Restriction map for
pF1KB7102
(Circular) (Six-base) MAPSORT of: pF1KB7102.seq Check: 4523 from: 1 to: 3764
pF1KB7102 3764 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AatII G_ACGT'C
Cuts at: 599 599
Size: 3764
AccI GT'mk_AC
Cuts at: 318 802 318
Size: 484 3280
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 1589 2021 2768 3036 189
Size: 1400 432 747 268 917
Fragments arranged by size:
1400 917 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AhdI GACnn_n'nnGTC
Cuts at: 448 448
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 562 1069 2636 562
Size: 507 1567 1690
Fragments arranged by size:
1690 1567 507
ApaI G_GGCC'C
Cuts at: 271 299 271
Size: 28 3736
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 379 489 784 379
Size: 110 295 3359
Fragments arranged by size:
3359 295 110
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 780 1194 1442 1477 780
Size: 414 248 35 3067
Fragments arranged by size:
3067 414 248 35
BanII G_rGCy'C
Cuts at: 233 271 299 778 1808 233
Size: 38 28 479 1030 2189
Fragments arranged by size:
2189 1030 479 38 28
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BbvCI CC'TCA_GC
Cuts at: 563 563
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 678 1662 2423 3764 678
Size: 984 761 1341 678
Fragments arranged by size:
1341 984 761 678
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 271 299 1389 1482 2538 271
Size: 28 1090 93 1056 1497
Fragments arranged by size:
1497 1090 1056 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 274 976 1057 1392 3536 274
Size: 702 81 335 2144 502
Fragments arranged by size:
2144 702 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 412 300
Size: 112 3652
Bpu10I CC'TnA_GC
Cuts at: 563 563
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 596 1195 1443 2145 3641 596
Size: 599 248 702 1496 719
Fragments arranged by size:
1496 719 702 599 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 701 731 701
Size: 30 3734
BseYI C'CCAG_C
Cuts at: 328 1189 1947 2524 3020 328
Size: 861 758 577 496 1072
Fragments arranged by size:
1072 861 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 233 677 778 1556 1746 2538 233
Size: 444 101 778 190 792 1459
Fragments arranged by size:
1459 792 778 444 190 101
BsmBI CGTCTCn'nnnn_
Cuts at: 280 280
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 233 271 299 677 778 1389 1482 1556 1746
Size: 38 28 378 101 611 93 74 190
Cuts at: 1746 1808 2538 233
Size: 62 730 1459
Fragments arranged by size:
1459 730 611 378 190 101 93 74
62 38 28
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 366 1676 366
Size: 1310 2454
BsrFI r'CCGG_y
Cuts at: 588 1762 1943 2152 588
Size: 1174 181 209 2200
Fragments arranged by size:
2200 1174 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstEII G'GTnAC_C
Cuts at: 114 114
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 436 436
Size: 3764
BstYI r'GATC_y
Cuts at: 1 342 529 567 789 1256 1279 1614 1860
Size: 341 187 38 222 467 23 335 246
Cuts at: 1860 2872 2958 2970 3316 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 341 335 246 222
187 86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 421 421
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 563 1007 563
Size: 444 3320
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 516 1349 1523 1914 1941 3286 516
Size: 833 174 391 27 1345 994
Fragments arranged by size:
1345 994 833 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 231 776 231
Size: 545 3219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 189 300 412 1589 2021 2768 3036 189
Size: 111 112 1177 432 747 268 917
Fragments arranged by size:
1177 917 747 432 268 112 111
EcoO109I rG'GnC_Cy
Cuts at: 295 296 624 903 295
Size: 1 328 279 3156
Fragments arranged by size:
3156 328 279 1
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 1198 1446 2468 3447 1198
Size: 248 1022 979 1515
Fragments arranged by size:
1515 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 434 466 1606 1636 1638 1668 434
Size: 32 1140 30 2 30 2530
Fragments arranged by size:
2530 1140 32 30 30 2
HincII GTy'rAC
Cuts at: 319 594 666 803 319
Size: 275 72 137 3280
Fragments arranged by size:
3280 275 137 72
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 372 687 2435 2619 372
Size: 315 1748 184 1517
Fragments arranged by size:
1748 1517 315 184
MscI TGG'CCA
Cuts at: 518 1525 518
Size: 1007 2757
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 875 1189 1549 2562 2807 875
Size: 314 360 1013 245 1832
Fragments arranged by size:
1832 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 613 817 1317 2224 613
Size: 204 500 907 2153
Fragments arranged by size:
2153 907 500 204
PasI CC'CwG_GG
Cuts at: 245 245
Size: 3764
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PpuMI rG'GwC_Cy
Cuts at: 624 624
Size: 3764
PspOMI G'GGCC_C
Cuts at: 267 295 267
Size: 28 3736
PstI C_TGCA'G
Cuts at: 608 811 1496 608
Size: 203 685 2876
Fragments arranged by size:
2876 685 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 1189 1549 1189
Size: 360 3404
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 233 778 233
Size: 545 3219
SalI G'TCGA_C
Cuts at: 317 801 317
Size: 484 3280
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 604 807 1492 2485 2676 33
Size: 571 203 685 993 191 1121
Fragments arranged by size:
1121 993 685 571 203 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 491 786 491
Size: 295 3469
SmlI C'TyrA_G
Cuts at: 919 1834 2326 2588 919
Size: 915 492 262 2095
Fragments arranged by size:
2095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 540 898 1252 540
Size: 358 354 3052
Fragments arranged by size:
3052 358 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 489 784 489
Size: 295 3469
ZraI GAC'GTC
Cuts at: 597 597
Size: 3764
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI