Restriction map for
pF1KB7055
(Circular) (Six-base) MAPSORT of: pF1KB7055.seq Check: 2628 from: 1 to: 4859
pF1KB7055 4859 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1359 1359
Size: 4859
AccI GT'mk_AC
Cuts at: 320 1897 320
Size: 1577 3282
Acc65I G'GTAC_C
Cuts at: 1875 1875
Size: 4859
AclI AA'CG_TT
Cuts at: 4699 4699
Size: 4859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 722 1082 1262 1442 2684 3116 3863 4131
Size: 102 360 180 180 1242 432 747 268
Cuts at: 4131 620
Size: 1348
Fragments arranged by size:
1348 1242 747 432 360 268 180 180
102
AflIII A'CryG_T
Cuts at: 2211 3315 4292 2211
Size: 1104 977 2778
Fragments arranged by size:
2778 1104 977
AgeI A'CCGG_T
Cuts at: 3247 3247
Size: 4859
AleI CACnn'nnGTG
Cuts at: 407 407
Size: 4859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1811 1811
Size: 4859
AlwNI CAG_nnn'CTG
Cuts at: 643 2164 3731 643
Size: 1521 1567 1771
Fragments arranged by size:
1771 1567 1521
ApaLI G'TGCA_C
Cuts at: 647 3629 647
Size: 2982 1877
ApoI r'AATT_y
Cuts at: 741 1280 1863 4212 4468 741
Size: 539 583 2349 256 1132
Fragments arranged by size:
2349 1132 583 539 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
AvaI C'yCGr_G
Cuts at: 1879 1879
Size: 4859
AvrII C'CTAG_G
Cuts at: 405 405
Size: 4859
BamHI G'GATC_C
Cuts at: 673 1884 673
Size: 1211 3648
BanI G'GyrC_C
Cuts at: 1875 2289 2537 2572 1875
Size: 414 248 35 4162
Fragments arranged by size:
4162 414 248 35
BanII G_rGCy'C
Cuts at: 887 1873 2903 887
Size: 986 1030 2843
Fragments arranged by size:
2843 1030 986
BbeI G_GCGC'C
Cuts at: 2293 2541 2293
Size: 248 4611
BbsI GAAGACnn'nnnn_
Cuts at: 968 1838 968
Size: 870 3989
BciVI GTATCCnnnnn_n'
Cuts at: 1044 1224 1404 1584 2757 3518 4859 1044
Size: 180 180 180 1173 761 1341 1044
Fragments arranged by size:
1341 1173 1044 761 180 180 180
BclI T'GATC_A
Cuts at: 330 1806 2379 330
Size: 1476 573 2810
Fragments arranged by size:
2810 1476 573
BglII A'GATC_T
Cuts at: 2374 4411 2374
Size: 2037 2822
BlpI GC'TnA_GC
Cuts at: 1971 1971
Size: 4859
Bme1580I G_kGCm'C
Cuts at: 651 2484 2577 3633 651
Size: 1833 93 1056 1877
Fragments arranged by size:
1877 1833 1056 93
BmgBI CAC'GTC
Cuts at: 783 783
Size: 4859
BmrI ACTGGGnnnn_n'
Cuts at: 2071 2152 2487 4631 2071
Size: 81 335 2144 2299
Fragments arranged by size:
2299 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 4859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 1280 2035 2914 3406 3704 521
Size: 759 755 879 492 298 1676
Fragments arranged by size:
1676 879 759 755 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 443 4563 443
Size: 4120 739
BsaAI yAC'GTr
Cuts at: 982 2212 982
Size: 1230 3629
BsaBI GATnn'nnATC
Cuts at: 6 2373 4410 6
Size: 2367 2037 455
Fragments arranged by size:
2367 2037 455
BsaHI Gr'CG_yC
Cuts at: 1356 2290 2538 3240 4736 1356
Size: 934 248 702 1496 1479
Fragments arranged by size:
1496 1479 934 702 248
BsaWI w'CCGG_w
Cuts at: 2048 2569 3247 3521 3668 4079 2048
Size: 521 678 274 147 411 2828
Fragments arranged by size:
2828 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2284 3042 3619 4115 2284
Size: 758 577 496 3028
Fragments arranged by size:
3028 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 812
Size: 4859
BsiEI CG_ry'CG
Cuts at: 104 2401 2447 3655 104
Size: 2297 46 1208 1308
Fragments arranged by size:
2297 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 651 887 1561 1873 2651 2841 3633 651
Size: 236 674 312 778 190 792 1877
Fragments arranged by size:
1877 792 778 674 312 236 190
BsmI GAATG_Cn'
Cuts at: 375 375
Size: 4859
BsmBI CGTCTCn'nnnn_
Cuts at: 1363 1363
Size: 4859
Bsp1286I G_dGCh'C
Cuts at: 651 887 1561 1873 2484 2577 2651 2841 2903
Size: 236 674 312 611 93 74 190 62
Cuts at: 2903 3633 651
Size: 730 1877
Fragments arranged by size:
1877 730 674 611 312 236 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4079 4079
Size: 4859
BspHI T'CATG_A
Cuts at: 333 1725 4035 333
Size: 1392 2310 1157
Fragments arranged by size:
2310 1392 1157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1909 2123 2425 2806 1909
Size: 214 302 381 3962
Fragments arranged by size:
3962 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3151 3205 4689 4854 50
Size: 3101 54 1484 165 55
Fragments arranged by size:
3101 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2771 2771
Size: 4859
BsrFI r'CCGG_y
Cuts at: 2857 3038 3247 2857
Size: 181 209 4469
Fragments arranged by size:
4469 209 181
BsrGI T'GTAC_A
Cuts at: 1020 1380 1020
Size: 360 4499
BssSI C'ACGA_G
Cuts at: 3130 3488 3130
Size: 358 4501
BstAPI GCAn_nnn'nTGC
Cuts at: 2236 2236
Size: 4859
BstBI TT'CG_AA
Cuts at: 3220 3220
Size: 4859
BstYI r'GATC_y
Cuts at: 1 211 673 1884 2351 2374 2709 2955 3967
Size: 210 462 1211 467 23 335 246 1012
Cuts at: 3967 4053 4065 4411 1
Size: 86 12 346 449
Fragments arranged by size:
1211 1012 467 462 449 346 335 246
210 86 23 12
BtgI C'CryG_G
Cuts at: 1005 1005
Size: 4859
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2809 2990 3153 2809
Size: 181 163 4515
Fragments arranged by size:
4515 181 163
BtsI GCAGTG_nn'
Cuts at: 2102 2102
Size: 4859
DraI TTT'AAA
Cuts at: 1858 1858
Size: 4859
DraIII CAC_nnn'GTG
Cuts at: 670 670
Size: 4859
DrdI GACnn_nn'nnGTC
Cuts at: 2565 3423 2565
Size: 858 4001
EaeI y'GGCC_r
Cuts at: 945 1125 1305 1485 1665 2444 2618 3009 3036
Size: 180 180 180 180 779 174 391 27
Cuts at: 3036 4381 945
Size: 1345 1423
Fragments arranged by size:
1423 1345 779 391 180 180 180 180
174 27
EagI C'GGCC_G
Cuts at: 2444 2444
Size: 4859
EarI CTCTTCn'nnn_
Cuts at: 1058 1238 1418 2882 3092 1058
Size: 180 180 1464 210 2825
Fragments arranged by size:
2825 1464 210 180 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3067 3377 3523 4669 3067
Size: 310 146 1146 3257
Fragments arranged by size:
3257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 885 1871 885
Size: 986 3873
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 722 1082 1262 1442 2684 3116 3863 4131
Size: 102 360 180 180 1242 432 747 268
Cuts at: 4131 620
Size: 1348
Fragments arranged by size:
1348 1242 747 432 360 268 180 180
102
EcoNI CCTnn'n_nnAGG
Cuts at: 379 379
Size: 4859
EcoO109I rG'GnC_Cy
Cuts at: 1998 1998
Size: 4859
EcoRI G'AATT_C
Cuts at: 1280 1863 1280
Size: 583 4276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 230 2878 230
Size: 2648 2211
FspI TGC'GCA
Cuts at: 2640 2640
Size: 4859
HaeII r_GCGC'y
Cuts at: 2293 2541 3563 4542 2293
Size: 248 1022 979 2610
Fragments arranged by size:
2610 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 513 545 906 938 1626 1645 1658 1677 2701
Size: 32 361 32 688 19 13 19 1024
Cuts at: 2701 2731 2733 2763 513
Size: 30 2 30 2609
Fragments arranged by size:
2609 1024 688 361 32 32 30 30
19 19 13 2
HincII GTy'rAC
Cuts at: 418 717 780 1898 418
Size: 299 63 1118 3379
Fragments arranged by size:
3379 1118 299 63
HindIII A'AGCT_T
Cuts at: 632 632
Size: 4859
HpaI GTT'AAC
Cuts at: 717 717
Size: 4859
KasI G'GCGC_C
Cuts at: 2289 2537 2289
Size: 248 4611
KpnI G_GTAC'C
Cuts at: 1879 1879
Size: 4859
MfeI C'AATT_G
Cuts at: 199 199
Size: 4859
MluI A'CGCG_T
Cuts at: 4292 4292
Size: 4859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 870 3530 3714 870
Size: 2660 184 2015
Fragments arranged by size:
2660 2015 184
MscI TGG'CCA
Cuts at: 1127 1307 1487 1667 2620 1127
Size: 180 180 180 953 3366
Fragments arranged by size:
3366 953 180 180 180
MslI CAynn'nnrTG
Cuts at: 407 2848 2975 407
Size: 2441 127 2291
Fragments arranged by size:
2441 2291 127
MspA1I CmG'CkG
Cuts at: 166 1970 2284 2644 3657 3902 166
Size: 1804 314 360 1013 245 1123
Fragments arranged by size:
1804 1123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3040 3040
Size: 4859
NarI GG'CG_CC
Cuts at: 2290 2538 2290
Size: 248 4611
NcoI C'CATG_G
Cuts at: 1005 1005
Size: 4859
NgoMIV G'CCGG_C
Cuts at: 3038 3038
Size: 4859
NspI r_CATG'y
Cuts at: 1912 2412 3319 1912
Size: 500 907 3452
Fragments arranged by size:
3452 907 500
PciI A'CATG_T
Cuts at: 3315 3315
Size: 4859
PflMI CCAn_nnn'nTGG
Cuts at: 774 774
Size: 4859
PmeI GTTT'AAAC
Cuts at: 1858 1858
Size: 4859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4827
PsiI TTA'TAA
Cuts at: 434 434
Size: 4859
PstI C_TGCA'G
Cuts at: 1701 1906 2591 1701
Size: 205 685 3969
Fragments arranged by size:
3969 685 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4859
PvuII CAG'CTG
Cuts at: 166 2284 2644 166
Size: 2118 360 2381
Fragments arranged by size:
2381 2118 360
RsrII CG'GwC_CG
Cuts at: 3054 3054
Size: 4859
SacI G_AGCT'C
Cuts at: 887 1873 887
Size: 986 3873
SalI G'TCGA_C
Cuts at: 1896 1896
Size: 4859
SapI GCTCTTCn'nnn_
Cuts at: 1238 2882 3092 1238
Size: 1644 210 3005
Fragments arranged by size:
3005 1644 210
SbfI CC_TGCA'GG
Cuts at: 1906 1906
Size: 4859
ScaI AGT'ACT
Cuts at: 4376 4376
Size: 4859
SexAI A'CCwGG_T
Cuts at: 1091 1271 1451 1091
Size: 180 180 4499
Fragments arranged by size:
4499 180 180
SfcI C'TryA_G
Cuts at: 33 1697 1850 1902 2587 3580 3771 33
Size: 1664 153 52 685 993 191 1121
Fragments arranged by size:
1664 1121 993 685 191 153 52
SfoI GGC'GCC
Cuts at: 2291 2539 2291
Size: 248 4611
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
SmaI CCC'GGG
Cuts at: 1881 1881
Size: 4859
SmlI C'TyrA_G
Cuts at: 536 1295 2014 2929 3421 3683 536
Size: 759 719 915 492 262 1712
Fragments arranged by size:
1712 915 759 719 492 262
SpeI A'CTAG_T
Cuts at: 67 708 67
Size: 641 4218
SphI G_CATG'C
Cuts at: 1912 2412 1912
Size: 500 4359
SspI AAT'ATT
Cuts at: 1773 1773
Size: 4859
StyI C'CwwG_G
Cuts at: 405 1005 1993 2347 405
Size: 600 988 354 2917
Fragments arranged by size:
2917 988 600 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2442 2575 3034 3243 2442
Size: 133 459 209 4058
Fragments arranged by size:
4058 459 209 133
TatI w'GTAC_w
Cuts at: 1020 1162 1342 1380 1522 4374 1020
Size: 142 180 38 142 2852 1505
Fragments arranged by size:
2852 1505 180 142 142 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4376 4408 4376
Size: 32 4827
Tth111I GACn'n_nGTC
Cuts at: 2656 2656
Size: 4859
XbaI T'CTAG_A
Cuts at: 1890 1890
Size: 4859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 226 250 514 2354 226
Size: 24 264 1840 2731
Fragments arranged by size:
2731 1840 264 24
XmaI C'CCGG_G
Cuts at: 1879 1879
Size: 4859
ZraI GAC'GTC
Cuts at: 1357 1357
Size: 4859
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I BsaXI
BseRI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI