Restriction map for
pF1KB7052
(Circular) (Six-base) MAPSORT of: pF1KB7052.seq Check: 2511 from: 1 to: 4730
pF1KB7052 4730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1382 1448 1382
Size: 66 4664
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 1724 2555 2987 3734 4002 1294
Size: 430 831 432 747 268 2022
Fragments arranged by size:
2022 831 747 432 430 268
AflIII A'CryG_T
Cuts at: 2082 3186 4163 2082
Size: 1104 977 2649
Fragments arranged by size:
2649 1104 977
AgeI A'CCGG_T
Cuts at: 3118 3118
Size: 4730
AleI CACnn'nnGTG
Cuts at: 158 206 158
Size: 48 4682
AlwNI CAG_nnn'CTG
Cuts at: 594 886 2035 3602 594
Size: 292 1149 1567 1722
Fragments arranged by size:
1722 1567 1149 292
ApaI G_GGCC'C
Cuts at: 995 1637 1658 995
Size: 642 21 4067
Fragments arranged by size:
4067 642 21
ApaLI G'TGCA_C
Cuts at: 3500 3500
Size: 4730
ApoI r'AATT_y
Cuts at: 418 1734 4083 4339 418
Size: 1316 2349 256 809
Fragments arranged by size:
2349 1316 809 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 118 1750 118
Size: 1632 3098
BamHI G'GATC_C
Cuts at: 1755 1755
Size: 4730
BanI G'GyrC_C
Cuts at: 406 657 774 1086 1490 1683 1708 1746 2160
Size: 251 117 312 404 193 25 38 414
Cuts at: 2160 2408 2443 406
Size: 248 35 2693
Fragments arranged by size:
2693 414 404 312 251 248 193 117
38 35 25
BanII G_rGCy'C
Cuts at: 118 167 585 995 1021 1637 1658 1744 2774
Size: 49 418 410 26 616 21 86 1030
Cuts at: 2774 118
Size: 2074
Fragments arranged by size:
2074 1030 616 418 410 86 49 26
21
BbeI G_GCGC'C
Cuts at: 410 1494 2164 2412 410
Size: 1084 670 248 2728
Fragments arranged by size:
2728 1084 670 248
BbsI GAAGACnn'nnnn_
Cuts at: 647 647
Size: 4730
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3389 4730 2628
Size: 761 1341 2628
Fragments arranged by size:
2628 1341 761
BclI T'GATC_A
Cuts at: 2250 2250
Size: 4730
BglI GCCn_nnn'nGGC
Cuts at: 197 690 197
Size: 493 4237
BglII A'GATC_T
Cuts at: 2245 4282 2245
Size: 2037 2693
BlpI GC'TnA_GC
Cuts at: 1842 1842
Size: 4730
Bme1580I G_kGCm'C
Cuts at: 662 779 788 995 1153 1637 1658 1686 2355
Size: 117 9 207 158 484 21 28 669
Cuts at: 2355 2448 3504 662
Size: 93 1056 1888
Fragments arranged by size:
1888 1056 669 484 207 158 117 93
28 21 9
BmrI ACTGGGnnnn_n'
Cuts at: 359 1640 1942 2023 2358 4502 359
Size: 1281 302 81 335 2144 587
Fragments arranged by size:
2144 1281 587 335 302 81
BmtI G_CTAG'C
Cuts at: 265 265
Size: 4730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1035 3097 1035
Size: 2062 2668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 511 616 644 1492 457
Size: 54 105 28 848 3695
Fragments arranged by size:
3695 848 105 54 28
Bpu10I CC'TnA_GC
Cuts at: 169 1060 169
Size: 891 3839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 1906 2785 3277 3575 1342
Size: 564 879 492 298 2497
Fragments arranged by size:
2497 879 564 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1647 4434 1647
Size: 2787 1943
BsaAI yAC'GTr
Cuts at: 2083 2083
Size: 4730
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 407 1491 2161 2409 3111 4607 407
Size: 1084 670 248 702 1496 530
Fragments arranged by size:
1496 1084 702 670 530 248
BsaWI w'CCGG_w
Cuts at: 221 1919 2440 3118 3392 3539 3950 221
Size: 1698 521 678 274 147 411 1001
Fragments arranged by size:
1698 1001 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 1048 1078 484
Size: 30 534 30 4136
Fragments arranged by size:
4136 534 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 826 829 961 1024 1687 823
Size: 3 3 132 63 663 3866
Fragments arranged by size:
3866 663 132 63 3 3
BseYI C'CCAG_C
Cuts at: 195 553 963 1014 1329 2155 2913 3490 3986
Size: 358 410 51 315 826 758 577 496
Cuts at: 3986 195
Size: 939
Fragments arranged by size:
939 826 758 577 496 410 358 315
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1327 1360 1054
Size: 273 33 4424
Fragments arranged by size:
4424 273 33
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3526 104
Size: 2168 46 1208 1308
Fragments arranged by size:
2168 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 473 1744 2522 2712 3504 167
Size: 306 1271 778 190 792 1393
Fragments arranged by size:
1393 1271 792 778 306 190
Bsp1286I G_dGCh'C
Cuts at: 118 167 473 585 662 779 788 995 1021
Size: 49 306 112 77 117 9 207 26
Cuts at: 1021 1153 1637 1658 1686 1744 2355 2448 2522
Size: 132 484 21 28 58 611 93 74
Cuts at: 2522 2712 2774 3504 118
Size: 190 62 730 1344
Fragments arranged by size:
1344 730 611 484 306 207 190 132
117 112 93 77 74 62 58 49
28 26 21 9
BspEI T'CCGG_A
Cuts at: 221 3950 221
Size: 3729 1001
BspHI T'CATG_A
Cuts at: 3906 3906
Size: 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1382 1448 1780 1994 2296 2677 1382
Size: 66 332 214 302 381 3435
Fragments arranged by size:
3435 381 332 302 214 66
BsrBI CCG'CTC
Cuts at: 50 1130 3022 3076 4560 4725 50
Size: 1080 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 1080 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2642 2642
Size: 4730
BsrFI r'CCGG_y
Cuts at: 364 1388 2728 2909 3118 364
Size: 1024 1340 181 209 1976
Fragments arranged by size:
1976 1340 1024 209 181
BssSI C'ACGA_G
Cuts at: 3001 3359 3001
Size: 358 4372
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2107
Size: 4730
BstBI TT'CG_AA
Cuts at: 3091 3091
Size: 4730
BstYI r'GATC_y
Cuts at: 1 494 1277 1755 2222 2245 2580 2826 3838
Size: 493 783 478 467 23 335 246 1012
Cuts at: 3838 3924 3936 4282 1
Size: 86 12 346 449
Fragments arranged by size:
1012 783 493 478 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 770 899 1142 770
Size: 129 243 4358
Fragments arranged by size:
4358 243 129
BtgI C'CryG_G
Cuts at: 107 614 1173 1712 107
Size: 507 559 539 3125
Fragments arranged by size:
3125 559 539 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 492 2680 2861 3024 492
Size: 2188 181 163 2198
Fragments arranged by size:
2198 2188 181 163
BtsI GCAGTG_nn'
Cuts at: 165 543 846 855 1323 1973 165
Size: 378 303 9 468 650 2922
Fragments arranged by size:
2922 650 468 378 303 9
ClaI AT'CG_AT
Cuts at: 1037 1037
Size: 4730
DraI TTT'AAA
Cuts at: 1729 1729
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3294 2436
Size: 858 3872
EaeI y'GGCC_r
Cuts at: 344 1572 2315 2489 2880 2907 4252 344
Size: 1228 743 174 391 27 1345 822
Fragments arranged by size:
1345 1228 822 743 391 174 27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 938 2753 2963 938
Size: 1815 210 2705
Fragments arranged by size:
2705 1815 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 326 665 1579 2938 3248 3394 4540 326
Size: 339 914 1359 310 146 1146 516
Fragments arranged by size:
1359 1146 914 516 339 310 146
EcoICRI GAG'CTC
Cuts at: 165 1742 165
Size: 1577 3153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 511 616 644 1294 1492 1724 2555 2987
Size: 54 105 28 650 198 232 831 432
Cuts at: 2987 3734 4002 457
Size: 747 268 1185
Fragments arranged by size:
1185 831 747 650 432 268 232 198
105 54 28
EcoNI CCTnn'n_nnAGG
Cuts at: 878 1072 878
Size: 194 4536
EcoO109I rG'GnC_Cy
Cuts at: 231 904 992 1205 1634 1654 1655 1869 231
Size: 673 88 213 429 20 1 214 3092
Fragments arranged by size:
3092 673 429 214 213 88 20 1
EcoRI G'AATT_C
Cuts at: 1734 1734
Size: 4730
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4730
FspI TGC'GCA
Cuts at: 1120 1480 2511 1120
Size: 360 1031 3339
Fragments arranged by size:
3339 1031 360
FspAI rTGC'GCAy
Cuts at: 1480 1480
Size: 4730
HaeII r_GCGC'y
Cuts at: 192 410 455 1308 1449 1494 2164 2412 3434
Size: 218 45 853 141 45 670 248 1022
Cuts at: 3434 4413 192
Size: 979 509
Fragments arranged by size:
1022 979 853 670 509 248 218 141
45 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2572 2602 2604 2634 2572
Size: 30 2 30 4668
Fragments arranged by size:
4668 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
HindIII A'AGCT_T
Cuts at: 289 725 289
Size: 436 4294
KasI G'GCGC_C
Cuts at: 406 1490 2160 2408 406
Size: 1084 670 248 2728
Fragments arranged by size:
2728 1084 670 248
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3401 3585 3401
Size: 184 4546
MscI TGG'CCA
Cuts at: 1574 2491 1574
Size: 917 3813
MslI CAynn'nnrTG
Cuts at: 158 206 2719 2846 158
Size: 48 2513 127 2042
Fragments arranged by size:
2513 2042 127 48
MspA1I CmG'CkG
Cuts at: 195 466 999 1537 1552 1841 2155 2515 3528
Size: 271 533 538 15 289 314 360 1013
Cuts at: 3528 3773 195
Size: 245 1152
Fragments arranged by size:
1152 1013 538 533 360 314 289 271
245 15
NaeI GCC'GGC
Cuts at: 2911 2911
Size: 4730
NarI GG'CG_CC
Cuts at: 407 1491 2161 2409 407
Size: 1084 670 248 2728
Fragments arranged by size:
2728 1084 670 248
NcoI C'CATG_G
Cuts at: 107 1173 1712 107
Size: 1066 539 3125
Fragments arranged by size:
3125 1066 539
NgoMIV G'CCGG_C
Cuts at: 2909 2909
Size: 4730
NheI G'CTAG_C
Cuts at: 261 261
Size: 4730
NspI r_CATG'y
Cuts at: 462 1287 1371 1783 2283 3190 462
Size: 825 84 412 500 907 2002
Fragments arranged by size:
2002 907 825 500 412 84
PasI CC'CwG_GG
Cuts at: 1651 1651
Size: 4730
PciI A'CATG_T
Cuts at: 3186 3186
Size: 4730
PflMI CCAn_nnn'nTGG
Cuts at: 594 970 594
Size: 376 4354
PfoI T'CCnGG_A
Cuts at: 146 146
Size: 4730
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4698
PpuMI rG'GwC_Cy
Cuts at: 904 1205 904
Size: 301 4429
PspOMI G'GGCC_C
Cuts at: 991 1633 1654 991
Size: 642 21 4067
Fragments arranged by size:
4067 642 21
PstI C_TGCA'G
Cuts at: 533 1445 1777 2462 533
Size: 912 332 685 2801
Fragments arranged by size:
2801 912 685 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 195 466 2155 2515 195
Size: 271 1689 360 2410
Fragments arranged by size:
2410 1689 360 271
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 167 1744 167
Size: 1577 3153
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SanDI GG'GwC_CC
Cuts at: 904 904
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 2753 2963 2753
Size: 210 4520
SbfI CC_TGCA'GG
Cuts at: 1777 1777
Size: 4730
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SfcI C'TryA_G
Cuts at: 33 270 529 867 1441 1773 2458 3451 3642
Size: 237 259 338 574 332 685 993 191
Cuts at: 3642 33
Size: 1121
Fragments arranged by size:
1121 993 685 574 338 332 259 237
191
SfoI GGC'GCC
Cuts at: 408 1492 2162 2410 408
Size: 1084 670 248 2728
Fragments arranged by size:
2728 1084 670 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 1752 1752
Size: 4730
SmlI C'TyrA_G
Cuts at: 1357 1885 2800 3292 3554 1357
Size: 528 915 492 262 2533
Fragments arranged by size:
2533 915 528 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 1783 2283 1783
Size: 500 4230
StuI AGG'CCT
Cuts at: 601 1094 601
Size: 493 4237
StyI C'CwwG_G
Cuts at: 107 740 1173 1427 1712 1864 2218 107
Size: 633 433 254 285 152 354 2619
Fragments arranged by size:
2619 633 433 354 285 254 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1701 2313 2446 2905 3114 262
Size: 1439 612 133 459 209 1878
Fragments arranged by size:
1878 1439 612 459 209 133
TatI w'GTAC_w
Cuts at: 4245 4245
Size: 4730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 484 516 4247 4279 484
Size: 32 3731 32 935
Fragments arranged by size:
3731 935 32 32
Tth111I GACn'n_nGTC
Cuts at: 2527 2527
Size: 4730
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1575 2225 1575
Size: 650 4080
XmaI C'CCGG_G
Cuts at: 1750 1750
Size: 4730
XmnI GAAnn'nnTTC
Cuts at: 292 727 292
Size: 435 4295
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII EcoRV
FseI HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI