Restriction map for
pF1KB7050
(Circular) (Six-base) MAPSORT of: pF1KB7050.seq Check: 5309 from: 1 to: 4625
pF1KB7050 4625 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 216 1641 216
Size: 1425 3200
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1581 2450 2882 3629 3897 1581
Size: 869 432 747 268 2309
Fragments arranged by size:
2309 869 747 432 268
AfeI AGC'GCT
Cuts at: 431 448 431
Size: 17 4608
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AlwNI CAG_nnn'CTG
Cuts at: 1930 3497 1930
Size: 1567 3058
ApaLI G'TGCA_C
Cuts at: 483 3395 483
Size: 2912 1713
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 312 340 566 591 1645 312
Size: 28 226 25 1054 3292
Fragments arranged by size:
3292 1054 226 28 25
AvrII C'CTAG_G
Cuts at: 849 849
Size: 4625
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 216 697 1641 2055 2303 2338 216
Size: 481 944 414 248 35 2503
Fragments arranged by size:
2503 944 481 414 248 35
BanII G_rGCy'C
Cuts at: 599 1639 2669 599
Size: 1040 1030 2555
Fragments arranged by size:
2555 1040 1030
BbeI G_GCGC'C
Cuts at: 2059 2307 2059
Size: 248 4377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 474 508 474
Size: 34 4591
BciVI GTATCCnnnnn_n'
Cuts at: 946 2523 3284 4625 946
Size: 1577 761 1341 946
Fragments arranged by size:
1577 1341 946 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 526 526
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 487 2250 2343 3399 487
Size: 1763 93 1056 1713
Fragments arranged by size:
1763 1713 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 703 1837 1918 2253 4397 703
Size: 1134 81 335 2144 931
Fragments arranged by size:
2144 1134 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 974 367
Size: 607 4018
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 723 1062 1801 2680 3172 3470 418
Size: 305 339 739 879 492 298 1573
Fragments arranged by size:
1573 879 739 492 339 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 128 4329 128
Size: 4201 424
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 388 2056 2304 3006 4502 388
Size: 1668 248 702 1496 511
Fragments arranged by size:
1668 1496 702 511 248
BsaWI w'CCGG_w
Cuts at: 1617 1814 2335 3013 3287 3434 3845 1617
Size: 197 521 678 274 147 411 2397
Fragments arranged by size:
2397 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 566 596 566
Size: 30 4595
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 935 1328 568
Size: 367 393 3865
Fragments arranged by size:
3865 393 367
BseYI C'CCAG_C
Cuts at: 240 259 687 2050 2808 3385 3881 240
Size: 19 428 1363 758 577 496 984
Fragments arranged by size:
1363 984 758 577 496 428 19
BsiEI CG_ry'CG
Cuts at: 104 153 256 284 290 2167 2213 3421 104
Size: 49 103 28 6 1877 46 1208 1308
Fragments arranged by size:
1877 1308 1208 103 49 46 28 6
BsiHKAI G_wGCw'C
Cuts at: 487 592 1639 2417 2607 3399 487
Size: 105 1047 778 190 792 1713
Fragments arranged by size:
1713 1047 792 778 190 105
BsmI GAATG_Cn'
Cuts at: 1148 1148
Size: 4625
Bsp1286I G_dGCh'C
Cuts at: 487 592 599 1639 2250 2343 2417 2607 2669
Size: 105 7 1040 611 93 74 190 62
Cuts at: 2669 3399 487
Size: 730 1713
Fragments arranged by size:
1713 1040 730 611 190 105 93 74
62 7
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 1675 1889 2191 2572 222
Size: 1453 214 302 381 2275
Fragments arranged by size:
2275 1453 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 353 2917 2971 4455 4620 50
Size: 64 239 2564 54 1484 165 55
Fragments arranged by size:
2564 1484 239 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 213 333 1136 2623 2804 3013 213
Size: 120 803 1487 181 209 1825
Fragments arranged by size:
1825 1487 803 209 181 120
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 2002 2002
Size: 4625
BstBI TT'CG_AA
Cuts at: 737 2986 737
Size: 2249 2376
BstYI r'GATC_y
Cuts at: 1 1089 1650 2117 2140 2475 2721 3733 3819
Size: 1088 561 467 23 335 246 1012 86
Cuts at: 3819 3831 4177 1
Size: 12 346 449
Fragments arranged by size:
1088 1012 561 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 761 761
Size: 4625
BtgI C'CryG_G
Cuts at: 107 284 382 414 659 107
Size: 177 98 32 245 4073
Fragments arranged by size:
4073 245 177 98 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 1868 1868
Size: 4625
DraI TTT'AAA
Cuts at: 1199 1624 1199
Size: 425 4200
DraIII CAC_nnn'GTG
Cuts at: 124 124
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 150 253 281 287 515 1507 2210 2384 2775
Size: 103 28 6 228 992 703 174 391
Cuts at: 2775 2802 4147 150
Size: 27 1345 628
Fragments arranged by size:
1345 992 703 628 391 228 174 103
28 27 6
EagI C'GGCC_G
Cuts at: 150 253 281 287 2210 150
Size: 103 28 6 1923 2565
Fragments arranged by size:
2565 1923 103 28 6
EarI CTCTTCn'nnn_
Cuts at: 968 1346 1437 2648 2858 968
Size: 378 91 1211 210 2735
Fragments arranged by size:
2735 1211 378 210 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 2833 3143 3289 4435 182
Size: 2651 310 146 1146 372
Fragments arranged by size:
2651 1146 372 310 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 974 1581 2450 2882 3629 3897 367
Size: 607 607 869 432 747 268 1095
Fragments arranged by size:
1095 869 747 607 607 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1400 1400
Size: 4625
EcoO109I rG'GnC_Cy
Cuts at: 986 1764 986
Size: 778 3847
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
EcoRV GAT'ATC
Cuts at: 1101 1101
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 535 2406 535
Size: 1871 2754
HaeII r_GCGC'y
Cuts at: 433 450 504 532 2059 2307 3329 4308 433
Size: 17 54 28 1527 248 1022 979 750
Fragments arranged by size:
1527 1022 979 750 248 54 28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 1391 1423 2467 2497 2499 2529 994
Size: 32 365 32 1044 30 2 30 3090
Fragments arranged by size:
3090 1044 365 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
KasI G'GCGC_C
Cuts at: 2055 2303 2055
Size: 248 4377
KpnI G_GTAC'C
Cuts at: 220 1645 220
Size: 1425 3200
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 944 1090 3296 3480 944
Size: 146 2206 184 2089
Fragments arranged by size:
2206 2089 184 146
MscI TGG'CCA
Cuts at: 2386 2386
Size: 4625
MslI CAynn'nnrTG
Cuts at: 2614 2741 2614
Size: 127 4498
MspA1I CmG'CkG
Cuts at: 280 286 301 384 404 458 1736 2050 2410
Size: 6 15 83 20 54 1278 314 360
Cuts at: 2410 3423 3668 280
Size: 1013 245 1237
Fragments arranged by size:
1278 1237 1013 360 314 245 83 54
20 15 6
NaeI GCC'GGC
Cuts at: 335 1138 2806 335
Size: 803 1668 2154
Fragments arranged by size:
2154 1668 803
NarI GG'CG_CC
Cuts at: 2056 2304 2056
Size: 248 4377
NcoI C'CATG_G
Cuts at: 107 414 107
Size: 307 4318
NgoMIV G'CCGG_C
Cuts at: 333 1136 2804 333
Size: 803 1668 2154
Fragments arranged by size:
2154 1668 803
NotI GC'GGCC_GC
Cuts at: 253 281 287 253
Size: 28 6 4591
Fragments arranged by size:
4591 28 6
NspI r_CATG'y
Cuts at: 1678 2178 3085 1678
Size: 500 907 3218
Fragments arranged by size:
3218 907 500
PasI CC'CwG_GG
Cuts at: 169 169
Size: 4625
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 420 420
Size: 4625
PfoI T'CCnGG_A
Cuts at: 951 951
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 986 986
Size: 4625
PstI C_TGCA'G
Cuts at: 1440 1672 2357 1440
Size: 232 685 3708
Fragments arranged by size:
3708 685 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 2050 2410 2050
Size: 360 4265
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SacII CC_GC'GG
Cuts at: 287 385 287
Size: 98 4527
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 574 1436 1668 2353 3346 3537 33
Size: 541 862 232 685 993 191 1121
Fragments arranged by size:
1121 993 862 685 541 232 191
SfoI GGC'GCC
Cuts at: 2057 2305 2057
Size: 248 4377
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 342 568 1647 342
Size: 226 1079 3320
Fragments arranged by size:
3320 1079 226
SmlI C'TyrA_G
Cuts at: 433 702 1077 1780 2695 3187 3449 433
Size: 269 375 703 915 492 262 1609
Fragments arranged by size:
1609 915 703 492 375 269 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StyI C'CwwG_G
Cuts at: 107 414 849 1052 1759 2113 107
Size: 307 435 203 707 354 2619
Fragments arranged by size:
2619 707 435 354 307 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 152 685 2208 2341 2800 3009 152
Size: 533 1523 133 459 209 1768
Fragments arranged by size:
1768 1523 533 459 209 133
TatI w'GTAC_w
Cuts at: 1592 4140 1592
Size: 2548 2077
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 4142 4174 457
Size: 32 3653 32 908
Fragments arranged by size:
3653 908 32 32
Tth111I GACn'n_nGTC
Cuts at: 1071 2422 1071
Size: 1351 3274
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2120 2120
Size: 4625
XmaI C'CCGG_G
Cuts at: 340 566 1645 340
Size: 226 1079 3320
Fragments arranged by size:
3320 1079 226
XmnI GAAnn'nnTTC
Cuts at: 1148 1148
Size: 4625
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI