Restriction map for
pF1KB7115
(Circular) (Six-base) MAPSORT of: pF1KB7115.seq Check: 8870 from: 1 to: 3950
pF1KB7115 3950 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 988 988
Size: 3950
Acc65I G'GTAC_C
Cuts at: 966 966
Size: 3950
AclI AA'CG_TT
Cuts at: 3790 3790
Size: 3950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 2207 2954 3222 1775
Size: 432 747 268 2503
Fragments arranged by size:
2503 747 432 268
AfeI AGC'GCT
Cuts at: 676 676
Size: 3950
AflIII A'CryG_T
Cuts at: 1302 2406 3383 1302
Size: 1104 977 1869
Fragments arranged by size:
1869 1104 977
AgeI A'CCGG_T
Cuts at: 2338 2338
Size: 3950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 325
Size: 3950
AlwNI CAG_nnn'CTG
Cuts at: 192 725 1255 2822 192
Size: 533 530 1567 1320
Fragments arranged by size:
1567 1320 533 530
ApaLI G'TGCA_C
Cuts at: 2720 2720
Size: 3950
ApoI r'AATT_y
Cuts at: 954 3303 3559 954
Size: 2349 256 1345
Fragments arranged by size:
2349 1345 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
AvaI C'yCGr_G
Cuts at: 180 915 970 180
Size: 735 55 3160
Fragments arranged by size:
3160 735 55
BamHI G'GATC_C
Cuts at: 554 975 554
Size: 421 3529
BanI G'GyrC_C
Cuts at: 546 620 966 1380 1628 1663 546
Size: 74 346 414 248 35 2833
Fragments arranged by size:
2833 414 346 248 74 35
BanII G_rGCy'C
Cuts at: 964 1994 964
Size: 1030 2920
BbeI G_GCGC'C
Cuts at: 624 1384 1632 624
Size: 760 248 2942
Fragments arranged by size:
2942 760 248
BciVI GTATCCnnnnn_n'
Cuts at: 1848 2609 3950 1848
Size: 761 1341 1848
Fragments arranged by size:
1848 1341 761
BclI T'GATC_A
Cuts at: 410 1470 410
Size: 1060 2890
BglI GCCn_nnn'nGGC
Cuts at: 808 808
Size: 3950
BglII A'GATC_T
Cuts at: 1465 3502 1465
Size: 2037 1913
BlpI GC'TnA_GC
Cuts at: 1062 1062
Size: 3950
Bme1580I G_kGCm'C
Cuts at: 1575 1668 2724 1575
Size: 93 1056 2801
Fragments arranged by size:
2801 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1162 1243 1578 3722 1162
Size: 81 335 2144 1390
Fragments arranged by size:
2144 1390 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 3950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 388 373
Size: 15 3935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 2005 2497 2795 1126
Size: 879 492 298 2281
Fragments arranged by size:
2281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3654 3654
Size: 3950
BsaAI yAC'GTr
Cuts at: 1303 1303
Size: 3950
BsaBI GATnn'nnATC
Cuts at: 6 1464 3501 6
Size: 1458 2037 455
Fragments arranged by size:
2037 1458 455
BsaHI Gr'CG_yC
Cuts at: 621 1381 1629 2331 3827 621
Size: 760 248 702 1496 744
Fragments arranged by size:
1496 760 744 702 248
BsaWI w'CCGG_w
Cuts at: 1139 1660 2338 2612 2759 3170 1139
Size: 521 678 274 147 411 1919
Fragments arranged by size:
1919 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 835
Size: 30 3920
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 502 775 784 370
Size: 132 273 9 3536
Fragments arranged by size:
3536 273 132 9
BseYI C'CCAG_C
Cuts at: 216 529 746 892 1375 2133 2710 3206 216
Size: 313 217 146 483 758 577 496 960
Fragments arranged by size:
960 758 577 496 483 313 217 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 573 573
Size: 3950
BsiEI CG_ry'CG
Cuts at: 104 446 1492 1538 2746 104
Size: 342 1046 46 1208 1308
Fragments arranged by size:
1308 1208 1046 342 46
BsiHKAI G_wGCw'C
Cuts at: 314 542 667 964 1742 1932 2724 314
Size: 228 125 297 778 190 792 1540
Fragments arranged by size:
1540 792 778 297 228 190 125
BsmBI CGTCTCn'nnnn_
Cuts at: 376 376
Size: 3950
Bsp1286I G_dGCh'C
Cuts at: 314 542 667 964 1575 1668 1742 1932 1994
Size: 228 125 297 611 93 74 190 62
Cuts at: 1994 2724 314
Size: 730 1540
Fragments arranged by size:
1540 730 611 297 228 190 125 93
74 62
BspEI T'CCGG_A
Cuts at: 3170 3170
Size: 3950
BspHI T'CATG_A
Cuts at: 3126 3126
Size: 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1000 1214 1516 1897 1000
Size: 214 302 381 3053
Fragments arranged by size:
3053 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2242 2296 3780 3945 50
Size: 2192 54 1484 165 55
Fragments arranged by size:
2192 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1862 1862
Size: 3950
BsrFI r'CCGG_y
Cuts at: 1948 2129 2338 1948
Size: 181 209 3560
Fragments arranged by size:
3560 209 181
BssSI C'ACGA_G
Cuts at: 615 2221 2579 615
Size: 1606 358 1986
Fragments arranged by size:
1986 1606 358
BstAPI GCAn_nnn'nTGC
Cuts at: 587 725 1327 587
Size: 138 602 3210
Fragments arranged by size:
3210 602 138
BstBI TT'CG_AA
Cuts at: 2311 2311
Size: 3950
BstXI CCAn_nnnn'nTGG
Cuts at: 900 900
Size: 3950
BstYI r'GATC_y
Cuts at: 1 554 975 1442 1465 1800 2046 3058 3144
Size: 553 421 467 23 335 246 1012 86
Cuts at: 3144 3156 3502 1
Size: 12 346 449
Fragments arranged by size:
1012 553 467 449 421 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 515 569 107
Size: 408 54 3488
Fragments arranged by size:
3488 408 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 135 1900 2081 2244 135
Size: 1765 181 163 1841
Fragments arranged by size:
1841 1765 181 163
BtsI GCAGTG_nn'
Cuts at: 164 627 1193 164
Size: 463 566 2921
Fragments arranged by size:
2921 566 463
DraI TTT'AAA
Cuts at: 949 949
Size: 3950
DrdI GACnn_nn'nnGTC
Cuts at: 1656 2514 1656
Size: 858 3092
EaeI y'GGCC_r
Cuts at: 122 419 443 740 1535 1709 2100 2127 3472
Size: 297 24 297 795 174 391 27 1345
Cuts at: 3472 122
Size: 600
Fragments arranged by size:
1345 795 600 391 297 297 174 27
24
EagI C'GGCC_G
Cuts at: 443 1535 443
Size: 1092 2858
EarI CTCTTCn'nnn_
Cuts at: 1973 2183 1973
Size: 210 3740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 781 2158 2468 2614 3760 781
Size: 1377 310 146 1146 971
Fragments arranged by size:
1377 1146 971 310 146
EcoICRI GAG'CTC
Cuts at: 962 962
Size: 3950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 388 1775 2207 2954 3222 373
Size: 15 1387 432 747 268 1101
Fragments arranged by size:
1387 1101 747 432 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 355 355
Size: 3950
EcoO109I rG'GnC_Cy
Cuts at: 176 785 1089 176
Size: 609 304 3037
Fragments arranged by size:
3037 609 304
EcoRI G'AATT_C
Cuts at: 954 954
Size: 3950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 447 660 1969 447
Size: 213 1309 2428
Fragments arranged by size:
2428 1309 213
FspI TGC'GCA
Cuts at: 1731 1731
Size: 3950
HaeII r_GCGC'y
Cuts at: 199 465 624 678 1384 1632 2654 3633 199
Size: 266 159 54 706 248 1022 979 516
Fragments arranged by size:
1022 979 706 516 266 248 159 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 835 867 1792 1822 1824 1854 835
Size: 32 925 30 2 30 2931
Fragments arranged by size:
2931 925 32 30 30 2
HincII GTy'rAC
Cuts at: 989 989
Size: 3950
KasI G'GCGC_C
Cuts at: 620 1380 1628 620
Size: 760 248 2942
Fragments arranged by size:
2942 760 248
KpnI G_GTAC'C
Cuts at: 970 970
Size: 3950
MluI A'CGCG_T
Cuts at: 3383 3383
Size: 3950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 878 2621 2805 878
Size: 1743 184 2023
Fragments arranged by size:
2023 1743 184
MscI TGG'CCA
Cuts at: 124 1711 124
Size: 1587 2363
MslI CAynn'nnrTG
Cuts at: 292 580 1939 2066 292
Size: 288 1359 127 2176
Fragments arranged by size:
2176 1359 288 127
MspA1I CmG'CkG
Cuts at: 192 404 753 1061 1375 1735 2748 2993 192
Size: 212 349 308 314 360 1013 245 1149
Fragments arranged by size:
1149 1013 360 349 314 308 245 212
NaeI GCC'GGC
Cuts at: 2131 2131
Size: 3950
NarI GG'CG_CC
Cuts at: 621 1381 1629 621
Size: 760 248 2942
Fragments arranged by size:
2942 760 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3950
NgoMIV G'CCGG_C
Cuts at: 2129 2129
Size: 3950
NspI r_CATG'y
Cuts at: 585 1003 1503 2410 585
Size: 418 500 907 2125
Fragments arranged by size:
2125 907 500 418
PasI CC'CwG_GG
Cuts at: 790 790
Size: 3950
PciI A'CATG_T
Cuts at: 2406 2406
Size: 3950
PmeI GTTT'AAAC
Cuts at: 949 949
Size: 3950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3918
PpuMI rG'GwC_Cy
Cuts at: 785 785
Size: 3950
PstI C_TGCA'G
Cuts at: 392 721 997 1682 392
Size: 329 276 685 2660
Fragments arranged by size:
2660 685 329 276
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3950
PvuII CAG'CTG
Cuts at: 1375 1735 1375
Size: 360 3590
RsrII CG'GwC_CG
Cuts at: 2145 2145
Size: 3950
SacI G_AGCT'C
Cuts at: 964 964
Size: 3950
SalI G'TCGA_C
Cuts at: 987 987
Size: 3950
SanDI GG'GwC_CC
Cuts at: 785 785
Size: 3950
SapI GCTCTTCn'nnn_
Cuts at: 1973 2183 1973
Size: 210 3740
SbfI CC_TGCA'GG
Cuts at: 997 997
Size: 3950
ScaI AGT'ACT
Cuts at: 3467 3467
Size: 3950
SfcI C'TryA_G
Cuts at: 33 388 717 993 1678 2671 2862 33
Size: 355 329 276 685 993 191 1121
Fragments arranged by size:
1121 993 685 355 329 276 191
SfoI GGC'GCC
Cuts at: 622 1382 1630 622
Size: 760 248 2942
Fragments arranged by size:
2942 760 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3950
SmaI CCC'GGG
Cuts at: 972 972
Size: 3950
SmlI C'TyrA_G
Cuts at: 1105 2020 2512 2774 1105
Size: 915 492 262 2281
Fragments arranged by size:
2281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3950
SphI G_CATG'C
Cuts at: 585 1003 1503 585
Size: 418 500 3032
Fragments arranged by size:
3032 500 418
StyI C'CwwG_G
Cuts at: 107 1084 1438 107
Size: 977 354 2619
Fragments arranged by size:
2619 977 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1533 1666 2125 2334 1533
Size: 133 459 209 3149
Fragments arranged by size:
3149 459 209 133
TatI w'GTAC_w
Cuts at: 3465 3465
Size: 3950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 3467 3499 539
Size: 32 2896 32 990
Fragments arranged by size:
2896 990 32 32
Tth111I GACn'n_nGTC
Cuts at: 1747 1747
Size: 3950
XbaI T'CTAG_A
Cuts at: 981 981
Size: 3950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1445 1445
Size: 3950
XmaI C'CCGG_G
Cuts at: 970 970
Size: 3950
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI