Restriction map for
pF1KB7113
(Circular) (Six-base) MAPSORT of: pF1KB7113.seq Check: 6523 from: 1 to: 3923
pF1KB7113 3923 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 3923
AccI GT'mk_AC
Cuts at: 212 961 212
Size: 749 3174
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1748 2180 2927 3195 878
Size: 870 432 747 268 1606
Fragments arranged by size:
1606 870 747 432 268
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 3891
AlwNI CAG_nnn'CTG
Cuts at: 1228 2795 1228
Size: 1567 2356
ApaI G_GGCC'C
Cuts at: 342 342
Size: 3923
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 927 3276 3532 927
Size: 2349 256 1318
Fragments arranged by size:
2349 1318 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 776 943 776
Size: 167 3756
BamHI G'GATC_C
Cuts at: 861 948 861
Size: 87 3836
BanI G'GyrC_C
Cuts at: 419 685 939 1353 1601 1636 419
Size: 266 254 414 248 35 2706
Fragments arranged by size:
2706 414 266 254 248 35
BanII G_rGCy'C
Cuts at: 342 725 937 1967 342
Size: 383 212 1030 2298
Fragments arranged by size:
2298 1030 383 212
BbeI G_GCGC'C
Cuts at: 689 1357 1605 689
Size: 668 248 3007
Fragments arranged by size:
3007 668 248
BbsI GAAGACnn'nnnn_
Cuts at: 124 189 124
Size: 65 3858
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 767 801 767
Size: 34 3889
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BglI GCCn_nnn'nGGC
Cuts at: 607 607
Size: 3923
BglII A'GATC_T
Cuts at: 1438 3475 1438
Size: 2037 1886
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 342 1548 1641 2697 342
Size: 1206 93 1056 1568
Fragments arranged by size:
1568 1206 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 109
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1978 2470 2768 1099
Size: 879 492 298 2254
Fragments arranged by size:
2254 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 216 390 1276 216
Size: 174 886 2863
Fragments arranged by size:
2863 886 174
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 686 1354 1602 2304 3800 686
Size: 668 248 702 1496 809
Fragments arranged by size:
1496 809 702 668 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 139
Size: 30 3893
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 418 418
Size: 3923
BseYI C'CCAG_C
Cuts at: 850 1348 2106 2683 3179 850
Size: 498 758 577 496 1594
Fragments arranged by size:
1594 758 577 498 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 370 538 613 745 270
Size: 100 168 75 132 3448
Fragments arranged by size:
3448 168 132 100 75
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 725 937 1715 1905 2697 725
Size: 212 778 190 792 1951
Fragments arranged by size:
1951 792 778 212 190
Bsp1286I G_dGCh'C
Cuts at: 342 725 937 1548 1641 1715 1905 1967 2697
Size: 383 212 611 93 74 190 62 730
Cuts at: 2697 342
Size: 1568
Fragments arranged by size:
1568 730 611 383 212 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 3099 3099
Size: 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 557 829 973 1187 1489 1870 557
Size: 272 144 214 302 381 2610
Fragments arranged by size:
2610 381 302 272 214 144
BsrBI CCG'CTC
Cuts at: 50 140 408 2215 2269 3753 3918 50
Size: 90 268 1807 54 1484 165 55
Fragments arranged by size:
1807 1484 268 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 638 1835 638
Size: 1197 2726
BsrFI r'CCGG_y
Cuts at: 416 1921 2102 2311 416
Size: 1505 181 209 2028
Fragments arranged by size:
2028 1505 209 181
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 1300
Size: 3923
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstXI CCAn_nnnn'nTGG
Cuts at: 627 627
Size: 3923
BstYI r'GATC_y
Cuts at: 1 861 948 1415 1438 1773 2019 3031 3117
Size: 860 87 467 23 335 246 1012 86
Cuts at: 3117 3129 3475 1
Size: 12 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 87
86 23 12
BstZ17I GTA'TAC
Cuts at: 213 213
Size: 3923
BtgI C'CryG_G
Cuts at: 107 185 107
Size: 78 3845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 601 1166 601
Size: 565 3358
DraI TTT'AAA
Cuts at: 922 922
Size: 3923
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 1508 1682 2073 2100 3445 1508
Size: 174 391 27 1345 1986
Fragments arranged by size:
1986 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 544 1946 2156 544
Size: 1402 210 2311
Fragments arranged by size:
2311 1402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 681 792 2131 2441 2587 3733 319
Size: 362 111 1339 310 146 1146 509
Fragments arranged by size:
1339 1146 509 362 310 146 111
EcoICRI GAG'CTC
Cuts at: 723 935 723
Size: 212 3711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 109 878 1748 2180 2927 3195 109
Size: 769 870 432 747 268 837
Fragments arranged by size:
870 837 769 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 763 763
Size: 3923
EcoO109I rG'GnC_Cy
Cuts at: 129 339 747 1062 129
Size: 210 408 315 2990
Fragments arranged by size:
2990 408 315 210
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 681 1942 681
Size: 1261 2662
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 417 689 1357 1605 2627 3606 417
Size: 272 668 248 1022 979 734
Fragments arranged by size:
1022 979 734 668 272 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1765 1795 1797 1827 1765
Size: 30 2 30 3861
Fragments arranged by size:
3861 30 30 2
HincII GTy'rAC
Cuts at: 962 962
Size: 3923
KasI G'GCGC_C
Cuts at: 685 1353 1601 685
Size: 668 248 3007
Fragments arranged by size:
3007 668 248
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 2594 2778 347
Size: 2247 184 1492
Fragments arranged by size:
2247 1492 184
MscI TGG'CCA
Cuts at: 1684 1684
Size: 3923
MslI CAynn'nnrTG
Cuts at: 1912 2039 1912
Size: 127 3796
MspA1I CmG'CkG
Cuts at: 827 1034 1348 1708 2721 2966 827
Size: 207 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 360 314 245 207
NaeI GCC'GGC
Cuts at: 418 2104 418
Size: 1686 2237
NarI GG'CG_CC
Cuts at: 686 1354 1602 686
Size: 668 248 3007
Fragments arranged by size:
3007 668 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3923
NgoMIV G'CCGG_C
Cuts at: 416 2102 416
Size: 1686 2237
NspI r_CATG'y
Cuts at: 365 976 1476 2383 365
Size: 611 500 907 1905
Fragments arranged by size:
1905 907 611 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PflMI CCAn_nnn'nTGG
Cuts at: 113 840 113
Size: 727 3196
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PmlI CAC'GTG
Cuts at: 390 390
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 139 171 3062 3094 139
Size: 32 2891 32 968
Fragments arranged by size:
2891 968 32 32
PpuMI rG'GwC_Cy
Cuts at: 747 747
Size: 3923
PspOMI G'GGCC_C
Cuts at: 338 338
Size: 3923
PspXI vC'TCGA_Gb
Cuts at: 776 776
Size: 3923
PstI C_TGCA'G
Cuts at: 228 347 890 970 1655 228
Size: 119 543 80 685 2496
Fragments arranged by size:
2496 685 543 119 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 1348 1708 1348
Size: 360 3563
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 725 937 725
Size: 212 3711
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 154 3440 154
Size: 3286 637
SfcI C'TryA_G
Cuts at: 33 224 343 886 966 1651 2644 2835 33
Size: 191 119 543 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 543 191 191 119 80
SfoI GGC'GCC
Cuts at: 687 1355 1603 687
Size: 668 248 3007
Fragments arranged by size:
3007 668 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 776 1078 1993 2485 2747 776
Size: 302 915 492 262 1952
Fragments arranged by size:
1952 915 492 302 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StuI AGG'CCT
Cuts at: 485 758 485
Size: 273 3650
StyI C'CwwG_G
Cuts at: 107 1057 1411 107
Size: 950 354 2619
Fragments arranged by size:
2619 950 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 1506 1639 2098 2307 915
Size: 591 133 459 209 2531
Fragments arranged by size:
2531 591 459 209 133
TatI w'GTAC_w
Cuts at: 152 3438 152
Size: 3286 637
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XhoI C'TCGA_G
Cuts at: 776 776
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
XmnI GAAnn'nnTTC
Cuts at: 664 664
Size: 3923
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PshAI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI