Restriction map for
pF1KB7095
(Circular) (Six-base) MAPSORT of: pF1KB7095.seq Check: 2000 from: 1 to: 3677
pF1KB7095 3677 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 715 715
Size: 3677
Acc65I G'GTAC_C
Cuts at: 312 693 312
Size: 381 3296
AclI AA'CG_TT
Cuts at: 3517 3517
Size: 3677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1502 1934 2681 2949 1502
Size: 432 747 268 2230
Fragments arranged by size:
2230 747 432 268
AflIII A'CryG_T
Cuts at: 500 1029 2133 3110 500
Size: 529 1104 977 1067
Fragments arranged by size:
1104 1067 977 529
AgeI A'CCGG_T
Cuts at: 2065 2065
Size: 3677
AleI CACnn'nnGTG
Cuts at: 120 120
Size: 3677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 3677
AlwNI CAG_nnn'CTG
Cuts at: 982 2549 982
Size: 1567 2110
ApaLI G'TGCA_C
Cuts at: 598 2447 598
Size: 1849 1828
ApoI r'AATT_y
Cuts at: 681 3030 3286 681
Size: 2349 256 1072
Fragments arranged by size:
2349 1072 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
AvaI C'yCGr_G
Cuts at: 697 697
Size: 3677
BamHI G'GATC_C
Cuts at: 702 702
Size: 3677
BanI G'GyrC_C
Cuts at: 312 693 1107 1355 1390 312
Size: 381 414 248 35 2599
Fragments arranged by size:
2599 414 381 248 35
BanII G_rGCy'C
Cuts at: 691 1721 691
Size: 1030 2647
BbeI G_GCGC'C
Cuts at: 1111 1359 1111
Size: 248 3429
BciVI GTATCCnnnnn_n'
Cuts at: 1575 2336 3677 1575
Size: 761 1341 1575
Fragments arranged by size:
1575 1341 761
BclI T'GATC_A
Cuts at: 1197 1197
Size: 3677
BglII A'GATC_T
Cuts at: 1192 3229 1192
Size: 2037 1640
BlpI GC'TnA_GC
Cuts at: 438 789 438
Size: 351 3326
Bme1580I G_kGCm'C
Cuts at: 602 1302 1395 2451 602
Size: 700 93 1056 1828
Fragments arranged by size:
1828 1056 700 93
BmgBI CAC'GTC
Cuts at: 340 340
Size: 3677
BmrI ACTGGGnnnn_n'
Cuts at: 127 610 889 970 1305 3449 127
Size: 483 279 81 335 2144 355
Fragments arranged by size:
2144 483 355 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 3677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1732 2224 2522 853
Size: 879 492 298 2008
Fragments arranged by size:
2008 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3381 3381
Size: 3677
BsaAI yAC'GTr
Cuts at: 503 1030 503
Size: 527 3150
BsaBI GATnn'nnATC
Cuts at: 6 1191 3228 6
Size: 1185 2037 455
Fragments arranged by size:
2037 1185 455
BsaHI Gr'CG_yC
Cuts at: 494 1108 1356 2058 3554 494
Size: 614 248 702 1496 617
Fragments arranged by size:
1496 702 617 614 248
BsaWI w'CCGG_w
Cuts at: 866 1387 2065 2339 2486 2897 866
Size: 521 678 274 147 411 1646
Fragments arranged by size:
1646 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1102 1860 2437 2933 1102
Size: 758 577 496 1846
Fragments arranged by size:
1846 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 3677
BsiEI CG_ry'CG
Cuts at: 104 1219 1265 2473 104
Size: 1115 46 1208 1308
Fragments arranged by size:
1308 1208 1115 46
BsiHKAI G_wGCw'C
Cuts at: 602 691 1469 1659 2451 602
Size: 89 778 190 792 1828
Fragments arranged by size:
1828 792 778 190 89
BsmBI CGTCTCn'nnnn_
Cuts at: 346 346
Size: 3677
Bsp1286I G_dGCh'C
Cuts at: 602 691 1302 1395 1469 1659 1721 2451 602
Size: 89 611 93 74 190 62 730 1828
Fragments arranged by size:
1828 730 611 190 93 89 74 62
BspEI T'CCGG_A
Cuts at: 2897 2897
Size: 3677
BspHI T'CATG_A
Cuts at: 464 2853 464
Size: 2389 1288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 300 727 941 1243 1624 300
Size: 427 214 302 381 2353
Fragments arranged by size:
2353 427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 437 1969 2023 3507 3672 50
Size: 387 1532 54 1484 165 55
Fragments arranged by size:
1532 1484 387 165 55 54
BsrDI GCAATG_nn'
Cuts at: 204 1589 204
Size: 1385 2292
BsrFI r'CCGG_y
Cuts at: 518 1675 1856 2065 518
Size: 1157 181 209 2130
Fragments arranged by size:
2130 1157 209 181
BssSI C'ACGA_G
Cuts at: 120 1948 2306 120
Size: 1828 358 1491
Fragments arranged by size:
1828 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1054 1054
Size: 3677
BstBI TT'CG_AA
Cuts at: 2038 2038
Size: 3677
BstXI CCAn_nnnn'nTGG
Cuts at: 583 583
Size: 3677
BstYI r'GATC_y
Cuts at: 1 702 1169 1192 1527 1773 2785 2871 2883
Size: 701 467 23 335 246 1012 86 12
Cuts at: 2883 3229 1
Size: 346 449
Fragments arranged by size:
1012 701 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 248 248
Size: 3677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1627 1808 1971 1627
Size: 181 163 3333
Fragments arranged by size:
3333 181 163
BtsI GCAGTG_nn'
Cuts at: 920 920
Size: 3677
DraI TTT'AAA
Cuts at: 676 676
Size: 3677
DraIII CAC_nnn'GTG
Cuts at: 384 384
Size: 3677
DrdI GACnn_nn'nnGTC
Cuts at: 1383 2241 1383
Size: 858 2819
EaeI y'GGCC_r
Cuts at: 206 1262 1436 1827 1854 3199 206
Size: 1056 174 391 27 1345 684
Fragments arranged by size:
1345 1056 684 391 174 27
EagI C'GGCC_G
Cuts at: 1262 1262
Size: 3677
EarI CTCTTCn'nnn_
Cuts at: 1700 1910 1700
Size: 210 3467
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1885 2195 2341 3487 1885
Size: 310 146 1146 2075
Fragments arranged by size:
2075 1146 310 146
EcoICRI GAG'CTC
Cuts at: 689 689
Size: 3677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1502 1934 2681 2949 1502
Size: 432 747 268 2230
Fragments arranged by size:
2230 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 816 816
Size: 3677
EcoRI G'AATT_C
Cuts at: 681 681
Size: 3677
EcoRV GAT'ATC
Cuts at: 297 297
Size: 3677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 1696
Size: 3677
FspI TGC'GCA
Cuts at: 1458 1458
Size: 3677
HaeII r_GCGC'y
Cuts at: 1111 1359 2381 3360 1111
Size: 248 1022 979 1428
Fragments arranged by size:
1428 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1519 1549 1551 1581 1519
Size: 30 2 30 3615
Fragments arranged by size:
3615 30 30 2
HincII GTy'rAC
Cuts at: 716 716
Size: 3677
KasI G'GCGC_C
Cuts at: 1107 1355 1107
Size: 248 3429
KpnI G_GTAC'C
Cuts at: 316 697 316
Size: 381 3296
MfeI C'AATT_G
Cuts at: 577 577
Size: 3677
MluI A'CGCG_T
Cuts at: 3110 3110
Size: 3677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 555 2348 2532 555
Size: 1793 184 1700
Fragments arranged by size:
1793 1700 184
MscI TGG'CCA
Cuts at: 1438 1438
Size: 3677
MslI CAynn'nnrTG
Cuts at: 120 1666 1793 120
Size: 1546 127 2004
Fragments arranged by size:
2004 1546 127
MspA1I CmG'CkG
Cuts at: 161 442 542 788 1102 1462 2475 2720 161
Size: 281 100 246 314 360 1013 245 1118
Fragments arranged by size:
1118 1013 360 314 281 246 245 100
NaeI GCC'GGC
Cuts at: 520 1858 520
Size: 1338 2339
NarI GG'CG_CC
Cuts at: 1108 1356 1108
Size: 248 3429
NcoI C'CATG_G
Cuts at: 248 248
Size: 3677
NgoMIV G'CCGG_C
Cuts at: 518 1856 518
Size: 1338 2339
NspI r_CATG'y
Cuts at: 309 730 1230 2137 309
Size: 421 500 907 1849
Fragments arranged by size:
1849 907 500 421
PciI A'CATG_T
Cuts at: 2133 2133
Size: 3677
PflMI CCAn_nnn'nTGG
Cuts at: 384 384
Size: 3677
PmeI GTTT'AAAC
Cuts at: 676 676
Size: 3677
PmlI CAC'GTG
Cuts at: 503 503
Size: 3677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 3645
PstI C_TGCA'G
Cuts at: 724 1409 724
Size: 685 2992
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3677
PvuII CAG'CTG
Cuts at: 442 1102 1462 442
Size: 660 360 2657
Fragments arranged by size:
2657 660 360
RsrII CG'GwC_CG
Cuts at: 1872 1872
Size: 3677
SacI G_AGCT'C
Cuts at: 691 691
Size: 3677
SalI G'TCGA_C
Cuts at: 714 714
Size: 3677
SapI GCTCTTCn'nnn_
Cuts at: 1700 1910 1700
Size: 210 3467
SbfI CC_TGCA'GG
Cuts at: 724 724
Size: 3677
ScaI AGT'ACT
Cuts at: 3194 3194
Size: 3677
SfcI C'TryA_G
Cuts at: 33 720 1405 2398 2589 33
Size: 687 685 993 191 1121
Fragments arranged by size:
1121 993 687 685 191
SfoI GGC'GCC
Cuts at: 1109 1357 1109
Size: 248 3429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
SmaI CCC'GGG
Cuts at: 699 699
Size: 3677
SmlI C'TyrA_G
Cuts at: 832 1747 2239 2501 832
Size: 915 492 262 2008
Fragments arranged by size:
2008 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3677
SphI G_CATG'C
Cuts at: 730 1230 730
Size: 500 3177
StyI C'CwwG_G
Cuts at: 248 811 1165 248
Size: 563 354 2760
Fragments arranged by size:
2760 563 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1260 1393 1852 2061 1260
Size: 133 459 209 2876
Fragments arranged by size:
2876 459 209 133
TatI w'GTAC_w
Cuts at: 302 3192 302
Size: 2890 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 3194 3226 466
Size: 32 2696 32 917
Fragments arranged by size:
2696 917 32 32
Tth111I GACn'n_nGTC
Cuts at: 510 1474 510
Size: 964 2713
XbaI T'CTAG_A
Cuts at: 708 708
Size: 3677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 1172 486
Size: 686 2991
XmaI C'CCGG_G
Cuts at: 697 697
Size: 3677
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI