Restriction map for
pF1KB7101
(Circular) (Six-base) MAPSORT of: pF1KB7101.seq Check: 2496 from: 1 to: 3752
pF1KB7101 3752 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 746 3592 746
Size: 2846 906
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 659 1577 2009 2756 3024 343
Size: 316 918 432 747 268 1071
Fragments arranged by size:
1071 918 747 432 316 268
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 720 1057 2624 720
Size: 337 1567 1848
Fragments arranged by size:
1848 1567 337
ApaI G_GGCC'C
Cuts at: 552 552
Size: 3752
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 625 772 625
Size: 147 3605
AvrII C'CTAG_G
Cuts at: 609 609
Size: 3752
BamHI G'GATC_C
Cuts at: 111 527 777 111
Size: 416 250 3086
Fragments arranged by size:
3086 416 250
BanI G'GyrC_C
Cuts at: 613 768 1182 1430 1465 613
Size: 155 414 248 35 2900
Fragments arranged by size:
2900 414 248 155 35
BanII G_rGCy'C
Cuts at: 250 552 766 1796 250
Size: 302 214 1030 2206
Fragments arranged by size:
2206 1030 302 214
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BbsI GAAGACnn'nnnn_
Cuts at: 325 325
Size: 3752
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 593 593
Size: 3752
BglII A'GATC_T
Cuts at: 1267 3304 1267
Size: 2037 1715
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 552 1377 1470 2526 552
Size: 825 93 1056 1778
Fragments arranged by size:
1778 1056 825 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 370 634 103
Size: 267 264 3221
Fragments arranged by size:
3221 267 264
Bpu10I CC'TnA_GC
Cuts at: 244 244
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 928 1807 2299 2597 709
Size: 219 879 492 298 1864
Fragments arranged by size:
1864 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 3456 3456
Size: 3752
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 405 408 552 682 328
Size: 77 3 144 130 3398
Fragments arranged by size:
3398 144 130 77 3
BseYI C'CCAG_C
Cuts at: 262 1177 1935 2512 3008 262
Size: 915 758 577 496 1006
Fragments arranged by size:
1006 915 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 601
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
BsmI GAATG_Cn'
Cuts at: 373 373
Size: 3752
BsmBI CGTCTCn'nnnn_
Cuts at: 565 565
Size: 3752
Bsp1286I G_dGCh'C
Cuts at: 250 552 766 1377 1470 1544 1734 1796 2526
Size: 302 214 611 93 74 190 62 730
Cuts at: 2526 250
Size: 1476
Fragments arranged by size:
1476 730 611 302 214 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 333 2044 2098 3582 3747 50
Size: 283 1711 54 1484 165 55
Fragments arranged by size:
1711 1484 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 2023 2381 2023
Size: 358 3394
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstYI r'GATC_y
Cuts at: 1 111 527 777 1244 1267 1602 1848 2860
Size: 110 416 250 467 23 335 246 1012
Cuts at: 2860 2946 2958 3304 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 416 346 335 250 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 122 1702 1883 2046 122
Size: 1580 181 163 1828
Fragments arranged by size:
1828 1580 181 163
BtsI GCAGTG_nn'
Cuts at: 736 995 736
Size: 259 3493
ClaI AT'CG_AT
Cuts at: 474 474
Size: 3752
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 323 1775 1985 323
Size: 1452 210 2090
Fragments arranged by size:
2090 1452 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 286 1960 2270 2416 3562 286
Size: 1674 310 146 1146 476
Fragments arranged by size:
1674 1146 476 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 343 370 634 659 1577 2009 2756 3024
Size: 240 27 264 25 918 432 747 268
Cuts at: 3024 103
Size: 831
Fragments arranged by size:
918 831 747 432 268 264 240 27
25
EcoO109I rG'GnC_Cy
Cuts at: 548 549 585 891 548
Size: 1 36 306 3409
Fragments arranged by size:
3409 306 36 1
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 196 678 1186 1434 2456 3435 196
Size: 482 508 248 1022 979 513
Fragments arranged by size:
1022 979 513 508 482 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 237 269 1594 1624 1626 1656 237
Size: 32 1325 30 2 30 2333
Fragments arranged by size:
2333 1325 32 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 1741 1868 1741
Size: 127 3625
MspA1I CmG'CkG
Cuts at: 166 303 863 1177 1537 2550 2795 166
Size: 137 560 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 560 360 314 245 137
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 388 805 1305 2212 388
Size: 417 500 907 1928
Fragments arranged by size:
1928 907 500 417
PasI CC'CwG_GG
Cuts at: 532 532
Size: 3752
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PshAI GACnn'nnGTC
Cuts at: 464 464
Size: 3752
PspOMI G'GGCC_C
Cuts at: 548 548
Size: 3752
PstI C_TGCA'G
Cuts at: 171 799 1484 171
Size: 628 685 2439
Fragments arranged by size:
2439 685 628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 166 1177 1537 166
Size: 1011 360 2381
Fragments arranged by size:
2381 1011 360
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 323 1775 1985 323
Size: 1452 210 2090
Fragments arranged by size:
2090 1452 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 167 795 1480 2473 2664 33
Size: 134 628 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 191 134
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 627 774 627
Size: 147 3605
SmlI C'TyrA_G
Cuts at: 724 907 1822 2314 2576 724
Size: 183 915 492 262 1900
Fragments arranged by size:
1900 915 492 262 183
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
SrfI GCCC'GGGC
Cuts at: 627 627
Size: 3752
StyI C'CwwG_G
Cuts at: 107 609 886 1240 107
Size: 502 277 354 2619
Fragments arranged by size:
2619 502 354 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 625 772 625
Size: 147 3605
XmnI GAAnn'nnTTC
Cuts at: 373 475 373
Size: 102 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI