Restriction map for
pF1KB7086
(Circular) (Six-base) MAPSORT of: pF1KB7086.seq Check: 6617 from: 1 to: 3512
pF1KB7086 3512 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 344 528 344
Size: 184 3328
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1769 2516 2784 1337
Size: 432 747 268 2065
Fragments arranged by size:
2065 747 432 268
AflII C'TTAA_G
Cuts at: 364 364
Size: 3512
AflIII A'CryG_T
Cuts at: 864 1968 2945 864
Size: 1104 977 1431
Fragments arranged by size:
1431 1104 977
AgeI A'CCGG_T
Cuts at: 1900 1900
Size: 3512
AlwNI CAG_nnn'CTG
Cuts at: 817 2384 817
Size: 1567 1945
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 516 2865 3121 516
Size: 2349 256 907
Fragments arranged by size:
2349 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 532 532
Size: 3512
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 344 528 942 1190 1225 344
Size: 184 414 248 35 2631
Fragments arranged by size:
2631 414 248 184 35
BanII G_rGCy'C
Cuts at: 526 1556 526
Size: 1030 2482
BbeI G_GCGC'C
Cuts at: 946 1194 946
Size: 248 3264
BbsI GAAGACnn'nnnn_
Cuts at: 426 426
Size: 3512
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 167 1032 167
Size: 865 2647
BglI GCCn_nnn'nGGC
Cuts at: 457 457
Size: 3512
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 1137 1230 2286 1137
Size: 93 1056 2363
Fragments arranged by size:
2363 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 154 1879 154
Size: 1725 1787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1567 2059 2357 688
Size: 879 492 298 1843
Fragments arranged by size:
1843 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3216 3216
Size: 3512
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 943 1191 1893 3389 943
Size: 248 702 1496 1066
Fragments arranged by size:
1496 1066 702 248
BsaWI w'CCGG_w
Cuts at: 701 1222 1900 2174 2321 2732 701
Size: 521 678 274 147 411 1481
Fragments arranged by size:
1481 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 280 460 937 1695 2272 2768 280
Size: 180 477 758 577 496 1024
Fragments arranged by size:
1024 758 577 496 477 180
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 526 1304 1494 2286 526
Size: 778 190 792 1752
Fragments arranged by size:
1752 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 497 497
Size: 3512
Bsp1286I G_dGCh'C
Cuts at: 526 1137 1230 1304 1494 1556 2286 526
Size: 611 93 74 190 62 730 1752
Fragments arranged by size:
1752 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 362 1424 350
Size: 12 1062 2438
Fragments arranged by size:
2438 1062 12
BsrFI r'CCGG_y
Cuts at: 1510 1691 1900 1510
Size: 181 209 3122
Fragments arranged by size:
3122 209 181
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 391 889 391
Size: 498 3014
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstYI r'GATC_y
Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718
Size: 536 467 23 335 246 1012 86 12
Cuts at: 2718 3064 1
Size: 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1462 1643 1806 1462
Size: 181 163 3168
Fragments arranged by size:
3168 181 163
BtsI GCAGTG_nn'
Cuts at: 755 755
Size: 3512
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DraIII CAC_nnn'GTG
Cuts at: 391 391
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 1097 1271 1662 1689 3034 1097
Size: 174 391 27 1345 1575
Fragments arranged by size:
1575 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 166 1535 1745 166
Size: 1369 210 1933
Fragments arranged by size:
1933 1369 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 524 524
Size: 3512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1337 1769 2516 2784 1337
Size: 432 747 268 2065
Fragments arranged by size:
2065 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 651 651
Size: 3512
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 315 390 1531 315
Size: 75 1141 2296
Fragments arranged by size:
2296 1141 75
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 946 1194 2216 3195 946
Size: 248 1022 979 1263
Fragments arranged by size:
1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 473 505 1354 1384 1386 1416 473
Size: 32 849 30 2 30 2569
Fragments arranged by size:
2569 849 32 30 30 2
HincII GTy'rAC
Cuts at: 551 551
Size: 3512
KasI G'GCGC_C
Cuts at: 942 1190 942
Size: 248 3264
KpnI G_GTAC'C
Cuts at: 348 532 348
Size: 184 3328
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 1273 1273
Size: 3512
MslI CAynn'nnrTG
Cuts at: 112 175 355 1501 1628 112
Size: 63 180 1146 127 1996
Fragments arranged by size:
1996 1146 180 127 63
MspA1I CmG'CkG
Cuts at: 623 937 1297 2310 2555 623
Size: 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1693 1693
Size: 3512
NarI GG'CG_CC
Cuts at: 943 1191 943
Size: 248 3264
NgoMIV G'CCGG_C
Cuts at: 1691 1691
Size: 3512
NspI r_CATG'y
Cuts at: 565 1065 1972 565
Size: 500 907 2105
Fragments arranged by size:
2105 907 500
PciI A'CATG_T
Cuts at: 1968 1968
Size: 3512
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PstI C_TGCA'G
Cuts at: 559 1244 559
Size: 685 2827
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 937 1297 937
Size: 360 3152
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 526 526
Size: 3512
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 134 3029 134
Size: 2895 617
SfcI C'TryA_G
Cuts at: 33 555 1240 2233 2424 33
Size: 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 191
SfoI GGC'GCC
Cuts at: 944 1192 944
Size: 248 3264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 364 667 1582 2074 2336 364
Size: 303 915 492 262 1540
Fragments arranged by size:
1540 915 492 303 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 565 1065 565
Size: 500 3012
SspI AAT'ATT
Cuts at: 327 327
Size: 3512
StyI C'CwwG_G
Cuts at: 119 202 646 1000 119
Size: 83 444 354 2631
Fragments arranged by size:
2631 444 354 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1095 1228 1687 1896 1095
Size: 133 459 209 2711
Fragments arranged by size:
2711 459 209 133
TatI w'GTAC_w
Cuts at: 132 3027 132
Size: 2895 617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
XmnI GAAnn'nnTTC
Cuts at: 211 211
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI