Restriction map for
pF1KB7032
(Circular) (Six-base) MAPSORT of: pF1KB7032.seq Check: 1655 from: 1 to: 4385
pF1KB7032 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1326 1401 1326
Size: 75 4310
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 2210 2642 3389 3657 836
Size: 1374 432 747 268 1564
Fragments arranged by size:
1564 1374 747 432 268
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AleI CACnn'nnGTG
Cuts at: 298 298
Size: 4385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 376
Size: 4385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1300 1332 1300
Size: 32 4353
AlwNI CAG_nnn'CTG
Cuts at: 955 1690 3257 955
Size: 735 1567 2083
Fragments arranged by size:
2083 1567 735
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1129 1239 1389 3738 3994 1129
Size: 110 150 2349 256 1520
Fragments arranged by size:
2349 1520 256 150 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1172 1405 1172
Size: 233 4152
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 314 1326 1401 1815 2063 2098 314
Size: 1012 75 414 248 35 2601
Fragments arranged by size:
2601 1012 414 248 75 35
BanII G_rGCy'C
Cuts at: 1399 2429 1399
Size: 1030 3355
BbeI G_GCGC'C
Cuts at: 318 1819 2067 318
Size: 1501 248 2636
Fragments arranged by size:
2636 1501 248
BbvCI CC'TCA_GC
Cuts at: 1052 1052
Size: 4385
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 608 764 902 1905 608
Size: 156 138 1003 3088
Fragments arranged by size:
3088 1003 156 138
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 1173 2010 2103 3159 1173
Size: 837 93 1056 2399
Fragments arranged by size:
2399 1056 837 93
BmrI ACTGGGnnnn_n'
Cuts at: 1597 1678 2013 4157 1597
Size: 81 335 2144 1825
Fragments arranged by size:
2144 1825 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 2752 214
Size: 2538 1847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 1334 574
Size: 760 3625
Bpu10I CC'TnA_GC
Cuts at: 653 1052 653
Size: 399 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 2932 3230 1561
Size: 879 492 298 2716
Fragments arranged by size:
2716 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 185 4089 185
Size: 3904 481
BsaAI yAC'GTr
Cuts at: 915 1738 915
Size: 823 3562
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 315 1816 2064 2766 4262 315
Size: 1501 248 702 1496 438
Fragments arranged by size:
1501 1496 702 438 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 1069 1299 151
Size: 918 230 3237
Fragments arranged by size:
3237 918 230
BseYI C'CCAG_C
Cuts at: 1810 2568 3145 3641 1810
Size: 758 577 496 2554
Fragments arranged by size:
2554 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1185 1185
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
BsmI GAATG_Cn'
Cuts at: 565 565
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 1173 1399 2010 2103 2177 2367 2429 3159 1173
Size: 226 611 93 74 190 62 730 2399
Fragments arranged by size:
2399 730 611 226 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 641 3561 641
Size: 2920 1465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 1435 1649 1951 2332 179
Size: 1256 214 302 381 2232
Fragments arranged by size:
2232 1256 381 302 214
BsrBI CCG'CTC
Cuts at: 50 227 2677 2731 4215 4380 50
Size: 177 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2297 1046
Size: 1251 3134
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BssHII G'CGCG_C
Cuts at: 151 151
Size: 4385
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 725 1762 725
Size: 1037 3348
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstEII G'GTnAC_C
Cuts at: 745 745
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 300 300
Size: 4385
BstYI r'GATC_y
Cuts at: 1 1058 1410 1877 1900 2235 2481 3493 3579
Size: 1057 352 467 23 335 246 1012 86
Cuts at: 3579 3591 3937 1
Size: 12 346 449
Fragments arranged by size:
1057 1012 467 449 352 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 751 751
Size: 4385
BtgI C'CryG_G
Cuts at: 399 1159 399
Size: 760 3625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 141 201 2335 2516 2679 141
Size: 60 2134 181 163 1847
Fragments arranged by size:
2134 1847 181 163 60
BtsI GCAGTG_nn'
Cuts at: 1173 1628 1173
Size: 455 3930
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DraIII CAC_nnn'GTG
Cuts at: 464 464
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 979 2091 2949 979
Size: 1112 858 2415
Fragments arranged by size:
2415 1112 858
EaeI y'GGCC_r
Cuts at: 113 569 962 1970 2144 2535 2562 3907 113
Size: 456 393 1008 174 391 27 1345 591
Fragments arranged by size:
1345 1008 591 456 393 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 370 508 2408 2618 370
Size: 138 1900 210 2137
Fragments arranged by size:
2137 1900 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 836 1334 2210 2642 3389 3657 574
Size: 262 498 876 432 747 268 1302
Fragments arranged by size:
1302 876 747 498 432 268 262
EcoO109I rG'GnC_Cy
Cuts at: 230 1524 230
Size: 1294 3091
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 744 2404 744
Size: 1660 2725
FspI TGC'GCA
Cuts at: 175 784 2166 175
Size: 609 1382 2394
Fragments arranged by size:
2394 1382 609
FspAI rTGC'GCAy
Cuts at: 784 784
Size: 4385
HaeII r_GCGC'y
Cuts at: 318 1819 2067 3089 4068 318
Size: 1501 248 1022 979 635
Fragments arranged by size:
1501 1022 979 635 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2227 2257 2259 2289 2227
Size: 30 2 30 4323
Fragments arranged by size:
4323 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 314 1815 2063 314
Size: 1501 248 2636
Fragments arranged by size:
2636 1501 248
KpnI G_GTAC'C
Cuts at: 1330 1405 1330
Size: 75 4310
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3240 3056
Size: 184 4201
MscI TGG'CCA
Cuts at: 115 571 964 2146 115
Size: 456 393 1182 2354
Fragments arranged by size:
2354 1182 456 393
MslI CAynn'nnrTG
Cuts at: 298 640 790 2374 2501 298
Size: 342 150 1584 127 2182
Fragments arranged by size:
2182 1584 342 150 127
MspA1I CmG'CkG
Cuts at: 356 1496 1810 2170 3183 3428 356
Size: 1140 314 360 1013 245 1313
Fragments arranged by size:
1313 1140 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 315 1816 2064 315
Size: 1501 248 2636
Fragments arranged by size:
2636 1501 248
NcoI C'CATG_G
Cuts at: 399 399
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 1438 1938 2845 1438
Size: 500 907 2978
Fragments arranged by size:
2978 907 500
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PflMI CCAn_nnn'nTGG
Cuts at: 324 975 324
Size: 651 3734
PfoI T'CCnGG_A
Cuts at: 131 131
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PmlI CAC'GTG
Cuts at: 915 915
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PshAI GACnn'nnGTC
Cuts at: 475 475
Size: 4385
PstI C_TGCA'G
Cuts at: 355 1432 2117 355
Size: 1077 685 2623
Fragments arranged by size:
2623 1077 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 356 1810 2170 356
Size: 1454 360 2571
Fragments arranged by size:
2571 1454 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 370 2408 2618 370
Size: 2038 210 2137
Fragments arranged by size:
2137 2038 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 277 351 1428 2113 3106 3297 33
Size: 244 74 1077 685 993 191 1121
Fragments arranged by size:
1121 1077 993 685 244 191 74
SfoI GGC'GCC
Cuts at: 316 1817 2065 316
Size: 1501 248 2636
Fragments arranged by size:
2636 1501 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 1540 2455 2947 3209 1540
Size: 915 492 262 2716
Fragments arranged by size:
2716 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
SspI AAT'ATT
Cuts at: 597 597
Size: 4385
StyI C'CwwG_G
Cuts at: 296 399 1519 1873 296
Size: 103 1120 354 2808
Fragments arranged by size:
2808 1120 354 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 1968 2101 2560 2769 733
Size: 1235 133 459 209 2349
Fragments arranged by size:
2349 1235 459 209 133
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 972 1880 972
Size: 908 3477
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 1275 1275
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BstZ17I ClaI CspCI EcoNI EcoRV FseI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI