Restriction map for
pF1KB7035
(Circular) (Six-base) MAPSORT of: pF1KB7035.seq Check: 1043 from: 1 to: 4391
pF1KB7035 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 740 1429 740
Size: 689 3702
Acc65I G'GTAC_C
Cuts at: 339 1407 339
Size: 1068 3323
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1028 2216 2648 3395 3663 1028
Size: 1188 432 747 268 1756
Fragments arranged by size:
1756 1188 747 432 268
AflIII A'CryG_T
Cuts at: 1743 2847 3824 1743
Size: 1104 977 2310
Fragments arranged by size:
2310 1104 977
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AhdI GACnn_n'nnGTC
Cuts at: 213 325 213
Size: 112 4279
AlwNI CAG_nnn'CTG
Cuts at: 1696 3263 1696
Size: 1567 2824
ApaLI G'TGCA_C
Cuts at: 3161 3161
Size: 4391
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1170 1411 1170
Size: 241 4150
BamHI G'GATC_C
Cuts at: 181 1416 181
Size: 1235 3156
BanI G'GyrC_C
Cuts at: 339 859 1407 1821 2069 2104 339
Size: 520 548 414 248 35 2626
Fragments arranged by size:
2626 548 520 414 248 35
BanII G_rGCy'C
Cuts at: 817 1405 2435 817
Size: 588 1030 2773
Fragments arranged by size:
2773 1030 588
BbeI G_GCGC'C
Cuts at: 863 1825 2073 863
Size: 962 248 3181
Fragments arranged by size:
3181 962 248
BbsI GAAGACnn'nnnn_
Cuts at: 514 759 1016 514
Size: 245 257 3889
Fragments arranged by size:
3889 257 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4357
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 194 1171 1286 2016 2109 3165 194
Size: 977 115 730 93 1056 1420
Fragments arranged by size:
1420 1056 977 730 115 93
BmgBI CAC'GTC
Cuts at: 1190 1190
Size: 4391
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 483 2758 483
Size: 2275 2116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 762
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1567 2446 2938 3236 1567
Size: 879 492 298 2722
Fragments arranged by size:
2722 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 312 1290 4095 312
Size: 978 2805 608
Fragments arranged by size:
2805 978 608
BsaAI yAC'GTr
Cuts at: 1744 1744
Size: 4391
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 860 1029 1822 2070 2772 4268 860
Size: 169 793 248 702 1496 983
Fragments arranged by size:
1496 983 793 702 248 169
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 388 418 1363 1393 388
Size: 30 945 30 3386
Fragments arranged by size:
3386 945 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 177 723 916 1384 177
Size: 546 193 468 3184
Fragments arranged by size:
3184 546 468 193
BseYI C'CCAG_C
Cuts at: 332 1816 2574 3151 3647 332
Size: 1484 758 577 496 1076
Fragments arranged by size:
1484 1076 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 499 584 289
Size: 210 85 4096
Fragments arranged by size:
4096 210 85
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3187 104
Size: 1829 46 1208 1308
Fragments arranged by size:
1829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 757 817 1384 1405 2183 2373 3165 757
Size: 60 567 21 778 190 792 1983
Fragments arranged by size:
1983 792 778 567 190 60 21
Bsp1286I G_dGCh'C
Cuts at: 194 757 817 1171 1286 1384 1405 2016 2109
Size: 563 60 354 115 98 21 611 93
Cuts at: 2109 2183 2373 2435 3165 194
Size: 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 563 354 190 115 98
93 74 62 60 21
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2683 2737 4221 4386 50
Size: 2633 54 1484 165 55
Fragments arranged by size:
2633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1124 2303 1124
Size: 1179 3212
BsrFI r'CCGG_y
Cuts at: 336 1025 2389 2570 2779 336
Size: 689 1364 181 209 1948
Fragments arranged by size:
1948 1364 689 209 181
BssSI C'ACGA_G
Cuts at: 2662 3020 2662
Size: 358 4033
BstAPI GCAn_nnn'nTGC
Cuts at: 1768 1768
Size: 4391
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 856 856
Size: 4391
BstYI r'GATC_y
Cuts at: 1 181 1416 1883 1906 2241 2487 3499 3585
Size: 180 1235 467 23 335 246 1012 86
Cuts at: 3585 3597 3943 1
Size: 12 346 449
Fragments arranged by size:
1235 1012 467 449 346 335 246 180
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1006 2341 2522 2685 1006
Size: 1335 181 163 2712
Fragments arranged by size:
2712 1335 181 163
BtsI GCAGTG_nn'
Cuts at: 546 850 1634 546
Size: 304 784 3303
Fragments arranged by size:
3303 784 304
DraI TTT'AAA
Cuts at: 296 1390 296
Size: 1094 3297
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 227 1976 2150 2541 2568 3913 227
Size: 1749 174 391 27 1345 705
Fragments arranged by size:
1749 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 310 583 637 671 745 893 2414 2624 310
Size: 273 54 34 74 148 1521 210 2077
Fragments arranged by size:
2077 1521 273 210 148 74 54 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 616 941 2599 2909 3055 4201 616
Size: 325 1658 310 146 1146 806
Fragments arranged by size:
1658 1146 806 325 310 146
EcoICRI GAG'CTC
Cuts at: 815 1403 815
Size: 588 3803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 762 1028 2216 2648 3395 3663 762
Size: 266 1188 432 747 268 1490
Fragments arranged by size:
1490 1188 747 432 268 266
EcoNI CCTnn'n_nnAGG
Cuts at: 238 238
Size: 4391
EcoO109I rG'GnC_Cy
Cuts at: 1196 1530 1196
Size: 334 4057
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 355 863 1825 2073 3095 4074 355
Size: 508 962 248 1022 979 672
Fragments arranged by size:
1022 979 962 672 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 311 343 968 1000 1113 1145 2233
Size: 32 80 32 625 32 113 32 1088
Cuts at: 2233 2263 2265 2295 199
Size: 30 2 30 2295
Fragments arranged by size:
2295 1088 625 113 80 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1062 1430 1062
Size: 368 4023
HindIII A'AGCT_T
Cuts at: 373 373
Size: 4391
KasI G'GCGC_C
Cuts at: 859 1821 2069 859
Size: 962 248 3181
Fragments arranged by size:
3181 962 248
KpnI G_GTAC'C
Cuts at: 343 1411 343
Size: 1068 3323
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3246 3062
Size: 184 4207
MscI TGG'CCA
Cuts at: 2152 2152
Size: 4391
MslI CAynn'nnrTG
Cuts at: 1306 1363 2380 2507 1306
Size: 57 1017 127 3190
Fragments arranged by size:
3190 1017 127 57
MspA1I CmG'CkG
Cuts at: 792 1502 1816 2176 3189 3434 792
Size: 710 314 360 1013 245 1749
Fragments arranged by size:
1749 1013 710 360 314 245
NaeI GCC'GGC
Cuts at: 1027 2572 1027
Size: 1545 2846
NarI GG'CG_CC
Cuts at: 860 1822 2070 860
Size: 962 248 3181
Fragments arranged by size:
3181 962 248
NgoMIV G'CCGG_C
Cuts at: 1025 2570 1025
Size: 1545 2846
NspI r_CATG'y
Cuts at: 303 766 1242 1444 1944 2851 303
Size: 463 476 202 500 907 1843
Fragments arranged by size:
1843 907 500 476 463 202
PciI A'CATG_T
Cuts at: 2847 2847
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 707 739 3530 3562 707
Size: 32 2791 32 1536
Fragments arranged by size:
2791 1536 32 32
PpuMI rG'GwC_Cy
Cuts at: 1196 1196
Size: 4391
PshAI GACnn'nnGTC
Cuts at: 616 1302 616
Size: 686 3705
PstI C_TGCA'G
Cuts at: 995 1438 2123 995
Size: 443 685 3263
Fragments arranged by size:
3263 685 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 817 1405 817
Size: 588 3803
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 583 745 2414 2624 583
Size: 162 1669 210 2350
Fragments arranged by size:
2350 1669 210 162
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 991 1434 2119 3112 3303 33
Size: 958 443 685 993 191 1121
Fragments arranged by size:
1121 993 958 685 443 191
SfoI GGC'GCC
Cuts at: 861 1823 2071 861
Size: 962 248 3181
Fragments arranged by size:
3181 962 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SgrAI Cr'CCGG_yG
Cuts at: 1025 1025
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 1546 2461 2953 3215 1546
Size: 915 492 262 2722
Fragments arranged by size:
2722 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1242 1444 1944 1242
Size: 202 500 3689
Fragments arranged by size:
3689 500 202
StyI C'CwwG_G
Cuts at: 344 530 593 1525 1879 344
Size: 186 63 932 354 2856
Fragments arranged by size:
2856 932 354 186 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 1367 1974 2107 2566 2775 346
Size: 1021 607 133 459 209 1962
Fragments arranged by size:
1962 1021 607 459 209 133
TatI w'GTAC_w
Cuts at: 1313 3906 1313
Size: 2593 1798
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 822 854 1193 1225 1363 1395 3908 3940 822
Size: 32 339 32 138 32 2513 32 1273
Fragments arranged by size:
2513 1273 339 138 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1886 1886
Size: 4391
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 1081 1081
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI ZraI