Restriction map for
pF1KB7038
(Circular) (Six-base) MAPSORT of: pF1KB7038.seq Check: 1310 from: 1 to: 4484
pF1KB7038 4484 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1160 1160
Size: 4484
AccI GT'mk_AC
Cuts at: 1522 1522
Size: 4484
Acc65I G'GTAC_C
Cuts at: 1500 1500
Size: 4484
AclI AA'CG_TT
Cuts at: 4324 4324
Size: 4484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 916 1093 1219 2309 2741 3488 3756 781
Size: 135 177 126 1090 432 747 268 1509
Fragments arranged by size:
1509 1090 747 432 268 177 135 126
AfeI AGC'GCT
Cuts at: 712 868 712
Size: 156 4328
AflIII A'CryG_T
Cuts at: 230 299 830 1836 2940 3917 230
Size: 69 531 1006 1104 977 797
Fragments arranged by size:
1104 1006 977 797 531 69
AgeI A'CCGG_T
Cuts at: 2872 2872
Size: 4484
AlwNI CAG_nnn'CTG
Cuts at: 1789 3356 1789
Size: 1567 2917
ApaI G_GGCC'C
Cuts at: 1162 1384 1162
Size: 222 4262
ApaLI G'TGCA_C
Cuts at: 3254 3254
Size: 4484
ApoI r'AATT_y
Cuts at: 1488 3837 4093 1488
Size: 2349 256 1879
Fragments arranged by size:
2349 1879 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4484
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
AvaI C'yCGr_G
Cuts at: 172 270 445 822 967 1023 1123 1173 1504
Size: 98 175 377 145 56 100 50 331
Cuts at: 1504 172
Size: 3152
Fragments arranged by size:
3152 377 331 175 145 100 98 56
50
BamHI G'GATC_C
Cuts at: 1509 1509
Size: 4484
BanI G'GyrC_C
Cuts at: 361 765 910 1006 1113 1500 1914 2162 2197
Size: 404 145 96 107 387 414 248 35
Cuts at: 2197 361
Size: 2648
Fragments arranged by size:
2648 414 404 387 248 145 107 96
35
BanII G_rGCy'C
Cuts at: 173 292 331 442 511 634 799 948 1102
Size: 119 39 111 69 123 165 149 154
Cuts at: 1102 1162 1384 1498 2528 173
Size: 60 222 114 1030 2129
Fragments arranged by size:
2129 1030 222 165 154 149 123 119
114 111 69 60 39
BbeI G_GCGC'C
Cuts at: 365 914 1117 1918 2166 365
Size: 549 203 801 248 2683
Fragments arranged by size:
2683 801 549 248 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 816 850 90
Size: 34 692 34 3724
Fragments arranged by size:
3724 692 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2382 3143 4484 2382
Size: 761 1341 2382
Fragments arranged by size:
2382 1341 761
BclI T'GATC_A
Cuts at: 2004 2004
Size: 4484
BglI GCCn_nnn'nGGC
Cuts at: 394 403 958 985 394
Size: 9 555 27 3893
Fragments arranged by size:
3893 555 27 9
BglII A'GATC_T
Cuts at: 659 1999 4036 659
Size: 1340 2037 1107
Fragments arranged by size:
2037 1340 1107
BlpI GC'TnA_GC
Cuts at: 652 1096 1596 652
Size: 444 500 3540
Fragments arranged by size:
3540 500 444
Bme1580I G_kGCm'C
Cuts at: 137 770 1009 1162 1384 2109 2202 3258 137
Size: 633 239 153 222 725 93 1056 1363
Fragments arranged by size:
1363 1056 725 633 239 222 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 1696 1777 2112 4256 1696
Size: 81 335 2144 1924
Fragments arranged by size:
2144 1924 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 2851 198
Size: 2653 1831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 171
Size: 4484
Bpu10I CC'TnA_GC
Cuts at: 436 532 436
Size: 96 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 1297 1660 2539 3031 3329 444
Size: 853 363 879 492 298 1599
Fragments arranged by size:
1599 879 853 492 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 4188 4188
Size: 4484
BsaAI yAC'GTr
Cuts at: 233 312 1837 233
Size: 79 1525 2880
Fragments arranged by size:
2880 1525 79
BsaBI GATnn'nnATC
Cuts at: 6 1998 4035 6
Size: 1992 2037 455
Fragments arranged by size:
2037 1992 455
BsaHI Gr'CG_yC
Cuts at: 362 911 1081 1114 1409 1915 2163 2865 4361
Size: 549 170 33 295 506 248 702 1496
Cuts at: 4361 362
Size: 485
Fragments arranged by size:
1496 702 549 506 485 295 248 170
33
BsaWI w'CCGG_w
Cuts at: 1673 2194 2872 3146 3293 3704 1673
Size: 521 678 274 147 411 2453
Fragments arranged by size:
2453 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 484 514 724 754 1219 1249 484
Size: 30 210 30 465 30 3719
Fragments arranged by size:
3719 465 210 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 174 1464 174
Size: 1290 3194
BseYI C'CCAG_C
Cuts at: 111 430 1155 1909 2667 3244 3740 111
Size: 319 725 754 758 577 496 855
Fragments arranged by size:
855 758 754 725 577 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 796
Size: 4484
BsiEI CG_ry'CG
Cuts at: 104 254 389 398 461 938 2026 2072 3280
Size: 150 135 9 63 477 1088 46 1208
Cuts at: 3280 104
Size: 1308
Fragments arranged by size:
1308 1208 1088 477 150 135 63 46
9
BsiHKAI G_wGCw'C
Cuts at: 1049 1498 2276 2466 3258 1049
Size: 449 778 190 792 2275
Fragments arranged by size:
2275 792 778 449 190
BsiWI C'GTAC_G
Cuts at: 308 308
Size: 4484
Bsp1286I G_dGCh'C
Cuts at: 137 173 292 331 442 511 634 770 799
Size: 36 119 39 111 69 123 136 29
Cuts at: 799 948 1009 1049 1102 1162 1384 1498 2109
Size: 149 61 40 53 60 222 114 611
Cuts at: 2109 2202 2276 2466 2528 3258 137
Size: 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 222 190 149 136 123
119 114 111 93 74 69 62 61
60 53 40 39 36 29
BspEI T'CCGG_A
Cuts at: 3704 3704
Size: 4484
BspHI T'CATG_A
Cuts at: 3660 3660
Size: 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 860 1160 1534 1748 2050 2431 860
Size: 300 374 214 302 381 2913
Fragments arranged by size:
2913 381 374 302 300 214
BsrBI CCG'CTC
Cuts at: 50 118 444 773 2776 2830 4314 4479 50
Size: 68 326 329 2003 54 1484 165 55
Fragments arranged by size:
2003 1484 329 326 165 68 55 54
BsrDI GCAATG_nn'
Cuts at: 2396 2396
Size: 4484
BsrFI r'CCGG_y
Cuts at: 205 397 695 916 984 1083 1110 2482 2663
Size: 192 298 221 68 99 27 1372 181
Cuts at: 2663 2872 205
Size: 209 1817
Fragments arranged by size:
1817 1372 298 221 209 192 181 99
68 27
BssSI C'ACGA_G
Cuts at: 136 2755 3113 136
Size: 2619 358 1507
Fragments arranged by size:
2619 1507 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1861 1861
Size: 4484
BstBI TT'CG_AA
Cuts at: 2845 2845
Size: 4484
BstYI r'GATC_y
Cuts at: 1 659 1509 1976 1999 2334 2580 3592 3678
Size: 658 850 467 23 335 246 1012 86
Cuts at: 3678 3690 4036 1
Size: 12 346 449
Fragments arranged by size:
1012 850 658 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1187 1187
Size: 4484
BtgI C'CryG_G
Cuts at: 257 365 467 614 1142 1376 257
Size: 108 102 147 528 234 3365
Fragments arranged by size:
3365 528 234 147 108 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2434 2615 2778 2434
Size: 181 163 4140
Fragments arranged by size:
4140 181 163
BtsI GCAGTG_nn'
Cuts at: 1727 1727
Size: 4484
DraI TTT'AAA
Cuts at: 1483 1483
Size: 4484
DrdI GACnn_nn'nnGTC
Cuts at: 2190 3048 2190
Size: 858 3626
EaeI y'GGCC_r
Cuts at: 251 386 395 458 617 935 1373 1421 2069
Size: 135 9 63 159 318 438 48 648
Cuts at: 2069 2243 2634 2661 4006 251
Size: 174 391 27 1345 729
Fragments arranged by size:
1345 729 648 438 391 318 174 159
135 63 48 27 9
EagI C'GGCC_G
Cuts at: 251 386 395 458 935 2069 251
Size: 135 9 63 477 1134 2666
Fragments arranged by size:
2666 1134 477 135 63 9
EarI CTCTTCn'nnn_
Cuts at: 688 2507 2717 688
Size: 1819 210 2455
Fragments arranged by size:
2455 1819 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 718 2692 3002 3148 4294 718
Size: 1974 310 146 1146 908
Fragments arranged by size:
1974 1146 908 310 146
EcoICRI GAG'CTC
Cuts at: 1496 1496
Size: 4484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 781 916 1093 1219 2309 2741 3488 3756
Size: 610 135 177 126 1090 432 747 268
Cuts at: 3756 171
Size: 899
Fragments arranged by size:
1090 899 747 610 432 268 177 135
126
EcoO109I rG'GnC_Cy
Cuts at: 1059 1623 1059
Size: 564 3920
EcoRI G'AATT_C
Cuts at: 1488 1488
Size: 4484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 816 2503 816
Size: 1687 2797
FseI GG_CCGG'CC
Cuts at: 988 988
Size: 4484
FspI TGC'GCA
Cuts at: 2265 2265
Size: 4484
HaeII r_GCGC'y
Cuts at: 320 365 714 870 895 914 1031 1096 1117
Size: 45 349 156 25 19 117 65 21
Cuts at: 1117 1918 2166 3188 4167 320
Size: 801 248 1022 979 637
Fragments arranged by size:
1022 979 801 637 349 248 156 117
65 45 25 21 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2326 2356 2358 2388 2326
Size: 30 2 30 4422
Fragments arranged by size:
4422 30 30 2
HincII GTy'rAC
Cuts at: 1523 1523
Size: 4484
KasI G'GCGC_C
Cuts at: 361 910 1113 1914 2162 361
Size: 549 203 801 248 2683
Fragments arranged by size:
2683 801 549 248 203
KpnI G_GTAC'C
Cuts at: 1504 1504
Size: 4484
MluI A'CGCG_T
Cuts at: 3917 3917
Size: 4484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 3155 3339 170
Size: 2985 184 1315
Fragments arranged by size:
2985 1315 184
MscI TGG'CCA
Cuts at: 619 1375 2245 619
Size: 756 870 2858
Fragments arranged by size:
2858 870 756
MslI CAynn'nnrTG
Cuts at: 235 241 298 613 2473 2600 235
Size: 6 57 315 1860 127 2119
Fragments arranged by size:
2119 1860 315 127 57 6
MspA1I CmG'CkG
Cuts at: 266 888 923 1144 1595 1909 2269 3282 3527
Size: 622 35 221 451 314 360 1013 245
Cuts at: 3527 266
Size: 1223
Fragments arranged by size:
1223 1013 622 451 360 314 245 221
35
NaeI GCC'GGC
Cuts at: 207 399 918 986 1085 1112 2665 207
Size: 192 519 68 99 27 1553 2026
Fragments arranged by size:
2026 1553 519 192 99 68 27
NarI GG'CG_CC
Cuts at: 362 911 1114 1915 2163 362
Size: 549 203 801 248 2683
Fragments arranged by size:
2683 801 549 248 203
NcoI C'CATG_G
Cuts at: 257 365 467 614 1376 257
Size: 108 102 147 762 3365
Fragments arranged by size:
3365 762 147 108 102
NgoMIV G'CCGG_C
Cuts at: 205 397 916 984 1083 1110 2663 205
Size: 192 519 68 99 27 1553 2026
Fragments arranged by size:
2026 1553 519 192 99 68 27
NotI GC'GGCC_GC
Cuts at: 251 935 251
Size: 684 3800
NspI r_CATG'y
Cuts at: 246 303 834 1537 2037 2944 246
Size: 57 531 703 500 907 1786
Fragments arranged by size:
1786 907 703 531 500 57
PciI A'CATG_T
Cuts at: 299 830 2940 299
Size: 531 2110 1843
Fragments arranged by size:
2110 1843 531
PflMI CCAn_nnn'nTGG
Cuts at: 1155 1305 1155
Size: 150 4334
PmeI GTTT'AAAC
Cuts at: 1483 1483
Size: 4484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4452
PspOMI G'GGCC_C
Cuts at: 1158 1380 1158
Size: 222 4262
PspXI vC'TCGA_Gb
Cuts at: 1023 1023
Size: 4484
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 832 864 832
Size: 32 4452
PstI C_TGCA'G
Cuts at: 1531 2216 1531
Size: 685 3799
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4484
PvuII CAG'CTG
Cuts at: 888 1909 2269 888
Size: 1021 360 3103
Fragments arranged by size:
3103 1021 360
RsrII CG'GwC_CG
Cuts at: 2679 2679
Size: 4484
SacI G_AGCT'C
Cuts at: 1498 1498
Size: 4484
SacII CC_GC'GG
Cuts at: 1145 1145
Size: 4484
SalI G'TCGA_C
Cuts at: 1521 1521
Size: 4484
SapI GCTCTTCn'nnn_
Cuts at: 2507 2717 2507
Size: 210 4274
SbfI CC_TGCA'GG
Cuts at: 1531 1531
Size: 4484
ScaI AGT'ACT
Cuts at: 4001 4001
Size: 4484
SfcI C'TryA_G
Cuts at: 33 558 1527 2212 3205 3396 33
Size: 525 969 685 993 191 1121
Fragments arranged by size:
1121 993 969 685 525 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 394 394
Size: 4484
SfoI GGC'GCC
Cuts at: 363 912 1115 1916 2164 363
Size: 549 203 801 248 2683
Fragments arranged by size:
2683 801 549 248 203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
SmaI CCC'GGG
Cuts at: 1125 1175 1506 1125
Size: 50 331 4103
Fragments arranged by size:
4103 331 50
SmlI C'TyrA_G
Cuts at: 423 1023 1312 1639 2554 3046 3308 423
Size: 600 289 327 915 492 262 1599
Fragments arranged by size:
1599 915 600 492 327 289 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4484
SphI G_CATG'C
Cuts at: 1537 2037 1537
Size: 500 3984
StuI AGG'CCT
Cuts at: 952 1318 952
Size: 366 4118
StyI C'CwwG_G
Cuts at: 257 365 467 614 1376 1618 1972 257
Size: 108 102 147 762 242 354 2769
Fragments arranged by size:
2769 762 354 242 147 108 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2067 2200 2659 2868 2067
Size: 133 459 209 3683
Fragments arranged by size:
3683 459 209 133
TatI w'GTAC_w
Cuts at: 1451 3999 1451
Size: 2548 1936
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 724 756 1219 1251 4001 4033 127
Size: 32 565 32 463 32 2750 32 578
Fragments arranged by size:
2750 578 565 463 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2281 2281
Size: 4484
XbaI T'CTAG_A
Cuts at: 1515 1515
Size: 4484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 474 1979 372
Size: 102 1505 2877
Fragments arranged by size:
2877 1505 102
XhoI C'TCGA_G
Cuts at: 1023 1023
Size: 4484
XmaI C'CCGG_G
Cuts at: 1123 1173 1504 1123
Size: 50 331 4103
Fragments arranged by size:
4103 331 50
XmnI GAAnn'nnTTC
Cuts at: 868 868
Size: 4484
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
ZraI