Restriction map for
pF1KB7089
(Circular) (Six-base) MAPSORT of: pF1KB7089.seq Check: 6407 from: 1 to: 3569
pF1KB7089 3569 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 607 607
Size: 3569
Acc65I G'GTAC_C
Cuts at: 585 585
Size: 3569
AclI AA'CG_TT
Cuts at: 3409 3409
Size: 3569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1394 1826 2573 2841 1394
Size: 432 747 268 2122
Fragments arranged by size:
2122 747 432 268
AflIII A'CryG_T
Cuts at: 308 921 2025 3002 308
Size: 613 1104 977 875
Fragments arranged by size:
1104 977 875 613
AgeI A'CCGG_T
Cuts at: 1957 1957
Size: 3569
AlwNI CAG_nnn'CTG
Cuts at: 874 2441 874
Size: 1567 2002
ApaLI G'TGCA_C
Cuts at: 2339 2339
Size: 3569
ApoI r'AATT_y
Cuts at: 573 2922 3178 573
Size: 2349 256 964
Fragments arranged by size:
2349 964 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3569
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
AvaI C'yCGr_G
Cuts at: 589 589
Size: 3569
BamHI G'GATC_C
Cuts at: 594 594
Size: 3569
BanI G'GyrC_C
Cuts at: 585 999 1247 1282 585
Size: 414 248 35 2872
Fragments arranged by size:
2872 414 248 35
BanII G_rGCy'C
Cuts at: 518 527 583 1613 518
Size: 9 56 1030 2474
Fragments arranged by size:
2474 1030 56 9
BbeI G_GCGC'C
Cuts at: 1003 1251 1003
Size: 248 3321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 452 418
Size: 34 3535
BciVI GTATCCnnnnn_n'
Cuts at: 1467 2228 3569 1467
Size: 761 1341 1467
Fragments arranged by size:
1467 1341 761
BclI T'GATC_A
Cuts at: 1089 1089
Size: 3569
BglI GCCn_nnn'nGGC
Cuts at: 531 531
Size: 3569
BglII A'GATC_T
Cuts at: 1084 3121 1084
Size: 2037 1532
BlpI GC'TnA_GC
Cuts at: 681 681
Size: 3569
Bme1580I G_kGCm'C
Cuts at: 1194 1287 2343 1194
Size: 93 1056 2420
Fragments arranged by size:
2420 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 781 862 1197 3341 781
Size: 81 335 2144 1009
Fragments arranged by size:
2144 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 513
Size: 3569
Bpu10I CC'TnA_GC
Cuts at: 214 214
Size: 3569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 745 1624 2116 2414 259
Size: 486 879 492 298 1414
Fragments arranged by size:
1414 879 492 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 3273 3273
Size: 3569
BsaAI yAC'GTr
Cuts at: 309 922 309
Size: 613 2956
BsaBI GATnn'nnATC
Cuts at: 6 1083 3120 6
Size: 1077 2037 455
Fragments arranged by size:
2037 1077 455
BsaHI Gr'CG_yC
Cuts at: 482 1000 1248 1950 3446 482
Size: 518 248 702 1496 605
Fragments arranged by size:
1496 702 605 518 248
BsaWI w'CCGG_w
Cuts at: 758 1279 1957 2231 2378 2789 758
Size: 521 678 274 147 411 1538
Fragments arranged by size:
1538 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 994 1752 2329 2825 994
Size: 758 577 496 1738
Fragments arranged by size:
1738 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 210 500 1111 1157 2365 104
Size: 106 290 611 46 1208 1308
Fragments arranged by size:
1308 1208 611 290 106 46
BsiHKAI G_wGCw'C
Cuts at: 200 583 1361 1551 2343 200
Size: 383 778 190 792 1426
Fragments arranged by size:
1426 792 778 383 190
Bsp1286I G_dGCh'C
Cuts at: 200 518 527 583 1194 1287 1361 1551 1613
Size: 318 9 56 611 93 74 190 62
Cuts at: 1613 2343 200
Size: 730 1426
Fragments arranged by size:
1426 730 611 318 190 93 74 62
56 9
BspEI T'CCGG_A
Cuts at: 2789 2789
Size: 3569
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 619 833 1135 1516 619
Size: 214 302 381 2672
Fragments arranged by size:
2672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1861 1915 3399 3564 50
Size: 1811 54 1484 165 55
Fragments arranged by size:
1811 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1481 1481
Size: 3569
BsrFI r'CCGG_y
Cuts at: 1567 1748 1957 1567
Size: 181 209 3179
Fragments arranged by size:
3179 209 181
BssHII G'CGCG_C
Cuts at: 239 241 239
Size: 2 3567
BssSI C'ACGA_G
Cuts at: 1840 2198 1840
Size: 358 3211
BstAPI GCAn_nnn'nTGC
Cuts at: 946 946
Size: 3569
BstBI TT'CG_AA
Cuts at: 1930 1930
Size: 3569
BstXI CCAn_nnnn'nTGG
Cuts at: 393 393
Size: 3569
BstYI r'GATC_y
Cuts at: 1 594 1061 1084 1419 1665 2677 2763 2775
Size: 593 467 23 335 246 1012 86 12
Cuts at: 2775 3121 1
Size: 346 449
Fragments arranged by size:
1012 593 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 485 494 485
Size: 9 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 372 1519 1700 1863 372
Size: 1147 181 163 2078
Fragments arranged by size:
2078 1147 181 163
BtsI GCAGTG_nn'
Cuts at: 812 812
Size: 3569
ClaI AT'CG_AT
Cuts at: 404 404
Size: 3569
DraI TTT'AAA
Cuts at: 568 568
Size: 3569
DrdI GACnn_nn'nnGTC
Cuts at: 1275 2133 1275
Size: 858 2711
EaeI y'GGCC_r
Cuts at: 383 497 1154 1328 1719 1746 3091 383
Size: 114 657 174 391 27 1345 861
Fragments arranged by size:
1345 861 657 391 174 114 27
EagI C'GGCC_G
Cuts at: 497 1154 497
Size: 657 2912
EarI CTCTTCn'nnn_
Cuts at: 172 1592 1802 172
Size: 1420 210 1939
Fragments arranged by size:
1939 1420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1777 2087 2233 3379 1777
Size: 310 146 1146 1967
Fragments arranged by size:
1967 1146 310 146
EcoICRI GAG'CTC
Cuts at: 581 581
Size: 3569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1394 1826 2573 2841 513
Size: 881 432 747 268 1241
Fragments arranged by size:
1241 881 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 708 708
Size: 3569
EcoRI G'AATT_C
Cuts at: 573 573
Size: 3569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1588 1588
Size: 3569
FspI TGC'GCA
Cuts at: 1350 1350
Size: 3569
HaeII r_GCGC'y
Cuts at: 400 1003 1251 2273 3252 400
Size: 603 248 1022 979 717
Fragments arranged by size:
1022 979 717 603 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1411 1441 1443 1473 1411
Size: 30 2 30 3507
Fragments arranged by size:
3507 30 30 2
HincII GTy'rAC
Cuts at: 608 608
Size: 3569
KasI G'GCGC_C
Cuts at: 999 1247 999
Size: 248 3321
KpnI G_GTAC'C
Cuts at: 589 589
Size: 3569
MluI A'CGCG_T
Cuts at: 3002 3002
Size: 3569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 125 230 2240 2424 125
Size: 105 2010 184 1270
Fragments arranged by size:
2010 1270 184 105
MscI TGG'CCA
Cuts at: 1330 1330
Size: 3569
MslI CAynn'nnrTG
Cuts at: 1558 1685 1558
Size: 127 3442
MspA1I CmG'CkG
Cuts at: 356 496 680 994 1354 2367 2612 356
Size: 140 184 314 360 1013 245 1313
Fragments arranged by size:
1313 1013 360 314 245 184 140
NaeI GCC'GGC
Cuts at: 1750 1750
Size: 3569
NarI GG'CG_CC
Cuts at: 1000 1248 1000
Size: 248 3321
NgoMIV G'CCGG_C
Cuts at: 1748 1748
Size: 3569
NotI GC'GGCC_GC
Cuts at: 497 497
Size: 3569
NspI r_CATG'y
Cuts at: 622 1122 2029 622
Size: 500 907 2162
Fragments arranged by size:
2162 907 500
PciI A'CATG_T
Cuts at: 2025 2025
Size: 3569
PmeI GTTT'AAAC
Cuts at: 568 568
Size: 3569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2708 2740 2708
Size: 32 3537
PstI C_TGCA'G
Cuts at: 431 616 1301 431
Size: 185 685 2699
Fragments arranged by size:
2699 685 185
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3569
PvuII CAG'CTG
Cuts at: 994 1354 994
Size: 360 3209
RsrII CG'GwC_CG
Cuts at: 1764 1764
Size: 3569
SacI G_AGCT'C
Cuts at: 583 583
Size: 3569
SacII CC_GC'GG
Cuts at: 497 497
Size: 3569
SalI G'TCGA_C
Cuts at: 606 606
Size: 3569
SapI GCTCTTCn'nnn_
Cuts at: 1592 1802 1592
Size: 210 3359
SbfI CC_TGCA'GG
Cuts at: 431 616 431
Size: 185 3384
ScaI AGT'ACT
Cuts at: 3086 3086
Size: 3569
SfcI C'TryA_G
Cuts at: 33 427 612 1297 2290 2481 33
Size: 394 185 685 993 191 1121
Fragments arranged by size:
1121 993 685 394 191 185
SfoI GGC'GCC
Cuts at: 1001 1249 1001
Size: 248 3321
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3569
SmaI CCC'GGG
Cuts at: 591 591
Size: 3569
SmlI C'TyrA_G
Cuts at: 274 724 1639 2131 2393 274
Size: 450 915 492 262 1450
Fragments arranged by size:
1450 915 492 450 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3569
SphI G_CATG'C
Cuts at: 622 1122 622
Size: 500 3069
StyI C'CwwG_G
Cuts at: 248 703 1057 248
Size: 455 354 2760
Fragments arranged by size:
2760 455 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1152 1285 1744 1953 1152
Size: 133 459 209 2768
Fragments arranged by size:
2768 459 209 133
TatI w'GTAC_w
Cuts at: 311 3084 311
Size: 2773 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3537
Tth111I GACn'n_nGTC
Cuts at: 1366 1366
Size: 3569
XbaI T'CTAG_A
Cuts at: 600 600
Size: 3569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 276 1064 276
Size: 788 2781
XmaI C'CCGG_G
Cuts at: 589 589
Size: 3569
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI