Restriction map for
pF1KB7049
(Circular) (Six-base) MAPSORT of: pF1KB7049.seq Check: 3739 from: 1 to: 4589
pF1KB7049 4589 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1627 1627
Size: 4589
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 4429 4429
Size: 4589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1454 1523 1553 2414 2846 3593 3861 175
Size: 1279 69 30 861 432 747 268 903
Fragments arranged by size:
1279 903 861 747 432 268 69 30
AflIII A'CryG_T
Cuts at: 1941 3045 4022 1941
Size: 1104 977 2508
Fragments arranged by size:
2508 1104 977
AgeI A'CCGG_T
Cuts at: 2977 2977
Size: 4589
AhdI GACnn_n'nnGTC
Cuts at: 918 1568 918
Size: 650 3939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 941 941
Size: 4589
AlwNI CAG_nnn'CTG
Cuts at: 1894 3461 1894
Size: 1567 3022
ApaLI G'TGCA_C
Cuts at: 3359 3359
Size: 4589
ApoI r'AATT_y
Cuts at: 1357 1593 3942 4198 1357
Size: 236 2349 256 1748
Fragments arranged by size:
2349 1748 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4589
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 1385 1609 1385
Size: 224 4365
BamHI G'GATC_C
Cuts at: 334 1521 1614 334
Size: 1187 93 3309
Fragments arranged by size:
3309 1187 93
BanI G'GyrC_C
Cuts at: 123 1605 2019 2267 2302 123
Size: 1482 414 248 35 2410
Fragments arranged by size:
2410 1482 414 248 35
BanII G_rGCy'C
Cuts at: 1121 1186 1603 2633 1121
Size: 65 417 1030 3077
Fragments arranged by size:
3077 1030 417 65
BbeI G_GCGC'C
Cuts at: 2023 2271 2023
Size: 248 4341
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4555
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3248 4589 2487
Size: 761 1341 2487
Fragments arranged by size:
2487 1341 761
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglII A'GATC_T
Cuts at: 1498 2104 4141 1498
Size: 606 2037 1946
Fragments arranged by size:
2037 1946 606
BlpI GC'TnA_GC
Cuts at: 1701 1701
Size: 4589
Bme1580I G_kGCm'C
Cuts at: 128 616 2214 2307 3363 128
Size: 488 1598 93 1056 1354
Fragments arranged by size:
1598 1354 1056 488 93
BmrI ACTGGGnnnn_n'
Cuts at: 139 829 1058 1266 1801 1882 2217 4361 139
Size: 690 229 208 535 81 335 2144 367
Fragments arranged by size:
2144 690 535 367 335 229 208 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 1765 2644 3136 3434 730
Size: 1035 879 492 298 1885
Fragments arranged by size:
1885 1035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1576 4293 1576
Size: 2717 1872
BsaAI yAC'GTr
Cuts at: 568 1942 568
Size: 1374 3215
BsaBI GATnn'nnATC
Cuts at: 6 707 2103 4140 6
Size: 701 1396 2037 455
Fragments arranged by size:
2037 1396 701 455
BsaHI Gr'CG_yC
Cuts at: 2020 2268 2970 4466 2020
Size: 248 702 1496 2143
Fragments arranged by size:
2143 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1567 1778 2299 2977 3251 3398 3809 1567
Size: 211 521 678 274 147 411 2347
Fragments arranged by size:
2347 678 521 411 274 211 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 871 901 871
Size: 30 4559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1564 1564
Size: 4589
BseYI C'CCAG_C
Cuts at: 1546 1580 2014 2772 3349 3845 1546
Size: 34 434 758 577 496 2290
Fragments arranged by size:
2290 758 577 496 434 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 4589
BsiEI CG_ry'CG
Cuts at: 104 2131 2177 3385 104
Size: 2027 46 1208 1308
Fragments arranged by size:
2027 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1121 1603 2381 2571 3363 1121
Size: 482 778 190 792 2347
Fragments arranged by size:
2347 792 778 482 190
Bsp1286I G_dGCh'C
Cuts at: 128 616 1121 1186 1603 2214 2307 2381 2571
Size: 488 505 65 417 611 93 74 190
Cuts at: 2571 2633 3363 128
Size: 62 730 1354
Fragments arranged by size:
1354 730 611 505 488 417 190 93
74 65 62
BspEI T'CCGG_A
Cuts at: 3809 3809
Size: 4589
BspHI T'CATG_A
Cuts at: 699 1113 3765 699
Size: 414 2652 1523
Fragments arranged by size:
2652 1523 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1639 1853 2155 2536 1639
Size: 214 302 381 3692
Fragments arranged by size:
3692 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2881 2935 4419 4584 50
Size: 2831 54 1484 165 55
Fragments arranged by size:
2831 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1343 2501 1343
Size: 1158 3431
BsrFI r'CCGG_y
Cuts at: 2587 2768 2977 2587
Size: 181 209 4199
Fragments arranged by size:
4199 209 181
BsrGI T'GTAC_A
Cuts at: 606 669 606
Size: 63 4526
BssSI C'ACGA_G
Cuts at: 2860 3218 2860
Size: 358 4231
BstAPI GCAn_nnn'nTGC
Cuts at: 1966 1966
Size: 4589
BstBI TT'CG_AA
Cuts at: 2950 2950
Size: 4589
BstYI r'GATC_y
Cuts at: 1 334 1498 1521 1614 2081 2104 2439 2685
Size: 333 1164 23 93 467 23 335 246
Cuts at: 2685 3697 3783 3795 4141 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1164 1012 467 449 346 335 333 246
93 86 23 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2539 2720 2883 2539
Size: 181 163 4245
Fragments arranged by size:
4245 181 163
BtsI GCAGTG_nn'
Cuts at: 1832 1832
Size: 4589
DraI TTT'AAA
Cuts at: 1588 1588
Size: 4589
DraIII CAC_nnn'GTG
Cuts at: 1066 1066
Size: 4589
DrdI GACnn_nn'nnGTC
Cuts at: 189 2295 3153 189
Size: 2106 858 1625
Fragments arranged by size:
2106 1625 858
EaeI y'GGCC_r
Cuts at: 991 1132 1170 2174 2348 2739 2766 4111 991
Size: 141 38 1004 174 391 27 1345 1469
Fragments arranged by size:
1469 1345 1004 391 174 141 38 27
EagI C'GGCC_G
Cuts at: 2174 2174
Size: 4589
EarI CTCTTCn'nnn_
Cuts at: 144 674 1412 2612 2822 144
Size: 530 738 1200 210 1911
Fragments arranged by size:
1911 1200 738 530 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 747 2797 3107 3253 4399 747
Size: 2050 310 146 1146 937
Fragments arranged by size:
2050 1146 937 310 146
EcoICRI GAG'CTC
Cuts at: 1119 1601 1119
Size: 482 4107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1454 1523 1553 2414 2846 3593 3861 175
Size: 1279 69 30 861 432 747 268 903
Fragments arranged by size:
1279 903 861 747 432 268 69 30
EcoO109I rG'GnC_Cy
Cuts at: 175 1728 175
Size: 1553 3036
EcoRI G'AATT_C
Cuts at: 1593 1593
Size: 4589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 180 2608 180
Size: 2428 2161
FspI TGC'GCA
Cuts at: 2370 2370
Size: 4589
HaeII r_GCGC'y
Cuts at: 2023 2271 3293 4272 2023
Size: 248 1022 979 2340
Fragments arranged by size:
2340 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 871 903 904 936 1554 1586 2431
Size: 32 253 32 1 32 618 32 845
Cuts at: 2431 2461 2463 2493 586
Size: 30 2 30 2682
Fragments arranged by size:
2682 845 618 253 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 1628 1628
Size: 4589
HindIII A'AGCT_T
Cuts at: 1535 1535
Size: 4589
KasI G'GCGC_C
Cuts at: 2019 2267 2019
Size: 248 4341
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MluI A'CGCG_T
Cuts at: 4022 4022
Size: 4589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3260 3444 3260
Size: 184 4405
MscI TGG'CCA
Cuts at: 1134 1172 2350 1134
Size: 38 1178 3373
Fragments arranged by size:
3373 1178 38
MslI CAynn'nnrTG
Cuts at: 1282 2578 2705 1282
Size: 1296 127 3166
Fragments arranged by size:
3166 1296 127
MspA1I CmG'CkG
Cuts at: 1700 2014 2374 3387 3632 1700
Size: 314 360 1013 245 2657
Fragments arranged by size:
2657 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2770 2770
Size: 4589
NarI GG'CG_CC
Cuts at: 2020 2268 2020
Size: 248 4341
NgoMIV G'CCGG_C
Cuts at: 2768 2768
Size: 4589
NspI r_CATG'y
Cuts at: 744 896 918 1642 2142 3049 744
Size: 152 22 724 500 907 2284
Fragments arranged by size:
2284 907 724 500 152 22
PciI A'CATG_T
Cuts at: 3045 3045
Size: 4589
PflMI CCAn_nnn'nTGG
Cuts at: 254 1020 254
Size: 766 3823
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PmlI CAC'GTG
Cuts at: 568 568
Size: 4589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 928 960 3728 3760 928
Size: 32 2768 32 1757
Fragments arranged by size:
2768 1757 32 32
PstI C_TGCA'G
Cuts at: 1636 2321 1636
Size: 685 3904
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 2014 2374 2014
Size: 360 4229
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 4589
SacI G_AGCT'C
Cuts at: 1121 1603 1121
Size: 482 4107
SalI G'TCGA_C
Cuts at: 1626 1626
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 1412 2612 2822 1412
Size: 1200 210 3179
Fragments arranged by size:
3179 1200 210
SbfI CC_TGCA'GG
Cuts at: 1636 1636
Size: 4589
ScaI AGT'ACT
Cuts at: 511 4106 511
Size: 3595 994
SexAI A'CCwGG_T
Cuts at: 1103 1103
Size: 4589
SfcI C'TryA_G
Cuts at: 33 288 1632 2317 3310 3501 33
Size: 255 1344 685 993 191 1121
Fragments arranged by size:
1344 1121 993 685 255 191
SfoI GGC'GCC
Cuts at: 2021 2269 2021
Size: 248 4341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SmaI CCC'GGG
Cuts at: 1387 1611 1387
Size: 224 4365
SmlI C'TyrA_G
Cuts at: 745 1744 2659 3151 3413 745
Size: 999 915 492 262 1921
Fragments arranged by size:
1921 999 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 744 1642 2142 744
Size: 898 500 3191
Fragments arranged by size:
3191 898 500
StuI AGG'CCT
Cuts at: 851 1268 851
Size: 417 4172
StyI C'CwwG_G
Cuts at: 1723 2077 1723
Size: 354 4235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 2172 2305 2764 2973 1077
Size: 1095 133 459 209 2693
Fragments arranged by size:
2693 1095 459 209 133
TatI w'GTAC_w
Cuts at: 509 606 669 4104 509
Size: 97 63 3435 994
Fragments arranged by size:
3435 994 97 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4106 4138 4106
Size: 32 4557
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 1620 1620
Size: 4589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2084 2084
Size: 4589
XmaI C'CCGG_G
Cuts at: 1385 1609 1385
Size: 224 4365
XmnI GAAnn'nnTTC
Cuts at: 440 440
Size: 4589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI