Restriction map for
pF1KB7103
(Circular) (Six-base) MAPSORT of: pF1KB7103.seq Check: 6395 from: 1 to: 3770
pF1KB7103 3770 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 162 162
Size: 3770
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 1595 2027 2774 3042 681
Size: 914 432 747 268 1409
Fragments arranged by size:
1409 914 747 432 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 1075 2642 1075
Size: 1567 2203
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 774 3123 3379 774
Size: 2349 256 1165
Fragments arranged by size:
2349 1165 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 786 1200 1448 1483 786
Size: 414 248 35 3073
Fragments arranged by size:
3073 414 248 35
BanII G_rGCy'C
Cuts at: 373 583 784 1814 373
Size: 210 201 1030 2329
Fragments arranged by size:
2329 1030 210 201
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglI GCCn_nnn'nGGC
Cuts at: 508 680 508
Size: 172 3598
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 322 1395 1488 2544 322
Size: 1073 93 1056 1548
Fragments arranged by size:
1548 1073 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 982 1063 1398 3542 982
Size: 81 335 2144 1210
Fragments arranged by size:
2144 1210 335 81
BmtI G_CTAG'C
Cuts at: 313 313
Size: 3770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
Bpu10I CC'TnA_GC
Cuts at: 294 294
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 946 1825 2317 2615 110
Size: 836 879 492 298 1265
Fragments arranged by size:
1265 879 836 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 730 3474 730
Size: 2744 1026
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 446 729 1201 1449 2151 3647 446
Size: 283 472 248 702 1496 569
Fragments arranged by size:
1496 702 569 472 283 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 622 652 169
Size: 30 423 30 3287
Fragments arranged by size:
3287 423 30 30
BseYI C'CCAG_C
Cuts at: 191 594 1195 1953 2530 3026 191
Size: 403 601 758 577 496 935
Fragments arranged by size:
935 758 601 577 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 400
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 535 1312 1358 2566 104
Size: 431 777 46 1208 1308
Fragments arranged by size:
1308 1208 777 431 46
BsiHKAI G_wGCw'C
Cuts at: 329 373 784 1562 1752 2544 329
Size: 44 411 778 190 792 1555
Fragments arranged by size:
1555 792 778 411 190 44
Bsp1286I G_dGCh'C
Cuts at: 322 329 373 583 784 1395 1488 1562 1752
Size: 7 44 210 201 611 93 74 190
Cuts at: 1752 1814 2544 322
Size: 62 730 1548
Fragments arranged by size:
1548 730 611 210 201 190 93 74
62 44 7
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 162 820 1034 1336 1717 162
Size: 658 214 302 381 2215
Fragments arranged by size:
2215 658 381 302 214
BsrBI CCG'CTC
Cuts at: 50 293 626 2062 2116 3600 3765 50
Size: 243 333 1436 54 1484 165 55
Fragments arranged by size:
1484 1436 333 243 165 55 54
BsrDI GCAATG_nn'
Cuts at: 560 749 1682 560
Size: 189 933 2648
Fragments arranged by size:
2648 933 189
BsrFI r'CCGG_y
Cuts at: 487 725 1768 1949 2158 487
Size: 238 1043 181 209 2099
Fragments arranged by size:
2099 1043 238 209 181
BssSI C'ACGA_G
Cuts at: 238 451 472 733 2041 2399 238
Size: 213 21 261 1308 358 1609
Fragments arranged by size:
1609 1308 358 261 213 21
BstAPI GCAn_nnn'nTGC
Cuts at: 1147 1147
Size: 3770
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstEII G'GTnAC_C
Cuts at: 483 483
Size: 3770
BstXI CCAn_nnnn'nTGG
Cuts at: 648 648
Size: 3770
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 199 230 675 684 199
Size: 31 445 9 3285
Fragments arranged by size:
3285 445 31 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 268 435 630 1013 268
Size: 167 195 383 3025
Fragments arranged by size:
3025 383 195 167
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DraIII CAC_nnn'GTG
Cuts at: 414 414
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 227 500 509 532 539 650 672 681 723
Size: 273 9 23 7 111 22 9 42
Cuts at: 723 1355 1529 1920 1947 3292 227
Size: 632 174 391 27 1345 705
Fragments arranged by size:
1345 705 632 391 273 174 111 42
27 23 22 9 9 7
EagI C'GGCC_G
Cuts at: 532 1355 532
Size: 823 2947
EarI CTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 1978 2288 2434 3580 388
Size: 1590 310 146 1146 578
Fragments arranged by size:
1590 1146 578 310 146
EcoICRI GAG'CTC
Cuts at: 371 782 371
Size: 411 3359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 1595 2027 2774 3042 681
Size: 914 432 747 268 1409
Fragments arranged by size:
1409 914 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 909 909
Size: 3770
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 427 1551 427
Size: 1124 2646
HaeII r_GCGC'y
Cuts at: 207 214 1204 1452 2474 3453 207
Size: 7 990 248 1022 979 524
Fragments arranged by size:
1022 990 979 524 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 1612 1642 1644 1674 727
Size: 32 853 30 2 30 2823
Fragments arranged by size:
2823 853 32 30 30 2
HincII GTy'rAC
Cuts at: 278 809 278
Size: 531 3239
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 652 674 1531 652
Size: 22 857 2891
Fragments arranged by size:
2891 857 22
MslI CAynn'nnrTG
Cuts at: 646 1759 1886 646
Size: 1113 127 2530
Fragments arranged by size:
2530 1113 127
MspA1I CmG'CkG
Cuts at: 201 363 537 881 1195 1555 2568 2813 201
Size: 162 174 344 314 360 1013 245 1158
Fragments arranged by size:
1158 1013 360 344 314 245 174 162
NaeI GCC'GGC
Cuts at: 727 1951 727
Size: 1224 2546
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NcoI C'CATG_G
Cuts at: 675 684 675
Size: 9 3761
NgoMIV G'CCGG_C
Cuts at: 725 1949 725
Size: 1224 2546
NheI G'CTAG_C
Cuts at: 309 309
Size: 3770
NspI r_CATG'y
Cuts at: 162 823 1323 2230 162
Size: 661 500 907 1702
Fragments arranged by size:
1702 907 661 500
PasI CC'CwG_GG
Cuts at: 133 133
Size: 3770
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PshAI GACnn'nnGTC
Cuts at: 714 714
Size: 3770
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 363 1195 1555 363
Size: 832 360 2578
Fragments arranged by size:
2578 832 360
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 373 784 373
Size: 411 3359
SacII CC_GC'GG
Cuts at: 202 202
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 813 1498 2491 2682 33
Size: 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 508 680 508
Size: 172 3598
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 125 925 1840 2332 2594 125
Size: 800 915 492 262 1301
Fragments arranged by size:
1301 915 800 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
StyI C'CwwG_G
Cuts at: 575 675 684 904 1258 575
Size: 100 9 220 354 3087
Fragments arranged by size:
3087 354 220 100 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 679 1353 1486 1945 2154 607
Size: 72 674 133 459 209 2223
Fragments arranged by size:
2223 674 459 209 133 72
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 169 201 622 654 3287 3319 169
Size: 32 421 32 2633 32 620
Fragments arranged by size:
2633 620 421 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 673 682 1265 552
Size: 121 9 583 3057
Fragments arranged by size:
3057 583 121 9
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BpmI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PfoI
PmlI PpuMI PsiI PspOMI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI