Restriction map for
pF1KB7041
(Circular) (Six-base) MAPSORT of: pF1KB7041.seq Check: 5077 from: 1 to: 4829
pF1KB7041 4829 bp
With 182 enzymes: SgfI *
February 7, 2012 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 494 1780 494
Size: 1286 3543
AccI GT'mk_AC
Cuts at: 1406 1867 1406
Size: 461 4368
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2654 3086 3833 4101 1258
Size: 1396 432 747 268 1986
Fragments arranged by size:
1986 1396 747 432 268
AfeI AGC'GCT
Cuts at: 838 1714 838
Size: 876 3953
AflIII A'CryG_T
Cuts at: 2181 3285 4262 2181
Size: 1104 977 2748
Fragments arranged by size:
2748 1104 977
AgeI A'CCGG_T
Cuts at: 686 3217 686
Size: 2531 2298
AlwNI CAG_nnn'CTG
Cuts at: 668 2134 3701 668
Size: 1466 1567 1796
Fragments arranged by size:
1796 1567 1466
ApaI G_GGCC'C
Cuts at: 229 800 229
Size: 571 4258
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 1833 4182 4438 1833
Size: 2349 256 2224
Fragments arranged by size:
2349 2224 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 970 1458 1540 1849 970
Size: 488 82 309 3950
Fragments arranged by size:
3950 488 309 82
AvrII C'CTAG_G
Cuts at: 1737 1737
Size: 4829
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 700 733 700
Size: 33 4796
BamHI G'GATC_C
Cuts at: 1854 1854
Size: 4829
BanI G'GyrC_C
Cuts at: 1048 1382 1765 1845 2259 2507 2542 1048
Size: 334 383 80 414 248 35 3335
Fragments arranged by size:
3335 414 383 334 248 80 35
BanII G_rGCy'C
Cuts at: 175 229 310 800 821 1843 2873 175
Size: 54 81 490 21 1022 1030 2131
Fragments arranged by size:
2131 1030 1022 490 81 54 21
BbeI G_GCGC'C
Cuts at: 1052 1386 1769 2263 2511 1052
Size: 334 383 494 248 3370
Fragments arranged by size:
3370 494 383 334 248
BbsI GAAGACnn'nnnn_
Cuts at: 402 867 1167 1248 402
Size: 465 300 81 3983
Fragments arranged by size:
3983 465 300 81
BbvCI CC'TCA_GC
Cuts at: 661 661
Size: 4829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1617 1651 1617
Size: 34 4795
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3488 4829 2727
Size: 761 1341 2727
Fragments arranged by size:
2727 1341 761
BclI T'GATC_A
Cuts at: 2349 2349
Size: 4829
BglI GCCn_nnn'nGGC
Cuts at: 757 1057 757
Size: 300 4529
BglII A'GATC_T
Cuts at: 2344 4381 2344
Size: 2037 2792
BlpI GC'TnA_GC
Cuts at: 541 1941 541
Size: 1400 3429
Bme1580I G_kGCm'C
Cuts at: 229 800 827 2454 2547 3603 229
Size: 571 27 1627 93 1056 1455
Fragments arranged by size:
1627 1455 1056 571 93 27
BmgBI CAC'GTC
Cuts at: 348 636 348
Size: 288 4541
BmrI ACTGGGnnnn_n'
Cuts at: 2041 2122 2457 4601 2041
Size: 81 335 2144 2269
Fragments arranged by size:
2269 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 4829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1635 159
Size: 1476 3353
Bpu10I CC'TnA_GC
Cuts at: 127 661 127
Size: 534 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1105 2005 2884 3376 3674 697
Size: 408 900 879 492 298 1852
Fragments arranged by size:
1852 900 879 492 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 1614 1807 4533 1614
Size: 193 2726 1910
Fragments arranged by size:
2726 1910 193
BsaAI yAC'GTr
Cuts at: 693 942 2182 693
Size: 249 1240 3340
Fragments arranged by size:
3340 1240 249
BsaBI GATnn'nnATC
Cuts at: 6 2343 4380 6
Size: 2337 2037 455
Fragments arranged by size:
2337 2037 455
BsaHI Gr'CG_yC
Cuts at: 407 980 1049 1253 1383 1742 1766 2260 2508
Size: 573 69 204 130 359 24 494 248
Cuts at: 2508 3210 4706 407
Size: 702 1496 530
Fragments arranged by size:
1496 702 573 530 494 359 248 204
130 69 24
BsaWI w'CCGG_w
Cuts at: 302 686 1554 1616 2018 2539 3217 3491 3638
Size: 384 868 62 402 521 678 274 147
Cuts at: 3638 4049 302
Size: 411 1082
Fragments arranged by size:
1082 868 678 521 411 402 384 274
147 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 292 322 1717 1747 292
Size: 30 1395 30 3374
Fragments arranged by size:
3374 1395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 870 871 870
Size: 1 4828
BseYI C'CCAG_C
Cuts at: 720 804 1579 1605 1631 1709 2254 3012 3589
Size: 84 775 26 26 78 545 758 577
Cuts at: 3589 4085 720
Size: 496 1464
Fragments arranged by size:
1464 775 758 577 545 496 84 78
26 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 654 630
Size: 24 4805
BsiEI CG_ry'CG
Cuts at: 104 365 749 1559 2371 2417 3625 104
Size: 261 384 810 812 46 1208 1308
Fragments arranged by size:
1308 1208 812 810 384 261 46
BsiHKAI G_wGCw'C
Cuts at: 310 892 1184 1843 2621 2811 3603 310
Size: 582 292 659 778 190 792 1536
Fragments arranged by size:
1536 792 778 659 582 292 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1357 1357
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 175 229 310 800 821 827 892 1184 1843
Size: 54 81 490 21 6 65 292 659
Cuts at: 1843 2454 2547 2621 2811 2873 3603 175
Size: 611 93 74 190 62 730 1401
Fragments arranged by size:
1401 730 659 611 490 292 190 93
81 74 65 62 54 21 6
BspEI T'CCGG_A
Cuts at: 1616 4049 1616
Size: 2433 2396
BspHI T'CATG_A
Cuts at: 728 4005 728
Size: 3277 1552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 433 458 494 542 908 1780 1879 2093 2395
Size: 25 36 48 366 872 99 214 302
Cuts at: 2395 2776 433
Size: 381 2486
Fragments arranged by size:
2486 872 381 366 302 214 99 48
36 25
BsrBI CCG'CTC
Cuts at: 50 1215 1305 3121 3175 4659 4824 50
Size: 1165 90 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 1165 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 2741 2741
Size: 4829
BsrFI r'CCGG_y
Cuts at: 686 1379 2827 3008 3217 686
Size: 693 1448 181 209 2298
Fragments arranged by size:
2298 1448 693 209 181
BssHII G'CGCG_C
Cuts at: 386 949 1338 1638 1682 1689 386
Size: 563 389 300 44 7 3526
Fragments arranged by size:
3526 563 389 300 44 7
BssSI C'ACGA_G
Cuts at: 1750 3100 3458 1750
Size: 1350 358 3121
Fragments arranged by size:
3121 1350 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1068 2206 1068
Size: 1138 3691
BstBI TT'CG_AA
Cuts at: 3190 3190
Size: 4829
BstEII G'GTnAC_C
Cuts at: 445 445
Size: 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 681 1155 1167 681
Size: 474 12 4343
Fragments arranged by size:
4343 474 12
BstYI r'GATC_y
Cuts at: 1 1667 1854 2321 2344 2679 2925 3937 4023
Size: 1666 187 467 23 335 246 1012 86
Cuts at: 4023 4035 4381 1
Size: 12 346 449
Fragments arranged by size:
1666 1012 467 449 346 335 246 187
86 23 12
BtgI C'CryG_G
Cuts at: 311 437 524 572 629 680 905 1043 311
Size: 126 87 48 57 51 225 138 4097
Fragments arranged by size:
4097 225 138 126 87 57 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2779 2960 3123 2779
Size: 181 163 4485
Fragments arranged by size:
4485 181 163
BtsI GCAGTG_nn'
Cuts at: 2072 2072
Size: 4829
DraI TTT'AAA
Cuts at: 1828 1828
Size: 4829
DraIII CAC_nnn'GTG
Cuts at: 1755 1755
Size: 4829
DrdI GACnn_nn'nnGTC
Cuts at: 2535 3393 2535
Size: 858 3971
EaeI y'GGCC_r
Cuts at: 362 434 521 527 566 600 746 1040 1145
Size: 72 87 6 39 34 146 294 105
Cuts at: 1145 1157 1410 1757 1790 2414 2588 2979 3006
Size: 12 253 347 33 624 174 391 27
Cuts at: 3006 4351 362
Size: 1345 840
Fragments arranged by size:
1345 840 624 391 347 294 253 174
146 105 87 72 39 34 33 27
12 6
EagI C'GGCC_G
Cuts at: 362 746 2414 362
Size: 384 1668 2777
Fragments arranged by size:
2777 1668 384
EarI CTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3037 3347 3493 4639 3037
Size: 310 146 1146 3227
Fragments arranged by size:
3227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 308 1841 308
Size: 1533 3296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 1258 1635 2654 3086 3833 4101 159
Size: 1099 377 1019 432 747 268 887
Fragments arranged by size:
1099 1019 887 747 432 377 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1444 1444
Size: 4829
EcoO109I rG'GnC_Cy
Cuts at: 145 225 796 797 1968 145
Size: 80 571 1 1171 3006
Fragments arranged by size:
3006 1171 571 80 1
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1284 1359 2848 1284
Size: 75 1489 3265
Fragments arranged by size:
3265 1489 75
FspI TGC'GCA
Cuts at: 1021 1072 2610 1021
Size: 51 1538 3240
Fragments arranged by size:
3240 1538 51
HaeII r_GCGC'y
Cuts at: 548 840 1052 1079 1386 1489 1586 1716 1769
Size: 292 212 27 307 103 97 130 53
Cuts at: 1769 2263 2511 3533 4512 548
Size: 494 248 1022 979 865
Fragments arranged by size:
1022 979 865 494 307 292 248 212
130 103 97 53 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1536 1568 1717 1749 2671 2701 2703 2733 1536
Size: 32 149 32 922 30 2 30 3632
Fragments arranged by size:
3632 922 149 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1868 1868
Size: 4829
KasI G'GCGC_C
Cuts at: 1048 1382 1765 2259 2507 1048
Size: 334 383 494 248 3370
Fragments arranged by size:
3370 494 383 334 248
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3500 3684 3500
Size: 184 4645
MscI TGG'CCA
Cuts at: 1159 2590 1159
Size: 1431 3398
MslI CAynn'nnrTG
Cuts at: 430 679 1165 2818 2945 430
Size: 249 486 1653 127 2314
Fragments arranged by size:
2314 1653 486 249 127
MspA1I CmG'CkG
Cuts at: 290 313 1236 1292 1436 1491 1635 1940 2254
Size: 23 923 56 144 55 144 305 314
Cuts at: 2254 2614 3627 3872 290
Size: 360 1013 245 1247
Fragments arranged by size:
1247 1013 923 360 314 305 245 144
144 56 55 23
NaeI GCC'GGC
Cuts at: 1381 3010 1381
Size: 1629 3200
NarI GG'CG_CC
Cuts at: 1049 1383 1766 2260 2508 1049
Size: 334 383 494 248 3370
Fragments arranged by size:
3370 494 383 334 248
NcoI C'CATG_G
Cuts at: 572 905 572
Size: 333 4496
NgoMIV G'CCGG_C
Cuts at: 1379 3008 1379
Size: 1629 3200
NotI GC'GGCC_GC
Cuts at: 746 746
Size: 4829
NspI r_CATG'y
Cuts at: 1071 1882 2382 3289 1071
Size: 811 500 907 2611
Fragments arranged by size:
2611 907 811 500
PciI A'CATG_T
Cuts at: 3285 3285
Size: 4829
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PpuMI rG'GwC_Cy
Cuts at: 145 145
Size: 4829
PspOMI G'GGCC_C
Cuts at: 225 796 225
Size: 571 4258
PspXI vC'TCGA_Gb
Cuts at: 970 970
Size: 4829
PstI C_TGCA'G
Cuts at: 491 1876 2561 491
Size: 1385 685 2759
Fragments arranged by size:
2759 1385 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4829
PvuII CAG'CTG
Cuts at: 1236 1292 1635 2254 2614 1236
Size: 56 343 619 360 3451
Fragments arranged by size:
3451 619 360 343 56
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 310 1843 310
Size: 1533 3296
SacII CC_GC'GG
Cuts at: 314 314
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SanDI GG'GwC_CC
Cuts at: 145 145
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 4346 4346
Size: 4829
SfcI C'TryA_G
Cuts at: 33 487 1797 1872 2557 3550 3741 33
Size: 454 1310 75 685 993 191 1121
Fragments arranged by size:
1310 1121 993 685 454 191 75
SfoI GGC'GCC
Cuts at: 1050 1384 1767 2261 2509 1050
Size: 334 383 494 248 3370
Fragments arranged by size:
3370 494 383 334 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SgrAI Cr'CCGG_yG
Cuts at: 1379 1379
Size: 4829
SmaI CCC'GGG
Cuts at: 1851 1851
Size: 4829
SmlI C'TyrA_G
Cuts at: 712 970 1120 1984 2899 3391 3653 712
Size: 258 150 864 915 492 262 1888
Fragments arranged by size:
1888 915 864 492 262 258 150
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1071 1882 2382 1071
Size: 811 500 3518
Fragments arranged by size:
3518 811 500
StuI AGG'CCT
Cuts at: 1003 1780 1003
Size: 777 4052
StyI C'CwwG_G
Cuts at: 572 905 1079 1737 1963 2317 572
Size: 333 174 658 226 354 3084
Fragments arranged by size:
3084 658 354 333 226 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 748 1159 1593 1831 2412 2545 3004 3213 748
Size: 411 434 238 581 133 459 209 2364
Fragments arranged by size:
2364 581 459 434 411 238 209 133
TatI w'GTAC_w
Cuts at: 926 4344 926
Size: 3418 1411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 625 657 4346 4378 365
Size: 32 228 32 3689 32 816
Fragments arranged by size:
3689 816 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2626 2626
Size: 4829
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 2324 579
Size: 1745 3084
XhoI C'TCGA_G
Cuts at: 970 970
Size: 4829
XmaI C'CCGG_G
Cuts at: 1849 1849
Size: 4829
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BfrBI
BmtI BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PsrI SexAI SfiI SnaBI SrfI SspI SwaI
XmnI ZraI