Restriction map for
pF1KB7065
(Circular) (Six-base) MAPSORT of: pF1KB7065.seq Check: 9675 from: 1 to: 5462
pF1KB7065 5462 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 2500 2500
Size: 5462
Acc65I G'GTAC_C
Cuts at: 2478 2478
Size: 5462
AclI AA'CG_TT
Cuts at: 5302 5302
Size: 5462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 2237 3287 3719 4466 4734 677
Size: 1560 1050 432 747 268 1405
Fragments arranged by size:
1560 1405 1050 747 432 268
AflIII A'CryG_T
Cuts at: 255 2814 3918 4895 255
Size: 2559 1104 977 822
Fragments arranged by size:
2559 1104 977 822
AgeI A'CCGG_T
Cuts at: 3850 3850
Size: 5462
AleI CACnn'nnGTG
Cuts at: 1934 1934
Size: 5462
AlwNI CAG_nnn'CTG
Cuts at: 2767 4334 2767
Size: 1567 3895
ApaLI G'TGCA_C
Cuts at: 4232 4232
Size: 5462
ApoI r'AATT_y
Cuts at: 228 367 375 479 495 563 899 983 1067
Size: 139 8 104 16 68 336 84 84
Cuts at: 1067 1235 1319 1403 1487 1571 1690 1849 1991
Size: 168 84 84 84 84 119 159 142
Cuts at: 1991 2159 2243 2411 2466 4815 5071 228
Size: 168 84 168 55 2349 256 619
Fragments arranged by size:
2349 619 336 256 168 168 168 159
142 139 119 104 84 84 84 84
84 84 84 68 55 16 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 5462
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
AvaI C'yCGr_G
Cuts at: 2482 2482
Size: 5462
BamHI G'GATC_C
Cuts at: 2487 2487
Size: 5462
BanI G'GyrC_C
Cuts at: 2478 2892 3140 3175 2478
Size: 414 248 35 4765
Fragments arranged by size:
4765 414 248 35
BanII G_rGCy'C
Cuts at: 2476 3506 2476
Size: 1030 4432
BbeI G_GCGC'C
Cuts at: 2896 3144 2896
Size: 248 5214
BbsI GAAGACnn'nnnn_
Cuts at: 402 1454 402
Size: 1052 4410
BciVI GTATCCnnnnn_n'
Cuts at: 3360 4121 5462 3360
Size: 761 1341 3360
Fragments arranged by size:
3360 1341 761
BclI T'GATC_A
Cuts at: 2982 2982
Size: 5462
BglII A'GATC_T
Cuts at: 2977 5014 2977
Size: 2037 3425
BlpI GC'TnA_GC
Cuts at: 2574 2574
Size: 5462
Bme1580I G_kGCm'C
Cuts at: 278 3087 3180 4236 278
Size: 2809 93 1056 1504
Fragments arranged by size:
2809 1504 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 118 2674 2755 3090 5234 118
Size: 2556 81 335 2144 346
Fragments arranged by size:
2556 2144 346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 850 1018 1858 3829 850
Size: 168 840 1971 2483
Fragments arranged by size:
2483 1971 840 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 761 845 1013 1181 1349 1433 1517 1601
Size: 168 84 168 168 168 84 84 84
Cuts at: 1601 1769 1853 1937 2105 2189 2357 2441 593
Size: 168 84 84 168 84 168 84 3614
Fragments arranged by size:
3614 168 168 168 168 168 168 168
84 84 84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2638 3517 4009 4307 2638
Size: 879 492 298 3793
Fragments arranged by size:
3793 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5166 5166
Size: 5462
BsaAI yAC'GTr
Cuts at: 2815 2815
Size: 5462
BsaBI GATnn'nnATC
Cuts at: 6 2976 5013 6
Size: 2970 2037 455
Fragments arranged by size:
2970 2037 455
BsaHI Gr'CG_yC
Cuts at: 2893 3141 3843 5339 2893
Size: 248 702 1496 3016
Fragments arranged by size:
3016 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2651 3172 3850 4124 4271 4682 2651
Size: 521 678 274 147 411 3431
Fragments arranged by size:
3431 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2040 2040
Size: 5462
BseYI C'CCAG_C
Cuts at: 907 2887 3645 4222 4718 907
Size: 1980 758 577 496 1651
Fragments arranged by size:
1980 1651 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1040 2042 2132 1040
Size: 1002 90 4370
Fragments arranged by size:
4370 1002 90
BsiEI CG_ry'CG
Cuts at: 104 3004 3050 4258 104
Size: 2900 46 1208 1308
Fragments arranged by size:
2900 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2476 3254 3444 4236 2476
Size: 778 190 792 3702
Fragments arranged by size:
3702 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 278 2476 3087 3180 3254 3444 3506 4236 278
Size: 2198 611 93 74 190 62 730 1504
Fragments arranged by size:
2198 1504 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4682 4682
Size: 5462
BspHI T'CATG_A
Cuts at: 4638 4638
Size: 5462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2512 2726 3028 3409 2512
Size: 214 302 381 4565
Fragments arranged by size:
4565 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3754 3808 5292 5457 50
Size: 3704 54 1484 165 55
Fragments arranged by size:
3704 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3374 3374
Size: 5462
BsrFI r'CCGG_y
Cuts at: 3460 3641 3850 3460
Size: 181 209 5072
Fragments arranged by size:
5072 209 181
BssSI C'ACGA_G
Cuts at: 3733 4091 3733
Size: 358 5104
BstAPI GCAn_nnn'nTGC
Cuts at: 2839 2839
Size: 5462
BstBI TT'CG_AA
Cuts at: 3823 3823
Size: 5462
BstYI r'GATC_y
Cuts at: 1 2487 2954 2977 3312 3558 4570 4656 4668
Size: 2486 467 23 335 246 1012 86 12
Cuts at: 4668 5014 1
Size: 346 449
Fragments arranged by size:
2486 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3412 3593 3756 3412
Size: 181 163 5118
Fragments arranged by size:
5118 181 163
BtsI GCAGTG_nn'
Cuts at: 2705 2705
Size: 5462
ClaI AT'CG_AT
Cuts at: 1374 1374
Size: 5462
DraI TTT'AAA
Cuts at: 699 1707 2461 699
Size: 1008 754 3700
Fragments arranged by size:
3700 1008 754
DrdI GACnn_nn'nnGTC
Cuts at: 3168 4026 3168
Size: 858 4604
EaeI y'GGCC_r
Cuts at: 157 3047 3221 3612 3639 4984 157
Size: 2890 174 391 27 1345 635
Fragments arranged by size:
2890 1345 635 391 174 27
EagI C'GGCC_G
Cuts at: 3047 3047
Size: 5462
EarI CTCTTCn'nnn_
Cuts at: 653 1073 3485 3695 653
Size: 420 2412 210 2420
Fragments arranged by size:
2420 2412 420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3670 3980 4126 5272 3670
Size: 310 146 1146 3860
Fragments arranged by size:
3860 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2474 2474
Size: 5462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 677 761 845 1013 1181 1349 1433 1517
Size: 84 84 84 168 168 168 84 84
Cuts at: 1517 1601 1769 1853 1937 2105 2189 2237 2357
Size: 84 168 84 84 168 84 48 120
Cuts at: 2357 2441 3287 3719 4466 4734 593
Size: 84 846 432 747 268 1321
Fragments arranged by size:
1321 846 747 432 268 168 168 168
168 168 120 84 84 84 84 84
84 84 84 84 84 48
EcoO109I rG'GnC_Cy
Cuts at: 2601 2601
Size: 5462
EcoRI G'AATT_C
Cuts at: 479 495 563 899 1571 1991 2466 479
Size: 16 68 336 672 420 475 3475
Fragments arranged by size:
3475 672 475 420 336 68 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3481 3481
Size: 5462
FspI TGC'GCA
Cuts at: 3243 3243
Size: 5462
HaeII r_GCGC'y
Cuts at: 2896 3144 4166 5145 2896
Size: 248 1022 979 3213
Fragments arranged by size:
3213 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3304 3334 3336 3366 3304
Size: 30 2 30 5400
Fragments arranged by size:
5400 30 30 2
HincII GTy'rAC
Cuts at: 300 2501 300
Size: 2201 3261
HindIII A'AGCT_T
Cuts at: 860 1028 1196 1280 1364 1532 1868 1952 2120
Size: 168 168 84 84 168 336 84 168
Cuts at: 2120 2204 860
Size: 84 4118
Fragments arranged by size:
4118 336 168 168 168 168 84 84
84 84
HpaI GTT'AAC
Cuts at: 300 300
Size: 5462
KasI G'GCGC_C
Cuts at: 2892 3140 2892
Size: 248 5214
KpnI G_GTAC'C
Cuts at: 2482 2482
Size: 5462
MluI A'CGCG_T
Cuts at: 4895 4895
Size: 5462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4133 4317 4133
Size: 184 5278
MscI TGG'CCA
Cuts at: 159 3223 159
Size: 3064 2398
MslI CAynn'nnrTG
Cuts at: 260 1344 1934 3451 3578 260
Size: 1084 590 1517 127 2144
Fragments arranged by size:
2144 1517 1084 590 127
MspA1I CmG'CkG
Cuts at: 2573 2887 3247 4260 4505 2573
Size: 314 360 1013 245 3530
Fragments arranged by size:
3530 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3643 3643
Size: 5462
NarI GG'CG_CC
Cuts at: 2893 3141 2893
Size: 248 5214
NcoI C'CATG_G
Cuts at: 107 107
Size: 5462
NgoMIV G'CCGG_C
Cuts at: 3641 3641
Size: 5462
NspI r_CATG'y
Cuts at: 259 2515 3015 3922 259
Size: 2256 500 907 1799
Fragments arranged by size:
2256 1799 907 500
PciI A'CATG_T
Cuts at: 255 3918 255
Size: 3663 1799
PmeI GTTT'AAAC
Cuts at: 2461 2461
Size: 5462
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4601 4633 4601
Size: 32 5430
PsiI TTA'TAA
Cuts at: 1958 1958
Size: 5462
PstI C_TGCA'G
Cuts at: 1248 2509 3194 1248
Size: 1261 685 3516
Fragments arranged by size:
3516 1261 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5462
PvuII CAG'CTG
Cuts at: 2887 3247 2887
Size: 360 5102
RsrII CG'GwC_CG
Cuts at: 3657 3657
Size: 5462
SacI G_AGCT'C
Cuts at: 2476 2476
Size: 5462
SalI G'TCGA_C
Cuts at: 2499 2499
Size: 5462
SapI GCTCTTCn'nnn_
Cuts at: 3485 3695 3485
Size: 210 5252
SbfI CC_TGCA'GG
Cuts at: 2509 2509
Size: 5462
ScaI AGT'ACT
Cuts at: 4979 4979
Size: 5462
SfcI C'TryA_G
Cuts at: 33 1244 2505 3190 4183 4374 33
Size: 1211 1261 685 993 191 1121
Fragments arranged by size:
1261 1211 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2894 3142 2894
Size: 248 5214
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5462
SmaI CCC'GGG
Cuts at: 2484 2484
Size: 5462
SmlI C'TyrA_G
Cuts at: 2617 3532 4024 4286 2617
Size: 915 492 262 3793
Fragments arranged by size:
3793 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5462
SphI G_CATG'C
Cuts at: 2515 3015 2515
Size: 500 4962
SspI AAT'ATT
Cuts at: 332 332
Size: 5462
StuI AGG'CCT
Cuts at: 694 706 1702 694
Size: 12 996 4454
Fragments arranged by size:
4454 996 12
StyI C'CwwG_G
Cuts at: 107 2596 2950 107
Size: 2489 354 2619
Fragments arranged by size:
2619 2489 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3045 3178 3637 3846 3045
Size: 133 459 209 4661
Fragments arranged by size:
4661 459 209 133
TatI w'GTAC_w
Cuts at: 4977 4977
Size: 5462
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4979 5011 4979
Size: 32 5430
Tth111I GACn'n_nGTC
Cuts at: 3259 3259
Size: 5462
XbaI T'CTAG_A
Cuts at: 2493 2493
Size: 5462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2957 2957
Size: 5462
XmaI C'CCGG_G
Cuts at: 2482 2482
Size: 5462
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI