Restriction map for
pF1KB7045
(Circular) (Six-base) MAPSORT of: pF1KB7045.seq Check: 210 from: 1 to: 4541
pF1KB7045 4541 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1058 1058
Size: 4541
AccI GT'mk_AC
Cuts at: 128 1579 128
Size: 1451 3090
Acc65I G'GTAC_C
Cuts at: 1459 1557 1459
Size: 98 4443
AclI AA'CG_TT
Cuts at: 4381 4381
Size: 4541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1227 2366 2798 3545 3813 1227
Size: 1139 432 747 268 1955
Fragments arranged by size:
1955 1139 747 432 268
AflIII A'CryG_T
Cuts at: 1893 2997 3974 1893
Size: 1104 977 2460
Fragments arranged by size:
2460 1104 977
AgeI A'CCGG_T
Cuts at: 1285 2929 1285
Size: 1644 2897
AleI CACnn'nnGTG
Cuts at: 211 1286 211
Size: 1075 3466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1161 1161
Size: 4541
AlwNI CAG_nnn'CTG
Cuts at: 1846 3413 1846
Size: 1567 2974
ApaI G_GGCC'C
Cuts at: 934 934
Size: 4541
ApaLI G'TGCA_C
Cuts at: 160 561 1111 3311 160
Size: 401 550 2200 1390
Fragments arranged by size:
2200 1390 550 401
ApoI r'AATT_y
Cuts at: 1545 3894 4150 1545
Size: 2349 256 1936
Fragments arranged by size:
2349 1936 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4541
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
AvaI C'yCGr_G
Cuts at: 1561 1561
Size: 4541
BamHI G'GATC_C
Cuts at: 1566 1566
Size: 4541
BanI G'GyrC_C
Cuts at: 418 879 1410 1459 1557 1971 2219 2254 418
Size: 461 531 49 98 414 248 35 2705
Fragments arranged by size:
2705 531 461 414 248 98 49 35
BanII G_rGCy'C
Cuts at: 934 1555 2585 934
Size: 621 1030 2890
Fragments arranged by size:
2890 1030 621
BbeI G_GCGC'C
Cuts at: 1975 2223 1975
Size: 248 4293
BciVI GTATCCnnnnn_n'
Cuts at: 2439 3200 4541 2439
Size: 761 1341 2439
Fragments arranged by size:
2439 1341 761
BclI T'GATC_A
Cuts at: 284 2061 284
Size: 1777 2764
BglI GCCn_nnn'nGGC
Cuts at: 1059 1059
Size: 4541
BglII A'GATC_T
Cuts at: 2056 4093 2056
Size: 2037 2504
BlpI GC'TnA_GC
Cuts at: 1002 1653 1002
Size: 651 3890
Bme1580I G_kGCm'C
Cuts at: 164 565 884 934 1115 1135 1398 2166 2259
Size: 401 319 50 181 20 263 768 93
Cuts at: 2259 3315 164
Size: 1056 1390
Fragments arranged by size:
1390 1056 768 401 319 263 181 93
50 20
BmgBI CAC'GTC
Cuts at: 209 209
Size: 4541
BmrI ACTGGGnnnn_n'
Cuts at: 133 247 929 1211 1753 1834 2169 4313 133
Size: 114 682 282 542 81 335 2144 361
Fragments arranged by size:
2144 682 542 361 335 282 114 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1519 2908 1519
Size: 1389 3152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 323
Size: 4541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1717 2596 3088 3386 1717
Size: 879 492 298 2872
Fragments arranged by size:
2872 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 638 766 809 4245 638
Size: 128 43 3436 934
Fragments arranged by size:
3436 934 128 43
BsaAI yAC'GTr
Cuts at: 1894 1894
Size: 4541
BsaBI GATnn'nnATC
Cuts at: 6 2055 4092 6
Size: 2049 2037 455
Fragments arranged by size:
2049 2037 455
BsaHI Gr'CG_yC
Cuts at: 1105 1972 2220 2922 4418 1105
Size: 867 248 702 1496 1228
Fragments arranged by size:
1496 1228 867 702 248
BsaWI w'CCGG_w
Cuts at: 949 1285 1730 2251 2929 3203 3350 3761 949
Size: 336 445 521 678 274 147 411 1729
Fragments arranged by size:
1729 678 521 445 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 608 611 1506 608
Size: 3 895 3643
Fragments arranged by size:
3643 895 3
BseYI C'CCAG_C
Cuts at: 334 486 578 940 1096 1134 1966 2724 3301
Size: 152 92 362 156 38 832 758 577
Cuts at: 3301 3797 334
Size: 496 1078
Fragments arranged by size:
1078 832 758 577 496 362 156 152
92 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 479 659 1468 1488 341
Size: 138 180 809 20 3394
Fragments arranged by size:
3394 809 180 138 20
BsiEI CG_ry'CG
Cuts at: 104 1152 2083 2129 3337 104
Size: 1048 931 46 1208 1308
Fragments arranged by size:
1308 1208 1048 931 46
BsiHKAI G_wGCw'C
Cuts at: 164 565 1115 1555 2333 2523 3315 164
Size: 401 550 440 778 190 792 1390
Fragments arranged by size:
1390 792 778 550 440 401 190
BsmBI CGTCTCn'nnnn_
Cuts at: 357 357
Size: 4541
Bsp1286I G_dGCh'C
Cuts at: 164 565 884 934 1115 1135 1398 1555 2166
Size: 401 319 50 181 20 263 157 611
Cuts at: 2166 2259 2333 2523 2585 3315 164
Size: 93 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 401 319 263 190 181
157 93 74 62 50 20
BspEI T'CCGG_A
Cuts at: 3761 3761
Size: 4541
BspHI T'CATG_A
Cuts at: 3717 3717
Size: 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1058 1591 1805 2107 2488 1058
Size: 533 214 302 381 3111
Fragments arranged by size:
3111 533 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1166 1383 1503 2833 2887 4371 4536 50
Size: 1116 217 120 1330 54 1484 165 55
Fragments arranged by size:
1484 1330 1116 217 165 120 55 54
BsrDI GCAATG_nn'
Cuts at: 2453 2453
Size: 4541
BsrFI r'CCGG_y
Cuts at: 1107 1151 1285 2539 2720 2929 1107
Size: 44 134 1254 181 209 2719
Fragments arranged by size:
2719 1254 209 181 134 44
BssSI C'ACGA_G
Cuts at: 1114 2812 3170 1114
Size: 1698 358 2485
Fragments arranged by size:
2485 1698 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1918 1918
Size: 4541
BstBI TT'CG_AA
Cuts at: 2902 2902
Size: 4541
BstEII G'GTnAC_C
Cuts at: 213 945 213
Size: 732 3809
BstXI CCAn_nnnn'nTGG
Cuts at: 537 537
Size: 4541
BstYI r'GATC_y
Cuts at: 1 321 1226 1361 1566 2033 2056 2391 2637
Size: 320 905 135 205 467 23 335 246
Cuts at: 2637 3649 3735 3747 4093 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 905 467 449 346 335 320 246
205 135 86 23 12
BtgI C'CryG_G
Cuts at: 749 749
Size: 4541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2491 2672 2835 2491
Size: 181 163 4197
Fragments arranged by size:
4197 181 163
BtsI GCAGTG_nn'
Cuts at: 1054 1257 1398 1784 1054
Size: 203 141 386 3811
Fragments arranged by size:
3811 386 203 141
DraI TTT'AAA
Cuts at: 1540 1540
Size: 4541
DrdI GACnn_nn'nnGTC
Cuts at: 2247 3105 2247
Size: 858 3683
EaeI y'GGCC_r
Cuts at: 269 539 1317 2126 2300 2691 2718 4063 269
Size: 270 778 809 174 391 27 1345 747
Fragments arranged by size:
1345 809 778 747 391 270 174 27
EagI C'GGCC_G
Cuts at: 2126 2126
Size: 4541
EarI CTCTTCn'nnn_
Cuts at: 304 702 769 2564 2774 304
Size: 398 67 1795 210 2071
Fragments arranged by size:
2071 1795 398 210 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 596 1508 2749 3059 3205 4351 596
Size: 912 1241 310 146 1146 786
Fragments arranged by size:
1241 1146 912 786 310 146
EcoICRI GAG'CTC
Cuts at: 1553 1553
Size: 4541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1227 2366 2798 3545 3813 323
Size: 904 1139 432 747 268 1051
Fragments arranged by size:
1139 1051 904 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 652 652
Size: 4541
EcoO109I rG'GnC_Cy
Cuts at: 195 244 547 657 930 931 979 1121 1680
Size: 49 303 110 273 1 48 142 559
Cuts at: 1680 195
Size: 3056
Fragments arranged by size:
3056 559 303 273 142 110 49 48
1
EcoRI G'AATT_C
Cuts at: 1545 1545
Size: 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4541
FspI TGC'GCA
Cuts at: 1237 2322 1237
Size: 1085 3456
HaeII r_GCGC'y
Cuts at: 1353 1975 2223 3245 4224 1353
Size: 622 248 1022 979 1670
Fragments arranged by size:
1670 1022 979 622 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 2383 2413 2415 2445 298
Size: 32 2053 30 2 30 2394
Fragments arranged by size:
2394 2053 32 30 30 2
HincII GTy'rAC
Cuts at: 1580 1580
Size: 4541
KasI G'GCGC_C
Cuts at: 1971 2219 1971
Size: 248 4293
KpnI G_GTAC'C
Cuts at: 1463 1561 1463
Size: 98 4443
MluI A'CGCG_T
Cuts at: 3974 3974
Size: 4541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 299 1204 3212 3396 299
Size: 905 2008 184 1444
Fragments arranged by size:
2008 1444 905 184
MscI TGG'CCA
Cuts at: 541 2302 541
Size: 1761 2780
MslI CAynn'nnrTG
Cuts at: 211 295 382 804 1286 2530 2657 211
Size: 84 87 422 482 1244 127 2095
Fragments arranged by size:
2095 1244 482 422 127 87 84
MspA1I CmG'CkG
Cuts at: 168 425 832 1652 1966 2326 3339 3584 168
Size: 257 407 820 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 820 407 360 314 257 245
NaeI GCC'GGC
Cuts at: 2722 2722
Size: 4541
NarI GG'CG_CC
Cuts at: 1972 2220 1972
Size: 248 4293
NgoMIV G'CCGG_C
Cuts at: 2720 2720
Size: 4541
NspI r_CATG'y
Cuts at: 1236 1594 2094 3001 1236
Size: 358 500 907 2776
Fragments arranged by size:
2776 907 500 358
PasI CC'CwG_GG
Cuts at: 552 924 552
Size: 372 4169
PciI A'CATG_T
Cuts at: 2997 2997
Size: 4541
PfoI T'CCnGG_A
Cuts at: 786 786
Size: 4541
PmeI GTTT'AAAC
Cuts at: 1540 1540
Size: 4541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4509
PpuMI rG'GwC_Cy
Cuts at: 195 547 657 979 195
Size: 352 110 322 3757
Fragments arranged by size:
3757 352 322 110
PshAI GACnn'nnGTC
Cuts at: 758 758
Size: 4541
PspOMI G'GGCC_C
Cuts at: 930 930
Size: 4541
PstI C_TGCA'G
Cuts at: 1588 2273 1588
Size: 685 3856
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4541
PvuII CAG'CTG
Cuts at: 168 425 832 1966 2326 168
Size: 257 407 1134 360 2383
Fragments arranged by size:
2383 1134 407 360 257
RsrII CG'GwC_CG
Cuts at: 2736 2736
Size: 4541
SacI G_AGCT'C
Cuts at: 1555 1555
Size: 4541
SalI G'TCGA_C
Cuts at: 1578 1578
Size: 4541
SanDI GG'GwC_CC
Cuts at: 979 979
Size: 4541
SapI GCTCTTCn'nnn_
Cuts at: 2564 2774 2564
Size: 210 4331
SbfI CC_TGCA'GG
Cuts at: 1588 1588
Size: 4541
ScaI AGT'ACT
Cuts at: 4058 4058
Size: 4541
SfcI C'TryA_G
Cuts at: 33 1424 1584 2269 3262 3453 33
Size: 1391 160 685 993 191 1121
Fragments arranged by size:
1391 1121 993 685 191 160
SfoI GGC'GCC
Cuts at: 1973 2221 1973
Size: 248 4293
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4541
SgrAI Cr'CCGG_yG
Cuts at: 1107 1285 1107
Size: 178 4363
SmaI CCC'GGG
Cuts at: 1563 1563
Size: 4541
SmlI C'TyrA_G
Cuts at: 1696 2611 3103 3365 1696
Size: 915 492 262 2872
Fragments arranged by size:
2872 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4541
SphI G_CATG'C
Cuts at: 1236 1594 2094 1236
Size: 358 500 3683
Fragments arranged by size:
3683 500 358
SspI AAT'ATT
Cuts at: 450 450
Size: 4541
StuI AGG'CCT
Cuts at: 1074 1074
Size: 4541
StyI C'CwwG_G
Cuts at: 119 1675 2029 119
Size: 1556 354 2631
Fragments arranged by size:
2631 1556 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 544 1282 1294 2124 2257 2716 2925 544
Size: 738 12 830 133 459 209 2160
Fragments arranged by size:
2160 830 738 459 209 133 12
TatI w'GTAC_w
Cuts at: 599 4056 599
Size: 3457 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1172 1204 1377 1409 4058 4090 1172
Size: 32 173 32 2649 32 1623
Fragments arranged by size:
2649 1623 173 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2338 2338
Size: 4541
XbaI T'CTAG_A
Cuts at: 1572 1572
Size: 4541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 549 2036 234
Size: 315 1487 2739
Fragments arranged by size:
2739 1487 315
XmaI C'CCGG_G
Cuts at: 1561 1561
Size: 4541
XmnI GAAnn'nnTTC
Cuts at: 1297 1297
Size: 4541
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SacII SexAI SfiI SnaBI SrfI SwaI
XhoI ZraI