:       3995
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3134    3166    3134 
   Size:         32    3963
 
PstI C_TGCA'G

Cuts at:   1042    1727    1042 
   Size:        685    3310
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3995
 
PvuII CAG'CTG

Cuts at:   1420    1780    1420 
   Size:        360    3635
 
RsrII CG'GwC_CG

Cuts at:   2190    2190 
   Size:       3995
 
SacI G_AGCT'C

Cuts at:   1009    1009 
   Size:       3995
 
SalI G'TCGA_C

Cuts at:   1032    1032 
   Size:       3995
 
SapI GCTCTTCn'nnn_

Cuts at:   2018    2228    2018 
   Size:        210    3785
 
SbfI CC_TGCA'GG

Cuts at:   1042    1042 
   Size:       3995
 
ScaI AGT'ACT

Cuts at:    953    3512     953 
   Size:       2559    1436
 
SfcI C'TryA_G

Cuts at:     33     182     211     242     277     978    1038    1723    2716 
   Size:        149      29      31      35     701      60     685     993

Cuts at:   2716    2907      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     701     685     191     149      60      35
                  31      29
 
SfoI GGC'GCC

Cuts at:   1427    1675    1427 
   Size:        248    3747
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3995
 
SmaI CCC'GGG

Cuts at:   1017    1017 
   Size:       3995
 
SmlI C'TyrA_G

Cuts at:    370    1150    2065    2557    2819     370 
   Size:        780     915     492     262    1546

  Fragments arranged by size: 

                1546     915     780     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3995
 
SphI G_CATG'C

Cuts at:    520    1048    1548     520 
   Size:        528     500    2967

  Fragments arranged by size: 

                2967     528     500
 
SspI AAT'ATT

Cuts at:    855     855 
   Size:       3995
 
StyI C'CwwG_G

Cuts at:   1129    1483    1129 
   Size:        354    3641
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    131    1578    1711    2170    2379     131 
   Size:       1447     133     459     209    1747

  Fragments arranged by size: 

                1747    1447     459     209     133
 
TatI w'GTAC_w

Cuts at:    874     951    3510     874 
   Size:         77    2559    1359

  Fragments arranged by size: 

                2559    1359      77
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3512    3544    3512 
   Size:         32    3963
 
Tth111I GACn'n_nGTC

Cuts at:   1792    1792 
   Size:       3995
 
XbaI T'CTAG_A

Cuts at:    160    1026     160 
   Size:        866    3129
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1490    1490 
   Size:       3995
 
XmaI C'CCGG_G

Cuts at:   1015    1015 
   Size:       3995
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI   DraIII    EcoNI     FseI    FspAI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com