Restriction map for
pF1KB7047
(Circular) (Six-base) MAPSORT of: pF1KB7047.seq Check: 2600 from: 1 to: 4583
pF1KB7047 4583 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 416 1621 416
Size: 1205 3378
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 1577 4423 1577
Size: 2846 1737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 568 800 1022 1192 1250 1442 2408 2840
Size: 209 232 222 170 58 192 966 432
Cuts at: 2840 3587 3855 359
Size: 747 268 1087
Fragments arranged by size:
1087 966 747 432 268 232 222 209
192 170 58
AfeI AGC'GCT
Cuts at: 1195 1195
Size: 4583
AflIII A'CryG_T
Cuts at: 1935 3039 4016 1935
Size: 1104 977 2502
Fragments arranged by size:
2502 1104 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AleI CACnn'nnGTG
Cuts at: 1240 1240
Size: 4583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 377 377
Size: 4583
AlwNI CAG_nnn'CTG
Cuts at: 1489 1888 3455 1489
Size: 399 1567 2617
Fragments arranged by size:
2617 1567 399
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 126 235 484 619 853 1587 3936 4192 126
Size: 109 249 135 234 734 2349 256 517
Fragments arranged by size:
2349 734 517 256 249 234 135 109
AseI AT'TA_AT
Cuts at: 20 1375 20
Size: 1355 3228
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1603 1603
Size: 4583
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 1599 2013 2261 2296 1599
Size: 414 248 35 3886
Fragments arranged by size:
3886 414 248 35
BanII G_rGCy'C
Cuts at: 1553 1597 2627 1553
Size: 44 1030 3509
Fragments arranged by size:
3509 1030 44
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 194 1238 194
Size: 1044 3539
BciVI GTATCCnnnnn_n'
Cuts at: 2481 3242 4583 2481
Size: 761 1341 2481
Fragments arranged by size:
2481 1341 761
BclI T'GATC_A
Cuts at: 2103 2103
Size: 4583
BfrBI ATG'CAT
Cuts at: 1099 1099
Size: 4583
BglII A'GATC_T
Cuts at: 1395 2098 4135 1395
Size: 703 2037 1843
Fragments arranged by size:
2037 1843 703
BlpI GC'TnA_GC
Cuts at: 1695 1695
Size: 4583
Bme1580I G_kGCm'C
Cuts at: 2208 2301 3357 2208
Size: 93 1056 3434
Fragments arranged by size:
3434 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1795 1876 2211 4355 1441
Size: 354 81 335 2144 1669
Fragments arranged by size:
2144 1669 354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1570 1572 2950 1570
Size: 2 1378 3203
Fragments arranged by size:
3203 1378 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 1144 1521 154
Size: 990 377 3216
Fragments arranged by size:
3216 990 377
Bpu10I CC'TnA_GC
Cuts at: 1444 1444
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 1093 1516 1759 2638 3130 3428 183
Size: 910 423 243 879 492 298 1338
Fragments arranged by size:
1338 910 879 492 423 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 1936 1936
Size: 4583
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3245 3392 3803 1772
Size: 521 678 274 147 411 2552
Fragments arranged by size:
2552 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 615 1543 615
Size: 928 3655
BseYI C'CCAG_C
Cuts at: 2008 2766 3343 3839 2008
Size: 758 577 496 2752
Fragments arranged by size:
2752 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 866
Size: 4583
BsiEI CG_ry'CG
Cuts at: 104 2125 2171 3379 104
Size: 2021 46 1208 1308
Fragments arranged by size:
2021 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1553 1597 2375 2565 3357 1553
Size: 44 778 190 792 2779
Fragments arranged by size:
2779 792 778 190 44
BsmI GAATG_Cn'
Cuts at: 328 1101 328
Size: 773 3810
BsmBI CGTCTCn'nnnn_
Cuts at: 1120 1120
Size: 4583
Bsp1286I G_dGCh'C
Cuts at: 1553 1597 2208 2301 2375 2565 2627 3357 1553
Size: 44 611 93 74 190 62 730 2779
Fragments arranged by size:
2779 730 611 190 93 74 62 44
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 3759 3759
Size: 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 1633 1847 2149 2530 484
Size: 1149 214 302 381 2537
Fragments arranged by size:
2537 1149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2875 2929 4413 4578 50
Size: 2825 54 1484 165 55
Fragments arranged by size:
2825 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2495 2495
Size: 4583
BsrFI r'CCGG_y
Cuts at: 2581 2762 2971 2581
Size: 181 209 4193
Fragments arranged by size:
4193 209 181
BsrGI T'GTAC_A
Cuts at: 266 266
Size: 4583
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 1060 2944 1060
Size: 1884 2699
BstEII G'GTnAC_C
Cuts at: 117 871 117
Size: 754 3829
BstXI CCAn_nnnn'nTGG
Cuts at: 1358 1358
Size: 4583
BstYI r'GATC_y
Cuts at: 1 204 1395 1608 2075 2098 2433 2679 3691
Size: 203 1191 213 467 23 335 246 1012
Cuts at: 3691 3777 3789 4135 1
Size: 86 12 346 449
Fragments arranged by size:
1191 1012 467 449 346 335 246 213
203 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 658 2533 2714 2877 658
Size: 1875 181 163 2364
Fragments arranged by size:
2364 1875 181 163
BtsI GCAGTG_nn'
Cuts at: 849 1235 1826 849
Size: 386 591 3606
Fragments arranged by size:
3606 591 386
DraI TTT'AAA
Cuts at: 242 1582 242
Size: 1340 3243
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 2168 2342 2733 2760 4105 2168
Size: 174 391 27 1345 2646
Fragments arranged by size:
2646 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 335 1289 2606 2816 335
Size: 954 1317 210 2102
Fragments arranged by size:
2102 1317 954 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2791 3101 3247 4393 2791
Size: 310 146 1146 2981
Fragments arranged by size:
2981 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1551 1595 1551
Size: 44 4539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 359 568 800 1022 1144 1192 1250 1442
Size: 205 209 232 222 122 48 58 192
Cuts at: 1442 1521 2408 2840 3587 3855 154
Size: 79 887 432 747 268 882
Fragments arranged by size:
887 882 747 432 268 232 222 209
205 192 122 79 58 48
EcoO109I rG'GnC_Cy
Cuts at: 1722 1722
Size: 4583
EcoRI G'AATT_C
Cuts at: 126 619 1587 126
Size: 493 968 3122
Fragments arranged by size:
3122 968 493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 759 2602 759
Size: 1843 2740
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 1197 2017 2265 3287 4266 1197
Size: 820 248 1022 979 1514
Fragments arranged by size:
1514 1022 979 820 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 636 668 1249 1281 2425 2455 2457
Size: 32 423 32 581 32 1144 30 2
Cuts at: 2457 2487 181
Size: 30 2277
Fragments arranged by size:
2277 1144 581 423 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MfeI C'AATT_G
Cuts at: 638 638
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 890 3254 3438 890
Size: 2364 184 2035
Fragments arranged by size:
2364 2035 184
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 296 1240 2572 2699 296
Size: 944 1332 127 2180
Fragments arranged by size:
2180 1332 944 127
MspA1I CmG'CkG
Cuts at: 153 1694 2008 2368 3381 3626 153
Size: 1541 314 360 1013 245 1110
Fragments arranged by size:
1541 1110 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NsiI A_TGCA'T
Cuts at: 1101 1101
Size: 4583
NspI r_CATG'y
Cuts at: 364 1636 2136 3043 364
Size: 1272 500 907 1904
Fragments arranged by size:
1904 1272 907 500
PciI A'CATG_T
Cuts at: 3039 3039
Size: 4583
PflMI CCAn_nnn'nTGG
Cuts at: 928 928
Size: 4583
PfoI T'CCnGG_A
Cuts at: 1121 1121
Size: 4583
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 661 693 3722 3754 661
Size: 32 3029 32 1490
Fragments arranged by size:
3029 1490 32 32
PstI C_TGCA'G
Cuts at: 761 1630 2315 761
Size: 869 685 3029
Fragments arranged by size:
3029 869 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 153 2008 2368 153
Size: 1855 360 2368
Fragments arranged by size:
2368 1855 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1553 1597 1553
Size: 44 4539
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 1630 1630
Size: 4583
ScaI AGT'ACT
Cuts at: 4100 4100
Size: 4583
SfcI C'TryA_G
Cuts at: 33 757 1626 2311 3304 3495 33
Size: 724 869 685 993 191 1121
Fragments arranged by size:
1121 993 869 724 685 191
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 198 1072 1495 1738 2653 3145 3407 198
Size: 874 423 243 915 492 262 1374
Fragments arranged by size:
1374 915 874 492 423 262 243
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 1636 2136 1636
Size: 500 4083
StyI C'CwwG_G
Cuts at: 806 1717 2071 806
Size: 911 354 3318
Fragments arranged by size:
3318 911 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 680 941 2166 2299 2758 2967 680
Size: 261 1225 133 459 209 2296
Fragments arranged by size:
2296 1225 459 261 209 133
TatI w'GTAC_w
Cuts at: 266 4098 266
Size: 3832 751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4551
Tth111I GACn'n_nGTC
Cuts at: 193 2380 193
Size: 2187 2396
XbaI T'CTAG_A
Cuts at: 207 1614 207
Size: 1407 3176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 129 2078 129
Size: 1949 2634
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
XmnI GAAnn'nnTTC
Cuts at: 178 676 896 178
Size: 498 220 3865
Fragments arranged by size:
3865 498 220
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsiWI
BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI