Restriction map for
pF1KB7051
(Circular) (Six-base) MAPSORT of: pF1KB7051.seq Check: 357 from: 1 to: 4655
pF1KB7051 4655 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 206 1693 206
Size: 1487 3168
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 539 1058 2480 2912 3659 3927 309
Size: 230 519 1422 432 747 268 1037
Fragments arranged by size:
1422 1037 747 519 432 268 230
AflIII A'CryG_T
Cuts at: 1250 1331 2007 3111 4088 1250
Size: 81 676 1104 977 1817
Fragments arranged by size:
1817 1104 977 676 81
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AleI CACnn'nnGTG
Cuts at: 881 1366 881
Size: 485 4170
AlwNI CAG_nnn'CTG
Cuts at: 562 1383 1960 3527 562
Size: 821 577 1567 1690
Fragments arranged by size:
1690 1567 821 577
ApaLI G'TGCA_C
Cuts at: 1200 3425 1200
Size: 2225 2430
ApoI r'AATT_y
Cuts at: 1219 1659 4008 4264 1219
Size: 440 2349 256 1610
Fragments arranged by size:
2349 1610 440 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 571 754 1012 1675 571
Size: 183 258 663 3551
Fragments arranged by size:
3551 663 258 183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 926 959 926
Size: 33 4622
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 363 1671 2085 2333 2368 363
Size: 1308 414 248 35 2650
Fragments arranged by size:
2650 1308 414 248 35
BanII G_rGCy'C
Cuts at: 1669 2699 1669
Size: 1030 3625
BbeI G_GCGC'C
Cuts at: 367 2089 2337 367
Size: 1722 248 2685
Fragments arranged by size:
2685 1722 248
BbvCI CC'TCA_GC
Cuts at: 368 368
Size: 4655
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglI GCCn_nnn'nGGC
Cuts at: 1318 1318
Size: 4655
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 155 1767 155
Size: 1612 3043
Bme1580I G_kGCm'C
Cuts at: 1204 2280 2373 3429 1204
Size: 1076 93 1056 2430
Fragments arranged by size:
2430 1076 1056 93
BmgBI CAC'GTC
Cuts at: 822 822
Size: 4655
BmrI ACTGGGnnnn_n'
Cuts at: 362 1597 1867 1948 2283 4427 362
Size: 1235 270 81 335 2144 590
Fragments arranged by size:
2144 1235 590 335 270 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 701 910 1174 1260 582
Size: 119 209 264 86 3977
Fragments arranged by size:
3977 264 209 119 86
Bpu10I CC'TnA_GC
Cuts at: 368 368
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 757 4359 757
Size: 3602 1053
BsaAI yAC'GTr
Cuts at: 916 1332 2008 916
Size: 416 676 3563
Fragments arranged by size:
3563 676 416
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 364 2086 2334 3036 4532 364
Size: 1722 248 702 1496 487
Fragments arranged by size:
1722 1496 702 487 248
BsaWI w'CCGG_w
Cuts at: 902 1844 2365 3043 3317 3464 3875 902
Size: 942 521 678 274 147 411 1682
Fragments arranged by size:
1682 942 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 740 775 740
Size: 35 4620
BseYI C'CCAG_C
Cuts at: 641 2080 2838 3415 3911 641
Size: 1439 758 577 496 1385
Fragments arranged by size:
1439 1385 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 397 1302 1377 168
Size: 229 905 75 3446
Fragments arranged by size:
3446 905 229 75
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1669 2447 2637 3429 1204
Size: 465 778 190 792 2430
Fragments arranged by size:
2430 792 778 465 190
BsmI GAATG_Cn'
Cuts at: 509 509
Size: 4655
Bsp1286I G_dGCh'C
Cuts at: 1204 1669 2280 2373 2447 2637 2699 3429 1204
Size: 465 611 93 74 190 62 730 2430
Fragments arranged by size:
2430 730 611 465 190 93 74 62
BspEI T'CCGG_A
Cuts at: 902 3875 902
Size: 2973 1682
BspHI T'CATG_A
Cuts at: 836 3831 836
Size: 2995 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 606 1705 1919 2221 2602 606
Size: 1099 214 302 381 2659
Fragments arranged by size:
2659 1099 381 302 214
BsrBI CCG'CTC
Cuts at: 50 426 2947 3001 4485 4650 50
Size: 376 2521 54 1484 165 55
Fragments arranged by size:
2521 1484 376 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1005 1133 2567 1005
Size: 128 1434 3093
Fragments arranged by size:
3093 1434 128
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 347 1253 347
Size: 906 3749
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2032
Size: 4655
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstXI CCAn_nnnn'nTGG
Cuts at: 114 883 1563 114
Size: 769 680 3206
Fragments arranged by size:
3206 769 680
BstYI r'GATC_y
Cuts at: 1 579 929 1680 2147 2170 2505 2751 3763
Size: 578 350 751 467 23 335 246 1012
Cuts at: 3763 3849 3861 4207 1
Size: 86 12 346 449
Fragments arranged by size:
1012 751 578 467 449 350 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 207 207
Size: 4655
Bsu36I CC'TnA_GG
Cuts at: 623 623
Size: 4655
BtgI C'CryG_G
Cuts at: 107 123 583 1025 1364 107
Size: 16 460 442 339 3398
Fragments arranged by size:
3398 460 442 339 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 215 2605 2786 2949 215
Size: 2390 181 163 1921
Fragments arranged by size:
2390 1921 181 163
BtsI GCAGTG_nn'
Cuts at: 1575 1898 1575
Size: 323 4332
ClaI AT'CG_AT
Cuts at: 1094 1094
Size: 4655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 531 566 531
Size: 35 4620
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DraIII CAC_nnn'GTG
Cuts at: 1605 1605
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 711 2361 3219 711
Size: 1650 858 2147
Fragments arranged by size:
2147 1650 858
EaeI y'GGCC_r
Cuts at: 116 586 2240 2414 2805 2832 4177 116
Size: 470 1654 174 391 27 1345 594
Fragments arranged by size:
1654 1345 594 470 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 835 1177 2678 2888 835
Size: 342 1501 210 2602
Fragments arranged by size:
2602 1501 342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1203 2863 3173 3319 4465 1203
Size: 1660 310 146 1146 1393
Fragments arranged by size:
1660 1393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 539 582 701 910 1058 1174 1260 2480
Size: 230 43 119 209 148 116 86 1220
Cuts at: 2480 2912 3659 3927 309
Size: 432 747 268 1037
Fragments arranged by size:
1220 1037 747 432 268 230 209 148
119 116 86 43
EcoNI CCTnn'n_nnAGG
Cuts at: 1156 1156
Size: 4655
EcoO109I rG'GnC_Cy
Cuts at: 618 1794 618
Size: 1176 3479
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 367 894 2089 2337 3359 4338 367
Size: 527 1195 248 1022 979 684
Fragments arranged by size:
1195 1022 979 684 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 507 539 639 671 1544 1576 2497
Size: 32 124 32 100 32 873 32 921
Cuts at: 2497 2527 2529 2559 351
Size: 30 2 30 2447
Fragments arranged by size:
2447 921 873 124 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
HindIII A'AGCT_T
Cuts at: 1299 1299
Size: 4655
KasI G'GCGC_C
Cuts at: 363 2085 2333 363
Size: 1722 248 2685
Fragments arranged by size:
2685 1722 248
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1091 3326 3510 1091
Size: 2235 184 2236
Fragments arranged by size:
2236 2235 184
MscI TGG'CCA
Cuts at: 118 2416 118
Size: 2298 2357
MslI CAynn'nnrTG
Cuts at: 881 1030 1366 1429 2644 2771 881
Size: 149 336 63 1215 127 2765
Fragments arranged by size:
2765 1215 336 149 127 63
MspA1I CmG'CkG
Cuts at: 645 1006 1766 2080 2440 3453 3698 645
Size: 361 760 314 360 1013 245 1602
Fragments arranged by size:
1602 1013 760 361 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 364 2086 2334 364
Size: 1722 248 2685
Fragments arranged by size:
2685 1722 248
NcoI C'CATG_G
Cuts at: 107 123 1025 1364 107
Size: 16 902 339 3398
Fragments arranged by size:
3398 902 339 16
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 1254 1428 1708 2208 3115 1254
Size: 174 280 500 907 2794
Fragments arranged by size:
2794 907 500 280 174
PciI A'CATG_T
Cuts at: 1250 3111 1250
Size: 1861 2794
PfoI T'CCnGG_A
Cuts at: 598 598
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PmlI CAC'GTG
Cuts at: 1332 1332
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PpuMI rG'GwC_Cy
Cuts at: 618 618
Size: 4655
PstI C_TGCA'G
Cuts at: 704 1070 1702 2387 704
Size: 366 632 685 2972
Fragments arranged by size:
2972 685 632 366
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 645 1006 2080 2440 645
Size: 361 1074 360 2860
Fragments arranged by size:
2860 1074 361 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 865 4172 865
Size: 3307 1348
SfcI C'TryA_G
Cuts at: 33 700 993 1066 1698 2383 3376 3567 33
Size: 667 293 73 632 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 632 293 191 73
SfoI GGC'GCC
Cuts at: 365 2087 2335 365
Size: 1722 248 2685
Fragments arranged by size:
2685 1722 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 756 1677 756
Size: 921 3734
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
StuI AGG'CCT
Cuts at: 628 628
Size: 4655
StyI C'CwwG_G
Cuts at: 107 123 1025 1364 1789 2143 107
Size: 16 902 339 425 354 2619
Fragments arranged by size:
2619 902 425 354 339 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1616 2238 2371 2830 3039 1616
Size: 622 133 459 209 3232
Fragments arranged by size:
3232 622 459 209 133
TatI w'GTAC_w
Cuts at: 347 863 1253 4170 347
Size: 516 390 2917 832
Fragments arranged by size:
2917 832 516 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 723 755 1038 1070 4172 4204 723
Size: 32 283 32 3102 32 1174
Fragments arranged by size:
3102 1174 283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 4655
XmaI C'CCGG_G
Cuts at: 754 1675 754
Size: 921 3734
XmnI GAAnn'nnTTC
Cuts at: 1273 1273
Size: 4655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI