Restriction map for
pF1KB7091
(Circular) (Six-base) MAPSORT of: pF1KB7091.seq Check: 7745 from: 1 to: 3665
pF1KB7091 3665 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 428 703 428
Size: 275 3390
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1490 1922 2669 2937 102
Size: 1388 432 747 268 830
Fragments arranged by size:
1388 830 747 432 268
AflIII A'CryG_T
Cuts at: 542 1017 2121 3098 542
Size: 475 1104 977 1109
Fragments arranged by size:
1109 1104 977 475
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 387 970 2537 387
Size: 583 1567 1515
Fragments arranged by size:
1567 1515 583
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 397 681 1095 1343 1378 397
Size: 284 414 248 35 2684
Fragments arranged by size:
2684 414 284 248 35
BanII G_rGCy'C
Cuts at: 233 679 1709 233
Size: 446 1030 2189
Fragments arranged by size:
2189 1030 446
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 284 296 549 1185 284
Size: 12 253 636 2764
Fragments arranged by size:
2764 636 253 12
BglI GCCn_nnn'nGGC
Cuts at: 406 406
Size: 3665
BglII A'GATC_T
Cuts at: 617 1180 3217 617
Size: 563 2037 1065
Fragments arranged by size:
2037 1065 563
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 344 877 958 1293 3437 344
Size: 533 81 335 2144 572
Fragments arranged by size:
2144 572 533 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 543 1018 543
Size: 475 3190
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 275 1090 1848 2425 2921 275
Size: 815 758 577 496 1019
Fragments arranged by size:
1019 815 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 3665
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 233 679 1457 1647 2439 233
Size: 446 778 190 792 1459
Fragments arranged by size:
1459 792 778 446 190
Bsp1286I G_dGCh'C
Cuts at: 233 679 1290 1383 1457 1647 1709 2439 233
Size: 446 611 93 74 190 62 730 1459
Fragments arranged by size:
1459 730 611 446 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 339 1577 339
Size: 1238 2427
BsrFI r'CCGG_y
Cuts at: 263 1663 1844 2053 263
Size: 1400 181 209 1875
Fragments arranged by size:
1875 1400 209 181
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstXI CCAn_nnnn'nTGG
Cuts at: 315 357 315
Size: 42 3623
BstYI r'GATC_y
Cuts at: 1 617 690 1157 1180 1515 1761 2773 2859
Size: 616 73 467 23 335 246 1012 86
Cuts at: 2859 2871 3217 1
Size: 12 346 449
Fragments arranged by size:
1012 616 467 449 346 335 246 86
73 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 136 219 908 136
Size: 83 689 2893
Fragments arranged by size:
2893 689 83
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 305 528 1250 1424 1815 1842 3187 305
Size: 223 722 174 391 27 1345 783
Fragments arranged by size:
1345 783 722 391 223 174 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 199 1688 1898 199
Size: 1489 210 1966
Fragments arranged by size:
1966 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 1873 2183 2329 3475 526
Size: 1347 310 146 1146 716
Fragments arranged by size:
1347 1146 716 310 146
EcoICRI GAG'CTC
Cuts at: 231 677 231
Size: 446 3219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 102 1490 1922 2669 2937 102
Size: 1388 432 747 268 830
Fragments arranged by size:
1388 830 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 291 804 291
Size: 513 3152
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 159 191 541 573 1507 1537 1539 1569 159
Size: 32 350 32 934 30 2 30 2255
Fragments arranged by size:
2255 934 350 32 32 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 307 1426 307
Size: 1119 2546
MslI CAynn'nnrTG
Cuts at: 313 332 1654 1781 313
Size: 19 1322 127 2197
Fragments arranged by size:
2197 1322 127 19
MspA1I CmG'CkG
Cuts at: 776 1090 1450 2463 2708 776
Size: 314 360 1013 245 1733
Fragments arranged by size:
1733 1013 360 314 245
NaeI GCC'GGC
Cuts at: 265 1846 265
Size: 1581 2084
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NcoI C'CATG_G
Cuts at: 107 107
Size: 3665
NgoMIV G'CCGG_C
Cuts at: 263 1844 263
Size: 1581 2084
NspI r_CATG'y
Cuts at: 718 1218 2125 718
Size: 500 907 2258
Fragments arranged by size:
2258 907 500
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PmlI CAC'GTG
Cuts at: 543 543
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 494 526 2804 2836 494
Size: 32 2278 32 1323
Fragments arranged by size:
2278 1323 32 32
PpuMI rG'GwC_Cy
Cuts at: 291 291
Size: 3665
PshAI GACnn'nnGTC
Cuts at: 566 566
Size: 3665
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 1090 1450 1090
Size: 360 3305
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 233 679 233
Size: 446 3219
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StyI C'CwwG_G
Cuts at: 107 245 609 799 1153 107
Size: 138 364 190 354 2619
Fragments arranged by size:
2619 364 354 190 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 1248 1381 1840 2049 484
Size: 764 133 459 209 2100
Fragments arranged by size:
2100 764 459 209 133
TatI w'GTAC_w
Cuts at: 251 3180 251
Size: 2929 736
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PmlI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI