Restriction map for
pF1KB7083
(Circular) (Six-base) MAPSORT of: pF1KB7083.seq Check: 3621 from: 1 to: 3491
pF1KB7083 3491 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 529 529
Size: 3491
Acc65I G'GTAC_C
Cuts at: 507 507
Size: 3491
AclI AA'CG_TT
Cuts at: 3331 3331
Size: 3491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1316 1748 2495 2763 265
Size: 1051 432 747 268 993
Fragments arranged by size:
1051 993 747 432 268
AfeI AGC'GCT
Cuts at: 367 367
Size: 3491
AflIII A'CryG_T
Cuts at: 843 1947 2924 843
Size: 1104 977 1410
Fragments arranged by size:
1410 1104 977
AgeI A'CCGG_T
Cuts at: 1879 1879
Size: 3491
AlwNI CAG_nnn'CTG
Cuts at: 796 2363 796
Size: 1567 1924
ApaLI G'TGCA_C
Cuts at: 2261 2261
Size: 3491
ApoI r'AATT_y
Cuts at: 297 495 2844 3100 297
Size: 198 2349 256 688
Fragments arranged by size:
2349 688 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 3491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
AvaI C'yCGr_G
Cuts at: 511 511
Size: 3491
BamHI G'GATC_C
Cuts at: 516 516
Size: 3491
BanI G'GyrC_C
Cuts at: 451 507 921 1169 1204 451
Size: 56 414 248 35 2738
Fragments arranged by size:
2738 414 248 56 35
BanII G_rGCy'C
Cuts at: 151 505 1535 151
Size: 354 1030 2107
Fragments arranged by size:
2107 1030 354
BbeI G_GCGC'C
Cuts at: 925 1173 925
Size: 248 3243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3457
BciVI GTATCCnnnnn_n'
Cuts at: 1389 2150 3491 1389
Size: 761 1341 1389
Fragments arranged by size:
1389 1341 761
BclI T'GATC_A
Cuts at: 1011 1011
Size: 3491
BglII A'GATC_T
Cuts at: 1006 3043 1006
Size: 2037 1454
BlpI GC'TnA_GC
Cuts at: 603 603
Size: 3491
Bme1580I G_kGCm'C
Cuts at: 454 1116 1209 2265 454
Size: 662 93 1056 1680
Fragments arranged by size:
1680 1056 662 93
BmrI ACTGGGnnnn_n'
Cuts at: 703 784 1119 3263 703
Size: 81 335 2144 931
Fragments arranged by size:
2144 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 366
Size: 3491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1546 2038 2336 667
Size: 879 492 298 1822
Fragments arranged by size:
1822 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3195 3195
Size: 3491
BsaAI yAC'GTr
Cuts at: 844 844
Size: 3491
BsaBI GATnn'nnATC
Cuts at: 6 1005 3042 6
Size: 999 2037 455
Fragments arranged by size:
2037 999 455
BsaHI Gr'CG_yC
Cuts at: 922 1170 1872 3368 922
Size: 248 702 1496 1045
Fragments arranged by size:
1496 1045 702 248
BsaWI w'CCGG_w
Cuts at: 680 1201 1879 2153 2300 2711 680
Size: 521 678 274 147 411 1460
Fragments arranged by size:
1460 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 916 1674 2251 2747 916
Size: 758 577 496 1660
Fragments arranged by size:
1660 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 401 1033 1079 2287 104
Size: 297 632 46 1208 1308
Fragments arranged by size:
1308 1208 632 297 46
BsiHKAI G_wGCw'C
Cuts at: 505 1283 1473 2265 505
Size: 778 190 792 1731
Fragments arranged by size:
1731 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 454 505 1116 1209 1283 1473 1535 2265
Size: 303 51 611 93 74 190 62 730
Cuts at: 2265 151
Size: 1377
Fragments arranged by size:
1377 730 611 303 190 93 74 62
51
BspEI T'CCGG_A
Cuts at: 2711 2711
Size: 3491
BspHI T'CATG_A
Cuts at: 293 2667 293
Size: 2374 1117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 240 541 755 1057 1438 240
Size: 301 214 302 381 2293
Fragments arranged by size:
2293 381 302 301 214
BsrBI CCG'CTC
Cuts at: 50 1783 1837 3321 3486 50
Size: 1733 54 1484 165 55
Fragments arranged by size:
1733 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 290 1403 290
Size: 1113 2378
BsrFI r'CCGG_y
Cuts at: 265 1489 1670 1879 265
Size: 1224 181 209 1877
Fragments arranged by size:
1877 1224 209 181
BsrGI T'GTAC_A
Cuts at: 233 233
Size: 3491
BssSI C'ACGA_G
Cuts at: 1762 2120 1762
Size: 358 3133
BstAPI GCAn_nnn'nTGC
Cuts at: 868 868
Size: 3491
BstBI TT'CG_AA
Cuts at: 1852 1852
Size: 3491
BstXI CCAn_nnnn'nTGG
Cuts at: 285 285
Size: 3491
BstYI r'GATC_y
Cuts at: 1 389 516 983 1006 1341 1587 2599 2685
Size: 388 127 467 23 335 246 1012 86
Cuts at: 2685 2697 3043 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 388 346 335 246 127
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 312 1441 1622 1785 312
Size: 1129 181 163 2018
Fragments arranged by size:
2018 1129 181 163
BtsI GCAGTG_nn'
Cuts at: 734 734
Size: 3491
DraI TTT'AAA
Cuts at: 490 490
Size: 3491
DraIII CAC_nnn'GTG
Cuts at: 441 441
Size: 3491
DrdI GACnn_nn'nnGTC
Cuts at: 1197 2055 1197
Size: 858 2633
EaeI y'GGCC_r
Cuts at: 398 428 1076 1250 1641 1668 3013 398
Size: 30 648 174 391 27 1345 876
Fragments arranged by size:
1345 876 648 391 174 30 27
EagI C'GGCC_G
Cuts at: 398 1076 398
Size: 678 2813
EarI CTCTTCn'nnn_
Cuts at: 279 1514 1724 279
Size: 1235 210 2046
Fragments arranged by size:
2046 1235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1699 2009 2155 3301 1699
Size: 310 146 1146 1889
Fragments arranged by size:
1889 1146 310 146
EcoICRI GAG'CTC
Cuts at: 503 503
Size: 3491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 366 1316 1748 2495 2763 265
Size: 101 950 432 747 268 993
Fragments arranged by size:
993 950 747 432 268 101
EcoO109I rG'GnC_Cy
Cuts at: 630 630
Size: 3491
EcoRI G'AATT_C
Cuts at: 297 495 297
Size: 198 3293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1510 1510
Size: 3491
FspI TGC'GCA
Cuts at: 1272 1272
Size: 3491
HaeII r_GCGC'y
Cuts at: 369 925 1173 2195 3174 369
Size: 556 248 1022 979 686
Fragments arranged by size:
1022 979 686 556 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 188 1333 1363 1365 1395 156
Size: 32 1145 30 2 30 2252
Fragments arranged by size:
2252 1145 32 30 30 2
HincII GTy'rAC
Cuts at: 309 530 309
Size: 221 3270
KasI G'GCGC_C
Cuts at: 921 1169 921
Size: 248 3243
KpnI G_GTAC'C
Cuts at: 511 511
Size: 3491
MluI A'CGCG_T
Cuts at: 2924 2924
Size: 3491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 425 2162 2346 425
Size: 1737 184 1570
Fragments arranged by size:
1737 1570 184
MscI TGG'CCA
Cuts at: 430 1252 430
Size: 822 2669
MslI CAynn'nnrTG
Cuts at: 1480 1607 1480
Size: 127 3364
MspA1I CmG'CkG
Cuts at: 602 916 1276 2289 2534 602
Size: 314 360 1013 245 1559
Fragments arranged by size:
1559 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1672 1672
Size: 3491
NarI GG'CG_CC
Cuts at: 922 1170 922
Size: 248 3243
NgoMIV G'CCGG_C
Cuts at: 1670 1670
Size: 3491
NspI r_CATG'y
Cuts at: 544 1044 1951 544
Size: 500 907 2084
Fragments arranged by size:
2084 907 500
PciI A'CATG_T
Cuts at: 1947 1947
Size: 3491
PmeI GTTT'AAAC
Cuts at: 490 490
Size: 3491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 3459
PstI C_TGCA'G
Cuts at: 538 1223 538
Size: 685 2806
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3491
PvuII CAG'CTG
Cuts at: 916 1276 916
Size: 360 3131
RsrII CG'GwC_CG
Cuts at: 1686 1686
Size: 3491
SacI G_AGCT'C
Cuts at: 505 505
Size: 3491
SalI G'TCGA_C
Cuts at: 528 528
Size: 3491
SapI GCTCTTCn'nnn_
Cuts at: 1514 1724 1514
Size: 210 3281
SbfI CC_TGCA'GG
Cuts at: 538 538
Size: 3491
ScaI AGT'ACT
Cuts at: 3008 3008
Size: 3491
SfcI C'TryA_G
Cuts at: 33 534 1219 2212 2403 33
Size: 501 685 993 191 1121
Fragments arranged by size:
1121 993 685 501 191
SfoI GGC'GCC
Cuts at: 923 1171 923
Size: 248 3243
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
SmaI CCC'GGG
Cuts at: 513 513
Size: 3491
SmlI C'TyrA_G
Cuts at: 646 1561 2053 2315 646
Size: 915 492 262 1822
Fragments arranged by size:
1822 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3491
SphI G_CATG'C
Cuts at: 544 1044 544
Size: 500 2991
StyI C'CwwG_G
Cuts at: 278 455 482 625 979 278
Size: 177 27 143 354 2790
Fragments arranged by size:
2790 354 177 143 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1074 1207 1666 1875 1074
Size: 133 459 209 2690
Fragments arranged by size:
2690 459 209 133
TatI w'GTAC_w
Cuts at: 233 470 3006 233
Size: 237 2536 718
Fragments arranged by size:
2536 718 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3459
Tth111I GACn'n_nGTC
Cuts at: 1288 1288
Size: 3491
XbaI T'CTAG_A
Cuts at: 522 522
Size: 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 986 986
Size: 3491
XmaI C'CCGG_G
Cuts at: 511 511
Size: 3491
XmnI GAAnn'nnTTC
Cuts at: 301 301
Size: 3491
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI