(Circular) (Six-base) MAPSORT of: pF1KB7120.seq Check: 1193 from: 1 to: 3995 pF1KB7120 3995 bp With 182 enzymes: SgfI * June 20, 2008 10:26 .. AccI GT'mk_AC Cuts at: 366 382 1033 366 Size: 16 651 3328 Fragments arranged by size: 3328 651 16 Acc65I G'GTAC_C Cuts at: 1011 1011 Size: 3995 AclI AA'CG_TT Cuts at: 3835 3835 Size: 3995 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 470 662 1820 2252 2999 3267 470 Size: 192 1158 432 747 268 1198 Fragments arranged by size: 1198 1158 747 432 268 192 AflII C'TTAA_G Cuts at: 370 370 Size: 3995 AflIII A'CryG_T Cuts at: 1347 2451 3428 1347 Size: 1104 977 1914 Fragments arranged by size: 1914 1104 977 AgeI A'CCGG_T Cuts at: 2383 2383 Size: 3995 AlwNI CAG_nnn'CTG Cuts at: 1300 2867 1300 Size: 1567 2428 ApaLI G'TGCA_C Cuts at: 2765 2765 Size: 3995 ApoI r'AATT_y Cuts at: 316 404 907 999 3348 3604 316 Size: 88 503 92 2349 256 707 Fragments arranged by size: 2349 707 503 256 92 88 AseI AT'TA_AT Cuts at: 20 20 Size: 3995 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3995 AvaI C'yCGr_G Cuts at: 1015 1015 Size: 3995 BamHI G'GATC_C Cuts at: 1020 1020 Size: 3995 BanI G'GyrC_C Cuts at: 1011 1425 1673 1708 1011 Size: 414 248 35 3298 Fragments arranged by size: 3298 414 248 35 BanII G_rGCy'C Cuts at: 1009 2039 1009 Size: 1030 2965 BbeI G_GCGC'C Cuts at: 1429 1677 1429 Size: 248 3747 BciVI GTATCCnnnnn_n' Cuts at: 494 1893 2654 3995 494 Size: 1399 761 1341 494 Fragments arranged by size: 1399 1341 761 494 BclI T'GATC_A Cuts at: 1515 1515 Size: 3995 BglII A'GATC_T Cuts at: 1510 3547 1510 Size: 2037 1958 BlpI GC'TnA_GC Cuts at: 1107 1107 Size: 3995 Bme1580I G_kGCm'C Cuts at: 1620 1713 2769 1620 Size: 93 1056 2846 Fragments arranged by size: 2846 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1207 1288 1623 3767 1207 Size: 81 335 2144 1435 Fragments arranged by size: 2144 1435 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2362 2362 Size: 3995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1171 2050 2542 2840 1171 Size: 879 492 298 2326 Fragments arranged by size: 2326 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3699 3699 Size: 3995 BsaAI yAC'GTr Cuts at: 1348 1348 Size: 3995 BsaBI GATnn'nnATC Cuts at: 6 1509 3546 6 Size: 1503 2037 455 Fragments arranged by size: 2037 1503 455 BsaHI Gr'CG_yC Cuts at: 1426 1674 2376 3872 1426 Size: 248 702 1496 1549 Fragments arranged by size: 1549 1496 702 248 BsaWI w'CCGG_w Cuts at: 1184 1705 2383 2657 2804 3215 1184 Size: 521 678 274 147 411 1964 Fragments arranged by size: 1964 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1420 2178 2755 3251 1420 Size: 758 577 496 2164 Fragments arranged by size: 2164 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1537 1583 2791 104 Size: 1433 46 1208 1308 Fragments arranged by size: 1433 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1009 1787 1977 2769 1009 Size: 778 190 792 2235 Fragments arranged by size: 2235 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1009 1620 1713 1787 1977 2039 2769 1009 Size: 611 93 74 190 62 730 2235 Fragments arranged by size: 2235 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3215 3215 Size: 3995 BspHI T'CATG_A Cuts at: 3171 3171 Size: 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1045 1259 1561 1942 1045 Size: 214 302 381 3098 Fragments arranged by size: 3098 381 302 214 BsrBI CCG'CTC Cuts at: 50 2287 2341 3825 3990 50 Size: 2237 54 1484 165 55 Fragments arranged by size: 2237 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 530 1907 530 Size: 1377 2618 BsrFI r'CCGG_y Cuts at: 1993 2174 2383 1993 Size: 181 209 3605 Fragments arranged by size: 3605 209 181 BssSI C'ACGA_G Cuts at: 2266 2624 2266 Size: 358 3637 BstAPI GCAn_nnn'nTGC Cuts at: 1372 1372 Size: 3995 BstBI TT'CG_AA Cuts at: 2356 2356 Size: 3995 BstYI r'GATC_y Cuts at: 1 798 1020 1487 1510 1845 2091 3103 3189 Size: 797 222 467 23 335 246 1012 86 Cuts at: 3189 3201 3547 1 Size: 12 346 449 Fragments arranged by size: 1012 797 467 449 346 335 246 222 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1945 2126 2289 1945 Size: 181 163 3651 Fragments arranged by size: 3651 181 163 BtsI GCAGTG_nn' Cuts at: 1238 1238 Size: 3995 DraI TTT'AAA Cuts at: 994 994 Size: 3995 DrdI GACnn_nn'nnGTC Cuts at: 1701 2559 1701 Size: 858 3137 EaeI y'GGCC_r Cuts at: 1580 1754 2145 2172 3517 1580 Size: 174 391 27 1345 2058 Fragments arranged by size: 2058 1345 391 174 27 EagI C'GGCC_G Cuts at: 1580 1580 Size: 3995 EarI CTCTTCn'nnn_ Cuts at: 2018 2228 2018 Size: 210 3785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 520 2203 2513 2659 3805 520 Size: 1683 310 146 1146 710 Fragments arranged by size: 1683 1146 710 310 146 EcoICRI GAG'CTC Cuts at: 1007 1007 Size: 3995 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 470 662 1820 2252 2999 3267 470 Size: 192 1158 432 747 268 1198 Fragments arranged by size: 1198 1158 747 432 268 192 EcoO109I rG'GnC_Cy Cuts at: 1134 1134 Size: 3995 EcoRI G'AATT_C Cuts at: 999 999 Size: 3995 EcoRV GAT'ATC Cuts at: 815 815 Size: 3995 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 136 748 959 2014 136 Size: 612 211 1055 2117 Fragments arranged by size: 2117 1055 612 211 FspI TGC'GCA Cuts at: 1776 1776 Size: 3995 HaeII r_GCGC'y Cuts at: 1429 1677 2699 3678 1429 Size: 248 1022 979 1746 Fragments arranged by size: 1746 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 830 862 1837 1867 1869 1899 830 Size: 32 975 30 2 30 2926 Fragments arranged by size: 2926 975 32 30 30 2 HincII GTy'rAC Cuts at: 891 1034 891 Size: 143 3852 HindIII A'AGCT_T Cuts at: 696 696 Size: 3995 HpaI GTT'AAC Cuts at: 891 891 Size: 3995 KasI G'GCGC_C Cuts at: 1425 1673 1425 Size: 248 3747 KpnI G_GTAC'C Cuts at: 1015 1015 Size: 3995 MluI A'CGCG_T Cuts at: 3428 3428 Size: 3995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2666 2850 2666 Size: 184 3811 MscI TGG'CCA Cuts at: 1756 1756 Size: 3995 MslI CAynn'nnrTG Cuts at: 260 1984 2111 260 Size: 1724 127 2144 Fragments arranged by size: 2144 1724 127 MspA1I CmG'CkG Cuts at: 1106 1420 1780 2793 3038 1106 Size: 314 360 1013 245 2063 Fragments arranged by size: 2063 1013 360 314 245 NaeI GCC'GGC Cuts at: 2176 2176 Size: 3995 NarI GG'CG_CC Cuts at: 1426 1674 1426 Size: 248 3747 NgoMIV G'CCGG_C Cuts at: 2174 2174 Size: 3995 NspI r_CATG'y Cuts at: 520 1048 1548 2455 520 Size: 528 500 907 2060 Fragments arranged by size: 2060 907 528 500 PciI A'CATG_T Cuts at: 2451 2451 Size: 3995 PmeI GTTT'AAAC Cuts at: 994 994 Size: 3995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3134 3166 3134 Size: 32 3963 PstI C_TGCA'G Cuts at: 1042 1727 1042 Size: 685 3310 PvuI CG_AT'CG Cuts at: 104 104 Size: 3995 PvuII CAG'CTG Cuts at: 1420 1780 1420 Size: 360 3635 RsrII CG'GwC_CG Cuts at: 2190 2190 Size: 3995 SacI G_AGCT'C Cuts at: 1009 1009 Size: 3995 SalI G'TCGA_C Cuts at: 1032 1032 Size: 3995 SapI GCTCTTCn'nnn_ Cuts at: 2018 2228 2018 Size: 210 3785 SbfI CC_TGCA'GG Cuts at: 1042 1042 Size: 3995 ScaI AGT'ACT Cuts at: 953 3512 953 Size: 2559 1436 SfcI C'TryA_G Cuts at: 33 182 211 242 277 978 1038 1723 2716 Size: 149 29 31 35 701 60 685 993 Cuts at: 2716 2907 33 Size: 191 1121 Fragments arranged by size: 1121 993 701 685 191 149 60 35 31 29 SfoI GGC'GCC Cuts at: 1427 1675 1427 Size: 248 3747 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3995 SmaI CCC'GGG Cuts at: 1017 1017 Size: 3995 SmlI C'TyrA_G Cuts at: 370 1150 2065 2557 2819 370 Size: 780 915 492 262 1546 Fragments arranged by size: 1546 915 780 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3995 SphI G_CATG'C Cuts at: 520 1048 1548 520 Size: 528 500 2967 Fragments arranged by size: 2967 528 500 SspI AAT'ATT Cuts at: 855 855 Size: 3995 StyI C'CwwG_G Cuts at: 1129 1483 1129 Size: 354 3641 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 131 1578 1711 2170 2379 131 Size: 1447 133 459 209 1747 Fragments arranged by size: 1747 1447 459 209 133 TatI w'GTAC_w Cuts at: 874 951 3510 874 Size: 77 2559 1359 Fragments arranged by size: 2559 1359 77 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3512 3544 3512 Size: 32 3963 Tth111I GACn'n_nGTC Cuts at: 1792 1792 Size: 3995 XbaI T'CTAG_A Cuts at: 160 1026 160 Size: 866 3129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1490 1490 Size: 3995 XmaI C'CCGG_G Cuts at: 1015 1015 Size: 3995 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI